Streptococcus phage Javan290
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AZV3|A0A4D6AZV3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan290 OX=2548096 GN=Javan290_0042 PE=4 SV=1
MM1 pKa = 7.28 NWNSLHH7 pKa = 7.27 DD8 pKa = 4.03 FLGEE12 pKa = 4.04 NIKK15 pKa = 8.88 VTFIDD20 pKa = 3.72 DD21 pKa = 3.85 TVLEE25 pKa = 5.53 GYY27 pKa = 10.73 CNTYY31 pKa = 8.94 TGKK34 pKa = 10.67 LDD36 pKa = 3.87 TEE38 pKa = 4.29 EE39 pKa = 4.24 EE40 pKa = 4.66 LYY42 pKa = 11.18 DD43 pKa = 4.35 EE44 pKa = 4.51 ITIKK48 pKa = 9.44 TAQHH52 pKa = 5.7 QYY54 pKa = 10.43 VGFNEE59 pKa = 4.33 SEE61 pKa = 3.99 IKK63 pKa = 10.7 SIEE66 pKa = 4.08 LVV68 pKa = 3.34
Molecular weight: 7.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.924
IPC2_protein 4.228
IPC_protein 4.088
Toseland 3.935
ProMoST 4.215
Dawson 4.037
Bjellqvist 4.202
Wikipedia 3.923
Rodwell 3.935
Grimsley 3.846
Solomon 4.024
Lehninger 3.986
Nozaki 4.164
DTASelect 4.279
Thurlkill 3.961
EMBOSS 3.935
Sillero 4.202
Patrickios 1.939
IPC_peptide 4.037
IPC2_peptide 4.19
IPC2.peptide.svr19 4.098
Protein with the highest isoelectric point:
>tr|A0A4D6B266|A0A4D6B266_9CAUD Uncharacterized protein OS=Streptococcus phage Javan290 OX=2548096 GN=Javan290_0058 PE=4 SV=1
MM1 pKa = 7.8 AKK3 pKa = 8.42 NTKK6 pKa = 7.57 QTSAKK11 pKa = 9.64 VARR14 pKa = 11.84 QASKK18 pKa = 10.86 ALRR21 pKa = 11.84 DD22 pKa = 3.82 GRR24 pKa = 11.84 SSARR28 pKa = 11.84 TKK30 pKa = 10.73 SIAGSALSQAKK41 pKa = 9.93 GKK43 pKa = 9.92 SSKK46 pKa = 10.49 RR47 pKa = 11.84 KK48 pKa = 9.17
Molecular weight: 5.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.244
IPC2_protein 10.555
IPC_protein 12.018
Toseland 12.237
ProMoST 12.676
Dawson 12.237
Bjellqvist 12.193
Wikipedia 12.676
Rodwell 12.281
Grimsley 12.266
Solomon 12.691
Lehninger 12.603
Nozaki 12.223
DTASelect 12.193
Thurlkill 12.223
EMBOSS 12.72
Sillero 12.223
Patrickios 12.018
IPC_peptide 12.705
IPC2_peptide 11.667
IPC2.peptide.svr19 8.983
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
10479
38
778
163.7
18.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.1 ± 0.486
0.553 ± 0.099
6.06 ± 0.282
8.006 ± 0.515
3.846 ± 0.194
7.224 ± 0.495
1.622 ± 0.138
6.346 ± 0.263
7.768 ± 0.467
8.465 ± 0.285
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.168
4.695 ± 0.286
2.72 ± 0.184
4.18 ± 0.161
4.686 ± 0.342
6.422 ± 0.357
5.831 ± 0.266
7.062 ± 0.344
1.241 ± 0.112
3.645 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here