Cellulomonas carbonis T26

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; Cellulomonas carbonis

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3407 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0BXQ5|A0A0A0BXQ5_9CELL Chemotaxis protein CheY OS=Cellulomonas carbonis T26 OX=947969 GN=N868_11905 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.61MRR4 pKa = 11.84RR5 pKa = 11.84IGAAGAIVATGALALSACSSTPSTTTEE32 pKa = 3.61IDD34 pKa = 3.41EE35 pKa = 4.35EE36 pKa = 4.61TSLSIGWNQPFYY48 pKa = 10.99SQNNLTSVGNATTNSNVIYY67 pKa = 9.01LTNAAFNYY75 pKa = 9.87YY76 pKa = 10.68DD77 pKa = 4.31NNLEE81 pKa = 4.09LQQFSDD87 pKa = 3.51FGTYY91 pKa = 7.97EE92 pKa = 4.08VEE94 pKa = 4.61SEE96 pKa = 4.46DD97 pKa = 4.36PLTVTYY103 pKa = 9.05TINEE107 pKa = 4.16GATWSDD113 pKa = 3.52GTPVDD118 pKa = 3.54AVDD121 pKa = 3.75VFLYY125 pKa = 9.92WGAQNDD131 pKa = 4.03SFDD134 pKa = 3.95NVAPEE139 pKa = 3.8YY140 pKa = 10.76DD141 pKa = 3.58EE142 pKa = 5.14EE143 pKa = 5.35GNITNQDD150 pKa = 3.7AIDD153 pKa = 3.75AGVYY157 pKa = 10.11FDD159 pKa = 4.39SSSVAMNLISEE170 pKa = 4.83PPTISDD176 pKa = 4.25DD177 pKa = 3.76GLSITFVYY185 pKa = 10.46DD186 pKa = 3.48EE187 pKa = 5.15PRR189 pKa = 11.84SDD191 pKa = 3.29WEE193 pKa = 3.98ISVQPSPIAAHH204 pKa = 6.68ALAAVALGTEE214 pKa = 4.43DD215 pKa = 4.7AEE217 pKa = 4.36EE218 pKa = 4.48GKK220 pKa = 10.21QRR222 pKa = 11.84VVDD225 pKa = 4.01AFTNNVTEE233 pKa = 4.86DD234 pKa = 3.62LSALAKK240 pKa = 10.11SWNEE244 pKa = 3.67DD245 pKa = 3.53FNFTSLPEE253 pKa = 4.26DD254 pKa = 3.45EE255 pKa = 5.3NLYY258 pKa = 10.57LSSGPYY264 pKa = 10.56VMTDD268 pKa = 3.32YY269 pKa = 11.61VEE271 pKa = 4.1NQYY274 pKa = 9.01LTLSLRR280 pKa = 11.84EE281 pKa = 4.23DD282 pKa = 4.4YY283 pKa = 11.06SWGPMPSVDD292 pKa = 4.52EE293 pKa = 3.67ITIRR297 pKa = 11.84YY298 pKa = 9.48SEE300 pKa = 5.07DD301 pKa = 3.03PLASVTALQNGEE313 pKa = 3.81VDD315 pKa = 3.41IIEE318 pKa = 4.5PQASVDD324 pKa = 3.91VIEE327 pKa = 4.21TLEE330 pKa = 4.58GIEE333 pKa = 4.04GVEE336 pKa = 4.24YY337 pKa = 8.19TTAPTGTYY345 pKa = 8.83EE346 pKa = 4.54HH347 pKa = 7.15VDD349 pKa = 4.1LIFNNGGPFDD359 pKa = 4.17PATYY363 pKa = 10.55GGDD366 pKa = 3.42AAKK369 pKa = 10.53AQAVRR374 pKa = 11.84QAFLLSVPRR383 pKa = 11.84DD384 pKa = 3.56EE385 pKa = 6.1IIEE388 pKa = 4.32KK389 pKa = 10.13LVQPLQPDD397 pKa = 3.87AEE399 pKa = 4.34PRR401 pKa = 11.84EE402 pKa = 4.4SHH404 pKa = 5.71TVTSEE409 pKa = 3.51APAYY413 pKa = 10.39DD414 pKa = 3.97DD415 pKa = 4.21VVAGNGSEE423 pKa = 4.75FYY425 pKa = 9.71RR426 pKa = 11.84TSDD429 pKa = 3.49PAKK432 pKa = 10.19AAQLLADD439 pKa = 4.81AGVATPIDD447 pKa = 3.52VRR449 pKa = 11.84FLYY452 pKa = 10.46GASNVRR458 pKa = 11.84RR459 pKa = 11.84ANTFEE464 pKa = 4.92LIKK467 pKa = 10.92ASAAEE472 pKa = 3.95AGFNVIDD479 pKa = 4.79EE480 pKa = 5.18GDD482 pKa = 4.17DD483 pKa = 3.38NWGSRR488 pKa = 11.84LSDD491 pKa = 3.27TASYY495 pKa = 10.52DD496 pKa = 3.06ASLFGWQSTNTYY508 pKa = 10.53ALNSEE513 pKa = 4.56ANFVTGGLNNFAGMSDD529 pKa = 3.69PDD531 pKa = 3.07IDD533 pKa = 3.03AWYY536 pKa = 10.88AEE538 pKa = 4.49MSTNTDD544 pKa = 3.39PEE546 pKa = 4.32QEE548 pKa = 4.23TEE550 pKa = 4.0LTILIEE556 pKa = 4.06EE557 pKa = 4.49RR558 pKa = 11.84LNEE561 pKa = 4.31LGFSLPLYY569 pKa = 7.37QHH571 pKa = 7.06PGVMAYY577 pKa = 9.95RR578 pKa = 11.84DD579 pKa = 3.53SLQNVEE585 pKa = 5.0TIALSPTVFWNFWEE599 pKa = 4.38WEE601 pKa = 4.32VGGDD605 pKa = 3.72TADD608 pKa = 3.69EE609 pKa = 4.22EE610 pKa = 4.64

Molecular weight: 66.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0BXG6|A0A0A0BXG6_9CELL Ribonucleoside-triphosphate reductase OS=Cellulomonas carbonis T26 OX=947969 GN=N868_07230 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.97GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3407

0

3407

1114276

35

1968

327.1

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.316 ± 0.055

0.593 ± 0.01

6.591 ± 0.035

5.6 ± 0.044

2.42 ± 0.026

9.481 ± 0.039

2.186 ± 0.02

2.556 ± 0.032

1.244 ± 0.027

10.251 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.608 ± 0.016

1.379 ± 0.022

5.955 ± 0.036

2.425 ± 0.021

8.307 ± 0.045

4.722 ± 0.024

6.319 ± 0.038

10.805 ± 0.051

1.497 ± 0.02

1.741 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski