Haplochromis burtoni (Burton s mouthbrooder) (Chromis burtoni)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia;

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q2W2Y3|A0A3Q2W2Y3_HAPBU Tripartite motif-containing protein 16-like OS=Haplochromis burtoni OX=8153 PE=4 SV=1
MM1 pKa = 7.23PVIWEE6 pKa = 4.22LVSTDD11 pKa = 2.95TNAWSKK17 pKa = 11.54SFGQSLINTFVMYY30 pKa = 10.76LEE32 pKa = 4.8NSEE35 pKa = 4.25QNKK38 pKa = 7.16GTRR41 pKa = 11.84EE42 pKa = 4.23GEE44 pKa = 4.06PSTEE48 pKa = 3.85AMFAEE53 pKa = 4.84QCQVPRR59 pKa = 11.84LTEE62 pKa = 4.04AADD65 pKa = 3.49LTGPSKK71 pKa = 11.0GSAEE75 pKa = 4.24LGVKK79 pKa = 9.95VLLQEE84 pKa = 4.1YY85 pKa = 9.02TEE87 pKa = 4.13QAVSKK92 pKa = 10.25EE93 pKa = 4.13MDD95 pKa = 3.55PSIPCLEE102 pKa = 4.03QCNLNSDD109 pKa = 4.0EE110 pKa = 5.64LEE112 pKa = 3.99SCTYY116 pKa = 10.61FNGHH120 pKa = 6.83ADD122 pKa = 3.14SFEE125 pKa = 4.21QYY127 pKa = 10.43SEE129 pKa = 4.38SNGFFEE135 pKa = 6.0SDD137 pKa = 3.58QILDD141 pKa = 3.47KK142 pKa = 11.57CEE144 pKa = 3.91TSEE147 pKa = 5.08LSQPCDD153 pKa = 3.0EE154 pKa = 4.71CTQHH158 pKa = 6.77CEE160 pKa = 4.02CFKK163 pKa = 11.06SSEE166 pKa = 4.06HH167 pKa = 6.62CGNHH171 pKa = 5.74SSASEE176 pKa = 3.93CSACCEE182 pKa = 3.78QCAEE186 pKa = 4.28HH187 pKa = 6.65LQLFQKK193 pKa = 10.22CKK195 pKa = 10.34PSDD198 pKa = 3.58QQLEE202 pKa = 4.16ISDD205 pKa = 4.46LDD207 pKa = 3.88ADD209 pKa = 4.11EE210 pKa = 6.22LEE212 pKa = 4.71VNCGSLGLCEE222 pKa = 4.7RR223 pKa = 11.84CDD225 pKa = 4.18FNLEE229 pKa = 4.26CTDD232 pKa = 3.76HH233 pKa = 7.43PEE235 pKa = 4.26ALQQYY240 pKa = 8.3EE241 pKa = 4.3PSQQQSDD248 pKa = 4.37CFDD251 pKa = 4.08SEE253 pKa = 5.04SDD255 pKa = 3.16TSTEE259 pKa = 3.88NFQQCEE265 pKa = 4.25TVDD268 pKa = 3.79SLNCNPEE275 pKa = 3.53VCEE278 pKa = 3.77YY279 pKa = 11.4DD280 pKa = 3.88EE281 pKa = 5.92ISDD284 pKa = 3.62EE285 pKa = 4.6TEE287 pKa = 3.79FTNDD291 pKa = 3.27EE292 pKa = 4.21DD293 pKa = 5.13DD294 pKa = 4.36EE295 pKa = 4.34EE296 pKa = 6.33AEE298 pKa = 4.16EE299 pKa = 3.92EE300 pKa = 5.0DD301 pKa = 5.82YY302 pKa = 11.87YY303 pKa = 11.62DD304 pKa = 6.04DD305 pKa = 6.37DD306 pKa = 7.33DD307 pKa = 7.3GDD309 pKa = 4.4FEE311 pKa = 7.75DD312 pKa = 5.23DD313 pKa = 3.66LSYY316 pKa = 10.92KK317 pKa = 10.48AKK319 pKa = 10.45QNEE322 pKa = 4.51TEE324 pKa = 4.23FSDD327 pKa = 4.21EE328 pKa = 4.16EE329 pKa = 4.45SHH331 pKa = 7.3SSSEE335 pKa = 4.07DD336 pKa = 3.31TPVQVFFDD344 pKa = 3.72DD345 pKa = 4.07TEE347 pKa = 4.28NVAFATDD354 pKa = 2.88LWEE357 pKa = 3.89THH359 pKa = 6.43EE360 pKa = 4.12YY361 pKa = 9.38TKK363 pKa = 11.21EE364 pKa = 3.91DD365 pKa = 3.59FQQALTTEE373 pKa = 4.51VPTEE377 pKa = 4.02SGEE380 pKa = 4.07PCGNDD385 pKa = 3.19DD386 pKa = 4.36YY387 pKa = 11.68EE388 pKa = 4.77VSQEE392 pKa = 3.88PAQQYY397 pKa = 7.64STSEE401 pKa = 4.1QYY403 pKa = 10.73KK404 pKa = 9.64SEE406 pKa = 4.18SSGEE410 pKa = 3.88EE411 pKa = 4.31DD412 pKa = 4.12GSSDD416 pKa = 3.65CSSVEE421 pKa = 3.91TKK423 pKa = 10.55SFKK426 pKa = 9.88TCPEE430 pKa = 3.65GSIPSDD436 pKa = 3.43PFSDD440 pKa = 4.01SSEE443 pKa = 4.21EE444 pKa = 4.08SDD446 pKa = 4.85KK447 pKa = 11.47GPQEE451 pKa = 4.46DD452 pKa = 4.29SSDD455 pKa = 3.61EE456 pKa = 3.97QTEE459 pKa = 4.3WEE461 pKa = 4.27SFEE464 pKa = 5.06DD465 pKa = 4.57EE466 pKa = 4.46EE467 pKa = 4.97VQQSDD472 pKa = 3.67EE473 pKa = 4.38NKK475 pKa = 10.4SKK477 pKa = 9.59TVDD480 pKa = 3.44VTVEE484 pKa = 4.23DD485 pKa = 4.37YY486 pKa = 11.32FDD488 pKa = 5.6LFDD491 pKa = 3.83KK492 pKa = 10.82VDD494 pKa = 3.73YY495 pKa = 9.85YY496 pKa = 11.7GHH498 pKa = 7.52IFTQKK503 pKa = 9.46QRR505 pKa = 11.84YY506 pKa = 8.15ISCFDD511 pKa = 3.46GGDD514 pKa = 3.48IHH516 pKa = 7.4DD517 pKa = 4.23HH518 pKa = 6.7LYY520 pKa = 11.08LEE522 pKa = 4.83EE523 pKa = 3.89EE524 pKa = 4.31AKK526 pKa = 10.18KK527 pKa = 10.2HH528 pKa = 5.23KK529 pKa = 10.76AKK531 pKa = 9.68TLYY534 pKa = 10.18QFEE537 pKa = 5.1DD538 pKa = 3.19INRR541 pKa = 11.84EE542 pKa = 4.08ANVKK546 pKa = 8.94DD547 pKa = 3.51TDD549 pKa = 3.85TCSEE553 pKa = 4.31DD554 pKa = 3.63ASEE557 pKa = 4.08DD558 pKa = 3.68TYY560 pKa = 11.9EE561 pKa = 4.4EE562 pKa = 4.28VDD564 pKa = 3.24VDD566 pKa = 4.89QSDD569 pKa = 4.35GSCDD573 pKa = 4.76LEE575 pKa = 4.5TQSEE579 pKa = 4.23DD580 pKa = 2.83WLIAKK585 pKa = 9.83SEE587 pKa = 4.34SSFEE591 pKa = 4.05EE592 pKa = 4.29DD593 pKa = 5.07DD594 pKa = 3.91EE595 pKa = 5.45SKK597 pKa = 10.06TEE599 pKa = 3.91NGVVYY604 pKa = 10.82AEE606 pKa = 4.07NDD608 pKa = 3.08EE609 pKa = 4.22TAEE612 pKa = 4.29DD613 pKa = 3.77DD614 pKa = 3.84KK615 pKa = 12.04APIDD619 pKa = 4.01EE620 pKa = 4.58EE621 pKa = 4.95ACASDD626 pKa = 3.53DD627 pKa = 3.95HH628 pKa = 8.44VSEE631 pKa = 4.28EE632 pKa = 4.52AEE634 pKa = 4.25VRR636 pKa = 11.84PSTGPEE642 pKa = 3.5GSMFAPLAKK651 pKa = 9.86EE652 pKa = 3.72ISVEE656 pKa = 3.59GDD658 pKa = 2.9AYY660 pKa = 10.61EE661 pKa = 6.01DD662 pKa = 3.72ILCSSHH668 pKa = 6.71YY669 pKa = 10.5CEE671 pKa = 5.27PKK673 pKa = 10.4PSATDD678 pKa = 3.32EE679 pKa = 4.27TASITDD685 pKa = 3.39YY686 pKa = 11.59TEE688 pKa = 4.17DD689 pKa = 4.19DD690 pKa = 3.62KK691 pKa = 11.98AKK693 pKa = 10.36PEE695 pKa = 4.34PEE697 pKa = 4.2DD698 pKa = 4.52DD699 pKa = 4.17VFITCSEE706 pKa = 4.16KK707 pKa = 10.35EE708 pKa = 4.48PYY710 pKa = 9.08WSLIDD715 pKa = 3.76NDD717 pKa = 4.22EE718 pKa = 3.85NRR720 pKa = 11.84QLCEE724 pKa = 4.1PGVEE728 pKa = 4.08EE729 pKa = 5.11YY730 pKa = 10.9YY731 pKa = 10.88VYY733 pKa = 10.38QIKK736 pKa = 10.5SIQSSFKK743 pKa = 9.49QALNGFILEE752 pKa = 4.4RR753 pKa = 11.84NSHH756 pKa = 4.69NQITNDD762 pKa = 3.35EE763 pKa = 4.19NASRR767 pKa = 11.84SQSQDD772 pKa = 2.59ALISLKK778 pKa = 10.4EE779 pKa = 3.75LAATGLCPEE788 pKa = 4.66EE789 pKa = 4.57CVSDD793 pKa = 3.43TFGINDD799 pKa = 4.1VIDD802 pKa = 3.93MNSSAVEE809 pKa = 4.09EE810 pKa = 4.22EE811 pKa = 4.49CVLNEE816 pKa = 3.97ISRR819 pKa = 11.84EE820 pKa = 3.95TGPPVGIIHH829 pKa = 6.49SVQEE833 pKa = 3.84RR834 pKa = 11.84DD835 pKa = 3.35MVLMKK840 pKa = 10.74TEE842 pKa = 4.82DD843 pKa = 3.77NNDD846 pKa = 3.48SEE848 pKa = 4.58QSRR851 pKa = 11.84EE852 pKa = 3.97SDD854 pKa = 3.12EE855 pKa = 4.82DD856 pKa = 3.63QSDD859 pKa = 3.83DD860 pKa = 3.76EE861 pKa = 5.11SYY863 pKa = 8.0EE864 pKa = 4.06TCEE867 pKa = 4.22CDD869 pKa = 3.49YY870 pKa = 11.01CITPTEE876 pKa = 4.13EE877 pKa = 4.62LPGEE881 pKa = 4.06PLLPQMEE888 pKa = 4.81SNDD891 pKa = 3.5EE892 pKa = 3.98GKK894 pKa = 9.71ICVVIDD900 pKa = 3.71LDD902 pKa = 3.74EE903 pKa = 4.47TLVHH907 pKa = 7.06SSFKK911 pKa = 10.36PVNNADD917 pKa = 4.05FIIPVEE923 pKa = 3.92IDD925 pKa = 3.4GTVHH929 pKa = 5.33QVYY932 pKa = 9.86VLKK935 pKa = 10.52RR936 pKa = 11.84PHH938 pKa = 7.15VDD940 pKa = 2.95EE941 pKa = 4.17FLKK944 pKa = 10.97RR945 pKa = 11.84MGEE948 pKa = 3.93MFEE951 pKa = 4.9CVLFTASLSKK961 pKa = 11.06YY962 pKa = 9.64ADD964 pKa = 3.7PVSDD968 pKa = 6.39LLDD971 pKa = 2.94KK972 pKa = 10.75WGAFRR977 pKa = 11.84SRR979 pKa = 11.84LFRR982 pKa = 11.84EE983 pKa = 3.88ACVFHH988 pKa = 6.91KK989 pKa = 10.84GNYY992 pKa = 9.11VKK994 pKa = 10.65DD995 pKa = 3.95LSRR998 pKa = 11.84LGRR1001 pKa = 11.84DD1002 pKa = 3.3LNKK1005 pKa = 10.22VIILDD1010 pKa = 3.92NSPASYY1016 pKa = 10.24IFHH1019 pKa = 7.73PEE1021 pKa = 3.29NAVPVASWFNDD1032 pKa = 3.14MSDD1035 pKa = 3.64TEE1037 pKa = 4.74LLDD1040 pKa = 4.78LIPFFEE1046 pKa = 4.75RR1047 pKa = 11.84LSKK1050 pKa = 10.91VDD1052 pKa = 4.9DD1053 pKa = 4.23IYY1055 pKa = 11.42DD1056 pKa = 3.65ILKK1059 pKa = 9.52HH1060 pKa = 5.91QSTSQSS1066 pKa = 3.06

Molecular weight: 120.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q2WAV9|A0A3Q2WAV9_HAPBU PHD finger protein 23 OS=Haplochromis burtoni OX=8153 PE=4 SV=1
WW1 pKa = 7.07GLSPEE6 pKa = 4.09KK7 pKa = 10.27SSRR10 pKa = 11.84PFARR14 pKa = 11.84LRR16 pKa = 11.84EE17 pKa = 4.06PNRR20 pKa = 11.84IQEE23 pKa = 4.26AALFVPQPVRR33 pKa = 11.84SQILQWAHH41 pKa = 5.04TARR44 pKa = 11.84FSCHH48 pKa = 6.12PGIHH52 pKa = 5.45RR53 pKa = 11.84TITFLQRR60 pKa = 11.84YY61 pKa = 7.1FWWPTLSRR69 pKa = 11.84DD70 pKa = 3.26AKK72 pKa = 10.04EE73 pKa = 4.01YY74 pKa = 11.13VSACTTCARR83 pKa = 11.84NKK85 pKa = 10.05IPNQPPSGSLHH96 pKa = 6.57PLPTPTRR103 pKa = 11.84PWSHH107 pKa = 5.83VSLDD111 pKa = 3.96FVTGLPPSAGNTVILTIIDD130 pKa = 3.92RR131 pKa = 11.84FSKK134 pKa = 10.35AAHH137 pKa = 5.77FVALPKK143 pKa = 10.68LPTALEE149 pKa = 4.09TARR152 pKa = 11.84LLTTHH157 pKa = 6.2VFRR160 pKa = 11.84LHH162 pKa = 7.38GIPDD166 pKa = 5.07DD167 pKa = 3.73IVSDD171 pKa = 4.23RR172 pKa = 11.84GPQFTSRR179 pKa = 11.84VWRR182 pKa = 11.84EE183 pKa = 3.45FCTSLGSKK191 pKa = 10.28VSLSSGYY198 pKa = 10.08HH199 pKa = 5.1PQSNGQSEE207 pKa = 4.5RR208 pKa = 11.84VNQEE212 pKa = 3.15LEE214 pKa = 3.68AALRR218 pKa = 11.84CVVSSNQAVWSEE230 pKa = 3.87EE231 pKa = 4.15LPWIEE236 pKa = 3.99YY237 pKa = 9.84AHH239 pKa = 6.68NSMTSSATGRR249 pKa = 11.84SPFEE253 pKa = 4.14ASLGFQPPLFPSIEE267 pKa = 4.32GEE269 pKa = 3.9HH270 pKa = 6.15SVPSVRR276 pKa = 11.84AHH278 pKa = 6.45LRR280 pKa = 11.84RR281 pKa = 11.84CRR283 pKa = 11.84RR284 pKa = 11.84VWKK287 pKa = 9.0ATRR290 pKa = 11.84AAFLRR295 pKa = 11.84TKK297 pKa = 10.55DD298 pKa = 3.37RR299 pKa = 11.84NKK301 pKa = 10.28RR302 pKa = 11.84FADD305 pKa = 3.8RR306 pKa = 11.84RR307 pKa = 11.84RR308 pKa = 11.84SSAPAYY314 pKa = 9.29TVGQKK319 pKa = 9.12VWLSTRR325 pKa = 11.84FVPIRR330 pKa = 11.84AEE332 pKa = 4.01SRR334 pKa = 11.84KK335 pKa = 10.02LSPAYY340 pKa = 9.42IGPFEE345 pKa = 4.67ISALVNN351 pKa = 3.57

Molecular weight: 39.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23059

11273

34332

19812588

17

8592

577.1

64.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.475 ± 0.01

2.23 ± 0.009

5.155 ± 0.009

6.863 ± 0.018

3.644 ± 0.01

6.238 ± 0.015

2.658 ± 0.007

4.468 ± 0.009

5.747 ± 0.012

9.466 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.364 ± 0.005

3.91 ± 0.007

5.716 ± 0.016

4.741 ± 0.011

5.555 ± 0.012

8.843 ± 0.017

5.696 ± 0.009

6.276 ± 0.01

1.138 ± 0.005

2.775 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski