Solimicrobium silvestre
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4741 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S9GZW5|A0A2S9GZW5_9BURK Ribosomal RNA small subunit methyltransferase I OS=Solimicrobium silvestre OX=2099400 GN=rsmI PE=3 SV=1
MM1 pKa = 6.94 NTRR4 pKa = 11.84 NQTFVKK10 pKa = 10.05 KK11 pKa = 10.56 ALFAMMFVGASAQAVPFTYY30 pKa = 9.7 TGSIVDD36 pKa = 3.66 YY37 pKa = 9.61 TITTSGVYY45 pKa = 10.54 DD46 pKa = 3.83 LTAAGAQGGSGNGVGTTGGLGAQISGDD73 pKa = 3.58 LFLTTGTQLGIVVGGQGLTGNFGGLAGGGGGGGSFVYY110 pKa = 10.21 IIGSTSPLIVAGGGGGAGYY129 pKa = 10.09 IDD131 pKa = 3.76 ATVGGGGQTTTSGQAGFGAGGGAGGSAGSGGAGGNDD167 pKa = 3.26 EE168 pKa = 5.1 IGDD171 pKa = 3.77 NGGGGGGWGGNGGDD185 pKa = 4.16 GVGTTYY191 pKa = 9.93 TQTGGAGLGGAGAFSFLAGFGDD213 pKa = 4.68 CDD215 pKa = 3.73 PSVPACANGGFGGGGGGGWQGGGGGGGYY243 pKa = 9.95 SGGGGGDD250 pKa = 3.4 GTTDD254 pKa = 3.06 GGGGGGSYY262 pKa = 9.94 IDD264 pKa = 4.77 ASFLSIGSMIGGSDD278 pKa = 3.2 TSDD281 pKa = 2.94 NGAPLGNANNGFVNIDD297 pKa = 3.53 MVLLSNPVPEE307 pKa = 4.88 PLTLALMAIGLAGLGFIRR325 pKa = 11.84 RR326 pKa = 11.84 KK327 pKa = 8.83 KK328 pKa = 10.59 ARR330 pKa = 11.84 II331 pKa = 3.45
Molecular weight: 30.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.897
IPC_protein 3.884
Toseland 3.643
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.91
Rodwell 3.706
Grimsley 3.554
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.355
Thurlkill 3.732
EMBOSS 3.91
Sillero 4.012
Patrickios 0.947
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|A0A2S9H2A8|A0A2S9H2A8_9BURK PPIC-type PPIASE domain OS=Solimicrobium silvestre OX=2099400 GN=S2091_1271 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.57 RR14 pKa = 11.84 THH16 pKa = 5.79 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4741
0
4741
1520618
29
15885
320.7
35.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.218 ± 0.045
0.966 ± 0.016
5.033 ± 0.024
5.177 ± 0.056
3.972 ± 0.033
7.132 ± 0.053
2.252 ± 0.031
6.244 ± 0.036
4.527 ± 0.055
10.378 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.565 ± 0.027
4.272 ± 0.056
4.419 ± 0.022
4.495 ± 0.038
4.844 ± 0.062
6.684 ± 0.06
5.876 ± 0.098
6.892 ± 0.034
1.281 ± 0.014
2.774 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here