Aliikangiella marina
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4367 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A545T2U9|A0A545T2U9_9GAMM Maleylacetoacetate isomerase OS=Aliikangiella marina OX=1712262 GN=maiA PE=3 SV=1
MM1 pKa = 7.59 INLKK5 pKa = 10.47 KK6 pKa = 10.64 SLLAGAIAACSLSAAAVEE24 pKa = 4.1 TAYY27 pKa = 11.14 NIVPLGTFEE36 pKa = 5.12 DD37 pKa = 3.99 NEE39 pKa = 3.96 IFAFGINNNDD49 pKa = 3.1 VAVGYY54 pKa = 10.44 SYY56 pKa = 10.49 VTGGFPSRR64 pKa = 11.84 GYY66 pKa = 11.03 AYY68 pKa = 10.58 DD69 pKa = 3.88 NGTFTNMGSIVNQEE83 pKa = 3.83 LAIGFIAATIDD94 pKa = 3.24 EE95 pKa = 4.76 SVAFDD100 pKa = 3.57 INDD103 pKa = 3.34 NGMMIGYY110 pKa = 9.0 SVEE113 pKa = 4.2 SVPQFDD119 pKa = 4.53 EE120 pKa = 4.65 NNDD123 pKa = 3.75 PVTVTVDD130 pKa = 3.03 QGLDD134 pKa = 3.41 TEE136 pKa = 5.33 RR137 pKa = 11.84 EE138 pKa = 4.26 LQSGLPVQRR147 pKa = 11.84 AIYY150 pKa = 8.01 TQVGSQVLSQVPLFEE165 pKa = 5.07 PEE167 pKa = 3.38 QDD169 pKa = 3.1 HH170 pKa = 6.26 GVRR173 pKa = 11.84 ALAVNNNGVIVGFAEE188 pKa = 5.04 LDD190 pKa = 3.46 VDD192 pKa = 5.49 DD193 pKa = 6.0 DD194 pKa = 4.08 LTAEE198 pKa = 4.45 GEE200 pKa = 4.08 DD201 pKa = 3.79 AEE203 pKa = 5.03 FIVNRR208 pKa = 11.84 GVIYY212 pKa = 8.03 DD213 pKa = 3.99 TQTDD217 pKa = 3.54 TLTRR221 pKa = 11.84 VNPLNYY227 pKa = 10.25 DD228 pKa = 3.49 NLPRR232 pKa = 11.84 SIALKK237 pKa = 10.27 DD238 pKa = 3.37 INDD241 pKa = 3.57 NGVAVGWSQVAFEE254 pKa = 5.07 SSFLFNGVVVNAASPEE270 pKa = 4.18 TLTTIDD276 pKa = 4.2 PGEE279 pKa = 4.12 EE280 pKa = 3.7 TASLFNAINNNGMVAGKK297 pKa = 10.45 LYY299 pKa = 10.77 DD300 pKa = 3.76 LEE302 pKa = 4.15 KK303 pKa = 10.53 QRR305 pKa = 11.84 YY306 pKa = 7.19 EE307 pKa = 4.15 GIMFNSNNGAFEE319 pKa = 4.91 IIPPLVEE326 pKa = 4.79 GLDD329 pKa = 3.96 SQICCDD335 pKa = 3.47 NASPYY340 pKa = 10.49 DD341 pKa = 3.67 INAQNIIVGSMIADD355 pKa = 3.78 VVPDD359 pKa = 4.1 TYY361 pKa = 11.16 HH362 pKa = 7.16 AFAYY366 pKa = 10.45 QNGTTYY372 pKa = 11.34 DD373 pKa = 3.92 LNDD376 pKa = 5.88 LIDD379 pKa = 4.07 CTQTLTTGVRR389 pKa = 11.84 DD390 pKa = 3.41 WVLYY394 pKa = 7.6 EE395 pKa = 4.07 ARR397 pKa = 11.84 QINDD401 pKa = 2.89 NGIIIGNGLFGGEE414 pKa = 3.84 RR415 pKa = 11.84 KK416 pKa = 10.33 AFMLTPNPGATPTQCDD432 pKa = 3.92 FEE434 pKa = 4.61 QEE436 pKa = 4.35 EE437 pKa = 4.52 EE438 pKa = 4.28 DD439 pKa = 4.19 SGSGSIPASLIVLLSCLAVIRR460 pKa = 11.84 RR461 pKa = 11.84 KK462 pKa = 10.41 VSS464 pKa = 2.84
Molecular weight: 49.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 1.329
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A545T2F4|A0A545T2F4_9GAMM Branched-chain alpha-keto acid dehydrogenase subunit E2 OS=Aliikangiella marina OX=1712262 GN=FLL45_19770 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSTLKK11 pKa = 10.3 RR12 pKa = 11.84 KK13 pKa = 9.12 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.47 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.45 NGRR28 pKa = 11.84 QVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.7 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4367
0
4367
1527963
37
8991
349.9
39.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.959 ± 0.049
0.93 ± 0.012
5.699 ± 0.03
6.593 ± 0.036
4.464 ± 0.026
6.411 ± 0.037
1.987 ± 0.016
6.808 ± 0.03
5.853 ± 0.038
10.073 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.256 ± 0.019
4.866 ± 0.03
3.798 ± 0.022
4.504 ± 0.027
4.502 ± 0.025
7.139 ± 0.034
5.172 ± 0.029
6.629 ± 0.028
1.244 ± 0.013
3.112 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here