Amino acid dipepetide frequency for Lactococcus phage SK1 (Lactococcus lactis bacteriophage SK1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.355AlaAla: 0.355 ± 0.146
0.118AlaCys: 0.118 ± 0.122
3.196AlaAsp: 3.196 ± 0.582
4.38AlaGlu: 4.38 ± 0.882
3.078AlaPhe: 3.078 ± 0.738
5.09AlaGly: 5.09 ± 1.077
0.947AlaHis: 0.947 ± 0.358
4.972AlaIle: 4.972 ± 1.066
5.208AlaLys: 5.208 ± 0.909
7.339AlaLeu: 7.339 ± 0.89
2.131AlaMet: 2.131 ± 0.614
4.143AlaAsn: 4.143 ± 0.864
0.592AlaPro: 0.592 ± 0.271
2.486AlaGln: 2.486 ± 0.544
1.894AlaArg: 1.894 ± 0.453
3.788AlaSer: 3.788 ± 0.777
2.723AlaThr: 2.723 ± 0.763
4.38AlaVal: 4.38 ± 0.943
1.776AlaTrp: 1.776 ± 0.798
1.42AlaTyr: 1.42 ± 0.37
0.0AlaXaa: 0.0 ± 0.0
Cys
0.237CysAla: 0.237 ± 0.175
0.0CysCys: 0.0 ± 0.0
0.355CysAsp: 0.355 ± 0.195
0.355CysGlu: 0.355 ± 0.216
0.237CysPhe: 0.237 ± 0.148
1.065CysGly: 1.065 ± 0.441
0.355CysHis: 0.355 ± 0.198
0.473CysIle: 0.473 ± 0.285
0.71CysLys: 0.71 ± 0.321
0.355CysLeu: 0.355 ± 0.212
0.118CysMet: 0.118 ± 0.144
0.473CysAsn: 0.473 ± 0.248
0.355CysPro: 0.355 ± 0.212
0.237CysGln: 0.237 ± 0.163
0.592CysArg: 0.592 ± 0.253
0.473CysSer: 0.473 ± 0.278
0.118CysThr: 0.118 ± 0.136
0.355CysVal: 0.355 ± 0.196
0.118CysTrp: 0.118 ± 0.106
0.473CysTyr: 0.473 ± 0.27
0.0CysXaa: 0.0 ± 0.0
Asp
1.894AspAla: 1.894 ± 0.475
0.118AspCys: 0.118 ± 0.119
3.551AspAsp: 3.551 ± 0.899
3.788AspGlu: 3.788 ± 0.84
3.67AspPhe: 3.67 ± 0.647
3.433AspGly: 3.433 ± 0.677
0.592AspHis: 0.592 ± 0.311
4.261AspIle: 4.261 ± 0.63
5.445AspLys: 5.445 ± 0.656
6.037AspLeu: 6.037 ± 0.968
1.065AspMet: 1.065 ± 0.292
4.38AspAsn: 4.38 ± 0.794
1.42AspPro: 1.42 ± 0.476
0.592AspGln: 0.592 ± 0.254
1.657AspArg: 1.657 ± 0.464
3.078AspSer: 3.078 ± 0.635
4.498AspThr: 4.498 ± 0.681
3.078AspVal: 3.078 ± 0.661
0.947AspTrp: 0.947 ± 0.401
2.723AspTyr: 2.723 ± 0.505
0.0AspXaa: 0.0 ± 0.0
Glu
3.433GluAla: 3.433 ± 0.49
0.829GluCys: 0.829 ± 0.425
3.314GluAsp: 3.314 ± 0.667
5.563GluGlu: 5.563 ± 1.054
4.143GluPhe: 4.143 ± 0.694
2.012GluGly: 2.012 ± 0.441
0.71GluHis: 0.71 ± 0.323
6.866GluIle: 6.866 ± 1.062
7.221GluLys: 7.221 ± 1.307
11.009GluLeu: 11.009 ± 1.363
2.841GluMet: 2.841 ± 0.549
4.38GluAsn: 4.38 ± 0.727
1.42GluPro: 1.42 ± 0.447
4.498GluGln: 4.498 ± 0.823
3.196GluArg: 3.196 ± 0.589
3.788GluSer: 3.788 ± 0.529
4.853GluThr: 4.853 ± 0.72
4.38GluVal: 4.38 ± 0.786
0.947GluTrp: 0.947 ± 0.335
3.906GluTyr: 3.906 ± 0.888
0.0GluXaa: 0.0 ± 0.0
Phe
3.551PheAla: 3.551 ± 0.772
0.355PheCys: 0.355 ± 0.208
3.314PheAsp: 3.314 ± 0.639
2.841PheGlu: 2.841 ± 0.632
2.249PhePhe: 2.249 ± 0.819
2.604PheGly: 2.604 ± 0.653
0.592PheHis: 0.592 ± 0.216
3.078PheIle: 3.078 ± 0.68
4.616PheLys: 4.616 ± 0.694
2.367PheLeu: 2.367 ± 0.492
1.184PheMet: 1.184 ± 0.343
3.314PheAsn: 3.314 ± 0.677
0.71PhePro: 0.71 ± 0.287
1.065PheGln: 1.065 ± 0.405
1.184PheArg: 1.184 ± 0.287
3.788PheSer: 3.788 ± 0.934
2.841PheThr: 2.841 ± 0.454
2.723PheVal: 2.723 ± 0.496
0.355PheTrp: 0.355 ± 0.206
1.776PheTyr: 1.776 ± 0.412
0.0PheXaa: 0.0 ± 0.0
Gly
3.67GlyAla: 3.67 ± 1.382
0.355GlyCys: 0.355 ± 0.194
2.723GlyAsp: 2.723 ± 0.658
4.616GlyGlu: 4.616 ± 0.695
2.012GlyPhe: 2.012 ± 0.526
4.025GlyGly: 4.025 ± 0.794
0.592GlyHis: 0.592 ± 0.247
4.38GlyIle: 4.38 ± 1.278
6.274GlyLys: 6.274 ± 0.825
5.445GlyLeu: 5.445 ± 1.043
1.302GlyMet: 1.302 ± 0.397
3.551GlyAsn: 3.551 ± 0.607
0.237GlyPro: 0.237 ± 0.17
2.131GlyGln: 2.131 ± 0.511
2.131GlyArg: 2.131 ± 0.44
6.037GlySer: 6.037 ± 1.242
2.486GlyThr: 2.486 ± 0.643
5.445GlyVal: 5.445 ± 1.475
1.42GlyTrp: 1.42 ± 0.335
3.67GlyTyr: 3.67 ± 0.757
0.0GlyXaa: 0.0 ± 0.0
His
0.947HisAla: 0.947 ± 0.34
0.71HisCys: 0.71 ± 0.424
0.473HisAsp: 0.473 ± 0.236
0.947HisGlu: 0.947 ± 0.332
0.592HisPhe: 0.592 ± 0.263
1.065HisGly: 1.065 ± 0.39
0.118HisHis: 0.118 ± 0.136
1.065HisIle: 1.065 ± 0.351
0.947HisLys: 0.947 ± 0.301
0.592HisLeu: 0.592 ± 0.289
0.0HisMet: 0.0 ± 0.0
1.065HisAsn: 1.065 ± 0.362
0.118HisPro: 0.118 ± 0.134
0.355HisGln: 0.355 ± 0.202
0.0HisArg: 0.0 ± 0.0
0.237HisSer: 0.237 ± 0.157
0.829HisThr: 0.829 ± 0.342
0.71HisVal: 0.71 ± 0.26
0.237HisTrp: 0.237 ± 0.221
0.473HisTyr: 0.473 ± 0.25
0.0HisXaa: 0.0 ± 0.0
Ile
4.616IleAla: 4.616 ± 0.637
0.237IleCys: 0.237 ± 0.167
4.616IleAsp: 4.616 ± 0.704
7.576IleGlu: 7.576 ± 1.3
3.078IlePhe: 3.078 ± 0.683
3.314IleGly: 3.314 ± 0.849
0.947IleHis: 0.947 ± 0.312
4.616IleIle: 4.616 ± 0.571
6.155IleLys: 6.155 ± 0.792
5.327IleLeu: 5.327 ± 0.939
1.776IleMet: 1.776 ± 0.546
5.09IleAsn: 5.09 ± 0.612
1.184IlePro: 1.184 ± 0.327
1.894IleGln: 1.894 ± 0.345
2.012IleArg: 2.012 ± 0.532
3.67IleSer: 3.67 ± 0.76
6.155IleThr: 6.155 ± 0.97
4.853IleVal: 4.853 ± 0.697
1.302IleTrp: 1.302 ± 0.476
2.012IleTyr: 2.012 ± 0.596
0.0IleXaa: 0.0 ± 0.0
Lys
6.155LysAla: 6.155 ± 0.895
0.947LysCys: 0.947 ± 0.425
4.38LysAsp: 4.38 ± 0.637
9.351LysGlu: 9.351 ± 1.403
2.841LysPhe: 2.841 ± 0.521
6.274LysGly: 6.274 ± 1.008
0.947LysHis: 0.947 ± 0.441
5.208LysIle: 5.208 ± 0.727
8.641LysLys: 8.641 ± 0.914
6.866LysLeu: 6.866 ± 0.865
3.314LysMet: 3.314 ± 0.467
5.09LysAsn: 5.09 ± 0.666
1.539LysPro: 1.539 ± 0.362
3.551LysGln: 3.551 ± 0.555
3.433LysArg: 3.433 ± 0.643
4.616LysSer: 4.616 ± 0.732
6.037LysThr: 6.037 ± 1.02
6.51LysVal: 6.51 ± 0.821
0.947LysTrp: 0.947 ± 0.296
3.906LysTyr: 3.906 ± 0.685
0.0LysXaa: 0.0 ± 0.0
Leu
5.445LeuAla: 5.445 ± 0.698
0.355LeuCys: 0.355 ± 0.204
4.972LeuAsp: 4.972 ± 0.797
5.8LeuGlu: 5.8 ± 0.892
3.433LeuPhe: 3.433 ± 0.687
4.972LeuGly: 4.972 ± 0.725
1.42LeuHis: 1.42 ± 0.449
7.102LeuIle: 7.102 ± 1.055
8.759LeuLys: 8.759 ± 0.956
6.51LeuLeu: 6.51 ± 1.092
1.539LeuMet: 1.539 ± 0.504
5.09LeuAsn: 5.09 ± 0.939
2.367LeuPro: 2.367 ± 0.499
2.959LeuGln: 2.959 ± 0.435
3.078LeuArg: 3.078 ± 0.55
5.327LeuSer: 5.327 ± 0.669
6.155LeuThr: 6.155 ± 0.747
5.563LeuVal: 5.563 ± 0.704
1.539LeuTrp: 1.539 ± 0.337
4.38LeuTyr: 4.38 ± 0.789
0.0LeuXaa: 0.0 ± 0.0
Met
2.723MetAla: 2.723 ± 0.493
0.0MetCys: 0.0 ± 0.0
1.42MetAsp: 1.42 ± 0.326
2.249MetGlu: 2.249 ± 0.546
0.71MetPhe: 0.71 ± 0.276
0.947MetGly: 0.947 ± 0.291
0.237MetHis: 0.237 ± 0.161
2.486MetIle: 2.486 ± 0.649
2.723MetLys: 2.723 ± 0.633
1.065MetLeu: 1.065 ± 0.356
0.355MetMet: 0.355 ± 0.207
2.131MetAsn: 2.131 ± 0.566
0.355MetPro: 0.355 ± 0.185
1.894MetGln: 1.894 ± 0.409
0.355MetArg: 0.355 ± 0.205
1.894MetSer: 1.894 ± 0.492
1.42MetThr: 1.42 ± 0.43
1.42MetVal: 1.42 ± 0.371
0.118MetTrp: 0.118 ± 0.113
1.539MetTyr: 1.539 ± 0.391
0.0MetXaa: 0.0 ± 0.0
Asn
4.616AsnAla: 4.616 ± 1.041
0.237AsnCys: 0.237 ± 0.161
3.788AsnAsp: 3.788 ± 0.81
5.563AsnGlu: 5.563 ± 1.072
2.249AsnPhe: 2.249 ± 0.572
6.155AsnGly: 6.155 ± 1.021
0.71AsnHis: 0.71 ± 0.262
4.143AsnIle: 4.143 ± 0.584
4.972AsnLys: 4.972 ± 1.18
4.616AsnLeu: 4.616 ± 0.793
1.894AsnMet: 1.894 ± 0.412
3.314AsnAsn: 3.314 ± 0.588
2.249AsnPro: 2.249 ± 0.568
2.131AsnGln: 2.131 ± 0.529
2.012AsnArg: 2.012 ± 0.391
4.261AsnSer: 4.261 ± 0.597
3.906AsnThr: 3.906 ± 0.748
2.959AsnVal: 2.959 ± 0.559
0.947AsnTrp: 0.947 ± 0.348
2.486AsnTyr: 2.486 ± 0.566
0.0AsnXaa: 0.0 ± 0.0
Pro
2.131ProAla: 2.131 ± 0.475
0.0ProCys: 0.0 ± 0.0
1.657ProAsp: 1.657 ± 0.425
1.302ProGlu: 1.302 ± 0.449
1.065ProPhe: 1.065 ± 0.292
0.118ProGly: 0.118 ± 0.104
0.237ProHis: 0.237 ± 0.151
1.657ProIle: 1.657 ± 0.46
2.249ProLys: 2.249 ± 0.543
1.657ProLeu: 1.657 ± 0.404
0.355ProMet: 0.355 ± 0.177
1.894ProAsn: 1.894 ± 0.634
0.473ProPro: 0.473 ± 0.295
0.355ProGln: 0.355 ± 0.245
0.473ProArg: 0.473 ± 0.215
1.065ProSer: 1.065 ± 0.409
2.012ProThr: 2.012 ± 0.421
1.42ProVal: 1.42 ± 0.436
0.0ProTrp: 0.0 ± 0.0
0.71ProTyr: 0.71 ± 0.293
0.0ProXaa: 0.0 ± 0.0
Gln
3.196GlnAla: 3.196 ± 0.775
0.237GlnCys: 0.237 ± 0.178
2.012GlnAsp: 2.012 ± 0.44
2.723GlnGlu: 2.723 ± 0.535
1.302GlnPhe: 1.302 ± 0.379
2.249GlnGly: 2.249 ± 0.535
0.118GlnHis: 0.118 ± 0.105
1.065GlnIle: 1.065 ± 0.278
2.959GlnLys: 2.959 ± 0.707
4.261GlnLeu: 4.261 ± 0.725
0.71GlnMet: 0.71 ± 0.215
2.131GlnAsn: 2.131 ± 0.466
1.302GlnPro: 1.302 ± 0.325
1.42GlnGln: 1.42 ± 0.497
1.302GlnArg: 1.302 ± 0.407
2.249GlnSer: 2.249 ± 0.521
2.367GlnThr: 2.367 ± 0.456
1.894GlnVal: 1.894 ± 0.465
0.71GlnTrp: 0.71 ± 0.228
1.184GlnTyr: 1.184 ± 0.366
0.0GlnXaa: 0.0 ± 0.0
Arg
2.249ArgAla: 2.249 ± 0.665
0.473ArgCys: 0.473 ± 0.225
1.894ArgAsp: 1.894 ± 0.406
2.249ArgGlu: 2.249 ± 0.432
0.829ArgPhe: 0.829 ± 0.26
1.894ArgGly: 1.894 ± 0.361
0.473ArgHis: 0.473 ± 0.238
1.776ArgIle: 1.776 ± 0.492
4.616ArgLys: 4.616 ± 0.883
3.788ArgLeu: 3.788 ± 0.575
0.947ArgMet: 0.947 ± 0.404
2.249ArgAsn: 2.249 ± 0.617
0.829ArgPro: 0.829 ± 0.355
1.42ArgGln: 1.42 ± 0.375
2.131ArgArg: 2.131 ± 0.538
1.539ArgSer: 1.539 ± 0.373
1.776ArgThr: 1.776 ± 0.386
1.776ArgVal: 1.776 ± 0.448
0.355ArgTrp: 0.355 ± 0.182
2.012ArgTyr: 2.012 ± 0.439
0.0ArgXaa: 0.0 ± 0.0
Ser
4.616SerAla: 4.616 ± 1.567
0.829SerCys: 0.829 ± 0.406
3.551SerAsp: 3.551 ± 0.677
4.143SerGlu: 4.143 ± 0.842
3.314SerPhe: 3.314 ± 0.714
4.735SerGly: 4.735 ± 1.3
0.829SerHis: 0.829 ± 0.324
4.616SerIle: 4.616 ± 0.771
5.445SerLys: 5.445 ± 0.79
6.274SerLeu: 6.274 ± 0.79
2.131SerMet: 2.131 ± 0.419
3.078SerAsn: 3.078 ± 0.638
1.302SerPro: 1.302 ± 0.391
1.776SerGln: 1.776 ± 0.488
2.604SerArg: 2.604 ± 0.48
5.445SerSer: 5.445 ± 1.103
2.959SerThr: 2.959 ± 0.688
4.143SerVal: 4.143 ± 0.966
0.829SerTrp: 0.829 ± 0.32
2.249SerTyr: 2.249 ± 0.576
0.0SerXaa: 0.0 ± 0.0
Thr
4.498ThrAla: 4.498 ± 0.676
0.237ThrCys: 0.237 ± 0.184
3.551ThrAsp: 3.551 ± 0.808
6.037ThrGlu: 6.037 ± 0.769
2.841ThrPhe: 2.841 ± 0.611
4.498ThrGly: 4.498 ± 0.926
0.118ThrHis: 0.118 ± 0.113
3.788ThrIle: 3.788 ± 0.746
4.616ThrLys: 4.616 ± 0.691
5.682ThrLeu: 5.682 ± 0.843
0.829ThrMet: 0.829 ± 0.32
4.38ThrAsn: 4.38 ± 0.743
1.776ThrPro: 1.776 ± 0.32
2.604ThrGln: 2.604 ± 0.493
1.894ThrArg: 1.894 ± 0.457
4.616ThrSer: 4.616 ± 0.863
4.38ThrThr: 4.38 ± 0.789
4.38ThrVal: 4.38 ± 1.046
1.302ThrTrp: 1.302 ± 0.509
2.367ThrTyr: 2.367 ± 0.543
0.0ThrXaa: 0.0 ± 0.0
Val
3.314ValAla: 3.314 ± 0.621
0.592ValCys: 0.592 ± 0.213
4.143ValAsp: 4.143 ± 0.596
5.09ValGlu: 5.09 ± 0.708
3.196ValPhe: 3.196 ± 0.596
3.551ValGly: 3.551 ± 0.585
0.237ValHis: 0.237 ± 0.128
4.38ValIle: 4.38 ± 0.643
5.445ValLys: 5.445 ± 0.761
3.078ValLeu: 3.078 ± 0.428
2.131ValMet: 2.131 ± 0.465
2.604ValAsn: 2.604 ± 0.528
1.539ValPro: 1.539 ± 0.526
2.367ValGln: 2.367 ± 0.611
3.196ValArg: 3.196 ± 0.666
5.327ValSer: 5.327 ± 1.181
5.327ValThr: 5.327 ± 0.851
3.788ValVal: 3.788 ± 0.669
0.237ValTrp: 0.237 ± 0.136
3.078ValTyr: 3.078 ± 0.588
0.0ValXaa: 0.0 ± 0.0
Trp
0.829TrpAla: 0.829 ± 0.284
0.118TrpCys: 0.118 ± 0.13
0.71TrpAsp: 0.71 ± 0.462
0.473TrpGlu: 0.473 ± 0.244
0.947TrpPhe: 0.947 ± 0.353
1.302TrpGly: 1.302 ± 0.404
0.355TrpHis: 0.355 ± 0.228
0.71TrpIle: 0.71 ± 0.385
0.947TrpLys: 0.947 ± 0.308
1.184TrpLeu: 1.184 ± 0.446
0.237TrpMet: 0.237 ± 0.165
1.539TrpAsn: 1.539 ± 0.555
0.0TrpPro: 0.0 ± 0.0
0.592TrpGln: 0.592 ± 0.228
0.473TrpArg: 0.473 ± 0.309
1.302TrpSer: 1.302 ± 0.3
0.592TrpThr: 0.592 ± 0.251
0.71TrpVal: 0.71 ± 0.297
0.237TrpTrp: 0.237 ± 0.146
1.184TrpTyr: 1.184 ± 0.351
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.539TyrAla: 1.539 ± 0.496
0.71TyrCys: 0.71 ± 0.348
2.604TyrAsp: 2.604 ± 0.729
4.261TyrGlu: 4.261 ± 0.762
2.723TyrPhe: 2.723 ± 0.536
3.196TyrGly: 3.196 ± 0.608
0.947TyrHis: 0.947 ± 0.299
3.788TyrIle: 3.788 ± 0.749
2.604TyrLys: 2.604 ± 0.549
3.433TyrLeu: 3.433 ± 0.644
1.065TyrMet: 1.065 ± 0.41
3.314TyrAsn: 3.314 ± 0.52
1.065TyrPro: 1.065 ± 0.372
1.065TyrGln: 1.065 ± 0.511
1.657TyrArg: 1.657 ± 0.493
2.367TyrSer: 2.367 ± 0.589
2.959TyrThr: 2.959 ± 0.696
2.131TyrVal: 2.131 ± 0.514
0.118TyrTrp: 0.118 ± 0.136
2.131TyrTyr: 2.131 ± 0.624
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (8449 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski