Winogradskyella aurantia

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Winogradskyella

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A265UPP2|A0A265UPP2_9FLAO Uncharacterized protein OS=Winogradskyella aurantia OX=1915063 GN=CA834_13060 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.19NYY4 pKa = 10.15IKK6 pKa = 9.6ITIVAVLCLFIQACQEE22 pKa = 3.72EE23 pKa = 4.65DD24 pKa = 3.48QEE26 pKa = 4.53FGPVVAPTNIDD37 pKa = 3.48LQLTYY42 pKa = 10.76LDD44 pKa = 5.82DD45 pKa = 4.35IDD47 pKa = 5.97GDD49 pKa = 4.34GVLDD53 pKa = 3.93EE54 pKa = 4.73VDD56 pKa = 3.9APGLGSGFVKK66 pKa = 9.49FTATADD72 pKa = 3.48NALSFVYY79 pKa = 9.76EE80 pKa = 4.15VNNIRR85 pKa = 11.84TTAVNGKK92 pKa = 8.24ATLVFSTLGLNTYY105 pKa = 9.44NATVIAYY112 pKa = 6.97GTGGASSSQTIQLDD126 pKa = 3.64VLSTFEE132 pKa = 4.46VPAEE136 pKa = 4.65LITMLTNDD144 pKa = 3.45SQRR147 pKa = 11.84TWRR150 pKa = 11.84IKK152 pKa = 11.05AEE154 pKa = 4.24VNNHH158 pKa = 6.31FGLGPVGGGFNEE170 pKa = 4.9FFQAPAFDD178 pKa = 4.03KK179 pKa = 10.83EE180 pKa = 4.61GVGMYY185 pKa = 10.2DD186 pKa = 3.26DD187 pKa = 5.7RR188 pKa = 11.84YY189 pKa = 10.27IFNVDD194 pKa = 2.96GTFTHH199 pKa = 6.5ITDD202 pKa = 4.01NTNDD206 pKa = 3.92DD207 pKa = 3.98PDD209 pKa = 4.06VNPEE213 pKa = 3.66GTVFGRR219 pKa = 11.84EE220 pKa = 3.91VLIDD224 pKa = 3.76EE225 pKa = 4.92LNGPGSGTSNGADD238 pKa = 2.88IEE240 pKa = 4.36NYY242 pKa = 9.68PYY244 pKa = 10.96SDD246 pKa = 3.93YY247 pKa = 11.36SEE249 pKa = 4.23SWFITGPGGVEE260 pKa = 3.96TLNLTGIGFLGYY272 pKa = 8.53YY273 pKa = 9.35TGGDD277 pKa = 3.01HH278 pKa = 6.95TYY280 pKa = 9.94IIEE283 pKa = 4.11SRR285 pKa = 11.84SINEE289 pKa = 3.85MVVRR293 pKa = 11.84TTDD296 pKa = 3.2GNGEE300 pKa = 4.08FDD302 pKa = 3.02WGFILIADD310 pKa = 4.13EE311 pKa = 4.21

Molecular weight: 33.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A265US35|A0A265US35_9FLAO Alpha/beta hydrolase OS=Winogradskyella aurantia OX=1915063 GN=CA834_10740 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.76GRR40 pKa = 11.84KK41 pKa = 7.91RR42 pKa = 11.84LSVSSEE48 pKa = 3.58ARR50 pKa = 11.84HH51 pKa = 5.78KK52 pKa = 10.72KK53 pKa = 9.83

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2856

0

2856

969902

31

2771

339.6

38.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.403 ± 0.043

0.722 ± 0.012

5.866 ± 0.043

6.504 ± 0.044

5.262 ± 0.035

6.388 ± 0.049

1.803 ± 0.022

7.981 ± 0.04

7.384 ± 0.066

9.465 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.217 ± 0.026

6.019 ± 0.054

3.443 ± 0.026

3.336 ± 0.025

3.672 ± 0.03

6.466 ± 0.034

5.705 ± 0.041

6.23 ± 0.034

1.071 ± 0.017

4.062 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski