Amino acid dipepetide frequency for Flavobacterium aciduliphilum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.03AlaAla: 4.03 ± 0.109	0.635AlaCys: 0.635 ± 0.032	3.013AlaAsp: 3.013 ± 0.055	3.465AlaGlu: 3.465 ± 0.075	3.27AlaPhe: 3.27 ± 0.058	3.844AlaGly: 3.844 ± 0.101	1.207AlaHis: 1.207 ± 0.043	5.215AlaIle: 5.215 ± 0.066	4.648AlaLys: 4.648 ± 0.093	6.48AlaLeu: 6.48 ± 0.113	1.555AlaMet: 1.555 ± 0.047	3.35AlaAsn: 3.35 ± 0.087	2.078AlaPro: 2.078 ± 0.06	2.683AlaGln: 2.683 ± 0.056	1.801AlaArg: 1.801 ± 0.048	4.34AlaSer: 4.34 ± 0.115	4.456AlaThr: 4.456 ± 0.175	3.965AlaVal: 3.965 ± 0.081	0.578AlaTrp: 0.578 ± 0.024	2.329AlaTyr: 2.329 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.543CysAla: 0.543 ± 0.026	0.147CysCys: 0.147 ± 0.014	0.528CysAsp: 0.528 ± 0.033	0.498CysGlu: 0.498 ± 0.028	0.505CysPhe: 0.505 ± 0.025	0.698CysGly: 0.698 ± 0.032	0.233CysHis: 0.233 ± 0.019	0.702CysIle: 0.702 ± 0.027	0.604CysLys: 0.604 ± 0.028	0.713CysLeu: 0.713 ± 0.031	0.171CysMet: 0.171 ± 0.014	0.481CysAsn: 0.481 ± 0.028	0.366CysPro: 0.366 ± 0.026	0.334CysGln: 0.334 ± 0.023	0.229CysArg: 0.229 ± 0.016	0.684CysSer: 0.684 ± 0.035	0.545CysThr: 0.545 ± 0.032	0.555CysVal: 0.555 ± 0.027	0.067CysTrp: 0.067 ± 0.009	0.4CysTyr: 0.4 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	3.651AspAla: 3.651 ± 0.078	0.49AspCys: 0.49 ± 0.025	2.459AspAsp: 2.459 ± 0.063	3.417AspGlu: 3.417 ± 0.082	3.675AspPhe: 3.675 ± 0.071	3.15AspGly: 3.15 ± 0.082	0.852AspHis: 0.852 ± 0.034	3.88AspIle: 3.88 ± 0.069	4.027AspLys: 4.027 ± 0.094	4.77AspLeu: 4.77 ± 0.084	1.081AspMet: 1.081 ± 0.041	2.741AspAsn: 2.741 ± 0.066	1.292AspPro: 1.292 ± 0.041	1.327AspGln: 1.327 ± 0.038	1.651AspArg: 1.651 ± 0.053	2.994AspSer: 2.994 ± 0.062	2.803AspThr: 2.803 ± 0.053	3.562AspVal: 3.562 ± 0.067	0.671AspTrp: 0.671 ± 0.03	2.804AspTyr: 2.804 ± 0.07	0.0AspXaa: 0.0 ± 0.0
Glu	4.188GluAla: 4.188 ± 0.094	0.417GluCys: 0.417 ± 0.024	3.071GluAsp: 3.071 ± 0.072	4.418GluGlu: 4.418 ± 0.097	2.854GluPhe: 2.854 ± 0.064	3.376GluGly: 3.376 ± 0.073	1.323GluHis: 1.323 ± 0.044	5.397GluIle: 5.397 ± 0.097	6.001GluLys: 6.001 ± 0.11	5.929GluLeu: 5.929 ± 0.114	1.535GluMet: 1.535 ± 0.044	4.456GluAsn: 4.456 ± 0.084	1.457GluPro: 1.457 ± 0.05	2.281GluGln: 2.281 ± 0.057	2.336GluArg: 2.336 ± 0.061	3.159GluSer: 3.159 ± 0.062	3.506GluThr: 3.506 ± 0.073	4.097GluVal: 4.097 ± 0.077	0.552GluTrp: 0.552 ± 0.027	2.307GluTyr: 2.307 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	2.905PheAla: 2.905 ± 0.062	0.572PheCys: 0.572 ± 0.029	3.3PheAsp: 3.3 ± 0.069	3.633PheGlu: 3.633 ± 0.07	3.134PhePhe: 3.134 ± 0.088	3.633PheGly: 3.633 ± 0.071	0.992PheHis: 0.992 ± 0.039	4.11PheIle: 4.11 ± 0.092	3.76PheLys: 3.76 ± 0.078	4.933PheLeu: 4.933 ± 0.089	1.182PheMet: 1.182 ± 0.043	3.257PheAsn: 3.257 ± 0.081	1.858PhePro: 1.858 ± 0.052	1.789PheGln: 1.789 ± 0.043	1.593PheArg: 1.593 ± 0.041	4.216PheSer: 4.216 ± 0.087	3.103PheThr: 3.103 ± 0.068	3.417PheVal: 3.417 ± 0.075	0.571PheTrp: 0.571 ± 0.028	2.36PheTyr: 2.36 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.136GlyAla: 4.136 ± 0.111	0.661GlyCys: 0.661 ± 0.042	2.775GlyAsp: 2.775 ± 0.061	2.989GlyGlu: 2.989 ± 0.066	3.613GlyPhe: 3.613 ± 0.076	4.182GlyGly: 4.182 ± 0.094	1.118GlyHis: 1.118 ± 0.041	5.373GlyIle: 5.373 ± 0.086	4.852GlyLys: 4.852 ± 0.087	5.229GlyLeu: 5.229 ± 0.088	1.625GlyMet: 1.625 ± 0.047	3.448GlyAsn: 3.448 ± 0.079	1.281GlyPro: 1.281 ± 0.055	1.881GlyGln: 1.881 ± 0.053	1.84GlyArg: 1.84 ± 0.059	3.842GlySer: 3.842 ± 0.105	4.656GlyThr: 4.656 ± 0.181	4.2GlyVal: 4.2 ± 0.086	0.716GlyTrp: 0.716 ± 0.032	2.801GlyTyr: 2.801 ± 0.069	0.0GlyXaa: 0.0 ± 0.0
His	1.078HisAla: 1.078 ± 0.034	0.224HisCys: 0.224 ± 0.016	0.915HisAsp: 0.915 ± 0.036	1.127HisGlu: 1.127 ± 0.039	1.322HisPhe: 1.322 ± 0.044	1.021HisGly: 1.021 ± 0.039	0.565HisHis: 0.565 ± 0.03	1.415HisIle: 1.415 ± 0.044	1.219HisLys: 1.219 ± 0.041	1.843HisLeu: 1.843 ± 0.053	0.324HisMet: 0.324 ± 0.021	0.992HisAsn: 0.992 ± 0.036	0.871HisPro: 0.871 ± 0.034	0.937HisGln: 0.937 ± 0.037	0.666HisArg: 0.666 ± 0.031	1.107HisSer: 1.107 ± 0.041	1.002HisThr: 1.002 ± 0.04	1.058HisVal: 1.058 ± 0.043	0.211HisTrp: 0.211 ± 0.015	1.018HisTyr: 1.018 ± 0.043	0.0HisXaa: 0.0 ± 0.0
Ile	5.564IleAla: 5.564 ± 0.094	0.722IleCys: 0.722 ± 0.03	4.733IleAsp: 4.733 ± 0.083	5.67IleGlu: 5.67 ± 0.1	3.683IlePhe: 3.683 ± 0.076	5.03IleGly: 5.03 ± 0.091	1.525IleHis: 1.525 ± 0.049	6.022IleIle: 6.022 ± 0.111	6.041IleLys: 6.041 ± 0.091	7.083IleLeu: 7.083 ± 0.113	1.498IleMet: 1.498 ± 0.045	4.845IleAsn: 4.845 ± 0.078	3.386IlePro: 3.386 ± 0.068	3.036IleGln: 3.036 ± 0.067	2.521IleArg: 2.521 ± 0.057	5.556IleSer: 5.556 ± 0.088	5.059IleThr: 5.059 ± 0.102	5.036IleVal: 5.036 ± 0.083	0.705IleTrp: 0.705 ± 0.027	2.929IleTyr: 2.929 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	4.88LysAla: 4.88 ± 0.097	0.448LysCys: 0.448 ± 0.024	4.006LysAsp: 4.006 ± 0.073	6.12LysGlu: 6.12 ± 0.113	3.112LysPhe: 3.112 ± 0.063	4.428LysGly: 4.428 ± 0.078	1.316LysHis: 1.316 ± 0.039	7.158LysIle: 7.158 ± 0.099	7.688LysLys: 7.688 ± 0.121	6.426LysLeu: 6.426 ± 0.098	2.304LysMet: 2.304 ± 0.059	5.88LysAsn: 5.88 ± 0.114	2.558LysPro: 2.558 ± 0.058	2.797LysGln: 2.797 ± 0.065	2.642LysArg: 2.642 ± 0.059	4.694LysSer: 4.694 ± 0.095	4.804LysThr: 4.804 ± 0.08	4.795LysVal: 4.795 ± 0.09	0.757LysTrp: 0.757 ± 0.032	3.197LysTyr: 3.197 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	5.634LeuAla: 5.634 ± 0.099	0.774LeuCys: 0.774 ± 0.03	4.766LeuAsp: 4.766 ± 0.086	5.874LeuGlu: 5.874 ± 0.098	5.391LeuPhe: 5.391 ± 0.121	5.551LeuGly: 5.551 ± 0.091	1.784LeuHis: 1.784 ± 0.056	6.746LeuIle: 6.746 ± 0.115	7.6LeuLys: 7.6 ± 0.109	8.765LeuLeu: 8.765 ± 0.133	2.131LeuMet: 2.131 ± 0.055	5.538LeuAsn: 5.538 ± 0.088	3.313LeuPro: 3.313 ± 0.073	3.339LeuGln: 3.339 ± 0.071	2.794LeuArg: 2.794 ± 0.063	6.414LeuSer: 6.414 ± 0.091	5.145LeuThr: 5.145 ± 0.106	5.369LeuVal: 5.369 ± 0.078	0.755LeuTrp: 0.755 ± 0.031	3.306LeuTyr: 3.306 ± 0.074	0.0LeuXaa: 0.0 ± 0.0
Met	1.568MetAla: 1.568 ± 0.046	0.172MetCys: 0.172 ± 0.014	1.072MetAsp: 1.072 ± 0.039	1.446MetGlu: 1.446 ± 0.049	0.911MetPhe: 0.911 ± 0.036	1.442MetGly: 1.442 ± 0.047	0.396MetHis: 0.396 ± 0.024	1.8MetIle: 1.8 ± 0.048	2.306MetLys: 2.306 ± 0.059	1.862MetLeu: 1.862 ± 0.052	0.657MetMet: 0.657 ± 0.031	1.463MetAsn: 1.463 ± 0.044	0.834MetPro: 0.834 ± 0.039	0.843MetGln: 0.843 ± 0.031	0.863MetArg: 0.863 ± 0.033	1.523MetSer: 1.523 ± 0.044	1.265MetThr: 1.265 ± 0.036	1.389MetVal: 1.389 ± 0.042	0.165MetTrp: 0.165 ± 0.017	0.807MetTyr: 0.807 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	3.836AsnAla: 3.836 ± 0.083	0.548AsnCys: 0.548 ± 0.028	3.248AsnAsp: 3.248 ± 0.066	3.781AsnGlu: 3.781 ± 0.08	3.166AsnPhe: 3.166 ± 0.07	4.032AsnGly: 4.032 ± 0.108	1.201AsnHis: 1.201 ± 0.041	4.639AsnIle: 4.639 ± 0.087	4.503AsnLys: 4.503 ± 0.093	5.271AsnLeu: 5.271 ± 0.086	1.29AsnMet: 1.29 ± 0.04	4.006AsnAsn: 4.006 ± 0.101	3.031AsnPro: 3.031 ± 0.073	2.59AsnGln: 2.59 ± 0.07	2.0AsnArg: 2.0 ± 0.052	4.121AsnSer: 4.121 ± 0.083	3.903AsnThr: 3.903 ± 0.106	3.541AsnVal: 3.541 ± 0.071	0.763AsnTrp: 0.763 ± 0.029	3.069AsnTyr: 3.069 ± 0.079	0.0AsnXaa: 0.0 ± 0.0
Pro	1.906ProAla: 1.906 ± 0.061	0.276ProCys: 0.276 ± 0.02	1.693ProAsp: 1.693 ± 0.047	2.431ProGlu: 2.431 ± 0.061	2.016ProPhe: 2.016 ± 0.05	1.753ProGly: 1.753 ± 0.062	0.607ProHis: 0.607 ± 0.028	3.024ProIle: 3.024 ± 0.057	2.725ProLys: 2.725 ± 0.069	2.87ProLeu: 2.87 ± 0.057	0.799ProMet: 0.799 ± 0.033	2.497ProAsn: 2.497 ± 0.063	0.778ProPro: 0.778 ± 0.034	1.142ProGln: 1.142 ± 0.039	0.878ProArg: 0.878 ± 0.038	2.311ProSer: 2.311 ± 0.061	2.328ProThr: 2.328 ± 0.087	2.209ProVal: 2.209 ± 0.053	0.283ProTrp: 0.283 ± 0.022	1.402ProTyr: 1.402 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	2.178GlnAla: 2.178 ± 0.056	0.253GlnCys: 0.253 ± 0.021	1.654GlnAsp: 1.654 ± 0.054	2.393GlnGlu: 2.393 ± 0.057	1.804GlnPhe: 1.804 ± 0.049	1.817GlnGly: 1.817 ± 0.057	0.683GlnHis: 0.683 ± 0.028	2.844GlnIle: 2.844 ± 0.055	3.305GlnLys: 3.305 ± 0.076	3.452GlnLeu: 3.452 ± 0.07	0.929GlnMet: 0.929 ± 0.033	2.572GlnAsn: 2.572 ± 0.059	1.076GlnPro: 1.076 ± 0.044	1.445GlnGln: 1.445 ± 0.054	1.124GlnArg: 1.124 ± 0.038	2.035GlnSer: 2.035 ± 0.052	2.157GlnThr: 2.157 ± 0.052	2.081GlnVal: 2.081 ± 0.062	0.381GlnTrp: 0.381 ± 0.027	1.534GlnTyr: 1.534 ± 0.046	0.0GlnXaa: 0.0 ± 0.0
Arg	1.896ArgAla: 1.896 ± 0.051	0.233ArgCys: 0.233 ± 0.017	1.531ArgAsp: 1.531 ± 0.053	2.012ArgGlu: 2.012 ± 0.057	1.816ArgPhe: 1.816 ± 0.044	1.7ArgGly: 1.7 ± 0.055	0.58ArgHis: 0.58 ± 0.027	2.825ArgIle: 2.825 ± 0.06	2.665ArgLys: 2.665 ± 0.063	2.916ArgLeu: 2.916 ± 0.061	0.911ArgMet: 0.911 ± 0.038	1.966ArgAsn: 1.966 ± 0.052	0.982ArgPro: 0.982 ± 0.031	0.974ArgGln: 0.974 ± 0.039	1.125ArgArg: 1.125 ± 0.047	1.576ArgSer: 1.576 ± 0.048	1.782ArgThr: 1.782 ± 0.05	1.96ArgVal: 1.96 ± 0.053	0.319ArgTrp: 0.319 ± 0.02	1.4ArgTyr: 1.4 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.605SerAla: 3.605 ± 0.108	0.753SerCys: 0.753 ± 0.03	3.328SerAsp: 3.328 ± 0.06	3.787SerGlu: 3.787 ± 0.075	3.995SerPhe: 3.995 ± 0.076	4.61SerGly: 4.61 ± 0.117	1.165SerHis: 1.165 ± 0.041	5.474SerIle: 5.474 ± 0.085	5.326SerLys: 5.326 ± 0.096	5.699SerLeu: 5.699 ± 0.102	1.34SerMet: 1.34 ± 0.041	4.262SerAsn: 4.262 ± 0.095	2.015SerPro: 2.015 ± 0.059	2.281SerGln: 2.281 ± 0.054	1.894SerArg: 1.894 ± 0.045	4.516SerSer: 4.516 ± 0.112	3.854SerThr: 3.854 ± 0.134	3.997SerVal: 3.997 ± 0.091	0.682SerTrp: 0.682 ± 0.031	2.872SerTyr: 2.872 ± 0.061	0.0SerXaa: 0.0 ± 0.0
Thr	3.94ThrAla: 3.94 ± 0.143	0.498ThrCys: 0.498 ± 0.031	2.951ThrAsp: 2.951 ± 0.072	3.136ThrGlu: 3.136 ± 0.074	3.333ThrPhe: 3.333 ± 0.072	4.012ThrGly: 4.012 ± 0.157	1.115ThrHis: 1.115 ± 0.037	5.53ThrIle: 5.53 ± 0.111	4.298ThrLys: 4.298 ± 0.075	6.059ThrLeu: 6.059 ± 0.102	1.034ThrMet: 1.034 ± 0.035	3.798ThrAsn: 3.798 ± 0.122	2.841ThrPro: 2.841 ± 0.089	2.123ThrGln: 2.123 ± 0.06	1.523ThrArg: 1.523 ± 0.039	4.209ThrSer: 4.209 ± 0.167	4.464ThrThr: 4.464 ± 0.204	3.8ThrVal: 3.8 ± 0.135	0.604ThrTrp: 0.604 ± 0.033	2.669ThrTyr: 2.669 ± 0.098	0.0ThrXaa: 0.0 ± 0.0
Val	4.304ValAla: 4.304 ± 0.084	0.649ValCys: 0.649 ± 0.035	3.354ValAsp: 3.354 ± 0.074	3.606ValGlu: 3.606 ± 0.082	3.522ValPhe: 3.522 ± 0.068	3.786ValGly: 3.786 ± 0.076	1.101ValHis: 1.101 ± 0.04	4.832ValIle: 4.832 ± 0.071	4.526ValLys: 4.526 ± 0.069	5.999ValLeu: 5.999 ± 0.095	1.339ValMet: 1.339 ± 0.048	3.509ValAsn: 3.509 ± 0.072	2.205ValPro: 2.205 ± 0.051	1.868ValGln: 1.868 ± 0.052	1.846ValArg: 1.846 ± 0.052	4.654ValSer: 4.654 ± 0.083	3.969ValThr: 3.969 ± 0.124	4.364ValVal: 4.364 ± 0.078	0.554ValTrp: 0.554 ± 0.026	2.409ValTyr: 2.409 ± 0.064	0.0ValXaa: 0.0 ± 0.0
Trp	0.6TrpAla: 0.6 ± 0.031	0.123TrpCys: 0.123 ± 0.012	0.545TrpAsp: 0.545 ± 0.028	0.559TrpGlu: 0.559 ± 0.027	0.58TrpPhe: 0.58 ± 0.027	0.576TrpGly: 0.576 ± 0.031	0.22TrpHis: 0.22 ± 0.016	0.76TrpIle: 0.76 ± 0.03	0.763TrpLys: 0.763 ± 0.03	0.812TrpLeu: 0.812 ± 0.036	0.318TrpMet: 0.318 ± 0.024	0.79TrpAsn: 0.79 ± 0.033	0.186TrpPro: 0.186 ± 0.017	0.388TrpGln: 0.388 ± 0.024	0.333TrpArg: 0.333 ± 0.023	0.601TrpSer: 0.601 ± 0.037	0.581TrpThr: 0.581 ± 0.031	0.618TrpVal: 0.618 ± 0.03	0.12TrpTrp: 0.12 ± 0.015	0.457TrpTyr: 0.457 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.5TyrAla: 2.5 ± 0.058	0.407TyrCys: 0.407 ± 0.022	2.351TyrAsp: 2.351 ± 0.062	2.299TyrGlu: 2.299 ± 0.063	2.616TyrPhe: 2.616 ± 0.056	2.581TyrGly: 2.581 ± 0.064	0.931TyrHis: 0.931 ± 0.035	2.9TyrIle: 2.9 ± 0.072	3.152TyrLys: 3.152 ± 0.074	3.877TyrLeu: 3.877 ± 0.084	0.76TyrMet: 0.76 ± 0.029	2.692TyrAsn: 2.692 ± 0.063	1.522TyrPro: 1.522 ± 0.047	1.659TyrGln: 1.659 ± 0.057	1.511TyrArg: 1.511 ± 0.048	2.901TyrSer: 2.901 ± 0.066	2.556TyrThr: 2.556 ± 0.094	2.381TyrVal: 2.381 ± 0.053	0.475TyrTrp: 0.475 ± 0.026	2.08TyrTyr: 2.08 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2535 proteins (829962 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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