Streptococcus satellite phage Javan402
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZMP4|A0A4D5ZMP4_9VIRU Integrase OS=Streptococcus satellite phage Javan402 OX=2558681 GN=JavanS402_0001 PE=3 SV=1
MM1 pKa = 7.2 KK2 pKa = 9.64 TLSDD6 pKa = 3.59 TPKK9 pKa = 9.76 TFTFTTTLPNFEE21 pKa = 4.31 QAQKK25 pKa = 10.83 AGSALIGYY33 pKa = 7.54 MVGTYY38 pKa = 9.42 DD39 pKa = 4.57 QSAIDD44 pKa = 3.19 ITYY47 pKa = 9.93 IGNGDD52 pKa = 3.54 IEE54 pKa = 4.52 VEE56 pKa = 4.29 YY57 pKa = 10.52 TSDD60 pKa = 3.79 EE61 pKa = 4.26 DD62 pKa = 3.41 LTEE65 pKa = 4.05 NFEE68 pKa = 4.83 RR69 pKa = 11.84 IANSLDD75 pKa = 3.33 HH76 pKa = 7.05 TDD78 pKa = 4.04 DD79 pKa = 4.3 FEE81 pKa = 7.25 DD82 pKa = 4.38 YY83 pKa = 11.27 EE84 pKa = 4.49 EE85 pKa = 5.88 DD86 pKa = 3.84 EE87 pKa = 4.46 PTLKK91 pKa = 10.85 GDD93 pKa = 3.89 LDD95 pKa = 4.15 TYY97 pKa = 9.8 TALIGSFDD105 pKa = 3.91 TLGQAQAFTEE115 pKa = 4.03 NLDD118 pKa = 4.42 DD119 pKa = 5.64 NLTNGNNFVFEE130 pKa = 4.15 QRR132 pKa = 11.84 PDD134 pKa = 3.44 TVVVYY139 pKa = 8.67 VSPQYY144 pKa = 10.65 TIANSTIQKK153 pKa = 10.38 LEE155 pKa = 3.57 EE156 pKa = 4.35 AYY158 pKa = 11.02 VKK160 pKa = 10.6 FEE162 pKa = 4.6 ADD164 pKa = 3.59 YY165 pKa = 11.33 EE166 pKa = 4.41 PDD168 pKa = 3.43 TFRR171 pKa = 11.84 LSYY174 pKa = 10.96 HH175 pKa = 6.43 DD176 pKa = 4.11 MRR178 pKa = 11.84 VQQLQSLDD186 pKa = 3.52 KK187 pKa = 10.91 DD188 pKa = 3.39 ILINDD193 pKa = 4.29 IIAYY197 pKa = 9.12 EE198 pKa = 4.44 LEE200 pKa = 4.52 LLDD203 pKa = 4.44 HH204 pKa = 7.54 ADD206 pKa = 3.89 RR207 pKa = 11.84 LLSDD211 pKa = 4.22 EE212 pKa = 5.14 PLPLDD217 pKa = 4.17 DD218 pKa = 3.37 QHH220 pKa = 8.21 GFEE223 pKa = 4.67 TIEE226 pKa = 4.04 LLGDD230 pKa = 3.74 DD231 pKa = 4.17 VIKK234 pKa = 10.57 LVKK237 pKa = 10.4 EE238 pKa = 3.67 LDD240 pKa = 3.71 TNKK243 pKa = 10.24 EE244 pKa = 3.38 LDD246 pKa = 4.4 GIHH249 pKa = 7.43 DD250 pKa = 4.37 YY251 pKa = 11.02 IIGGG255 pKa = 3.65
Molecular weight: 28.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.976
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.935
Patrickios 2.943
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|A0A4D5ZMQ5|A0A4D5ZMQ5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan402 OX=2558681 GN=JavanS402_0011 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.2 YY6 pKa = 10.55 KK7 pKa = 10.52 KK8 pKa = 10.31 KK9 pKa = 10.41 DD10 pKa = 3.25 GTIMYY15 pKa = 9.49 RR16 pKa = 11.84 SQIYY20 pKa = 10.55 LGVDD24 pKa = 2.94 SVTGKK29 pKa = 10.43 KK30 pKa = 10.34 ARR32 pKa = 11.84 TSVSGRR38 pKa = 11.84 TKK40 pKa = 10.78 KK41 pKa = 9.89 EE42 pKa = 3.5 LKK44 pKa = 10.87 NNIKK48 pKa = 9.59 LAKK51 pKa = 10.46 YY52 pKa = 8.03 EE53 pKa = 4.07 FQANGEE59 pKa = 4.46 TVSKK63 pKa = 10.21 KK64 pKa = 9.68 VQVKK68 pKa = 8.92 TYY70 pKa = 9.61 SQLVEE75 pKa = 4.2 LWLEE79 pKa = 4.11 NYY81 pKa = 10.23 KK82 pKa = 9.32 LTIKK86 pKa = 9.75 PQTYY90 pKa = 9.3 RR91 pKa = 11.84 VTTRR95 pKa = 11.84 LIDD98 pKa = 3.71 CHH100 pKa = 6.68 LLPYY104 pKa = 9.22 FGKK107 pKa = 9.76 MKK109 pKa = 10.46 VEE111 pKa = 4.78 KK112 pKa = 10.36 ISNTDD117 pKa = 2.49 IQSFVNGLSSKK128 pKa = 10.17 LVNFRR133 pKa = 11.84 TVNSINTRR141 pKa = 11.84 VLQYY145 pKa = 10.84 GVSLNLIPFNPARR158 pKa = 11.84 DD159 pKa = 3.53 VILPKK164 pKa = 9.99 KK165 pKa = 9.8 QKK167 pKa = 10.5 AGRR170 pKa = 11.84 DD171 pKa = 3.44 SVKK174 pKa = 10.83 FIDD177 pKa = 3.97 TNDD180 pKa = 3.44 LKK182 pKa = 10.45 TLLNFMEE189 pKa = 4.37 KK190 pKa = 10.19 QSFRR194 pKa = 11.84 RR195 pKa = 11.84 YY196 pKa = 8.64 RR197 pKa = 11.84 YY198 pKa = 7.54 QVEE201 pKa = 4.13 YY202 pKa = 11.1 VFYY205 pKa = 10.82 KK206 pKa = 10.89 LLLATGLRR214 pKa = 11.84 IGEE217 pKa = 4.13 AVALEE222 pKa = 4.13 WSDD225 pKa = 3.65 IDD227 pKa = 5.53 LEE229 pKa = 4.32 NATIDD234 pKa = 3.45 INKK237 pKa = 9.66 SFSQEE242 pKa = 3.49 LRR244 pKa = 11.84 LVSTTKK250 pKa = 10.14 TKK252 pKa = 10.93 AGTRR256 pKa = 11.84 LISIDD261 pKa = 3.8 KK262 pKa = 8.85 EE263 pKa = 4.68 TVNLLKK269 pKa = 10.53 LYY271 pKa = 8.46 QVRR274 pKa = 11.84 QRR276 pKa = 11.84 QLFHH280 pKa = 6.65 EE281 pKa = 4.75 VGGGVPKK288 pKa = 10.71 VAFATPTRR296 pKa = 11.84 EE297 pKa = 3.87 YY298 pKa = 10.93 LSRR301 pKa = 11.84 LTLQNNLNSRR311 pKa = 11.84 CEE313 pKa = 3.75 RR314 pKa = 11.84 LGIPRR319 pKa = 11.84 FTFHH323 pKa = 7.74 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.52 RR346 pKa = 11.84 LGHH349 pKa = 5.94 SNIAMTLDD357 pKa = 3.93 VYY359 pKa = 11.5 SHH361 pKa = 7.06 LSKK364 pKa = 11.04 DD365 pKa = 3.51 KK366 pKa = 10.41 EE367 pKa = 4.25 KK368 pKa = 10.93 EE369 pKa = 3.85 AVSYY373 pKa = 9.5 YY374 pKa = 10.51 EE375 pKa = 4.18 KK376 pKa = 10.61 AINSII381 pKa = 3.83
Molecular weight: 43.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.11
IPC2_protein 9.341
IPC_protein 9.282
Toseland 9.984
ProMoST 9.663
Dawson 10.189
Bjellqvist 9.853
Wikipedia 10.35
Rodwell 10.672
Grimsley 10.262
Solomon 10.204
Lehninger 10.175
Nozaki 9.97
DTASelect 9.853
Thurlkill 10.043
EMBOSS 10.394
Sillero 10.101
Patrickios 10.131
IPC_peptide 10.204
IPC2_peptide 8.375
IPC2.peptide.svr19 8.335
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
3048
44
415
145.1
16.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.921 ± 0.533
0.623 ± 0.134
6.365 ± 0.738
6.988 ± 0.67
3.806 ± 0.214
4.298 ± 0.457
1.345 ± 0.159
8.53 ± 0.74
9.613 ± 0.955
11.286 ± 0.6
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.592 ± 0.295
6.496 ± 0.42
3.117 ± 0.529
4.003 ± 0.319
4.101 ± 0.464
5.249 ± 0.57
6.693 ± 0.557
4.298 ± 0.538
0.656 ± 0.151
5.02 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here