Amino acid dipepetide frequency for Proteus phage Saba

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.831AlaAla: 7.831 ± 1.249
0.635AlaCys: 0.635 ± 0.203
4.868AlaAsp: 4.868 ± 0.526
7.514AlaGlu: 7.514 ± 1.044
3.069AlaPhe: 3.069 ± 0.365
6.244AlaGly: 6.244 ± 0.73
1.164AlaHis: 1.164 ± 0.186
4.074AlaIle: 4.074 ± 0.518
6.932AlaLys: 6.932 ± 1.421
7.619AlaLeu: 7.619 ± 0.754
2.275AlaMet: 2.275 ± 0.555
2.857AlaAsn: 2.857 ± 0.545
3.175AlaPro: 3.175 ± 0.451
3.069AlaGln: 3.069 ± 0.455
4.656AlaArg: 4.656 ± 0.457
5.238AlaSer: 5.238 ± 0.526
4.021AlaThr: 4.021 ± 0.337
6.667AlaVal: 6.667 ± 0.705
1.376AlaTrp: 1.376 ± 0.246
3.069AlaTyr: 3.069 ± 0.358
0.0AlaXaa: 0.0 ± 0.0
Cys
0.582CysAla: 0.582 ± 0.162
0.0CysCys: 0.0 ± 0.0
0.688CysAsp: 0.688 ± 0.187
0.9CysGlu: 0.9 ± 0.221
0.529CysPhe: 0.529 ± 0.157
1.058CysGly: 1.058 ± 0.291
0.159CysHis: 0.159 ± 0.091
0.476CysIle: 0.476 ± 0.154
0.423CysLys: 0.423 ± 0.14
0.635CysLeu: 0.635 ± 0.164
0.317CysMet: 0.317 ± 0.128
0.317CysAsn: 0.317 ± 0.142
0.741CysPro: 0.741 ± 0.24
0.317CysGln: 0.317 ± 0.13
0.582CysArg: 0.582 ± 0.184
0.794CysSer: 0.794 ± 0.228
0.317CysThr: 0.317 ± 0.144
0.847CysVal: 0.847 ± 0.186
0.106CysTrp: 0.106 ± 0.081
0.529CysTyr: 0.529 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
5.238AspAla: 5.238 ± 0.535
0.582AspCys: 0.582 ± 0.178
4.498AspAsp: 4.498 ± 0.677
4.498AspGlu: 4.498 ± 0.575
2.434AspPhe: 2.434 ± 0.305
6.085AspGly: 6.085 ± 0.579
1.323AspHis: 1.323 ± 0.213
4.18AspIle: 4.18 ± 0.484
3.81AspLys: 3.81 ± 0.411
4.974AspLeu: 4.974 ± 0.473
1.693AspMet: 1.693 ± 0.335
3.281AspAsn: 3.281 ± 0.365
2.963AspPro: 2.963 ± 0.436
1.534AspGln: 1.534 ± 0.226
3.598AspArg: 3.598 ± 0.371
2.963AspSer: 2.963 ± 0.325
3.386AspThr: 3.386 ± 0.454
4.603AspVal: 4.603 ± 0.485
1.64AspTrp: 1.64 ± 0.383
2.011AspTyr: 2.011 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
5.873GluAla: 5.873 ± 0.75
0.476GluCys: 0.476 ± 0.17
4.656GluAsp: 4.656 ± 0.554
4.868GluGlu: 4.868 ± 0.516
2.646GluPhe: 2.646 ± 0.349
3.175GluGly: 3.175 ± 0.347
0.9GluHis: 0.9 ± 0.222
4.815GluIle: 4.815 ± 0.514
4.074GluLys: 4.074 ± 0.481
4.603GluLeu: 4.603 ± 0.562
2.804GluMet: 2.804 ± 0.377
3.228GluAsn: 3.228 ± 0.503
2.169GluPro: 2.169 ± 0.47
2.434GluGln: 2.434 ± 0.413
3.386GluArg: 3.386 ± 0.432
5.238GluSer: 5.238 ± 0.5
3.598GluThr: 3.598 ± 0.39
5.291GluVal: 5.291 ± 0.509
1.217GluTrp: 1.217 ± 0.194
1.905GluTyr: 1.905 ± 0.313
0.0GluXaa: 0.0 ± 0.0
Phe
3.439PheAla: 3.439 ± 0.429
0.582PheCys: 0.582 ± 0.172
3.016PheAsp: 3.016 ± 0.425
3.334PheGlu: 3.334 ± 0.282
1.587PhePhe: 1.587 ± 0.295
3.016PheGly: 3.016 ± 0.321
0.423PheHis: 0.423 ± 0.178
2.646PheIle: 2.646 ± 0.375
2.963PheLys: 2.963 ± 0.575
2.804PheLeu: 2.804 ± 0.399
1.058PheMet: 1.058 ± 0.237
2.064PheAsn: 2.064 ± 0.299
1.905PhePro: 1.905 ± 0.301
0.635PheGln: 0.635 ± 0.172
1.746PheArg: 1.746 ± 0.284
2.275PheSer: 2.275 ± 0.358
3.228PheThr: 3.228 ± 0.528
2.328PheVal: 2.328 ± 0.335
0.317PheTrp: 0.317 ± 0.126
1.164PheTyr: 1.164 ± 0.258
0.0PheXaa: 0.0 ± 0.0
Gly
5.027GlyAla: 5.027 ± 0.583
0.794GlyCys: 0.794 ± 0.268
4.709GlyAsp: 4.709 ± 0.556
5.45GlyGlu: 5.45 ± 0.457
3.122GlyPhe: 3.122 ± 0.427
5.503GlyGly: 5.503 ± 0.734
1.323GlyHis: 1.323 ± 0.283
4.286GlyIle: 4.286 ± 0.465
5.715GlyLys: 5.715 ± 0.596
5.133GlyLeu: 5.133 ± 0.515
1.799GlyMet: 1.799 ± 0.297
2.804GlyAsn: 2.804 ± 0.293
1.429GlyPro: 1.429 ± 0.238
2.328GlyGln: 2.328 ± 0.391
4.656GlyArg: 4.656 ± 0.392
4.339GlySer: 4.339 ± 0.521
3.069GlyThr: 3.069 ± 0.326
6.032GlyVal: 6.032 ± 0.533
1.376GlyTrp: 1.376 ± 0.27
2.91GlyTyr: 2.91 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
1.323HisAla: 1.323 ± 0.284
0.159HisCys: 0.159 ± 0.086
0.847HisAsp: 0.847 ± 0.225
0.9HisGlu: 0.9 ± 0.203
0.529HisPhe: 0.529 ± 0.144
1.005HisGly: 1.005 ± 0.198
0.212HisHis: 0.212 ± 0.124
1.64HisIle: 1.64 ± 0.344
0.794HisLys: 0.794 ± 0.222
1.534HisLeu: 1.534 ± 0.282
0.212HisMet: 0.212 ± 0.125
0.529HisAsn: 0.529 ± 0.136
0.688HisPro: 0.688 ± 0.187
0.265HisGln: 0.265 ± 0.105
1.005HisArg: 1.005 ± 0.233
1.429HisSer: 1.429 ± 0.247
0.741HisThr: 0.741 ± 0.198
1.111HisVal: 1.111 ± 0.262
0.053HisTrp: 0.053 ± 0.055
0.688HisTyr: 0.688 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
5.133IleAla: 5.133 ± 0.421
0.582IleCys: 0.582 ± 0.137
4.921IleAsp: 4.921 ± 0.434
3.916IleGlu: 3.916 ± 0.43
2.117IlePhe: 2.117 ± 0.393
3.386IleGly: 3.386 ± 0.364
1.164IleHis: 1.164 ± 0.311
3.863IleIle: 3.863 ± 0.479
3.863IleLys: 3.863 ± 0.453
3.492IleLeu: 3.492 ± 0.397
1.27IleMet: 1.27 ± 0.253
3.016IleAsn: 3.016 ± 0.388
2.857IlePro: 2.857 ± 0.356
1.799IleGln: 1.799 ± 0.265
3.916IleArg: 3.916 ± 0.507
3.386IleSer: 3.386 ± 0.391
4.021IleThr: 4.021 ± 0.516
4.233IleVal: 4.233 ± 0.376
0.37IleTrp: 0.37 ± 0.139
2.646IleTyr: 2.646 ± 0.337
0.0IleXaa: 0.0 ± 0.0
Lys
6.561LysAla: 6.561 ± 1.119
0.635LysCys: 0.635 ± 0.176
4.445LysAsp: 4.445 ± 0.537
3.81LysGlu: 3.81 ± 0.441
2.804LysPhe: 2.804 ± 0.306
4.021LysGly: 4.021 ± 0.405
1.111LysHis: 1.111 ± 0.23
3.757LysIle: 3.757 ± 0.47
4.233LysLys: 4.233 ± 0.581
4.603LysLeu: 4.603 ± 0.481
2.381LysMet: 2.381 ± 0.415
2.222LysAsn: 2.222 ± 0.33
4.233LysPro: 4.233 ± 0.517
1.746LysGln: 1.746 ± 0.351
3.334LysArg: 3.334 ± 0.409
4.286LysSer: 4.286 ± 0.379
4.127LysThr: 4.127 ± 0.459
4.815LysVal: 4.815 ± 0.517
0.741LysTrp: 0.741 ± 0.198
1.746LysTyr: 1.746 ± 0.355
0.0LysXaa: 0.0 ± 0.0
Leu
6.402LeuAla: 6.402 ± 0.812
1.005LeuCys: 1.005 ± 0.243
3.968LeuAsp: 3.968 ± 0.44
4.921LeuGlu: 4.921 ± 0.419
2.751LeuPhe: 2.751 ± 0.35
5.344LeuGly: 5.344 ± 0.602
0.635LeuHis: 0.635 ± 0.161
3.386LeuIle: 3.386 ± 0.369
6.932LeuLys: 6.932 ± 0.66
4.339LeuLeu: 4.339 ± 0.42
2.328LeuMet: 2.328 ± 0.329
3.439LeuAsn: 3.439 ± 0.4
2.963LeuPro: 2.963 ± 0.49
2.169LeuGln: 2.169 ± 0.301
4.18LeuArg: 4.18 ± 0.501
5.662LeuSer: 5.662 ± 0.631
5.556LeuThr: 5.556 ± 0.515
5.291LeuVal: 5.291 ± 0.551
0.688LeuTrp: 0.688 ± 0.167
2.381LeuTyr: 2.381 ± 0.299
0.0LeuXaa: 0.0 ± 0.0
Met
3.439MetAla: 3.439 ± 0.55
0.529MetCys: 0.529 ± 0.184
1.164MetAsp: 1.164 ± 0.24
1.376MetGlu: 1.376 ± 0.238
1.058MetPhe: 1.058 ± 0.168
1.64MetGly: 1.64 ± 0.245
0.265MetHis: 0.265 ± 0.099
2.117MetIle: 2.117 ± 0.274
1.164MetLys: 1.164 ± 0.313
2.275MetLeu: 2.275 ± 0.322
0.635MetMet: 0.635 ± 0.171
1.164MetAsn: 1.164 ± 0.268
1.058MetPro: 1.058 ± 0.239
0.529MetGln: 0.529 ± 0.204
1.587MetArg: 1.587 ± 0.236
2.751MetSer: 2.751 ± 0.415
1.429MetThr: 1.429 ± 0.24
1.852MetVal: 1.852 ± 0.321
0.159MetTrp: 0.159 ± 0.08
0.741MetTyr: 0.741 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
3.81AsnAla: 3.81 ± 0.795
0.37AsnCys: 0.37 ± 0.158
2.434AsnAsp: 2.434 ± 0.349
1.905AsnGlu: 1.905 ± 0.261
1.587AsnPhe: 1.587 ± 0.28
3.968AsnGly: 3.968 ± 0.42
0.476AsnHis: 0.476 ± 0.19
2.381AsnIle: 2.381 ± 0.343
3.016AsnLys: 3.016 ± 0.372
3.281AsnLeu: 3.281 ± 0.422
0.741AsnMet: 0.741 ± 0.234
1.852AsnAsn: 1.852 ± 0.367
2.54AsnPro: 2.54 ± 0.443
1.058AsnGln: 1.058 ± 0.283
2.222AsnArg: 2.222 ± 0.422
2.169AsnSer: 2.169 ± 0.369
2.751AsnThr: 2.751 ± 0.373
3.545AsnVal: 3.545 ± 0.435
0.423AsnTrp: 0.423 ± 0.123
1.429AsnTyr: 1.429 ± 0.254
0.0AsnXaa: 0.0 ± 0.0
Pro
2.91ProAla: 2.91 ± 0.441
0.582ProCys: 0.582 ± 0.197
3.334ProAsp: 3.334 ± 0.533
2.699ProGlu: 2.699 ± 0.356
1.587ProPhe: 1.587 ± 0.316
4.021ProGly: 4.021 ± 0.479
0.688ProHis: 0.688 ± 0.216
2.751ProIle: 2.751 ± 0.434
2.222ProLys: 2.222 ± 0.46
2.91ProLeu: 2.91 ± 0.42
0.952ProMet: 0.952 ± 0.254
1.482ProAsn: 1.482 ± 0.314
1.64ProPro: 1.64 ± 0.425
1.693ProGln: 1.693 ± 0.302
2.328ProArg: 2.328 ± 0.327
2.91ProSer: 2.91 ± 0.333
2.646ProThr: 2.646 ± 0.342
3.757ProVal: 3.757 ± 0.66
1.111ProTrp: 1.111 ± 0.232
1.693ProTyr: 1.693 ± 0.297
0.0ProXaa: 0.0 ± 0.0
Gln
3.281GlnAla: 3.281 ± 0.503
0.423GlnCys: 0.423 ± 0.177
1.27GlnAsp: 1.27 ± 0.234
1.587GlnGlu: 1.587 ± 0.318
1.111GlnPhe: 1.111 ± 0.238
2.117GlnGly: 2.117 ± 0.319
0.317GlnHis: 0.317 ± 0.121
2.064GlnIle: 2.064 ± 0.365
1.27GlnLys: 1.27 ± 0.254
3.228GlnLeu: 3.228 ± 0.408
1.111GlnMet: 1.111 ± 0.259
1.534GlnAsn: 1.534 ± 0.3
1.111GlnPro: 1.111 ± 0.29
0.952GlnGln: 0.952 ± 0.252
1.746GlnArg: 1.746 ± 0.327
1.64GlnSer: 1.64 ± 0.312
1.323GlnThr: 1.323 ± 0.237
2.222GlnVal: 2.222 ± 0.487
0.423GlnTrp: 0.423 ± 0.127
1.111GlnTyr: 1.111 ± 0.271
0.0GlnXaa: 0.0 ± 0.0
Arg
3.334ArgAla: 3.334 ± 0.365
0.423ArgCys: 0.423 ± 0.154
4.074ArgAsp: 4.074 ± 0.449
3.598ArgGlu: 3.598 ± 0.443
2.91ArgPhe: 2.91 ± 0.39
3.916ArgGly: 3.916 ± 0.361
1.005ArgHis: 1.005 ± 0.221
3.651ArgIle: 3.651 ± 0.481
4.656ArgLys: 4.656 ± 0.456
5.133ArgLeu: 5.133 ± 0.465
1.534ArgMet: 1.534 ± 0.26
2.222ArgAsn: 2.222 ± 0.317
2.434ArgPro: 2.434 ± 0.421
1.958ArgGln: 1.958 ± 0.349
3.334ArgArg: 3.334 ± 0.526
2.963ArgSer: 2.963 ± 0.383
2.54ArgThr: 2.54 ± 0.387
4.233ArgVal: 4.233 ± 0.543
0.423ArgTrp: 0.423 ± 0.115
1.482ArgTyr: 1.482 ± 0.252
0.0ArgXaa: 0.0 ± 0.0
Ser
5.503SerAla: 5.503 ± 0.732
0.688SerCys: 0.688 ± 0.241
4.021SerAsp: 4.021 ± 0.486
4.339SerGlu: 4.339 ± 0.403
3.016SerPhe: 3.016 ± 0.437
5.45SerGly: 5.45 ± 0.494
1.164SerHis: 1.164 ± 0.296
3.545SerIle: 3.545 ± 0.42
3.492SerLys: 3.492 ± 0.411
5.45SerLeu: 5.45 ± 0.558
1.905SerMet: 1.905 ± 0.289
2.328SerAsn: 2.328 ± 0.439
2.751SerPro: 2.751 ± 0.498
1.852SerGln: 1.852 ± 0.436
3.175SerArg: 3.175 ± 0.337
4.498SerSer: 4.498 ± 0.573
2.487SerThr: 2.487 ± 0.377
4.815SerVal: 4.815 ± 0.628
0.847SerTrp: 0.847 ± 0.196
2.699SerTyr: 2.699 ± 0.431
0.0SerXaa: 0.0 ± 0.0
Thr
5.715ThrAla: 5.715 ± 0.581
0.37ThrCys: 0.37 ± 0.139
3.651ThrAsp: 3.651 ± 0.32
2.963ThrGlu: 2.963 ± 0.373
2.011ThrPhe: 2.011 ± 0.343
3.81ThrGly: 3.81 ± 0.45
1.429ThrHis: 1.429 ± 0.305
3.492ThrIle: 3.492 ± 0.398
2.487ThrLys: 2.487 ± 0.369
4.868ThrLeu: 4.868 ± 0.56
1.323ThrMet: 1.323 ± 0.3
1.958ThrAsn: 1.958 ± 0.24
3.439ThrPro: 3.439 ± 0.471
1.799ThrGln: 1.799 ± 0.312
2.963ThrArg: 2.963 ± 0.411
3.228ThrSer: 3.228 ± 0.495
3.228ThrThr: 3.228 ± 0.512
4.762ThrVal: 4.762 ± 0.535
1.058ThrTrp: 1.058 ± 0.193
1.693ThrTyr: 1.693 ± 0.51
0.0ThrXaa: 0.0 ± 0.0
Val
7.408ValAla: 7.408 ± 0.628
0.794ValCys: 0.794 ± 0.242
5.344ValAsp: 5.344 ± 0.466
5.291ValGlu: 5.291 ± 0.481
3.281ValPhe: 3.281 ± 0.425
4.551ValGly: 4.551 ± 0.593
1.058ValHis: 1.058 ± 0.265
4.921ValIle: 4.921 ± 0.545
5.503ValLys: 5.503 ± 0.643
3.916ValLeu: 3.916 ± 0.442
1.482ValMet: 1.482 ± 0.317
3.704ValAsn: 3.704 ± 0.393
3.545ValPro: 3.545 ± 0.526
2.117ValGln: 2.117 ± 0.242
4.392ValArg: 4.392 ± 0.567
4.974ValSer: 4.974 ± 0.608
5.344ValThr: 5.344 ± 0.508
5.715ValVal: 5.715 ± 0.586
1.111ValTrp: 1.111 ± 0.278
1.905ValTyr: 1.905 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
1.005TrpAla: 1.005 ± 0.189
0.212TrpCys: 0.212 ± 0.104
0.952TrpAsp: 0.952 ± 0.197
1.217TrpGlu: 1.217 ± 0.227
0.741TrpPhe: 0.741 ± 0.191
0.952TrpGly: 0.952 ± 0.242
0.106TrpHis: 0.106 ± 0.065
0.529TrpIle: 0.529 ± 0.163
0.582TrpLys: 0.582 ± 0.123
1.376TrpLeu: 1.376 ± 0.255
0.265TrpMet: 0.265 ± 0.129
0.635TrpAsn: 0.635 ± 0.195
0.582TrpPro: 0.582 ± 0.156
0.582TrpGln: 0.582 ± 0.157
0.9TrpArg: 0.9 ± 0.261
0.688TrpSer: 0.688 ± 0.194
0.741TrpThr: 0.741 ± 0.232
1.429TrpVal: 1.429 ± 0.384
0.265TrpTrp: 0.265 ± 0.125
0.529TrpTyr: 0.529 ± 0.148
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.54TyrAla: 2.54 ± 0.29
0.476TyrCys: 0.476 ± 0.13
2.434TyrAsp: 2.434 ± 0.286
2.275TyrGlu: 2.275 ± 0.351
1.64TyrPhe: 1.64 ± 0.331
2.54TyrGly: 2.54 ± 0.393
0.741TyrHis: 0.741 ± 0.224
1.376TyrIle: 1.376 ± 0.26
1.482TyrLys: 1.482 ± 0.295
2.169TyrLeu: 2.169 ± 0.396
0.794TyrMet: 0.794 ± 0.229
1.323TyrAsn: 1.323 ± 0.303
1.693TyrPro: 1.693 ± 0.346
1.005TyrGln: 1.005 ± 0.223
2.117TyrArg: 2.117 ± 0.306
2.646TyrSer: 2.646 ± 0.307
1.693TyrThr: 1.693 ± 0.355
2.804TyrVal: 2.804 ± 0.4
0.582TyrTrp: 0.582 ± 0.163
1.058TyrTyr: 1.058 ± 0.198
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (18900 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski