Ceratocystis fimbriata f. sp. platani

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Microascales; Ceratocystidaceae; Ceratocystis

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5620 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F8BTT5|A0A0F8BTT5_CERFI Indoleamine 2 3-dioxygenase OS=Ceratocystis fimbriata f. sp. platani OX=88771 GN=BNA2 PE=3 SV=1
MM1 pKa = 8.14DD2 pKa = 5.65GGTSTDD8 pKa = 3.3GGDD11 pKa = 3.26RR12 pKa = 11.84SNNFTFEE19 pKa = 4.07LMAGDD24 pKa = 5.14DD25 pKa = 3.82EE26 pKa = 6.9SIDD29 pKa = 3.59EE30 pKa = 4.7DD31 pKa = 3.98LNRR34 pKa = 11.84SYY36 pKa = 11.42GRR38 pKa = 11.84QHH40 pKa = 7.0RR41 pKa = 11.84GFEE44 pKa = 4.01NSFDD48 pKa = 3.82SSLSDD53 pKa = 4.65GSDD56 pKa = 3.45PDD58 pKa = 6.01DD59 pKa = 6.72DD60 pKa = 6.99DD61 pKa = 7.77DD62 pKa = 7.79DD63 pKa = 7.51DD64 pKa = 7.04DD65 pKa = 7.3DD66 pKa = 4.88EE67 pKa = 7.04DD68 pKa = 4.98NEE70 pKa = 4.73SSHH73 pKa = 6.84PWPHH77 pKa = 6.15HH78 pKa = 5.81QNDD81 pKa = 4.21FAPPYY86 pKa = 9.18YY87 pKa = 10.39GRR89 pKa = 11.84PPTPLPPSPSITSLLRR105 pKa = 11.84PSRR108 pKa = 11.84PTTPDD113 pKa = 2.95ASDD116 pKa = 4.92DD117 pKa = 3.66EE118 pKa = 4.92LEE120 pKa = 4.42PVPRR124 pKa = 11.84AAPRR128 pKa = 11.84VPTYY132 pKa = 10.26EE133 pKa = 3.77YY134 pKa = 11.02YY135 pKa = 11.26GFVLYY140 pKa = 10.61LFSSLCFLTYY150 pKa = 10.59LLWAFLPSPFLHH162 pKa = 6.81ALGITYY168 pKa = 10.55YY169 pKa = 10.05PDD171 pKa = 3.21RR172 pKa = 11.84WWALAMPTFLVVTLIYY188 pKa = 10.12IYY190 pKa = 10.8VALGSYY196 pKa = 7.87NTEE199 pKa = 3.77ILTLPLTAVEE209 pKa = 4.23TVIDD213 pKa = 4.21DD214 pKa = 4.15AAQVALVDD222 pKa = 3.76AAGSLRR228 pKa = 11.84DD229 pKa = 3.83GSRR232 pKa = 11.84MTPAKK237 pKa = 9.49IDD239 pKa = 3.42VMKK242 pKa = 10.85GYY244 pKa = 11.16GDD246 pKa = 4.65LATIWSEE253 pKa = 3.92GTDD256 pKa = 3.09AVMDD260 pKa = 3.74IPLGAVCEE268 pKa = 4.11VLYY271 pKa = 11.11ARR273 pKa = 11.84GPEE276 pKa = 4.08VFDD279 pKa = 4.34WEE281 pKa = 4.64DD282 pKa = 3.91DD283 pKa = 3.88YY284 pKa = 12.35NEE286 pKa = 3.96

Molecular weight: 31.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F8B468|A0A0F8B468_CERFI Uncharacterized protein OS=Ceratocystis fimbriata f. sp. platani OX=88771 GN=CFO_g1104 PE=4 SV=1
MM1 pKa = 6.86QRR3 pKa = 11.84PRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.45PKK9 pKa = 8.16QQRR12 pKa = 11.84KK13 pKa = 8.12PSSRR17 pKa = 11.84LNNSSSNSSSSSKK30 pKa = 9.65RR31 pKa = 11.84RR32 pKa = 11.84HH33 pKa = 4.69RR34 pKa = 11.84HH35 pKa = 5.29KK36 pKa = 10.7PMQRR40 pKa = 11.84PKK42 pKa = 10.36LQPRR46 pKa = 11.84HH47 pKa = 5.93KK48 pKa = 9.96LQPRR52 pKa = 11.84LKK54 pKa = 9.05PLHH57 pKa = 6.19RR58 pKa = 11.84LKK60 pKa = 10.56PLHH63 pKa = 6.74RR64 pKa = 11.84LKK66 pKa = 10.36QQRR69 pKa = 11.84KK70 pKa = 8.54LKK72 pKa = 8.2QQRR75 pKa = 11.84KK76 pKa = 8.37LSNRR80 pKa = 11.84LSNRR84 pKa = 11.84LSNRR88 pKa = 11.84PNNSNNSSNNNNNNNNNNNNNNNNNNSSNNSSNNSSNNSRR128 pKa = 11.84LSSNNNSSSSSNNNSSSSIKK148 pKa = 10.42YY149 pKa = 8.8NNHH152 pKa = 4.55NKK154 pKa = 9.31RR155 pKa = 11.84RR156 pKa = 11.84NRR158 pKa = 11.84SNSTNPNPISSSSSNNLSLPIIIPLSSPLLNHH190 pKa = 6.49TT191 pKa = 4.49

Molecular weight: 21.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5620

0

5620

2778377

66

4749

494.4

54.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.344 ± 0.037

1.053 ± 0.01

6.013 ± 0.024

6.392 ± 0.041

3.528 ± 0.021

6.521 ± 0.033

2.281 ± 0.015

4.919 ± 0.02

5.223 ± 0.034

8.523 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.494 ± 0.015

3.848 ± 0.017

5.703 ± 0.033

4.013 ± 0.032

5.782 ± 0.024

8.325 ± 0.038

6.016 ± 0.025

6.118 ± 0.021

1.266 ± 0.011

2.637 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski