Ralstonia phage RPSC1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Stompelvirus; Ralstonia virus RPSC1

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2U7X9|A0A2Z2U7X9_9CAUD DNA primase/helicase-like protein OS=Ralstonia phage RPSC1 OX=2041351 GN=RPSC1_32 PE=4 SV=1
MM1 pKa = 7.74AAFDD5 pKa = 4.32WYY7 pKa = 10.58DD8 pKa = 3.42GLPRR12 pKa = 11.84EE13 pKa = 4.59AALAAAGMPEE23 pKa = 5.37RR24 pKa = 11.84VLDD27 pKa = 3.53WDD29 pKa = 4.12TVDD32 pKa = 3.5GEE34 pKa = 4.46EE35 pKa = 4.18WGGKK39 pKa = 8.96HH40 pKa = 6.95DD41 pKa = 4.29CTNANDD47 pKa = 4.53FYY49 pKa = 10.81IDD51 pKa = 3.73PEE53 pKa = 4.75RR54 pKa = 11.84PDD56 pKa = 3.17EE57 pKa = 4.68GYY59 pKa = 9.92WIQTSYY65 pKa = 11.4SYY67 pKa = 11.87DD68 pKa = 3.3NGIQSAYY75 pKa = 10.55SDD77 pKa = 4.61GIRR80 pKa = 11.84LRR82 pKa = 11.84RR83 pKa = 11.84MMVEE87 pKa = 4.02VTKK90 pKa = 10.3MEE92 pKa = 4.4PAYY95 pKa = 10.09EE96 pKa = 3.99ALL98 pKa = 4.03

Molecular weight: 11.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2U7Y3|A0A2Z2U7Y3_9CAUD Exonuclease-like protein OS=Ralstonia phage RPSC1 OX=2041351 GN=RPSC1_37 PE=4 SV=1
MM1 pKa = 7.3NPMIKK6 pKa = 10.13VLTAIHH12 pKa = 6.81RR13 pKa = 11.84APISFQSNYY22 pKa = 9.34ARR24 pKa = 11.84QHH26 pKa = 5.82AGVFAEE32 pKa = 4.2LASRR36 pKa = 11.84GLITCLSTNINTGRR50 pKa = 11.84WMVTPAGLKK59 pKa = 7.8MARR62 pKa = 11.84GEE64 pKa = 4.06

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11839

26

1295

275.3

30.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.411 ± 0.563

0.895 ± 0.177

6.479 ± 0.217

5.609 ± 0.211

3.455 ± 0.173

8.185 ± 0.444

2.103 ± 0.243

4.553 ± 0.181

5.524 ± 0.332

8.007 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.161

3.497 ± 0.163

4.603 ± 0.286

3.818 ± 0.288

5.803 ± 0.221

5.482 ± 0.292

6.039 ± 0.258

6.952 ± 0.258

1.613 ± 0.178

2.982 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski