Escherichia phage T7 (Bacteriophage T7)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus; Escherichia virus T7

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03776|GP04_BPT7 Gene 0.4 protein OS=Escherichia phage T7 OX=10760 PE=1 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 11.18NNVFDD13 pKa = 4.72HH14 pKa = 6.93AYY16 pKa = 10.49EE17 pKa = 3.86MLKK20 pKa = 10.85EE21 pKa = 4.08NIRR24 pKa = 11.84YY25 pKa = 9.81DD26 pKa = 4.67DD27 pKa = 4.5IRR29 pKa = 11.84DD30 pKa = 3.55TDD32 pKa = 4.04DD33 pKa = 3.31LHH35 pKa = 7.82DD36 pKa = 5.82AIHH39 pKa = 6.1MAADD43 pKa = 3.49NAVPHH48 pKa = 6.35YY49 pKa = 9.7YY50 pKa = 11.01ADD52 pKa = 3.56IFSVMASEE60 pKa = 5.55GIDD63 pKa = 4.5LEE65 pKa = 4.76FEE67 pKa = 4.73DD68 pKa = 5.02SGLMPDD74 pKa = 3.4TKK76 pKa = 10.94DD77 pKa = 3.64VIRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 9.25EE88 pKa = 4.14QLTIDD93 pKa = 3.64LWEE96 pKa = 4.69DD97 pKa = 3.57AEE99 pKa = 4.86DD100 pKa = 4.13LLNEE104 pKa = 3.98YY105 pKa = 10.56LEE107 pKa = 4.27EE108 pKa = 4.17VEE110 pKa = 5.35EE111 pKa = 4.33YY112 pKa = 10.97EE113 pKa = 4.56EE114 pKa = 5.54DD115 pKa = 3.76EE116 pKa = 4.41EE117 pKa = 4.69

Molecular weight: 13.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03783|Y42_BPT7 Preotein 4.2 OS=Escherichia phage T7 OX=10760 GN=4.2 PE=4 SV=1
MM1 pKa = 7.35GTRR4 pKa = 11.84CSLTDD9 pKa = 3.41TRR11 pKa = 11.84SATYY15 pKa = 10.23ARR17 pKa = 11.84SGLLVMKK24 pKa = 8.39TLKK27 pKa = 10.52RR28 pKa = 11.84GLQNGNPQEE37 pKa = 4.28VNQQ40 pKa = 4.07

Molecular weight: 4.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

2

59

13690

29

1318

232.0

25.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.941 ± 0.482

1.242 ± 0.167

6.26 ± 0.248

7.173 ± 0.312

3.521 ± 0.172

7.414 ± 0.31

1.994 ± 0.17

5.274 ± 0.177

6.749 ± 0.287

8.167 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.119 ± 0.17

4.522 ± 0.267

3.375 ± 0.195

3.682 ± 0.31

5.698 ± 0.231

6.063 ± 0.282

5.617 ± 0.202

6.399 ± 0.251

1.556 ± 0.167

3.236 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski