Mycobacterium phage Lokk

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286MQA1|A0A286MQA1_9CAUD Uncharacterized protein OS=Mycobacterium phage Lokk OX=2015821 GN=82 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.4AIILACAFAAGGLIGQHH19 pKa = 5.95IPVTVNSTEE28 pKa = 3.54VRR30 pKa = 11.84TVDD33 pKa = 4.14GIDD36 pKa = 3.6YY37 pKa = 7.63PVCAVEE43 pKa = 5.17DD44 pKa = 4.64CSDD47 pKa = 3.31QPGQIGIWIDD57 pKa = 3.69PDD59 pKa = 3.84TGNQWLSHH67 pKa = 6.31GEE69 pKa = 3.87TSQLIEE75 pKa = 3.99RR76 pKa = 4.68

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286MQA9|A0A286MQA9_9CAUD Uncharacterized protein OS=Mycobacterium phage Lokk OX=2015821 GN=90 PE=4 SV=1
MM1 pKa = 6.8TATRR5 pKa = 11.84KK6 pKa = 9.44EE7 pKa = 3.92CMMNKK12 pKa = 7.24QQRR15 pKa = 11.84VYY17 pKa = 10.32IDD19 pKa = 2.92GRR21 pKa = 11.84VYY23 pKa = 9.62VARR26 pKa = 11.84GNGYY30 pKa = 9.5VRR32 pKa = 11.84VRR34 pKa = 3.45

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

15849

22

842

178.1

19.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.83 ± 0.433

0.928 ± 0.118

6.436 ± 0.23

6.631 ± 0.33

3.407 ± 0.184

8.329 ± 0.446

1.905 ± 0.166

5.155 ± 0.187

4.669 ± 0.238

8.127 ± 0.378

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.125

3.256 ± 0.192

5.571 ± 0.258

3.868 ± 0.232

6.24 ± 0.407

5.489 ± 0.216

6.101 ± 0.226

7.105 ± 0.251

1.861 ± 0.151

2.789 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski