Jannaschia seohaensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Jannaschia

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Y9B033|A0A2Y9B033_9RHOB Uncharacterized protein OS=Jannaschia seohaensis OX=475081 GN=BCF38_10896 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.32KK3 pKa = 9.94LASSTAIAVLLSSAAYY19 pKa = 10.34ADD21 pKa = 3.52GHH23 pKa = 5.67TAAFTPGEE31 pKa = 4.59GPFDD35 pKa = 3.63WDD37 pKa = 3.57SYY39 pKa = 10.48NAFAEE44 pKa = 4.37ATDD47 pKa = 3.96LTGQTVTVTGPWTGDD62 pKa = 2.85EE63 pKa = 4.06AAKK66 pKa = 10.35FDD68 pKa = 3.45IVMAFFADD76 pKa = 3.41ATGAEE81 pKa = 4.46VNYY84 pKa = 10.53SGSDD88 pKa = 3.18SFEE91 pKa = 3.7QDD93 pKa = 2.49IVISSRR99 pKa = 11.84AGSAPNLAVFPQPGLAADD117 pKa = 3.95MASQGLLTPLPDD129 pKa = 3.7GTADD133 pKa = 3.04WVAEE137 pKa = 4.0NFAAGQSWVDD147 pKa = 3.33LGTYY151 pKa = 10.06AGEE154 pKa = 4.55DD155 pKa = 4.12GEE157 pKa = 5.35DD158 pKa = 3.42DD159 pKa = 4.38LYY161 pKa = 11.88GMFYY165 pKa = 10.51RR166 pKa = 11.84VDD168 pKa = 3.71LKK170 pKa = 11.43SLVWYY175 pKa = 10.44SPAAFDD181 pKa = 4.69EE182 pKa = 4.24MGYY185 pKa = 10.38DD186 pKa = 2.95IPTTMEE192 pKa = 3.96EE193 pKa = 4.35LKK195 pKa = 10.74EE196 pKa = 3.93LTDD199 pKa = 3.67QIVADD204 pKa = 5.74GEE206 pKa = 4.8TPWCIGLGSGAATGWPATDD225 pKa = 3.12WVEE228 pKa = 5.29DD229 pKa = 3.55MMLRR233 pKa = 11.84TQPPSVYY240 pKa = 10.17DD241 pKa = 3.08AWVSNEE247 pKa = 4.22LPFDD251 pKa = 3.96APEE254 pKa = 3.85VVAAIEE260 pKa = 4.05EE261 pKa = 4.19FGYY264 pKa = 10.0FARR267 pKa = 11.84NDD269 pKa = 3.81DD270 pKa = 4.09YY271 pKa = 12.1VSGGSQAVASTDD283 pKa = 3.98FRR285 pKa = 11.84DD286 pKa = 3.68SPTGLFAIPPACYY299 pKa = 8.65MHH301 pKa = 6.78RR302 pKa = 11.84QASFIPAFFPEE313 pKa = 4.61GTVVGEE319 pKa = 4.55DD320 pKa = 2.7VDD322 pKa = 5.26FFYY325 pKa = 11.13FPAYY329 pKa = 10.05ADD331 pKa = 3.98KK332 pKa = 11.33DD333 pKa = 3.54LGAPVLGAGTLFAITNPSEE352 pKa = 3.87GAEE355 pKa = 3.92AMIEE359 pKa = 4.06FLKK362 pKa = 11.07LPIAHH367 pKa = 6.73EE368 pKa = 3.96LWMAQGGFLTAHH380 pKa = 6.9AGVNTDD386 pKa = 4.15AYY388 pKa = 11.27SEE390 pKa = 4.36DD391 pKa = 3.63SLRR394 pKa = 11.84AQGQILLDD402 pKa = 3.38ATTFRR407 pKa = 11.84FDD409 pKa = 5.22ASDD412 pKa = 3.81LMPGEE417 pKa = 4.37IGAGAFWTGMVDD429 pKa = 3.67YY430 pKa = 9.84VTGASAEE437 pKa = 4.09EE438 pKa = 4.18VAAGVQEE445 pKa = 4.55RR446 pKa = 11.84WQSLNN451 pKa = 3.22

Molecular weight: 48.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Y9AB67|A0A2Y9AB67_9RHOB Uncharacterized protein OS=Jannaschia seohaensis OX=475081 GN=BCF38_102252 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4631

0

4631

1425063

28

10108

307.7

33.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.525 ± 0.064

0.842 ± 0.012

6.208 ± 0.049

6.019 ± 0.034

3.464 ± 0.024

9.173 ± 0.054

1.959 ± 0.018

4.643 ± 0.03

2.267 ± 0.031

10.35 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.024

2.054 ± 0.024

5.593 ± 0.034

2.747 ± 0.018

7.556 ± 0.052

4.739 ± 0.027

5.513 ± 0.033

7.379 ± 0.029

1.477 ± 0.021

1.948 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski