Vibrio phage vB_VmeM-Yong XC31
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 318 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B7EYL4|A0A6B7EYL4_9CAUD Uncharacterized protein OS=Vibrio phage vB_VmeM-Yong XC31 OX=2500766 PE=4 SV=1
MM1 pKa = 7.77 SIQITEE7 pKa = 4.46 PVNSTPVTDD16 pKa = 6.02 LMKK19 pKa = 10.37 GWCKK23 pKa = 10.18 GLEE26 pKa = 3.94 VHH28 pKa = 6.71 VPQSLPGFSLDD39 pKa = 3.38 VVPYY43 pKa = 10.52 SDD45 pKa = 4.42 YY46 pKa = 11.53 FEE48 pKa = 6.69 DD49 pKa = 6.31 DD50 pKa = 3.48 EE51 pKa = 7.25 DD52 pKa = 4.09 EE53 pKa = 4.47 DD54 pKa = 4.05
Molecular weight: 6.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.786
IPC2_protein 3.706
IPC_protein 3.605
Toseland 3.414
ProMoST 3.706
Dawson 3.617
Bjellqvist 3.872
Wikipedia 3.592
Rodwell 3.452
Grimsley 3.338
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.745
Patrickios 1.837
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|A0A6B7EWF3|A0A6B7EWF3_9CAUD Uncharacterized protein OS=Vibrio phage vB_VmeM-Yong XC31 OX=2500766 PE=4 SV=1
MM1 pKa = 6.8 KK2 pKa = 10.01 TKK4 pKa = 10.25 IVQADD9 pKa = 3.37 STGPMEE15 pKa = 5.7 EE16 pKa = 4.32 VFQPTAAYY24 pKa = 10.02 GPGDD28 pKa = 3.96 LVYY31 pKa = 10.83 KK32 pKa = 10.42 DD33 pKa = 3.29 GQYY36 pKa = 10.81 YY37 pKa = 9.34 RR38 pKa = 11.84 VVEE41 pKa = 4.42 PYY43 pKa = 9.73 LKK45 pKa = 10.5 KK46 pKa = 10.22 IAGEE50 pKa = 4.06 RR51 pKa = 11.84 KK52 pKa = 9.06 RR53 pKa = 11.84 DD54 pKa = 3.5 RR55 pKa = 11.84 LTKK58 pKa = 9.61 PKK60 pKa = 10.44 RR61 pKa = 11.84 RR62 pKa = 11.84 VAKK65 pKa = 10.24 IKK67 pKa = 10.5 ASRR70 pKa = 11.84 KK71 pKa = 9.14 AKK73 pKa = 9.59 RR74 pKa = 11.84 QSRR77 pKa = 11.84 RR78 pKa = 11.84 NNRR81 pKa = 11.84 RR82 pKa = 2.92
Molecular weight: 9.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 10.014
IPC_protein 10.774
Toseland 10.965
ProMoST 10.847
Dawson 11.038
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.374
Grimsley 11.082
Solomon 11.169
Lehninger 11.14
Nozaki 10.935
DTASelect 10.745
Thurlkill 10.95
EMBOSS 11.359
Sillero 10.965
Patrickios 11.096
IPC_peptide 11.184
IPC2_peptide 9.502
IPC2.peptide.svr19 8.423
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
318
0
318
89916
39
1901
282.8
31.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.795 ± 0.165
1.045 ± 0.051
6.347 ± 0.085
7.52 ± 0.144
4.36 ± 0.079
6.444 ± 0.148
2.238 ± 0.066
5.601 ± 0.086
5.944 ± 0.136
8.57 ± 0.12
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.686 ± 0.068
4.591 ± 0.084
3.904 ± 0.078
3.288 ± 0.067
5.198 ± 0.093
6.208 ± 0.096
6.132 ± 0.108
6.888 ± 0.106
1.458 ± 0.061
3.784 ± 0.075
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here