Broad bean necrosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Pomovirus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9YPH2|Q9YPH2_9VIRU Triple-gene-block second protein OS=Broad bean necrosis virus OX=79918 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 5.53PPVIINSRR10 pKa = 11.84DD11 pKa = 3.72CSCQHH16 pKa = 6.74CYY18 pKa = 10.44LPQRR22 pKa = 11.84CSHH25 pKa = 6.38SSGSVSQDD33 pKa = 2.86VQSPVEE39 pKa = 4.34EE40 pKa = 3.91IVQIVSNRR48 pKa = 11.84EE49 pKa = 3.98SVFDD53 pKa = 3.73SYY55 pKa = 12.1YY56 pKa = 11.45LLICCSVCFILGLSIMLFINNLYY79 pKa = 9.38FRR81 pKa = 11.84NVSSVGGSYY90 pKa = 10.73YY91 pKa = 10.74YY92 pKa = 10.57QDD94 pKa = 4.28LNSVEE99 pKa = 4.45YY100 pKa = 10.34KK101 pKa = 10.56SSGPIDD107 pKa = 3.87ADD109 pKa = 3.6VIEE112 pKa = 5.62RR113 pKa = 11.84IHH115 pKa = 6.78HH116 pKa = 5.78FQQGPLGRR124 pKa = 11.84FKK126 pKa = 11.26DD127 pKa = 3.76DD128 pKa = 2.76ATFAIKK134 pKa = 10.53VKK136 pKa = 10.51EE137 pKa = 3.9DD138 pKa = 3.92DD139 pKa = 3.99FFEE142 pKa = 4.66DD143 pKa = 3.67AVDD146 pKa = 3.36IRR148 pKa = 11.84EE149 pKa = 4.03TSLFISDD156 pKa = 3.85KK157 pKa = 9.76VVSFVFIIILILLLKK172 pKa = 9.86VCYY175 pKa = 10.1GG176 pKa = 3.41

Molecular weight: 19.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9YPH3|Q9YPH3_9VIRU Triple-gene-block first protein (Fragment) OS=Broad bean necrosis virus OX=79918 PE=4 SV=1
MM1 pKa = 6.98VRR3 pKa = 11.84SNEE6 pKa = 3.76IGSRR10 pKa = 11.84PNKK13 pKa = 9.18YY14 pKa = 8.8WPILFGVSAICFFLFLGVTNQNIPHH39 pKa = 6.25NHH41 pKa = 6.59HH42 pKa = 7.07GDD44 pKa = 3.95NIHH47 pKa = 6.59KK48 pKa = 9.97FSNGGKK54 pKa = 9.09YY55 pKa = 9.21QDD57 pKa = 3.17GTKK60 pKa = 10.25RR61 pKa = 11.84INYY64 pKa = 7.3NANNSRR70 pKa = 11.84AYY72 pKa = 10.54NGSSSNNQFKK82 pKa = 11.09GLFLPALLFTAAMLAFQWFSKK103 pKa = 10.11SRR105 pKa = 11.84CPVTCRR111 pKa = 11.84GDD113 pKa = 3.63CANCQQ118 pKa = 3.21

Molecular weight: 13.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4742

60

1821

592.8

67.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.947 ± 0.505

2.13 ± 0.34

6.369 ± 0.692

7.465 ± 0.87

4.407 ± 0.328

5.272 ± 0.586

1.792 ± 0.219

6.284 ± 0.585

7.381 ± 0.603

8.962 ± 0.681

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.889 ± 0.47

5.293 ± 0.441

3.016 ± 0.225

3.48 ± 0.209

5.293 ± 0.267

7.339 ± 0.502

5.082 ± 0.318

6.664 ± 0.298

1.202 ± 0.13

3.69 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski