Amino acid dipepetide frequency for Streptomyces sp. CB01249

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.944AlaAla: 21.944 ± 0.167
1.045AlaCys: 1.045 ± 0.024
8.585AlaAsp: 8.585 ± 0.062
8.824AlaGlu: 8.824 ± 0.081
3.592AlaPhe: 3.592 ± 0.046
13.323AlaGly: 13.323 ± 0.086
2.938AlaHis: 2.938 ± 0.043
3.331AlaIle: 3.331 ± 0.048
2.961AlaLys: 2.961 ± 0.053
14.989AlaLeu: 14.989 ± 0.128
2.56AlaMet: 2.56 ± 0.034
1.877AlaAsn: 1.877 ± 0.029
7.691AlaPro: 7.691 ± 0.085
3.681AlaGln: 3.681 ± 0.044
10.316AlaArg: 10.316 ± 0.096
6.011AlaSer: 6.011 ± 0.062
7.105AlaThr: 7.105 ± 0.074
12.46AlaVal: 12.46 ± 0.107
1.864AlaTrp: 1.864 ± 0.03
2.97AlaTyr: 2.97 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
1.076CysAla: 1.076 ± 0.024
0.09CysCys: 0.09 ± 0.006
0.458CysAsp: 0.458 ± 0.016
0.386CysGlu: 0.386 ± 0.014
0.2CysPhe: 0.2 ± 0.01
0.949CysGly: 0.949 ± 0.021
0.185CysHis: 0.185 ± 0.009
0.151CysIle: 0.151 ± 0.008
0.113CysLys: 0.113 ± 0.007
0.692CysLeu: 0.692 ± 0.018
0.126CysMet: 0.126 ± 0.008
0.134CysAsn: 0.134 ± 0.008
0.443CysPro: 0.443 ± 0.015
0.131CysGln: 0.131 ± 0.007
0.564CysArg: 0.564 ± 0.016
0.423CysSer: 0.423 ± 0.016
0.529CysThr: 0.529 ± 0.015
0.68CysVal: 0.68 ± 0.02
0.127CysTrp: 0.127 ± 0.008
0.157CysTyr: 0.157 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
8.216AspAla: 8.216 ± 0.07
0.434AspCys: 0.434 ± 0.014
3.626AspAsp: 3.626 ± 0.043
3.935AspGlu: 3.935 ± 0.046
1.678AspPhe: 1.678 ± 0.032
6.66AspGly: 6.66 ± 0.069
1.374AspHis: 1.374 ± 0.025
1.941AspIle: 1.941 ± 0.035
1.218AspLys: 1.218 ± 0.034
6.171AspLeu: 6.171 ± 0.053
0.806AspMet: 0.806 ± 0.018
0.993AspAsn: 0.993 ± 0.022
4.703AspPro: 4.703 ± 0.056
1.483AspGln: 1.483 ± 0.029
5.041AspArg: 5.041 ± 0.056
2.529AspSer: 2.529 ± 0.034
3.373AspThr: 3.373 ± 0.041
4.524AspVal: 4.524 ± 0.048
1.04AspTrp: 1.04 ± 0.024
1.157AspTyr: 1.157 ± 0.027
0.0AspXaa: 0.0 ± 0.0
Glu
7.648GluAla: 7.648 ± 0.082
0.364GluCys: 0.364 ± 0.014
2.986GluAsp: 2.986 ± 0.042
3.561GluGlu: 3.561 ± 0.057
1.395GluPhe: 1.395 ± 0.027
4.444GluGly: 4.444 ± 0.048
1.429GluHis: 1.429 ± 0.025
2.173GluIle: 2.173 ± 0.038
1.46GluLys: 1.46 ± 0.035
6.678GluLeu: 6.678 ± 0.07
0.856GluMet: 0.856 ± 0.02
1.053GluAsn: 1.053 ± 0.022
3.467GluPro: 3.467 ± 0.046
2.106GluGln: 2.106 ± 0.034
5.286GluArg: 5.286 ± 0.06
2.531GluSer: 2.531 ± 0.036
3.012GluThr: 3.012 ± 0.038
4.337GluVal: 4.337 ± 0.045
0.741GluTrp: 0.741 ± 0.017
1.058GluTyr: 1.058 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.72PheAla: 3.72 ± 0.043
0.257PheCys: 0.257 ± 0.01
1.999PheAsp: 1.999 ± 0.031
1.39PheGlu: 1.39 ± 0.025
0.884PhePhe: 0.884 ± 0.025
3.01PheGly: 3.01 ± 0.041
0.633PheHis: 0.633 ± 0.015
0.748PheIle: 0.748 ± 0.02
0.532PheLys: 0.532 ± 0.017
2.6PheLeu: 2.6 ± 0.041
0.412PheMet: 0.412 ± 0.015
0.613PheAsn: 0.613 ± 0.02
1.404PhePro: 1.404 ± 0.028
0.661PheGln: 0.661 ± 0.017
1.844PheArg: 1.844 ± 0.026
1.462PheSer: 1.462 ± 0.025
2.032PheThr: 2.032 ± 0.038
2.157PheVal: 2.157 ± 0.032
0.424PheTrp: 0.424 ± 0.013
0.614PheTyr: 0.614 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.682GlyAla: 11.682 ± 0.092
0.835GlyCys: 0.835 ± 0.021
5.487GlyAsp: 5.487 ± 0.055
5.168GlyGlu: 5.168 ± 0.055
2.909GlyPhe: 2.909 ± 0.039
8.907GlyGly: 8.907 ± 0.081
2.323GlyHis: 2.323 ± 0.035
3.489GlyIle: 3.489 ± 0.044
2.571GlyLys: 2.571 ± 0.048
9.728GlyLeu: 9.728 ± 0.083
2.01GlyMet: 2.01 ± 0.03
1.858GlyAsn: 1.858 ± 0.035
5.395GlyPro: 5.395 ± 0.067
2.581GlyGln: 2.581 ± 0.038
7.721GlyArg: 7.721 ± 0.081
5.436GlySer: 5.436 ± 0.053
6.417GlyThr: 6.417 ± 0.061
7.34GlyVal: 7.34 ± 0.065
1.689GlyTrp: 1.689 ± 0.03
2.413GlyTyr: 2.413 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.65HisAla: 2.65 ± 0.037
0.19HisCys: 0.19 ± 0.009
1.291HisAsp: 1.291 ± 0.026
1.186HisGlu: 1.186 ± 0.021
0.635HisPhe: 0.635 ± 0.016
2.361HisGly: 2.361 ± 0.036
0.693HisHis: 0.693 ± 0.022
0.707HisIle: 0.707 ± 0.017
0.33HisLys: 0.33 ± 0.014
2.358HisLeu: 2.358 ± 0.034
0.325HisMet: 0.325 ± 0.012
0.37HisAsn: 0.37 ± 0.013
1.823HisPro: 1.823 ± 0.031
0.615HisGln: 0.615 ± 0.018
2.099HisArg: 2.099 ± 0.036
0.99HisSer: 0.99 ± 0.022
1.345HisThr: 1.345 ± 0.028
1.559HisVal: 1.559 ± 0.028
0.377HisTrp: 0.377 ± 0.012
0.474HisTyr: 0.474 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.801IleAla: 4.801 ± 0.057
0.284IleCys: 0.284 ± 0.012
2.222IleAsp: 2.222 ± 0.034
2.015IleGlu: 2.015 ± 0.035
0.695IlePhe: 0.695 ± 0.02
3.708IleGly: 3.708 ± 0.048
0.578IleHis: 0.578 ± 0.017
0.893IleIle: 0.893 ± 0.024
0.76IleLys: 0.76 ± 0.023
2.345IleLeu: 2.345 ± 0.036
0.423IleMet: 0.423 ± 0.013
0.688IleAsn: 0.688 ± 0.019
1.722IlePro: 1.722 ± 0.025
0.736IleGln: 0.736 ± 0.019
2.119IleArg: 2.119 ± 0.033
1.628IleSer: 1.628 ± 0.025
2.155IleThr: 2.155 ± 0.036
2.621IleVal: 2.621 ± 0.043
0.372IleTrp: 0.372 ± 0.012
0.526IleTyr: 0.526 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.973LysAla: 2.973 ± 0.057
0.12LysCys: 0.12 ± 0.007
1.466LysAsp: 1.466 ± 0.034
1.206LysGlu: 1.206 ± 0.029
0.49LysPhe: 0.49 ± 0.014
1.941LysGly: 1.941 ± 0.043
0.437LysHis: 0.437 ± 0.016
0.862LysIle: 0.862 ± 0.023
0.978LysLys: 0.978 ± 0.034
2.115LysLeu: 2.115 ± 0.038
0.392LysMet: 0.392 ± 0.015
0.56LysAsn: 0.56 ± 0.02
1.387LysPro: 1.387 ± 0.032
0.721LysGln: 0.721 ± 0.018
1.403LysArg: 1.403 ± 0.026
1.186LysSer: 1.186 ± 0.028
1.33LysThr: 1.33 ± 0.031
1.883LysVal: 1.883 ± 0.043
0.267LysTrp: 0.267 ± 0.012
0.474LysTyr: 0.474 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.238LeuAla: 15.238 ± 0.122
0.827LeuCys: 0.827 ± 0.021
6.961LeuAsp: 6.961 ± 0.07
4.456LeuGlu: 4.456 ± 0.052
2.629LeuPhe: 2.629 ± 0.043
9.342LeuGly: 9.342 ± 0.073
2.289LeuHis: 2.289 ± 0.034
3.361LeuIle: 3.361 ± 0.04
2.094LeuLys: 2.094 ± 0.036
11.331LeuLeu: 11.331 ± 0.121
1.681LeuMet: 1.681 ± 0.023
1.734LeuAsn: 1.734 ± 0.039
6.598LeuPro: 6.598 ± 0.065
2.089LeuGln: 2.089 ± 0.031
8.548LeuArg: 8.548 ± 0.081
5.286LeuSer: 5.286 ± 0.048
7.073LeuThr: 7.073 ± 0.063
8.815LeuVal: 8.815 ± 0.081
1.296LeuTrp: 1.296 ± 0.027
1.899LeuTyr: 1.899 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.215MetAla: 2.215 ± 0.032
0.121MetCys: 0.121 ± 0.008
0.912MetAsp: 0.912 ± 0.022
0.767MetGlu: 0.767 ± 0.019
0.462MetPhe: 0.462 ± 0.015
1.334MetGly: 1.334 ± 0.026
0.357MetHis: 0.357 ± 0.012
0.647MetIle: 0.647 ± 0.017
0.42MetLys: 0.42 ± 0.015
1.676MetLeu: 1.676 ± 0.029
0.306MetMet: 0.306 ± 0.013
0.452MetAsn: 0.452 ± 0.015
1.078MetPro: 1.078 ± 0.025
0.444MetGln: 0.444 ± 0.015
1.37MetArg: 1.37 ± 0.024
1.328MetSer: 1.328 ± 0.027
1.549MetThr: 1.549 ± 0.026
1.292MetVal: 1.292 ± 0.027
0.211MetTrp: 0.211 ± 0.011
0.342MetTyr: 0.342 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.244AsnAla: 2.244 ± 0.036
0.165AsnCys: 0.165 ± 0.008
1.049AsnAsp: 1.049 ± 0.027
0.851AsnGlu: 0.851 ± 0.021
0.49AsnPhe: 0.49 ± 0.016
2.04AsnGly: 2.04 ± 0.036
0.405AsnHis: 0.405 ± 0.013
0.67AsnIle: 0.67 ± 0.019
0.438AsnLys: 0.438 ± 0.016
1.643AsnLeu: 1.643 ± 0.028
0.311AsnMet: 0.311 ± 0.012
0.475AsnAsn: 0.475 ± 0.019
1.312AsnPro: 1.312 ± 0.031
0.524AsnGln: 0.524 ± 0.015
1.218AsnArg: 1.218 ± 0.026
0.909AsnSer: 0.909 ± 0.023
1.173AsnThr: 1.173 ± 0.025
1.37AsnVal: 1.37 ± 0.029
0.297AsnTrp: 0.297 ± 0.012
0.424AsnTyr: 0.424 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
9.137ProAla: 9.137 ± 0.087
0.299ProCys: 0.299 ± 0.013
4.645ProAsp: 4.645 ± 0.051
4.531ProGlu: 4.531 ± 0.052
1.541ProPhe: 1.541 ± 0.026
6.965ProGly: 6.965 ± 0.064
1.313ProHis: 1.313 ± 0.024
1.098ProIle: 1.098 ± 0.022
1.215ProLys: 1.215 ± 0.027
5.434ProLeu: 5.434 ± 0.056
0.966ProMet: 0.966 ± 0.026
0.856ProAsn: 0.856 ± 0.022
3.411ProPro: 3.411 ± 0.072
1.772ProGln: 1.772 ± 0.038
3.785ProArg: 3.785 ± 0.046
3.115ProSer: 3.115 ± 0.035
3.027ProThr: 3.027 ± 0.044
5.836ProVal: 5.836 ± 0.064
0.906ProTrp: 0.906 ± 0.021
1.441ProTyr: 1.441 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.484GlnAla: 3.484 ± 0.045
0.164GlnCys: 0.164 ± 0.009
1.402GlnAsp: 1.402 ± 0.026
1.446GlnGlu: 1.446 ± 0.025
0.658GlnPhe: 0.658 ± 0.016
2.234GlnGly: 2.234 ± 0.034
0.62GlnHis: 0.62 ± 0.019
1.027GlnIle: 1.027 ± 0.024
0.63GlnLys: 0.63 ± 0.019
2.983GlnLeu: 2.983 ± 0.042
0.469GlnMet: 0.469 ± 0.014
0.541GlnAsn: 0.541 ± 0.017
1.627GlnPro: 1.627 ± 0.039
1.252GlnGln: 1.252 ± 0.035
2.174GlnArg: 2.174 ± 0.033
1.237GlnSer: 1.237 ± 0.024
1.297GlnThr: 1.297 ± 0.026
2.257GlnVal: 2.257 ± 0.035
0.441GlnTrp: 0.441 ± 0.015
0.64GlnTyr: 0.64 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
9.853ArgAla: 9.853 ± 0.097
0.545ArgCys: 0.545 ± 0.017
4.197ArgAsp: 4.197 ± 0.05
4.635ArgGlu: 4.635 ± 0.05
2.284ArgPhe: 2.284 ± 0.034
5.797ArgGly: 5.797 ± 0.057
1.957ArgHis: 1.957 ± 0.031
3.247ArgIle: 3.247 ± 0.037
1.61ArgLys: 1.61 ± 0.028
8.627ArgLeu: 8.627 ± 0.077
1.692ArgMet: 1.692 ± 0.026
1.404ArgAsn: 1.404 ± 0.026
4.863ArgPro: 4.863 ± 0.069
2.07ArgGln: 2.07 ± 0.032
7.199ArgArg: 7.199 ± 0.081
3.896ArgSer: 3.896 ± 0.045
5.628ArgThr: 5.628 ± 0.064
5.799ArgVal: 5.799 ± 0.06
1.255ArgTrp: 1.255 ± 0.027
1.726ArgTyr: 1.726 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.72SerAla: 6.72 ± 0.061
0.411SerCys: 0.411 ± 0.015
2.695SerAsp: 2.695 ± 0.036
2.379SerGlu: 2.379 ± 0.039
1.573SerPhe: 1.573 ± 0.027
6.074SerGly: 6.074 ± 0.06
1.007SerHis: 1.007 ± 0.022
1.367SerIle: 1.367 ± 0.028
1.071SerLys: 1.071 ± 0.026
4.895SerLeu: 4.895 ± 0.051
1.057SerMet: 1.057 ± 0.024
0.879SerAsn: 0.879 ± 0.021
3.014SerPro: 3.014 ± 0.036
1.191SerGln: 1.191 ± 0.028
3.482SerArg: 3.482 ± 0.045
2.717SerSer: 2.717 ± 0.048
3.052SerThr: 3.052 ± 0.041
4.342SerVal: 4.342 ± 0.04
0.901SerTrp: 0.901 ± 0.023
1.265SerTyr: 1.265 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
9.244ThrAla: 9.244 ± 0.073
0.404ThrCys: 0.404 ± 0.013
3.805ThrAsp: 3.805 ± 0.043
3.333ThrGlu: 3.333 ± 0.041
1.645ThrPhe: 1.645 ± 0.026
7.255ThrGly: 7.255 ± 0.072
1.18ThrHis: 1.18 ± 0.023
1.646ThrIle: 1.646 ± 0.03
1.223ThrLys: 1.223 ± 0.027
5.865ThrLeu: 5.865 ± 0.052
0.896ThrMet: 0.896 ± 0.018
0.981ThrAsn: 0.981 ± 0.023
4.171ThrPro: 4.171 ± 0.051
1.269ThrGln: 1.269 ± 0.025
3.782ThrArg: 3.782 ± 0.039
3.138ThrSer: 3.138 ± 0.041
3.946ThrThr: 3.946 ± 0.06
6.187ThrVal: 6.187 ± 0.057
0.88ThrTrp: 0.88 ± 0.022
1.409ThrTyr: 1.409 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
10.629ValAla: 10.629 ± 0.085
0.746ValCys: 0.746 ± 0.018
4.797ValAsp: 4.797 ± 0.047
4.501ValGlu: 4.501 ± 0.051
2.455ValPhe: 2.455 ± 0.037
6.216ValGly: 6.216 ± 0.057
1.936ValHis: 1.936 ± 0.032
2.881ValIle: 2.881 ± 0.044
1.771ValLys: 1.771 ± 0.035
9.64ValLeu: 9.64 ± 0.089
1.438ValMet: 1.438 ± 0.029
1.677ValAsn: 1.677 ± 0.03
5.302ValPro: 5.302 ± 0.053
2.04ValGln: 2.04 ± 0.028
7.259ValArg: 7.259 ± 0.073
4.352ValSer: 4.352 ± 0.045
5.803ValThr: 5.803 ± 0.056
7.813ValVal: 7.813 ± 0.075
1.132ValTrp: 1.132 ± 0.022
1.573ValTyr: 1.573 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.646TrpAla: 1.646 ± 0.031
0.147TrpCys: 0.147 ± 0.008
0.828TrpAsp: 0.828 ± 0.019
0.718TrpGlu: 0.718 ± 0.019
0.517TrpPhe: 0.517 ± 0.015
1.045TrpGly: 1.045 ± 0.022
0.353TrpHis: 0.353 ± 0.012
0.549TrpIle: 0.549 ± 0.016
0.369TrpLys: 0.369 ± 0.015
1.682TrpLeu: 1.682 ± 0.026
0.293TrpMet: 0.293 ± 0.012
0.409TrpAsn: 0.409 ± 0.013
0.78TrpPro: 0.78 ± 0.018
0.629TrpGln: 0.629 ± 0.016
1.258TrpArg: 1.258 ± 0.029
0.928TrpSer: 0.928 ± 0.022
1.092TrpThr: 1.092 ± 0.024
0.983TrpVal: 0.983 ± 0.023
0.32TrpTrp: 0.32 ± 0.014
0.365TrpTyr: 0.365 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.946TyrAla: 2.946 ± 0.037
0.166TyrCys: 0.166 ± 0.01
1.494TyrAsp: 1.494 ± 0.032
1.295TyrGlu: 1.295 ± 0.026
0.661TyrPhe: 0.661 ± 0.02
2.353TyrGly: 2.353 ± 0.036
0.373TyrHis: 0.373 ± 0.013
0.503TyrIle: 0.503 ± 0.015
0.425TyrLys: 0.425 ± 0.017
2.094TyrLeu: 2.094 ± 0.032
0.265TyrMet: 0.265 ± 0.012
0.454TyrAsn: 0.454 ± 0.016
1.104TyrPro: 1.104 ± 0.024
0.588TyrGln: 0.588 ± 0.016
1.816TyrArg: 1.816 ± 0.03
0.99TyrSer: 0.99 ± 0.025
1.321TyrThr: 1.321 ± 0.024
1.705TyrVal: 1.705 ± 0.03
0.373TyrTrp: 0.373 ± 0.013
0.476TyrTyr: 0.476 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6535 proteins (2242174 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski