Planktothrix phage PaV-LD
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 142 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9BWC2|G9BWC2_9CAUD Uncharacterized protein OS=Planktothrix phage PaV-LD OX=994601 GN=PaVLD_ORF019R PE=4 SV=1
MM1 pKa = 8.17 LEE3 pKa = 3.85 IYY5 pKa = 10.19 EE6 pKa = 4.17 YY7 pKa = 10.6 DD8 pKa = 3.2 QSNIDD13 pKa = 3.82 EE14 pKa = 4.66 SWFDD18 pKa = 3.31 WSGGLWWPDD27 pKa = 3.19 FEE29 pKa = 4.35 EE30 pKa = 5.92 AGIEE34 pKa = 4.08 KK35 pKa = 10.61 CFVAWKK41 pKa = 9.82 GEE43 pKa = 4.13 EE44 pKa = 4.0 VVGFQTVNGSYY55 pKa = 10.93 DD56 pKa = 3.48 CVAIEE61 pKa = 5.37 AKK63 pKa = 10.63 YY64 pKa = 9.75 QGQGIGRR71 pKa = 11.84 ALVEE75 pKa = 3.59 QSGCYY80 pKa = 9.74 RR81 pKa = 11.84 PDD83 pKa = 3.28 RR84 pKa = 11.84 NEE86 pKa = 4.4 CPEE89 pKa = 4.22 FWAKK93 pKa = 8.99 MAEE96 pKa = 4.22 EE97 pKa = 4.46 FGNN100 pKa = 3.64
Molecular weight: 11.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.841
IPC2_protein 4.088
IPC_protein 3.973
Toseland 3.808
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.783
Rodwell 3.808
Grimsley 3.719
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.139
Thurlkill 3.834
EMBOSS 3.808
Sillero 4.075
Patrickios 0.896
IPC_peptide 3.91
IPC2_peptide 4.062
IPC2.peptide.svr19 3.974
Protein with the highest isoelectric point:
>tr|G9BWG5|G9BWG5_9CAUD Uncharacterized protein OS=Planktothrix phage PaV-LD OX=994601 GN=PaVLD_ORF062R PE=4 SV=1
MM1 pKa = 7.34 TFILYY6 pKa = 9.28 RR7 pKa = 11.84 QKK9 pKa = 11.21 SRR11 pKa = 11.84 FNAPPTEE18 pKa = 4.09 IQAKK22 pKa = 9.55 LAIAQAHH29 pKa = 5.75 LNIGRR34 pKa = 11.84 SKK36 pKa = 11.3 LNN38 pKa = 3.3
Molecular weight: 4.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 10.116
IPC_protein 11.052
Toseland 11.242
ProMoST 11.228
Dawson 11.286
Bjellqvist 11.067
Wikipedia 11.564
Rodwell 11.506
Grimsley 11.316
Solomon 11.535
Lehninger 11.491
Nozaki 11.213
DTASelect 11.067
Thurlkill 11.228
EMBOSS 11.667
Sillero 11.228
Patrickios 11.33
IPC_peptide 11.55
IPC2_peptide 10.145
IPC2.peptide.svr19 8.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
142
0
142
28419
35
1584
200.1
22.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.83 ± 0.232
0.968 ± 0.077
5.722 ± 0.193
7.252 ± 0.221
3.709 ± 0.153
6.643 ± 0.187
1.358 ± 0.11
6.7 ± 0.192
7.625 ± 0.407
8.459 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.041 ± 0.103
4.954 ± 0.125
4.465 ± 0.187
4.441 ± 0.219
4.757 ± 0.153
7.203 ± 0.168
5.94 ± 0.222
6.133 ± 0.145
1.552 ± 0.117
3.248 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here