Porcine stool-associated circular virus
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A341ZMI4|A0A341ZMI4_9VIRU Putative capsid protein OS=Porcine stool-associated circular virus OX=1843773 PE=4 SV=1
MM1 pKa = 6.78 ATNYY5 pKa = 9.85 VKK7 pKa = 10.65 ARR9 pKa = 11.84 YY10 pKa = 8.8 QEE12 pKa = 4.35 IYY14 pKa = 11.04 DD15 pKa = 4.09 FGTQAGKK22 pKa = 6.91 TTILGVHH29 pKa = 6.28 SPTGNKK35 pKa = 7.74 VQEE38 pKa = 4.04 MLGGFFRR45 pKa = 11.84 QFRR48 pKa = 11.84 KK49 pKa = 10.1 YY50 pKa = 9.85 RR51 pKa = 11.84 YY52 pKa = 8.73 SGCTVTMVPAAQLPADD68 pKa = 4.06 PLQVSFEE75 pKa = 4.22 AGALTIDD82 pKa = 4.33 PRR84 pKa = 11.84 DD85 pKa = 3.65 MLNPILFHH93 pKa = 6.25 GAHH96 pKa = 6.16 GEE98 pKa = 4.24 SLNEE102 pKa = 3.91 ALNLIYY108 pKa = 9.83 NTSTAEE114 pKa = 4.15 MVDD117 pKa = 3.45 GASVKK122 pKa = 10.16 EE123 pKa = 3.93 IRR125 pKa = 11.84 DD126 pKa = 3.51 QFSISSWGSEE136 pKa = 3.56 NAYY139 pKa = 10.65 YY140 pKa = 10.51 SALCDD145 pKa = 3.51 PSFKK149 pKa = 10.82 KK150 pKa = 10.77 FGIQSGAKK158 pKa = 8.81 VNLRR162 pKa = 11.84 PMVHH166 pKa = 6.0 PVVLNHH172 pKa = 6.9 AMLPSDD178 pKa = 4.84 SGVEE182 pKa = 3.71 YY183 pKa = 11.03 DD184 pKa = 4.06 NASNHH189 pKa = 6.03 YY190 pKa = 10.91 DD191 pKa = 3.35 MASDD195 pKa = 4.12 GVGQILGDD203 pKa = 3.58 NRR205 pKa = 11.84 IGQGAIGNIAEE216 pKa = 4.23 VSGAEE221 pKa = 4.01 AVSVVPRR228 pKa = 11.84 QMFTSGLRR236 pKa = 11.84 PLGWLNTTQFAAPINVPNGSTNIDD260 pKa = 3.0 WFLSYY265 pKa = 10.97 SYY267 pKa = 10.28 IPKK270 pKa = 10.1 IMMGVFILPPSYY282 pKa = 9.11 TQEE285 pKa = 3.69 MYY287 pKa = 10.42 FRR289 pKa = 11.84 LIINHH294 pKa = 5.37 YY295 pKa = 10.77 FEE297 pKa = 5.98 FKK299 pKa = 10.75 DD300 pKa = 4.02 FSTALTYY307 pKa = 11.03 SPDD310 pKa = 3.54 GARR313 pKa = 11.84 LVYY316 pKa = 10.36 TEE318 pKa = 5.24 TIPDD322 pKa = 3.85 PSGGASLSSASLDD335 pKa = 3.67 VEE337 pKa = 4.18 NGTIAKK343 pKa = 6.66 TTDD346 pKa = 2.97 GVFF349 pKa = 3.16
Molecular weight: 38.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.923
IPC2_protein 5.054
IPC_protein 5.003
Toseland 4.978
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.143
Wikipedia 4.914
Rodwell 4.927
Grimsley 4.902
Solomon 5.016
Lehninger 4.978
Nozaki 5.156
DTASelect 5.334
Thurlkill 4.991
EMBOSS 4.978
Sillero 5.219
Patrickios 3.439
IPC_peptide 5.029
IPC2_peptide 5.219
IPC2.peptide.svr19 5.165
Protein with the highest isoelectric point:
>tr|A0A341ZMI4|A0A341ZMI4_9VIRU Putative capsid protein OS=Porcine stool-associated circular virus OX=1843773 PE=4 SV=1
MM1 pKa = 6.24 QTYY4 pKa = 9.74 MMTIPRR10 pKa = 11.84 TVSKK14 pKa = 10.35 RR15 pKa = 11.84 ALRR18 pKa = 11.84 IMIEE22 pKa = 4.22 KK23 pKa = 10.03 NDD25 pKa = 3.84 CKK27 pKa = 10.6 KK28 pKa = 8.7 WTIGKK33 pKa = 9.42 EE34 pKa = 3.81 KK35 pKa = 10.49 GKK37 pKa = 10.28 NGYY40 pKa = 7.38 EE41 pKa = 3.45 HH42 pKa = 5.57 WQIRR46 pKa = 11.84 IEE48 pKa = 4.1 TSNDD52 pKa = 3.27 DD53 pKa = 3.58 FFEE56 pKa = 3.99 WMQDD60 pKa = 3.68 HH61 pKa = 7.09 IPTAHH66 pKa = 6.76 IEE68 pKa = 4.17 KK69 pKa = 10.0 SDD71 pKa = 3.77 SGVDD75 pKa = 3.14 EE76 pKa = 4.55 CRR78 pKa = 11.84 YY79 pKa = 6.09 EE80 pKa = 4.29 TKK82 pKa = 10.18 EE83 pKa = 4.03 GQYY86 pKa = 8.7 VTYY89 pKa = 10.42 SDD91 pKa = 4.24 RR92 pKa = 11.84 VQNLMQRR99 pKa = 11.84 YY100 pKa = 8.21 GAFRR104 pKa = 11.84 PNQKK108 pKa = 9.94 RR109 pKa = 11.84 AMQALEE115 pKa = 4.08 ATNDD119 pKa = 3.53 RR120 pKa = 11.84 QVVVWYY126 pKa = 10.21 DD127 pKa = 3.2 EE128 pKa = 4.14 TGNVGKK134 pKa = 10.24 SWFTGALWEE143 pKa = 4.56 RR144 pKa = 11.84 GLAYY148 pKa = 7.93 VTPPTIDD155 pKa = 3.16 TVKK158 pKa = 11.09 GLIQWVASCYY168 pKa = 9.78 IDD170 pKa = 4.44 GGWRR174 pKa = 11.84 PYY176 pKa = 11.0 VIIDD180 pKa = 4.19 VPRR183 pKa = 11.84 SWKK186 pKa = 9.71 WSEE189 pKa = 3.8 QLYY192 pKa = 10.74 SAIEE196 pKa = 4.22 SIKK199 pKa = 11.0 DD200 pKa = 3.09 GLIYY204 pKa = 9.1 DD205 pKa = 4.01 TRR207 pKa = 11.84 YY208 pKa = 8.56 HH209 pKa = 6.86 SRR211 pKa = 11.84 MINIRR216 pKa = 11.84 GVKK219 pKa = 9.03 VLVMTNTMPKK229 pKa = 9.86 LDD231 pKa = 4.6 KK232 pKa = 10.62 LSKK235 pKa = 10.6 DD236 pKa = 3.0 RR237 pKa = 11.84 WCICTFF243 pKa = 3.64
Molecular weight: 28.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.845
IPC2_protein 8.024
IPC_protein 7.966
Toseland 7.951
ProMoST 8.419
Dawson 8.668
Bjellqvist 8.829
Wikipedia 8.682
Rodwell 8.726
Grimsley 7.834
Solomon 8.814
Lehninger 8.799
Nozaki 8.946
DTASelect 8.639
Thurlkill 8.712
EMBOSS 8.858
Sillero 8.96
Patrickios 4.342
IPC_peptide 8.814
IPC2_peptide 7.673
IPC2.peptide.svr19 7.809
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
592
243
349
296.0
33.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.588 ± 1.58
1.182 ± 0.559
5.743 ± 0.538
4.899 ± 0.551
3.885 ± 1.168
7.77 ± 1.021
1.858 ± 0.136
6.757 ± 0.679
4.899 ± 1.603
6.081 ± 0.993
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.716 ± 0.255
4.73 ± 0.656
4.73 ± 0.919
4.054 ± 0.302
4.899 ± 1.34
7.432 ± 1.331
6.757 ± 0.416
6.588 ± 0.002
2.365 ± 1.382
5.068 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here