Tomato yellow leaf curl Indonesia virus-[Lembang]
Average proteome isoelectric point is 8.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9QD51|Q9QD51_9GEMI Protein V2 OS=Tomato yellow leaf curl Indonesia virus-[Lembang] OX=395349 GN=V2 PE=3 SV=1
MM1 pKa = 8.06 WDD3 pKa = 3.35 PLLNEE8 pKa = 4.99 FPDD11 pKa = 3.86 SVHH14 pKa = 6.81 GFRR17 pKa = 11.84 CMLAIKK23 pKa = 9.6 YY24 pKa = 8.46 LQGVEE29 pKa = 3.81 ATYY32 pKa = 11.13 APDD35 pKa = 3.34 TVGYY39 pKa = 10.59 DD40 pKa = 3.39 LVRR43 pKa = 11.84 DD44 pKa = 4.64 LISVVRR50 pKa = 11.84 ASNYY54 pKa = 10.28 AEE56 pKa = 3.93 ACRR59 pKa = 11.84 RR60 pKa = 11.84 YY61 pKa = 10.06 SLFRR65 pKa = 11.84 SRR67 pKa = 11.84 IEE69 pKa = 4.04 STPSSQLRR77 pKa = 11.84 QPRR80 pKa = 11.84 YY81 pKa = 9.33 QPCCCTHH88 pKa = 6.58 CPRR91 pKa = 11.84 HH92 pKa = 5.91 KK93 pKa = 10.64 SKK95 pKa = 10.98 EE96 pKa = 4.01 VLDD99 pKa = 4.98 FSAYY103 pKa = 10.04 VPEE106 pKa = 4.69 AQDD109 pKa = 4.72 LPDD112 pKa = 3.76 VPNSS116 pKa = 3.4
Molecular weight: 13.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.681
IPC2_protein 5.931
IPC_protein 5.982
Toseland 6.135
ProMoST 6.287
Dawson 6.313
Bjellqvist 6.262
Wikipedia 6.326
Rodwell 6.3
Grimsley 6.198
Solomon 6.313
Lehninger 6.313
Nozaki 6.561
DTASelect 6.737
Thurlkill 6.751
EMBOSS 6.737
Sillero 6.693
Patrickios 3.058
IPC_peptide 6.326
IPC2_peptide 6.664
IPC2.peptide.svr19 6.581
Protein with the highest isoelectric point:
>tr|Q9QD51|Q9QD51_9GEMI Protein V2 OS=Tomato yellow leaf curl Indonesia virus-[Lembang] OX=395349 GN=V2 PE=3 SV=1
MM1 pKa = 7.76 LKK3 pKa = 10.25 RR4 pKa = 11.84 AGDD7 pKa = 3.5 IAFSGPVSKK16 pKa = 10.59 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNYY23 pKa = 9.91 DD24 pKa = 3.02 SPGTSRR30 pKa = 11.84 AAVLTALGINRR41 pKa = 11.84 KK42 pKa = 9.5 KK43 pKa = 10.54 SWTSRR48 pKa = 11.84 PMYY51 pKa = 9.55 RR52 pKa = 11.84 KK53 pKa = 8.49 PRR55 pKa = 11.84 IYY57 pKa = 10.79 RR58 pKa = 11.84 MYY60 pKa = 9.46 RR61 pKa = 11.84 TADD64 pKa = 3.31 VPRR67 pKa = 11.84 GCEE70 pKa = 4.41 GPCKK74 pKa = 9.83 IQSFEE79 pKa = 4.02 SRR81 pKa = 11.84 HH82 pKa = 6.29 DD83 pKa = 3.27 IAHH86 pKa = 6.41 TGKK89 pKa = 10.37 VMCVTDD95 pKa = 3.4 VTRR98 pKa = 11.84 GGGLTHH104 pKa = 6.43 RR105 pKa = 11.84 TGKK108 pKa = 10.01 RR109 pKa = 11.84 FCVKK113 pKa = 10.06 SLYY116 pKa = 10.17 ILGKK120 pKa = 9.29 IWMDD124 pKa = 3.37 EE125 pKa = 4.04 NIKK128 pKa = 9.32 TKK130 pKa = 10.61 NHH132 pKa = 5.92 TNTVMFYY139 pKa = 9.52 VVRR142 pKa = 11.84 DD143 pKa = 3.66 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 9.21 GTPQDD152 pKa = 3.82 FGQVFNMFDD161 pKa = 3.99 NEE163 pKa = 4.11 PSTATVKK170 pKa = 10.85 NDD172 pKa = 2.82 LRR174 pKa = 11.84 DD175 pKa = 3.42 RR176 pKa = 11.84 FQVLRR181 pKa = 11.84 KK182 pKa = 8.36 FTATVVGGQYY192 pKa = 10.67 ACKK195 pKa = 9.94 EE196 pKa = 3.83 QTLVRR201 pKa = 11.84 KK202 pKa = 9.44 FMRR205 pKa = 11.84 LNNYY209 pKa = 7.92 VVYY212 pKa = 10.63 NHH214 pKa = 6.28 QEE216 pKa = 3.67 TAKK219 pKa = 10.86 YY220 pKa = 9.93 EE221 pKa = 4.15 NHH223 pKa = 6.41 TEE225 pKa = 3.99 NALLLYY231 pKa = 8.57 MASTHH236 pKa = 6.41 ASNPVYY242 pKa = 9.86 ATLKK246 pKa = 9.47 IRR248 pKa = 11.84 IYY250 pKa = 10.59 FYY252 pKa = 11.38 DD253 pKa = 4.2 SITNN257 pKa = 3.6
Molecular weight: 29.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.575
IPC_protein 9.853
Toseland 10.087
ProMoST 9.853
Dawson 10.321
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.643
Grimsley 10.409
Solomon 10.35
Lehninger 10.306
Nozaki 10.116
DTASelect 10.028
Thurlkill 10.16
EMBOSS 10.511
Sillero 10.233
Patrickios 10.058
IPC_peptide 10.35
IPC2_peptide 8.931
IPC2.peptide.svr19 8.515
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1069
65
362
178.2
20.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.332 ± 0.593
2.245 ± 0.468
5.051 ± 0.448
4.022 ± 0.675
4.397 ± 0.524
4.864 ± 0.499
3.742 ± 0.449
5.426 ± 0.975
6.08 ± 0.749
7.858 ± 0.729
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.152 ± 0.503
6.268 ± 0.638
5.987 ± 0.733
4.303 ± 0.652
7.016 ± 1.198
7.858 ± 1.403
5.893 ± 0.845
5.893 ± 1.126
1.31 ± 0.179
4.303 ± 0.623
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here