Bat polyomavirus
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3MCH7|A0A0D3MCH7_9POLY Capsid protein VP1 OS=Bat polyomavirus OX=1221637 PE=3 SV=1
MM1 pKa = 7.32 GAFLAVLAEE10 pKa = 4.12 VFEE13 pKa = 4.94 LSTVTGLSVEE23 pKa = 4.57 TILSGEE29 pKa = 4.19 AFTTAEE35 pKa = 4.49 LLQSHH40 pKa = 7.16 IANLVTFGGLTEE52 pKa = 4.58 AEE54 pKa = 3.95 ALAAAEE60 pKa = 4.44 VSAEE64 pKa = 4.14 AYY66 pKa = 9.4 SALSTLSEE74 pKa = 4.26 TFPQAFATLAATEE87 pKa = 4.17 FATTGTLTVGAAIAAALYY105 pKa = 9.05 PYY107 pKa = 10.11 YY108 pKa = 10.41 YY109 pKa = 9.96 DD110 pKa = 3.53 YY111 pKa = 11.49 SVPISNLNRR120 pKa = 11.84 SMALQQWIPDD130 pKa = 3.67 YY131 pKa = 11.47 DD132 pKa = 4.39 LDD134 pKa = 4.33 FPGVRR139 pKa = 11.84 PLVRR143 pKa = 11.84 FLNYY147 pKa = 9.28 IDD149 pKa = 5.05 PYY151 pKa = 9.41 TWASDD156 pKa = 3.75 LYY158 pKa = 10.54 HH159 pKa = 7.21 SIGRR163 pKa = 11.84 YY164 pKa = 7.36 FWEE167 pKa = 3.99 TAQRR171 pKa = 11.84 IGQNVIEE178 pKa = 4.87 EE179 pKa = 4.45 EE180 pKa = 4.26 LTDD183 pKa = 3.73 LTRR186 pKa = 11.84 DD187 pKa = 3.3 LAVRR191 pKa = 11.84 SVTSISEE198 pKa = 4.22 MIAHH202 pKa = 6.01 YY203 pKa = 10.34 FEE205 pKa = 4.03 NARR208 pKa = 11.84 WAVSLLPRR216 pKa = 11.84 SIYY219 pKa = 10.73 NSLEE223 pKa = 3.92 TYY225 pKa = 8.7 YY226 pKa = 10.86 TEE228 pKa = 4.99 LPGINPIQARR238 pKa = 11.84 QLYY241 pKa = 8.75 RR242 pKa = 11.84 KK243 pKa = 10.03 LGEE246 pKa = 4.25 TPIHH250 pKa = 7.02 RR251 pKa = 11.84 GRR253 pKa = 11.84 GPGGPARR260 pKa = 11.84 VRR262 pKa = 11.84 RR263 pKa = 11.84 SPYY266 pKa = 9.93 LGRR269 pKa = 11.84 GPGGPATIFGNIQGGAVPQQFSAQYY294 pKa = 9.04 VEE296 pKa = 5.64 RR297 pKa = 11.84 YY298 pKa = 7.17 PPPGGADD305 pKa = 2.91 QRR307 pKa = 11.84 TAADD311 pKa = 3.13 WMLPLILGLYY321 pKa = 10.37 GDD323 pKa = 5.15 ISPSWRR329 pKa = 11.84 RR330 pKa = 11.84 TLEE333 pKa = 4.14 DD334 pKa = 3.88 LEE336 pKa = 4.44 EE337 pKa = 4.75 EE338 pKa = 4.1 EE339 pKa = 6.42 DD340 pKa = 4.22 GPQKK344 pKa = 10.53 KK345 pKa = 9.6 KK346 pKa = 10.29 PRR348 pKa = 11.84 RR349 pKa = 11.84 AA350 pKa = 3.22
Molecular weight: 38.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.802
IPC2_protein 4.914
IPC_protein 4.825
Toseland 4.711
ProMoST 4.914
Dawson 4.762
Bjellqvist 4.914
Wikipedia 4.609
Rodwell 4.685
Grimsley 4.622
Solomon 4.762
Lehninger 4.724
Nozaki 4.876
DTASelect 4.978
Thurlkill 4.698
EMBOSS 4.635
Sillero 4.952
Patrickios 3.605
IPC_peptide 4.774
IPC2_peptide 4.952
IPC2.peptide.svr19 4.893
Protein with the highest isoelectric point:
>tr|A0A0D3MDQ1|A0A0D3MDQ1_9POLY Large T antigen OS=Bat polyomavirus OX=1221637 PE=4 SV=1
MM1 pKa = 7.85 DD2 pKa = 3.57 HH3 pKa = 6.67 TLTRR7 pKa = 11.84 EE8 pKa = 3.46 EE9 pKa = 4.37 SLRR12 pKa = 11.84 LMEE15 pKa = 5.3 LLSLPMEE22 pKa = 4.35 QYY24 pKa = 11.84 GNFPLMRR31 pKa = 11.84 KK32 pKa = 9.48 AFLKK36 pKa = 10.02 KK37 pKa = 10.34 CKK39 pKa = 9.55 ILHH42 pKa = 6.42 PDD44 pKa = 2.78 KK45 pKa = 11.36 GGNQEE50 pKa = 4.08 LAKK53 pKa = 10.45 EE54 pKa = 4.36 LISLYY59 pKa = 10.52 KK60 pKa = 10.27 RR61 pKa = 11.84 LEE63 pKa = 4.09 EE64 pKa = 4.17 MLPTLNPEE72 pKa = 4.14 EE73 pKa = 4.55 SFSTSQVHH81 pKa = 5.85 EE82 pKa = 4.36 SSDD85 pKa = 3.45 LFIWMDD91 pKa = 3.73 DD92 pKa = 3.25 WPKK95 pKa = 11.02 CKK97 pKa = 9.86 IGANPTCRR105 pKa = 11.84 CLFCLLQRR113 pKa = 11.84 NHH115 pKa = 6.59 RR116 pKa = 11.84 KK117 pKa = 9.37 RR118 pKa = 11.84 RR119 pKa = 11.84 TPWPKK124 pKa = 9.25 VWGDD128 pKa = 3.59 CFCFRR133 pKa = 11.84 CYY135 pKa = 9.21 ITWFGLEE142 pKa = 4.03 NSWMVFLSWKK152 pKa = 9.59 EE153 pKa = 3.9 IVGKK157 pKa = 8.41 TPYY160 pKa = 9.94 RR161 pKa = 11.84 VLNII165 pKa = 3.89
Molecular weight: 19.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.555
IPC2_protein 7.79
IPC_protein 7.658
Toseland 7.205
ProMoST 8.229
Dawson 8.361
Bjellqvist 8.785
Wikipedia 8.273
Rodwell 8.375
Grimsley 7.176
Solomon 8.463
Lehninger 8.492
Nozaki 9.121
DTASelect 8.463
Thurlkill 8.536
EMBOSS 8.58
Sillero 8.887
Patrickios 4.469
IPC_peptide 8.463
IPC2_peptide 7.863
IPC2.peptide.svr19 7.892
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1540
165
657
385.0
43.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.558 ± 1.349
2.143 ± 0.624
4.935 ± 0.544
6.948 ± 0.36
5.26 ± 0.923
6.039 ± 0.909
1.688 ± 0.26
5.0 ± 0.193
5.714 ± 1.386
10.455 ± 0.593
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.468 ± 0.402
4.416 ± 0.596
6.299 ± 0.623
4.675 ± 0.493
5.0 ± 0.656
5.779 ± 0.31
6.169 ± 0.381
5.519 ± 0.682
1.558 ± 0.424
3.377 ± 0.723
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here