Psittacid herpesvirus 1 (isolate Amazon parrot/-/97-0001/1997) (PsHV-1) (Pacheco s disease virus)
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q6UDF8|GG_PSHV1 Glycoprotein G OS=Psittacid herpesvirus 1 (isolate Amazon parrot/-/97-0001/1997) OX=670426 GN=gG PE=3 SV=1
MM1 pKa = 7.71 LWPAALVAMFALAARR16 pKa = 11.84 ARR18 pKa = 11.84 EE19 pKa = 4.04 IDD21 pKa = 3.64 NVTCSVVYY29 pKa = 9.66 GSNVARR35 pKa = 11.84 ISKK38 pKa = 9.95 DD39 pKa = 2.97 YY40 pKa = 10.5 WLAQEE45 pKa = 4.73 GSLVSFMTFEE55 pKa = 4.43 NDD57 pKa = 3.13 DD58 pKa = 3.47 PVYY61 pKa = 10.82 FFMGRR66 pKa = 11.84 ALGTGASGGKK76 pKa = 9.21 YY77 pKa = 9.89 LYY79 pKa = 10.49 RR80 pKa = 11.84 VTRR83 pKa = 11.84 DD84 pKa = 2.89 NNTNVSHH91 pKa = 5.86 VQTSLFIPEE100 pKa = 4.62 HH101 pKa = 5.8 FNGGILLDD109 pKa = 3.48 MGSNYY114 pKa = 8.76 THH116 pKa = 7.6 DD117 pKa = 4.73 AEE119 pKa = 4.61 TDD121 pKa = 3.68 PEE123 pKa = 4.27 FLNARR128 pKa = 11.84 YY129 pKa = 9.16 LVVNDD134 pKa = 4.1 TEE136 pKa = 4.73 DD137 pKa = 3.35 SVRR140 pKa = 11.84 EE141 pKa = 4.05 EE142 pKa = 4.17 TLGPCPEE149 pKa = 4.19 MLRR152 pKa = 11.84 TLDD155 pKa = 3.31 VSGRR159 pKa = 11.84 TTLLYY164 pKa = 9.85 PSRR167 pKa = 11.84 YY168 pKa = 8.46 LHH170 pKa = 6.78 ARR172 pKa = 11.84 DD173 pKa = 3.26 RR174 pKa = 11.84 MQVEE178 pKa = 4.38 KK179 pKa = 10.51 TMDD182 pKa = 3.25 TAKK185 pKa = 10.6 CVSTSVNLDD194 pKa = 3.17 ATVVFSVDD202 pKa = 3.76 FPFSEE207 pKa = 4.29 RR208 pKa = 11.84 AILRR212 pKa = 11.84 VTQTFYY218 pKa = 10.99 PDD220 pKa = 4.09 DD221 pKa = 4.31 RR222 pKa = 11.84 PQKK225 pKa = 10.03 HH226 pKa = 6.33 LLYY229 pKa = 10.46 LASQGGKK236 pKa = 6.3 TVHH239 pKa = 5.93 YY240 pKa = 9.7 SEE242 pKa = 5.31 VSATVTPLPGGNASFNVTIEE262 pKa = 4.26 FTDD265 pKa = 3.63 QPDD268 pKa = 3.48 DD269 pKa = 3.76 TTIHH273 pKa = 6.25 LWVSPKK279 pKa = 10.6 GINDD283 pKa = 3.81 DD284 pKa = 3.77 PDD286 pKa = 3.73 AVSAYY291 pKa = 10.5 YY292 pKa = 9.98 ILNSLSSYY300 pKa = 10.27 DD301 pKa = 3.7 DD302 pKa = 4.34 PYY304 pKa = 11.04 AHH306 pKa = 7.22 EE307 pKa = 5.43 EE308 pKa = 3.97 ISCDD312 pKa = 3.18 SVYY315 pKa = 11.19 QNIVMVLGSVVNITSPDD332 pKa = 3.88 PLPRR336 pKa = 11.84 APDD339 pKa = 3.38 YY340 pKa = 11.14 SYY342 pKa = 8.83 PTDD345 pKa = 4.01 DD346 pKa = 4.17 PAGEE350 pKa = 4.33 TTEE353 pKa = 5.03 SAVTWLEE360 pKa = 3.83 ATAQTADD367 pKa = 3.53 EE368 pKa = 4.52 ATTPLAAHH376 pKa = 6.28 ATDD379 pKa = 4.67 GYY381 pKa = 11.16 EE382 pKa = 4.05 PTTAAATLSTTEE394 pKa = 5.34 DD395 pKa = 3.35 LTQEE399 pKa = 4.1 TSTPTVTTVIDD410 pKa = 3.85 PTSGAVTTEE419 pKa = 3.52 SRR421 pKa = 11.84 TTEE424 pKa = 3.65 GTAANVATTEE434 pKa = 3.94 AAGTEE439 pKa = 4.34 GQNQEE444 pKa = 3.77 ATTAGGPTNAATTLGHH460 pKa = 5.1 QTIEE464 pKa = 3.96 AATVEE469 pKa = 4.52 DD470 pKa = 3.99 STTTARR476 pKa = 11.84 AAEE479 pKa = 4.57 YY480 pKa = 7.18 PTPTTTTVEE489 pKa = 3.96 PRR491 pKa = 11.84 PAGGTTIEE499 pKa = 5.25 DD500 pKa = 4.01 PTDD503 pKa = 3.42 SPAIEE508 pKa = 4.73 DD509 pKa = 3.88 TTTPTTTAAKK519 pKa = 8.82 VTALKK524 pKa = 9.03 TAAAEE529 pKa = 4.57 GPTPCVTTYY538 pKa = 10.04 TGSDD542 pKa = 3.19 TEE544 pKa = 4.4 AAQSATSISDD554 pKa = 3.11 AVTPEE559 pKa = 4.15 DD560 pKa = 4.11 LTPEE564 pKa = 4.05 TTTPVDD570 pKa = 3.12 WSVTSTYY577 pKa = 10.7 ISVADD582 pKa = 4.24 GTSMPAPTPSAVAHH596 pKa = 6.01 EE597 pKa = 4.99 ASTTPAPTATAVPTSHH613 pKa = 6.41 TPKK616 pKa = 9.38 PQEE619 pKa = 4.15 STSTPSRR626 pKa = 11.84 APTTGVAPVTANSLSPAATSSEE648 pKa = 4.09 RR649 pKa = 11.84 IVILNTATASSGPGASTGATTAPISPPWSASPAGDD684 pKa = 3.34 GVTTSAARR692 pKa = 11.84 TLEE695 pKa = 4.05 PSSTRR700 pKa = 11.84 KK701 pKa = 9.66 AVAAEE706 pKa = 4.13 STTAADD712 pKa = 4.41 DD713 pKa = 3.8 VATSEE718 pKa = 4.92 LGSGDD723 pKa = 4.02 YY724 pKa = 10.71 GDD726 pKa = 5.1 HH727 pKa = 5.77 ATEE730 pKa = 4.32 PPRR733 pKa = 11.84 IVITNPPGITTLHH746 pKa = 6.78 DD747 pKa = 3.78 ADD749 pKa = 4.81 AAEE752 pKa = 4.43 EE753 pKa = 4.63 DD754 pKa = 3.81 PWPTRR759 pKa = 11.84 PLYY762 pKa = 10.67 SVNIVNATLTANGMLTATCMAAAKK786 pKa = 9.38 AKK788 pKa = 10.28 HH789 pKa = 6.34 AITFTWHH796 pKa = 5.63 VGSNMVPAITGPPEE810 pKa = 4.42 PGLMFNGNRR819 pKa = 11.84 AWSSRR824 pKa = 11.84 LQTVEE829 pKa = 3.96 YY830 pKa = 9.92 GISPSARR837 pKa = 11.84 LACMACTVPPAQRR850 pKa = 11.84 YY851 pKa = 6.88 CAHH854 pKa = 7.07 DD855 pKa = 3.55 VAVVARR861 pKa = 11.84 HH862 pKa = 6.44 DD863 pKa = 3.85 RR864 pKa = 11.84 LEE866 pKa = 5.66 LDD868 pKa = 3.51 MQVDD872 pKa = 4.12 VATVSVVCSGLDD884 pKa = 3.53 GVEE887 pKa = 4.05 SEE889 pKa = 5.42 PYY891 pKa = 10.11 FVWTANGRR899 pKa = 11.84 PVPLGSVRR907 pKa = 11.84 TKK909 pKa = 10.65 RR910 pKa = 11.84 IPNDD914 pKa = 3.17 YY915 pKa = 7.85 GTPARR920 pKa = 11.84 WQSAIHH926 pKa = 6.45 ISRR929 pKa = 11.84 FFVPAGHH936 pKa = 6.76 RR937 pKa = 11.84 DD938 pKa = 3.6 VYY940 pKa = 11.22 EE941 pKa = 4.26 CTATLASGEE950 pKa = 4.38 TIKK953 pKa = 10.26 ATKK956 pKa = 9.46 NWSNTDD962 pKa = 3.37 YY963 pKa = 11.46 LALQKK968 pKa = 10.62 NAAARR973 pKa = 11.84 STFVVAGGITAFAVAEE989 pKa = 4.12 LLL991 pKa = 4.14
Molecular weight: 104.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.397
IPC2_protein 4.457
IPC_protein 4.444
Toseland 4.279
ProMoST 4.558
Dawson 4.406
Bjellqvist 4.546
Wikipedia 4.279
Rodwell 4.279
Grimsley 4.19
Solomon 4.406
Lehninger 4.355
Nozaki 4.507
DTASelect 4.698
Thurlkill 4.291
EMBOSS 4.304
Sillero 4.558
Patrickios 3.376
IPC_peptide 4.406
IPC2_peptide 4.546
IPC2.peptide.svr19 4.491
Protein with the highest isoelectric point:
>sp|Q6UDK9|KITH_PSHV1 Thymidine kinase OS=Psittacid herpesvirus 1 (isolate Amazon parrot/-/97-0001/1997) OX=670426 GN=TK PE=3 SV=1
MM1 pKa = 7.39 PRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 GPRR9 pKa = 11.84 CDD11 pKa = 3.54 PSSALAVDD19 pKa = 3.95 VAAAVAAATSRR30 pKa = 11.84 TRR32 pKa = 11.84 AQKK35 pKa = 9.49 VRR37 pKa = 11.84 KK38 pKa = 8.76 AKK40 pKa = 10.25 KK41 pKa = 8.15 VLAANGHH48 pKa = 5.21 KK49 pKa = 8.31 WHH51 pKa = 6.99 RR52 pKa = 11.84 RR53 pKa = 11.84 AKK55 pKa = 8.93 TPVNKK60 pKa = 8.94 RR61 pKa = 11.84 TLVAARR67 pKa = 11.84 LLAGVRR73 pKa = 11.84 CHH75 pKa = 5.73 NRR77 pKa = 11.84 FYY79 pKa = 11.26 GALVSSFEE87 pKa = 3.96 TAYY90 pKa = 11.04 CMNHH94 pKa = 4.89 RR95 pKa = 11.84 HH96 pKa = 5.85 GSDD99 pKa = 2.98 GLAATIIAEE108 pKa = 4.19 ACGAVPVCSLNTTIMFEE125 pKa = 4.22 VNLGGRR131 pKa = 11.84 RR132 pKa = 11.84 PDD134 pKa = 4.31 CICVLDD140 pKa = 3.85 RR141 pKa = 11.84 AGVAGAPRR149 pKa = 11.84 EE150 pKa = 4.36 SVCLIVEE157 pKa = 4.72 LKK159 pKa = 8.72 TCRR162 pKa = 11.84 FSRR165 pKa = 11.84 SALTEE170 pKa = 4.14 SKK172 pKa = 10.4 KK173 pKa = 10.38 NQYY176 pKa = 8.5 ATGLRR181 pKa = 11.84 QLRR184 pKa = 11.84 DD185 pKa = 3.36 SAKK188 pKa = 9.91 IICDD192 pKa = 3.45 VAVPGADD199 pKa = 3.05 FVHH202 pKa = 6.57 IVPVLVFVAQRR213 pKa = 11.84 SMRR216 pKa = 11.84 IMDD219 pKa = 3.03 VRR221 pKa = 11.84 KK222 pKa = 8.99 FNEE225 pKa = 3.8 RR226 pKa = 11.84 VVRR229 pKa = 11.84 ANAEE233 pKa = 4.18 LLRR236 pKa = 11.84 LRR238 pKa = 11.84 LAALGVYY245 pKa = 9.63 SRR247 pKa = 11.84 SFAAAPQRR255 pKa = 11.84 LQSAQDD261 pKa = 3.2 RR262 pKa = 11.84 CHH264 pKa = 6.78 DD265 pKa = 3.68 HH266 pKa = 7.13 AATPKK271 pKa = 8.0 PTPAVVPSPPAPPNPAPPQSRR292 pKa = 11.84 VGFGSALAMATAVMLGEE309 pKa = 4.21 YY310 pKa = 10.27 ARR312 pKa = 11.84 EE313 pKa = 4.06 NKK315 pKa = 9.73 PPRR318 pKa = 4.13
Molecular weight: 34.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.619
IPC_protein 10.555
Toseland 10.847
ProMoST 10.789
Dawson 10.906
Bjellqvist 10.672
Wikipedia 11.155
Rodwell 11.023
Grimsley 10.935
Solomon 11.096
Lehninger 11.052
Nozaki 10.847
DTASelect 10.657
Thurlkill 10.833
EMBOSS 11.257
Sillero 10.847
Patrickios 10.73
IPC_peptide 11.096
IPC2_peptide 10.014
IPC2.peptide.svr19 8.773
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
1
72
40367
47
3195
560.7
61.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.906 ± 0.355
2.049 ± 0.126
5.757 ± 0.23
5.854 ± 0.238
3.651 ± 0.169
6.934 ± 0.178
2.059 ± 0.074
3.647 ± 0.137
3.518 ± 0.209
9.084 ± 0.221
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.356 ± 0.121
2.918 ± 0.129
5.965 ± 0.297
2.75 ± 0.13
7.62 ± 0.252
7.578 ± 0.235
5.638 ± 0.291
6.76 ± 0.208
1.147 ± 0.051
2.809 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here