Rhizoctonia solani dsRNA virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1BPE2|A0A2D1BPE2_9VIRU RNA-dependent RNA polymerase OS=Rhizoctonia solani dsRNA virus 4 OX=2045504 PE=4 SV=1
MM1 pKa = 7.57TDD3 pKa = 4.33KK4 pKa = 10.7ISQSTVGAIAAEE16 pKa = 4.27PSPPAGPAPATRR28 pKa = 11.84AEE30 pKa = 4.61PNPPPAPTATSKK42 pKa = 10.96PKK44 pKa = 10.73GKK46 pKa = 9.86LRR48 pKa = 11.84PASNFTPTSHH58 pKa = 7.06NGIAPMLQVVSNRR71 pKa = 11.84RR72 pKa = 11.84SHH74 pKa = 6.97IINRR78 pKa = 11.84AEE80 pKa = 3.86PSGLFPSAINYY91 pKa = 7.15FQAVSITDD99 pKa = 3.26QRR101 pKa = 11.84MSTTKK106 pKa = 10.51KK107 pKa = 10.04FLEE110 pKa = 4.74TSEE113 pKa = 4.04SWHH116 pKa = 6.21PVVSQYY122 pKa = 9.66YY123 pKa = 8.6ACFLFTVQVLRR134 pKa = 11.84TYY136 pKa = 10.41NVSKK140 pKa = 10.87IPDD143 pKa = 3.72TEE145 pKa = 4.14GLDD148 pKa = 3.31FLSYY152 pKa = 11.04VEE154 pKa = 4.41EE155 pKa = 4.17QLDD158 pKa = 3.81LTQIPVPGTWAEE170 pKa = 3.86YY171 pKa = 10.25LRR173 pKa = 11.84NITVVEE179 pKa = 3.93AHH181 pKa = 6.88HH182 pKa = 7.2DD183 pKa = 3.72SFGNIHH189 pKa = 6.65PVFPLLGGSPLTAGDD204 pKa = 3.75VFSFPQSHH212 pKa = 6.29RR213 pKa = 11.84RR214 pKa = 11.84ILPNCYY220 pKa = 9.57LALDD224 pKa = 3.54QLIRR228 pKa = 11.84YY229 pKa = 7.25AQTGANTNSPRR240 pKa = 11.84FINYY244 pKa = 10.12ANIFQLNPTTSDD256 pKa = 2.79EE257 pKa = 4.06AAQAKK262 pKa = 8.31LAPQVSSWSHH272 pKa = 4.42VSNSRR277 pKa = 11.84NDD279 pKa = 3.08VSLEE283 pKa = 3.72FWANAATLLPDD294 pKa = 3.91RR295 pKa = 11.84ADD297 pKa = 3.25SRR299 pKa = 11.84TGHH302 pKa = 6.64HH303 pKa = 6.65SGDD306 pKa = 3.44VTDD309 pKa = 5.8FFVFTGFRR317 pKa = 11.84NAAGTASHH325 pKa = 6.66SFFDD329 pKa = 5.51VILQDD334 pKa = 3.34MSSYY338 pKa = 10.18CQFVKK343 pKa = 10.91GSVPLSTIQSIGIGATIPVMYY364 pKa = 9.03VQPSTSVRR372 pKa = 11.84NYY374 pKa = 8.8FFPSLADD381 pKa = 3.78LRR383 pKa = 11.84DD384 pKa = 3.31NTNYY388 pKa = 9.57HH389 pKa = 6.55RR390 pKa = 11.84SGHH393 pKa = 5.87RR394 pKa = 11.84LFPTTITYY402 pKa = 11.01DD403 pKa = 3.39MRR405 pKa = 11.84HH406 pKa = 5.93GDD408 pKa = 3.48LTLEE412 pKa = 4.68EE413 pKa = 4.4IAEE416 pKa = 4.23QYY418 pKa = 11.57AKK420 pKa = 9.42LTCLNTSFRR429 pKa = 11.84DD430 pKa = 3.58LPTQNGWNQPDD441 pKa = 3.73EE442 pKa = 4.5TTVRR446 pKa = 11.84DD447 pKa = 4.11GPFWAITEE455 pKa = 4.27YY456 pKa = 11.09KK457 pKa = 9.7VDD459 pKa = 3.64RR460 pKa = 11.84GVNPITGLLTNIPAYY475 pKa = 9.49YY476 pKa = 9.94HH477 pKa = 6.37SPNAVANKK485 pKa = 10.01

Molecular weight: 53.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1BPE2|A0A2D1BPE2_9VIRU RNA-dependent RNA polymerase OS=Rhizoctonia solani dsRNA virus 4 OX=2045504 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.22YY3 pKa = 10.23INRR6 pKa = 11.84VLSYY10 pKa = 9.89IFKK13 pKa = 10.56PKK15 pKa = 10.01FEE17 pKa = 4.6EE18 pKa = 4.29YY19 pKa = 11.16NFIEE23 pKa = 4.12VGRR26 pKa = 11.84YY27 pKa = 8.25SRR29 pKa = 11.84PPHH32 pKa = 6.91VPTQDD37 pKa = 3.05TLSIEE42 pKa = 3.91KK43 pKa = 9.33HH44 pKa = 5.32QNVMMHH50 pKa = 6.05SMRR53 pKa = 11.84KK54 pKa = 9.05YY55 pKa = 9.07LYY57 pKa = 8.49PWEE60 pKa = 4.34IEE62 pKa = 3.97QIEE65 pKa = 4.88SYY67 pKa = 10.84RR68 pKa = 11.84RR69 pKa = 11.84TTTSEE74 pKa = 3.55SDD76 pKa = 2.95IEE78 pKa = 4.39RR79 pKa = 11.84DD80 pKa = 3.46FFTGNVEE87 pKa = 4.58HH88 pKa = 7.33FDD90 pKa = 3.95PPLDD94 pKa = 3.82EE95 pKa = 4.78NFEE98 pKa = 4.59LGLADD103 pKa = 3.68MLDD106 pKa = 3.75AFRR109 pKa = 11.84PPQKK113 pKa = 10.22CRR115 pKa = 11.84PCHH118 pKa = 5.9INDD121 pKa = 3.87IEE123 pKa = 4.02HH124 pKa = 7.15HH125 pKa = 6.91YY126 pKa = 9.06PFKK129 pKa = 10.09WQVNAEE135 pKa = 4.38APFSTDD141 pKa = 5.66DD142 pKa = 3.52YY143 pKa = 11.61FLDD146 pKa = 3.73NRR148 pKa = 11.84PKK150 pKa = 10.2ISEE153 pKa = 4.21LADD156 pKa = 3.28SKK158 pKa = 11.52YY159 pKa = 11.21AFLDD163 pKa = 3.04QRR165 pKa = 11.84YY166 pKa = 9.19VDD168 pKa = 4.44RR169 pKa = 11.84LLSKK173 pKa = 9.31QTSEE177 pKa = 4.23MNNPVPAKK185 pKa = 9.6FGPMRR190 pKa = 11.84HH191 pKa = 5.46TVFNWTRR198 pKa = 11.84RR199 pKa = 11.84WHH201 pKa = 6.44HH202 pKa = 5.72VIKK205 pKa = 10.89DD206 pKa = 3.34GFEE209 pKa = 3.86RR210 pKa = 11.84HH211 pKa = 5.95AHH213 pKa = 6.02LKK215 pKa = 11.12DD216 pKa = 3.29NDD218 pKa = 4.02PYY220 pKa = 10.96FQNKK224 pKa = 9.72YY225 pKa = 8.89IFPMLLHH232 pKa = 7.04AKK234 pKa = 8.27TAIVKK239 pKa = 10.28KK240 pKa = 10.44DD241 pKa = 3.95DD242 pKa = 4.07PNKK245 pKa = 9.8MRR247 pKa = 11.84TIWGCSKK254 pKa = 10.23PWIIADD260 pKa = 3.27AMLYY264 pKa = 9.31WEE266 pKa = 4.52YY267 pKa = 10.78IAWIKK272 pKa = 10.3LNPGLTPMLWGYY284 pKa = 7.79EE285 pKa = 4.05TMTGGWMRR293 pKa = 11.84LNAALFSSYY302 pKa = 11.1LNKK305 pKa = 10.48CYY307 pKa = 9.66LTLDD311 pKa = 3.49WSRR314 pKa = 11.84FDD316 pKa = 3.04KK317 pKa = 10.71RR318 pKa = 11.84AYY320 pKa = 9.91FSIIRR325 pKa = 11.84RR326 pKa = 11.84IMFGILTYY334 pKa = 10.95LDD336 pKa = 3.84FDD338 pKa = 3.65NGYY341 pKa = 9.48VPNVNYY347 pKa = 9.95PDD349 pKa = 3.94TKK351 pKa = 11.01SDD353 pKa = 3.09WSHH356 pKa = 6.63LKK358 pKa = 9.46SQRR361 pKa = 11.84ILRR364 pKa = 11.84LWLWTLDD371 pKa = 3.69NLFNAPIVLPDD382 pKa = 2.78GRR384 pKa = 11.84MYY386 pKa = 11.0KK387 pKa = 10.2RR388 pKa = 11.84LFAGIPSGLFITQLLDD404 pKa = 2.7SWYY407 pKa = 9.6NYY409 pKa = 9.02TMIATILRR417 pKa = 11.84AMGFNPRR424 pKa = 11.84QCIIKK429 pKa = 9.98VQGDD433 pKa = 3.6DD434 pKa = 3.9SIVRR438 pKa = 11.84LYY440 pKa = 11.33VLIPPAKK447 pKa = 10.39HH448 pKa = 6.2EE449 pKa = 4.37DD450 pKa = 3.29FLLRR454 pKa = 11.84MQEE457 pKa = 4.0LADD460 pKa = 4.14HH461 pKa = 6.04YY462 pKa = 9.39FKK464 pKa = 11.25SVISVDD470 pKa = 3.1KK471 pKa = 11.48SEE473 pKa = 5.01IGSSLNGRR481 pKa = 11.84EE482 pKa = 3.88VLSYY486 pKa = 10.61RR487 pKa = 11.84NKK489 pKa = 10.43NGFPFRR495 pKa = 11.84DD496 pKa = 4.15EE497 pKa = 4.08IKK499 pKa = 10.24MIAQFYY505 pKa = 7.08HH506 pKa = 5.15TKK508 pKa = 10.25ARR510 pKa = 11.84KK511 pKa = 7.28STPEE515 pKa = 3.28IAMAQAIGFAYY526 pKa = 10.15ASCGNHH532 pKa = 6.52DD533 pKa = 3.93RR534 pKa = 11.84VLSVLEE540 pKa = 4.37DD541 pKa = 3.54MYY543 pKa = 11.84DD544 pKa = 3.2HH545 pKa = 6.78YY546 pKa = 11.29LSQGYY551 pKa = 9.3SPNPAGLTMVFGNSPDD567 pKa = 3.64TEE569 pKa = 4.2RR570 pKa = 11.84PFIDD574 pKa = 3.4MDD576 pKa = 3.85HH577 pKa = 6.77FPSISEE583 pKa = 3.6IKK585 pKa = 10.34RR586 pKa = 11.84FLVCTDD592 pKa = 3.1YY593 pKa = 11.58RR594 pKa = 11.84NDD596 pKa = 3.39VQSRR600 pKa = 11.84RR601 pKa = 11.84TWPPDD606 pKa = 3.43YY607 pKa = 10.28FLHH610 pKa = 7.08PPACKK615 pKa = 10.12PEE617 pKa = 3.73

Molecular weight: 72.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1102

485

617

551.0

63.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.534 ± 1.195

1.089 ± 0.164

6.171 ± 0.761

4.265 ± 0.473

5.717 ± 0.222

4.537 ± 0.384

3.267 ± 0.108

5.989 ± 0.519

4.174 ± 1.058

7.804 ± 0.366

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.868

5.445 ± 0.333

7.441 ± 0.502

3.448 ± 0.549

5.808 ± 0.535

7.441 ± 1.015

6.443 ± 1.508

4.991 ± 0.743

1.996 ± 0.473

4.809 ± 0.683
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski