Gordonia phage WilliamBoone

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Smoothievirus; unclassified Smoothievirus

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410TBV9|A0A410TBV9_9CAUD Uncharacterized protein OS=Gordonia phage WilliamBoone OX=2507857 GN=38 PE=4 SV=1
MM1 pKa = 7.07NGSWVGAGYY10 pKa = 10.43QLVGPYY16 pKa = 9.77RR17 pKa = 11.84PAGGDD22 pKa = 3.19LTGGVTKK29 pKa = 10.35VQIMTRR35 pKa = 11.84DD36 pKa = 3.42PVLEE40 pKa = 4.04YY41 pKa = 11.09LDD43 pKa = 4.47NFPGAPLSLWTEE55 pKa = 3.77QGIYY59 pKa = 10.66DD60 pKa = 4.15CVGVQQDD67 pKa = 3.63WFPGGGDD74 pKa = 3.16VWFCDD79 pKa = 2.91TWLEE83 pKa = 4.3LKK85 pKa = 10.77ARR87 pKa = 11.84TT88 pKa = 3.99

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410TCB8|A0A410TCB8_9CAUD Uncharacterized protein OS=Gordonia phage WilliamBoone OX=2507857 GN=43 PE=4 SV=1
MM1 pKa = 7.69SDD3 pKa = 4.05SIVAAADD10 pKa = 3.03SARR13 pKa = 11.84RR14 pKa = 11.84VRR16 pKa = 11.84RR17 pKa = 11.84LVLPGSRR24 pKa = 11.84AWAVIALFTLYY35 pKa = 10.68HH36 pKa = 6.36EE37 pKa = 4.71LRR39 pKa = 11.84CAEE42 pKa = 4.28GEE44 pKa = 4.22LLSEE48 pKa = 4.31AVDD51 pKa = 3.62RR52 pKa = 11.84AIIRR56 pKa = 11.84HH57 pKa = 5.44PVLTRR62 pKa = 11.84LLFAVIMLHH71 pKa = 6.24LNNWIPNKK79 pKa = 10.46LLFLDD84 pKa = 4.92PFHH87 pKa = 6.37QAAVLTKK94 pKa = 10.68NLVARR99 pKa = 4.83

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

185

0

185

29138

16

2931

157.5

17.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.469 ± 0.326

1.091 ± 0.151

6.974 ± 0.182

7.025 ± 0.195

3.085 ± 0.105

7.828 ± 0.23

2.354 ± 0.175

4.853 ± 0.134

4.139 ± 0.163

7.677 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.107

3.672 ± 0.176

4.853 ± 0.141

3.59 ± 0.223

6.984 ± 0.203

5.797 ± 0.141

5.786 ± 0.194

7.056 ± 0.191

2.272 ± 0.123

3.013 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski