Amino acid dipepetide frequency for Vibrio phage VP585

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.633AlaAla: 11.633 ± 1.678
0.912AlaCys: 0.912 ± 0.3
6.463AlaAsp: 6.463 ± 0.627
8.44AlaGlu: 8.44 ± 0.808
3.422AlaPhe: 3.422 ± 0.406
7.451AlaGly: 7.451 ± 0.94
1.445AlaHis: 1.445 ± 0.307
4.866AlaIle: 4.866 ± 0.557
6.919AlaLys: 6.919 ± 0.559
8.212AlaLeu: 8.212 ± 0.857
2.357AlaMet: 2.357 ± 0.444
3.422AlaAsn: 3.422 ± 0.402
3.954AlaPro: 3.954 ± 0.522
4.03AlaGln: 4.03 ± 0.554
4.79AlaArg: 4.79 ± 0.559
5.246AlaSer: 5.246 ± 0.733
4.486AlaThr: 4.486 ± 0.505
6.463AlaVal: 6.463 ± 0.756
1.369AlaTrp: 1.369 ± 0.364
2.965AlaTyr: 2.965 ± 0.506
0.0AlaXaa: 0.0 ± 0.0
Cys
0.76CysAla: 0.76 ± 0.212
0.532CysCys: 0.532 ± 0.231
0.456CysAsp: 0.456 ± 0.166
0.608CysGlu: 0.608 ± 0.202
0.38CysPhe: 0.38 ± 0.172
1.293CysGly: 1.293 ± 0.401
0.38CysHis: 0.38 ± 0.181
0.76CysIle: 0.76 ± 0.241
0.38CysLys: 0.38 ± 0.191
0.836CysLeu: 0.836 ± 0.243
0.38CysMet: 0.38 ± 0.207
0.532CysAsn: 0.532 ± 0.217
0.684CysPro: 0.684 ± 0.243
0.532CysGln: 0.532 ± 0.229
1.445CysArg: 1.445 ± 0.316
0.912CysSer: 0.912 ± 0.273
0.608CysThr: 0.608 ± 0.185
1.141CysVal: 1.141 ± 0.3
0.076CysTrp: 0.076 ± 0.073
0.152CysTyr: 0.152 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
4.714AspAla: 4.714 ± 0.5
0.912AspCys: 0.912 ± 0.358
3.574AspAsp: 3.574 ± 0.693
4.182AspGlu: 4.182 ± 0.621
2.509AspPhe: 2.509 ± 0.434
4.562AspGly: 4.562 ± 0.554
0.912AspHis: 0.912 ± 0.285
3.726AspIle: 3.726 ± 0.497
2.889AspLys: 2.889 ± 0.522
6.159AspLeu: 6.159 ± 0.783
1.597AspMet: 1.597 ± 0.342
2.433AspAsn: 2.433 ± 0.495
2.585AspPro: 2.585 ± 0.428
2.889AspGln: 2.889 ± 0.432
3.498AspArg: 3.498 ± 0.436
2.433AspSer: 2.433 ± 0.383
1.977AspThr: 1.977 ± 0.356
4.638AspVal: 4.638 ± 0.656
0.684AspTrp: 0.684 ± 0.258
1.901AspTyr: 1.901 ± 0.373
0.0AspXaa: 0.0 ± 0.0
Glu
7.375GluAla: 7.375 ± 0.634
0.608GluCys: 0.608 ± 0.214
2.585GluAsp: 2.585 ± 0.498
5.018GluGlu: 5.018 ± 0.87
2.813GluPhe: 2.813 ± 0.411
4.258GluGly: 4.258 ± 0.504
1.597GluHis: 1.597 ± 0.333
3.498GluIle: 3.498 ± 0.564
2.965GluLys: 2.965 ± 0.445
7.908GluLeu: 7.908 ± 0.981
2.129GluMet: 2.129 ± 0.366
1.597GluAsn: 1.597 ± 0.303
2.433GluPro: 2.433 ± 0.417
5.246GluGln: 5.246 ± 0.668
4.03GluArg: 4.03 ± 0.611
4.106GluSer: 4.106 ± 0.538
4.182GluThr: 4.182 ± 0.545
4.866GluVal: 4.866 ± 0.62
1.293GluTrp: 1.293 ± 0.368
2.205GluTyr: 2.205 ± 0.374
0.0GluXaa: 0.0 ± 0.0
Phe
3.117PheAla: 3.117 ± 0.548
0.684PheCys: 0.684 ± 0.284
3.193PheAsp: 3.193 ± 0.52
2.205PheGlu: 2.205 ± 0.389
1.064PhePhe: 1.064 ± 0.262
2.965PheGly: 2.965 ± 0.561
0.608PheHis: 0.608 ± 0.267
1.749PheIle: 1.749 ± 0.344
2.281PheLys: 2.281 ± 0.355
2.129PheLeu: 2.129 ± 0.404
0.988PheMet: 0.988 ± 0.239
1.521PheAsn: 1.521 ± 0.377
0.988PhePro: 0.988 ± 0.277
0.684PheGln: 0.684 ± 0.202
1.749PheArg: 1.749 ± 0.393
2.509PheSer: 2.509 ± 0.395
1.673PheThr: 1.673 ± 0.348
2.433PheVal: 2.433 ± 0.496
0.532PheTrp: 0.532 ± 0.166
1.064PheTyr: 1.064 ± 0.285
0.0PheXaa: 0.0 ± 0.0
Gly
6.539GlyAla: 6.539 ± 0.814
0.912GlyCys: 0.912 ± 0.3
4.258GlyAsp: 4.258 ± 0.542
4.714GlyGlu: 4.714 ± 0.578
2.889GlyPhe: 2.889 ± 0.477
5.55GlyGly: 5.55 ± 0.611
1.825GlyHis: 1.825 ± 0.413
3.574GlyIle: 3.574 ± 0.481
4.79GlyLys: 4.79 ± 0.564
7.299GlyLeu: 7.299 ± 0.664
2.357GlyMet: 2.357 ± 0.499
2.053GlyAsn: 2.053 ± 0.501
1.825GlyPro: 1.825 ± 0.37
2.813GlyGln: 2.813 ± 0.446
3.422GlyArg: 3.422 ± 0.504
3.574GlySer: 3.574 ± 0.457
4.486GlyThr: 4.486 ± 0.673
5.17GlyVal: 5.17 ± 0.712
1.141GlyTrp: 1.141 ± 0.247
2.661GlyTyr: 2.661 ± 0.44
0.0GlyXaa: 0.0 ± 0.0
His
1.673HisAla: 1.673 ± 0.385
0.38HisCys: 0.38 ± 0.221
0.912HisAsp: 0.912 ± 0.248
1.901HisGlu: 1.901 ± 0.321
0.76HisPhe: 0.76 ± 0.221
1.369HisGly: 1.369 ± 0.288
0.684HisHis: 0.684 ± 0.232
1.521HisIle: 1.521 ± 0.335
1.141HisLys: 1.141 ± 0.242
2.129HisLeu: 2.129 ± 0.408
0.304HisMet: 0.304 ± 0.14
1.217HisAsn: 1.217 ± 0.36
0.76HisPro: 0.76 ± 0.205
0.912HisGln: 0.912 ± 0.247
1.521HisArg: 1.521 ± 0.328
0.608HisSer: 0.608 ± 0.205
0.988HisThr: 0.988 ± 0.31
1.597HisVal: 1.597 ± 0.348
0.38HisTrp: 0.38 ± 0.215
0.836HisTyr: 0.836 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
4.79IleAla: 4.79 ± 0.575
0.76IleCys: 0.76 ± 0.204
3.117IleAsp: 3.117 ± 0.42
3.574IleGlu: 3.574 ± 0.518
0.912IlePhe: 0.912 ± 0.279
4.106IleGly: 4.106 ± 0.361
1.064IleHis: 1.064 ± 0.233
2.281IleIle: 2.281 ± 0.336
4.03IleLys: 4.03 ± 0.584
3.498IleLeu: 3.498 ± 0.519
0.836IleMet: 0.836 ± 0.236
1.901IleAsn: 1.901 ± 0.395
1.749IlePro: 1.749 ± 0.31
1.977IleGln: 1.977 ± 0.426
2.357IleArg: 2.357 ± 0.368
3.193IleSer: 3.193 ± 0.504
3.878IleThr: 3.878 ± 0.45
3.041IleVal: 3.041 ± 0.434
0.532IleTrp: 0.532 ± 0.232
1.064IleTyr: 1.064 ± 0.261
0.0IleXaa: 0.0 ± 0.0
Lys
6.615LysAla: 6.615 ± 0.757
0.532LysCys: 0.532 ± 0.213
3.802LysAsp: 3.802 ± 0.503
3.726LysGlu: 3.726 ± 0.586
1.445LysPhe: 1.445 ± 0.331
4.41LysGly: 4.41 ± 0.485
1.673LysHis: 1.673 ± 0.412
1.749LysIle: 1.749 ± 0.328
3.041LysLys: 3.041 ± 0.54
3.574LysLeu: 3.574 ± 0.499
1.749LysMet: 1.749 ± 0.372
2.585LysAsn: 2.585 ± 0.437
2.281LysPro: 2.281 ± 0.415
2.129LysGln: 2.129 ± 0.441
3.498LysArg: 3.498 ± 0.474
3.422LysSer: 3.422 ± 0.579
4.03LysThr: 4.03 ± 0.599
4.714LysVal: 4.714 ± 0.788
0.836LysTrp: 0.836 ± 0.251
1.673LysTyr: 1.673 ± 0.431
0.0LysXaa: 0.0 ± 0.0
Leu
9.276LeuAla: 9.276 ± 0.852
0.836LeuCys: 0.836 ± 0.285
5.474LeuAsp: 5.474 ± 0.518
5.855LeuGlu: 5.855 ± 0.746
3.422LeuPhe: 3.422 ± 0.453
6.615LeuGly: 6.615 ± 0.668
1.749LeuHis: 1.749 ± 0.368
3.65LeuIle: 3.65 ± 0.501
5.094LeuLys: 5.094 ± 0.689
7.299LeuLeu: 7.299 ± 0.661
1.521LeuMet: 1.521 ± 0.33
5.322LeuAsn: 5.322 ± 0.592
4.562LeuPro: 4.562 ± 0.72
4.79LeuGln: 4.79 ± 0.742
6.539LeuArg: 6.539 ± 0.69
6.007LeuSer: 6.007 ± 0.743
4.866LeuThr: 4.866 ± 0.489
4.562LeuVal: 4.562 ± 0.544
1.217LeuTrp: 1.217 ± 0.287
1.977LeuTyr: 1.977 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.813MetAla: 2.813 ± 0.601
0.76MetCys: 0.76 ± 0.202
1.445MetAsp: 1.445 ± 0.315
1.901MetGlu: 1.901 ± 0.386
0.532MetPhe: 0.532 ± 0.184
1.521MetGly: 1.521 ± 0.328
0.456MetHis: 0.456 ± 0.199
0.76MetIle: 0.76 ± 0.263
1.749MetLys: 1.749 ± 0.454
2.585MetLeu: 2.585 ± 0.473
0.608MetMet: 0.608 ± 0.276
1.369MetAsn: 1.369 ± 0.262
1.064MetPro: 1.064 ± 0.227
0.836MetGln: 0.836 ± 0.273
1.293MetArg: 1.293 ± 0.267
1.673MetSer: 1.673 ± 0.368
1.673MetThr: 1.673 ± 0.303
1.369MetVal: 1.369 ± 0.332
0.456MetTrp: 0.456 ± 0.183
0.38MetTyr: 0.38 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
3.498AsnAla: 3.498 ± 0.515
0.228AsnCys: 0.228 ± 0.116
1.901AsnAsp: 1.901 ± 0.54
2.357AsnGlu: 2.357 ± 0.373
0.456AsnPhe: 0.456 ± 0.168
3.878AsnGly: 3.878 ± 0.575
1.521AsnHis: 1.521 ± 0.303
1.901AsnIle: 1.901 ± 0.435
1.445AsnLys: 1.445 ± 0.319
3.65AsnLeu: 3.65 ± 0.535
1.141AsnMet: 1.141 ± 0.315
1.597AsnAsn: 1.597 ± 0.341
2.281AsnPro: 2.281 ± 0.436
2.129AsnGln: 2.129 ± 0.412
2.357AsnArg: 2.357 ± 0.411
2.205AsnSer: 2.205 ± 0.427
2.129AsnThr: 2.129 ± 0.395
1.977AsnVal: 1.977 ± 0.38
0.912AsnTrp: 0.912 ± 0.302
1.369AsnTyr: 1.369 ± 0.296
0.0AsnXaa: 0.0 ± 0.0
Pro
3.65ProAla: 3.65 ± 0.565
0.912ProCys: 0.912 ± 0.333
2.813ProAsp: 2.813 ± 0.546
3.65ProGlu: 3.65 ± 0.7
0.988ProPhe: 0.988 ± 0.293
2.433ProGly: 2.433 ± 0.445
0.532ProHis: 0.532 ± 0.185
2.053ProIle: 2.053 ± 0.414
1.749ProLys: 1.749 ± 0.315
3.954ProLeu: 3.954 ± 0.53
0.684ProMet: 0.684 ± 0.209
1.369ProAsn: 1.369 ± 0.266
1.064ProPro: 1.064 ± 0.31
1.977ProGln: 1.977 ± 0.741
2.053ProArg: 2.053 ± 0.551
2.129ProSer: 2.129 ± 0.371
2.357ProThr: 2.357 ± 0.413
3.117ProVal: 3.117 ± 0.555
0.76ProTrp: 0.76 ± 0.295
0.836ProTyr: 0.836 ± 0.25
0.0ProXaa: 0.0 ± 0.0
Gln
5.703GlnAla: 5.703 ± 1.063
0.532GlnCys: 0.532 ± 0.257
1.749GlnAsp: 1.749 ± 0.337
4.562GlnGlu: 4.562 ± 0.726
1.673GlnPhe: 1.673 ± 0.372
2.661GlnGly: 2.661 ± 0.419
0.988GlnHis: 0.988 ± 0.301
1.977GlnIle: 1.977 ± 0.334
2.281GlnLys: 2.281 ± 0.407
5.094GlnLeu: 5.094 ± 0.659
1.141GlnMet: 1.141 ± 0.31
1.597GlnAsn: 1.597 ± 0.394
2.205GlnPro: 2.205 ± 0.462
2.281GlnGln: 2.281 ± 0.81
2.205GlnArg: 2.205 ± 0.372
2.509GlnSer: 2.509 ± 0.282
2.509GlnThr: 2.509 ± 0.425
4.106GlnVal: 4.106 ± 0.533
0.684GlnTrp: 0.684 ± 0.247
1.445GlnTyr: 1.445 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
5.018ArgAla: 5.018 ± 0.473
0.532ArgCys: 0.532 ± 0.208
1.901ArgAsp: 1.901 ± 0.397
3.954ArgGlu: 3.954 ± 0.505
2.281ArgPhe: 2.281 ± 0.403
3.041ArgGly: 3.041 ± 0.417
1.293ArgHis: 1.293 ± 0.413
2.965ArgIle: 2.965 ± 0.525
3.878ArgLys: 3.878 ± 0.518
5.931ArgLeu: 5.931 ± 0.596
1.597ArgMet: 1.597 ± 0.404
1.901ArgAsn: 1.901 ± 0.386
2.053ArgPro: 2.053 ± 0.459
3.802ArgGln: 3.802 ± 0.658
4.03ArgArg: 4.03 ± 0.515
2.813ArgSer: 2.813 ± 0.481
2.585ArgThr: 2.585 ± 0.361
4.714ArgVal: 4.714 ± 0.454
1.369ArgTrp: 1.369 ± 0.442
2.205ArgTyr: 2.205 ± 0.419
0.0ArgXaa: 0.0 ± 0.0
Ser
5.931SerAla: 5.931 ± 0.768
0.684SerCys: 0.684 ± 0.218
3.345SerAsp: 3.345 ± 0.489
2.737SerGlu: 2.737 ± 0.419
2.281SerPhe: 2.281 ± 0.325
5.094SerGly: 5.094 ± 0.636
1.597SerHis: 1.597 ± 0.314
2.281SerIle: 2.281 ± 0.441
3.422SerLys: 3.422 ± 0.415
4.942SerLeu: 4.942 ± 0.507
2.205SerMet: 2.205 ± 0.399
2.357SerAsn: 2.357 ± 0.548
2.129SerPro: 2.129 ± 0.403
2.813SerGln: 2.813 ± 0.445
2.889SerArg: 2.889 ± 0.389
3.041SerSer: 3.041 ± 0.516
2.433SerThr: 2.433 ± 0.381
3.726SerVal: 3.726 ± 0.593
0.684SerTrp: 0.684 ± 0.205
1.673SerTyr: 1.673 ± 0.388
0.0SerXaa: 0.0 ± 0.0
Thr
5.474ThrAla: 5.474 ± 0.517
0.684ThrCys: 0.684 ± 0.234
3.422ThrAsp: 3.422 ± 0.471
3.954ThrGlu: 3.954 ± 0.544
1.597ThrPhe: 1.597 ± 0.292
4.182ThrGly: 4.182 ± 0.586
0.912ThrHis: 0.912 ± 0.24
2.813ThrIle: 2.813 ± 0.478
3.345ThrLys: 3.345 ± 0.463
4.942ThrLeu: 4.942 ± 0.502
0.836ThrMet: 0.836 ± 0.211
1.445ThrAsn: 1.445 ± 0.327
2.205ThrPro: 2.205 ± 0.458
2.737ThrGln: 2.737 ± 0.409
2.813ThrArg: 2.813 ± 0.366
3.574ThrSer: 3.574 ± 0.534
2.965ThrThr: 2.965 ± 0.51
3.802ThrVal: 3.802 ± 0.555
0.912ThrTrp: 0.912 ± 0.207
1.597ThrTyr: 1.597 ± 0.307
0.0ThrXaa: 0.0 ± 0.0
Val
6.615ValAla: 6.615 ± 1.05
0.988ValCys: 0.988 ± 0.274
4.562ValAsp: 4.562 ± 0.622
4.03ValGlu: 4.03 ± 0.525
2.889ValPhe: 2.889 ± 0.377
3.802ValGly: 3.802 ± 0.462
1.445ValHis: 1.445 ± 0.347
4.79ValIle: 4.79 ± 0.648
3.802ValLys: 3.802 ± 0.565
6.007ValLeu: 6.007 ± 0.761
1.825ValMet: 1.825 ± 0.358
3.117ValAsn: 3.117 ± 0.529
2.737ValPro: 2.737 ± 0.436
2.889ValGln: 2.889 ± 0.431
3.954ValArg: 3.954 ± 0.636
3.422ValSer: 3.422 ± 0.584
4.638ValThr: 4.638 ± 0.622
5.17ValVal: 5.17 ± 0.718
1.445ValTrp: 1.445 ± 0.291
1.901ValTyr: 1.901 ± 0.405
0.0ValXaa: 0.0 ± 0.0
Trp
1.217TrpAla: 1.217 ± 0.257
0.152TrpCys: 0.152 ± 0.115
1.369TrpAsp: 1.369 ± 0.323
1.217TrpGlu: 1.217 ± 0.316
0.988TrpPhe: 0.988 ± 0.27
0.988TrpGly: 0.988 ± 0.212
0.38TrpHis: 0.38 ± 0.201
0.76TrpIle: 0.76 ± 0.244
0.988TrpLys: 0.988 ± 0.282
1.293TrpLeu: 1.293 ± 0.232
0.152TrpMet: 0.152 ± 0.097
0.684TrpAsn: 0.684 ± 0.208
0.532TrpPro: 0.532 ± 0.204
0.304TrpGln: 0.304 ± 0.148
1.064TrpArg: 1.064 ± 0.315
1.293TrpSer: 1.293 ± 0.279
0.532TrpThr: 0.532 ± 0.187
1.597TrpVal: 1.597 ± 0.384
0.38TrpTrp: 0.38 ± 0.168
0.076TrpTyr: 0.076 ± 0.073
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.433TyrAla: 2.433 ± 0.474
0.304TyrCys: 0.304 ± 0.141
2.433TyrAsp: 2.433 ± 0.368
2.129TyrGlu: 2.129 ± 0.508
0.836TyrPhe: 0.836 ± 0.245
1.749TyrGly: 1.749 ± 0.401
0.532TyrHis: 0.532 ± 0.231
0.988TyrIle: 0.988 ± 0.344
1.293TyrLys: 1.293 ± 0.282
3.193TyrLeu: 3.193 ± 0.48
0.76TyrMet: 0.76 ± 0.205
0.912TyrAsn: 0.912 ± 0.251
0.988TyrPro: 0.988 ± 0.339
2.129TyrGln: 2.129 ± 0.47
2.357TyrArg: 2.357 ± 0.377
1.597TyrSer: 1.597 ± 0.385
1.217TyrThr: 1.217 ± 0.286
1.825TyrVal: 1.825 ± 0.299
0.304TyrTrp: 0.304 ± 0.149
0.988TyrTyr: 0.988 ± 0.255
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (13153 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski