Bipolaris zeicola 26-R-13

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris; Bipolaris zeicola

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6XSB4|W6XSB4_COCCA 18S rRNA factor 2 OS=Bipolaris zeicola 26-R-13 OX=930089 GN=COCCADRAFT_40951 PE=3 SV=1
MM1 pKa = 7.35SVTHH5 pKa = 7.13IIYY8 pKa = 9.9YY9 pKa = 9.77NCGHH13 pKa = 6.99LRR15 pKa = 11.84LIVSHH20 pKa = 6.48KK21 pKa = 10.2HH22 pKa = 6.95DD23 pKa = 4.42GDD25 pKa = 3.74EE26 pKa = 5.14CPTNMPDD33 pKa = 3.21TTTTPEE39 pKa = 3.61NCNRR43 pKa = 11.84CMSPKK48 pKa = 10.26SGSDD52 pKa = 3.5SEE54 pKa = 4.68GASPDD59 pKa = 3.3MYY61 pKa = 10.88FEE63 pKa = 5.37SDD65 pKa = 3.42FSSISVSSGDD75 pKa = 4.18ATDD78 pKa = 3.43SDD80 pKa = 4.87YY81 pKa = 11.9NGDD84 pKa = 4.89DD85 pKa = 3.99EE86 pKa = 7.46DD87 pKa = 6.64DD88 pKa = 5.07DD89 pKa = 6.32GSDD92 pKa = 5.39DD93 pKa = 3.79EE94 pKa = 4.85GTDD97 pKa = 3.51EE98 pKa = 5.24GAHH101 pKa = 5.75QSDD104 pKa = 4.18EE105 pKa = 4.3EE106 pKa = 4.18MLSDD110 pKa = 4.63NSNDD114 pKa = 3.34VPALRR119 pKa = 11.84LRR121 pKa = 11.84NVNPTEE127 pKa = 3.92NCTNPWSDD135 pKa = 3.05MSEE138 pKa = 3.95LL139 pKa = 3.76

Molecular weight: 15.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6XNT0|W6XNT0_COCCA F-box domain-containing protein OS=Bipolaris zeicola 26-R-13 OX=930089 GN=COCCADRAFT_107717 PE=3 SV=1
MM1 pKa = 7.53PLARR5 pKa = 11.84RR6 pKa = 11.84SAPRR10 pKa = 11.84TTRR13 pKa = 11.84TTARR17 pKa = 11.84PSLKK21 pKa = 8.96TRR23 pKa = 11.84LMGGRR28 pKa = 11.84KK29 pKa = 7.34TRR31 pKa = 11.84THH33 pKa = 6.09TKK35 pKa = 9.63RR36 pKa = 11.84APAGTTTTTTTTRR49 pKa = 11.84TTRR52 pKa = 11.84TTGGHH57 pKa = 6.59HH58 pKa = 5.97GHH60 pKa = 7.37AAPVHH65 pKa = 4.63HH66 pKa = 7.34HH67 pKa = 6.34KK68 pKa = 10.63RR69 pKa = 11.84HH70 pKa = 4.81ATMGDD75 pKa = 3.45KK76 pKa = 10.91VSGMMMKK83 pKa = 10.48LRR85 pKa = 11.84GSLTRR90 pKa = 11.84RR91 pKa = 11.84PGLKK95 pKa = 10.1AAGTRR100 pKa = 11.84RR101 pKa = 11.84MHH103 pKa = 5.92GTDD106 pKa = 2.72GRR108 pKa = 11.84NARR111 pKa = 11.84RR112 pKa = 11.84VYY114 pKa = 10.89

Molecular weight: 12.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12851

0

12851

5498859

49

8312

427.9

47.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.731 ± 0.021

1.302 ± 0.01

5.566 ± 0.015

6.08 ± 0.024

3.671 ± 0.013

6.73 ± 0.021

2.477 ± 0.011

4.897 ± 0.017

5.082 ± 0.021

8.638 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.009

3.759 ± 0.012

6.16 ± 0.025

4.144 ± 0.015

6.0 ± 0.019

8.108 ± 0.027

6.08 ± 0.014

6.035 ± 0.016

1.473 ± 0.008

2.829 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski