Lentzea albidocapillata
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8155 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W2F6X4|A0A1W2F6X4_9PSEU Polar amino acid transport system substrate-binding protein OS=Lentzea albidocapillata OX=40571 GN=SAMN05660733_05210 PE=4 SV=1
MM1 pKa = 7.77 RR2 pKa = 11.84 KK3 pKa = 9.01 NVKK6 pKa = 9.24 TGLLAAAVATGLVLAGPTASANTSAFHH33 pKa = 6.32 PFGMGGTEE41 pKa = 4.42 HH42 pKa = 6.01 QMAWEE47 pKa = 4.21 WFSHH51 pKa = 5.04 QAAAYY56 pKa = 8.61 AAAGHH61 pKa = 6.6 GGHH64 pKa = 6.42 SGDD67 pKa = 4.62 GGDD70 pKa = 4.13 AGSANTATTGDD81 pKa = 4.04 SGDD84 pKa = 4.27 SGDD87 pKa = 5.46 SGDD90 pKa = 4.18 TGHH93 pKa = 6.59 NSAWVMCVHH102 pKa = 6.04 SHH104 pKa = 6.89 CEE106 pKa = 3.65 ADD108 pKa = 3.84 ADD110 pKa = 4.48 SGDD113 pKa = 4.1 SGDD116 pKa = 3.99 TSTGDD121 pKa = 3.09 TGNAANNSSTNGGDD135 pKa = 3.23 TGHH138 pKa = 7.01 GGNGGDD144 pKa = 4.31 ADD146 pKa = 4.26 ADD148 pKa = 3.85 AEE150 pKa = 4.34 NAVATIWDD158 pKa = 3.74 EE159 pKa = 4.46 DD160 pKa = 4.29 GEE162 pKa = 5.22 HH163 pKa = 6.83 GDD165 pKa = 4.01 EE166 pKa = 5.06 DD167 pKa = 3.74 NSSAHH172 pKa = 6.5 SEE174 pKa = 4.15 DD175 pKa = 3.79 STHH178 pKa = 7.07 GDD180 pKa = 3.55 DD181 pKa = 4.18 STHH184 pKa = 6.65 HH185 pKa = 6.95 GDD187 pKa = 3.69 STHH190 pKa = 6.81 HH191 pKa = 6.9 GDD193 pKa = 3.89 STHH196 pKa = 6.9 HH197 pKa = 6.95 GDD199 pKa = 4.36 NEE201 pKa = 4.17 ATASATAGHH210 pKa = 6.7 GGDD213 pKa = 4.21 SGDD216 pKa = 3.9 GGDD219 pKa = 3.78 AVSSNTATSGDD230 pKa = 3.89 SGDD233 pKa = 4.47 SGDD236 pKa = 5.46 SGDD239 pKa = 4.18 TGHH242 pKa = 6.59 NSAWVMCVHH251 pKa = 6.84 SDD253 pKa = 4.14 CEE255 pKa = 4.26 VGTDD259 pKa = 3.97 SGDD262 pKa = 3.64 SGDD265 pKa = 3.99 TSTGDD270 pKa = 3.09 TGNAANNSSTNGGDD284 pKa = 3.23 TGHH287 pKa = 7.01 GGNGGDD293 pKa = 3.9 ADD295 pKa = 4.02 AAAGTSTAVEE305 pKa = 3.88 WDD307 pKa = 3.45 DD308 pKa = 5.64 DD309 pKa = 4.09 EE310 pKa = 5.0 NAAHH314 pKa = 7.07 GDD316 pKa = 3.93 SEE318 pKa = 4.33 ATAAATAGNGGSSGAGGDD336 pKa = 3.28 AVSVNTATTGDD347 pKa = 3.94 SGDD350 pKa = 4.31 SGDD353 pKa = 5.21 SGNTGHH359 pKa = 6.66 NSAWVMCAHH368 pKa = 7.13 SICLTSTDD376 pKa = 4.32 SGDD379 pKa = 4.03 SGDD382 pKa = 3.99 TSTGDD387 pKa = 3.12 TGNATNNSSTNGGDD401 pKa = 3.23 TGHH404 pKa = 7.01 GGNGGDD410 pKa = 4.26 ADD412 pKa = 5.52 AMATDD417 pKa = 4.52 LNEE420 pKa = 4.63 GEE422 pKa = 5.09 DD423 pKa = 3.89 DD424 pKa = 3.63
Molecular weight: 40.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.719
IPC_protein 3.783
Toseland 3.541
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.605
Grimsley 3.439
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.215
Thurlkill 3.605
EMBOSS 3.77
Sillero 3.91
Patrickios 0.998
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A1W2EP90|A0A1W2EP90_9PSEU Alpha-galactosidase OS=Lentzea albidocapillata OX=40571 GN=SAMN05660733_04216 PE=3 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.53 GKK5 pKa = 8.66 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 8.7 THH19 pKa = 5.15 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AILAARR37 pKa = 11.84 RR38 pKa = 11.84 SKK40 pKa = 10.82 GRR42 pKa = 11.84 ATLSAA47 pKa = 4.08
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8155
0
8155
2634138
39
12847
323.0
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.514 ± 0.035
0.797 ± 0.009
5.942 ± 0.025
5.614 ± 0.024
3.043 ± 0.016
8.871 ± 0.027
2.245 ± 0.014
3.605 ± 0.021
2.522 ± 0.026
10.416 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.847 ± 0.012
2.229 ± 0.025
5.549 ± 0.024
3.029 ± 0.019
7.457 ± 0.032
5.518 ± 0.019
6.047 ± 0.031
9.154 ± 0.033
1.589 ± 0.013
2.011 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here