Hydrogenivirga caldilitoris

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Aquificaceae; Hydrogenivirga

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1885 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A497XMM9|A0A497XMM9_9AQUI Uncharacterized protein OS=Hydrogenivirga caldilitoris OX=246264 GN=BCF55_0409 PE=4 SV=1
MM1 pKa = 7.48SKK3 pKa = 10.59KK4 pKa = 10.08FLLSAVFSAGVLSAGALAGDD24 pKa = 3.4MQVLNEE30 pKa = 4.47EE31 pKa = 4.16EE32 pKa = 4.74LEE34 pKa = 4.23DD35 pKa = 4.33VSAQGLQVIEE45 pKa = 3.8NDD47 pKa = 2.75NRR49 pKa = 11.84QFAEE53 pKa = 4.48INGQNNNLDD62 pKa = 3.89SVQLNDD68 pKa = 3.39NAQLEE73 pKa = 4.36AQAGGIINVAKK84 pKa = 9.32TALNAAVNLLADD96 pKa = 4.38GPEE99 pKa = 4.65PGPKK103 pKa = 9.65PPPQNGDD110 pKa = 2.94TKK112 pKa = 10.72YY113 pKa = 9.82FNYY116 pKa = 10.3YY117 pKa = 10.27FSQTNVQVAMNHH129 pKa = 5.24VNTANNADD137 pKa = 3.92AEE139 pKa = 4.46EE140 pKa = 4.16EE141 pKa = 3.97LAIAGNLNKK150 pKa = 8.79EE151 pKa = 4.44TQSIDD156 pKa = 3.1NNGNIRR162 pKa = 11.84EE163 pKa = 3.93QDD165 pKa = 3.38NNNNSVQLNDD175 pKa = 3.59SAQSGASGLMIEE187 pKa = 4.69NAAISAVNTGMNIFVAGDD205 pKa = 3.29VDD207 pKa = 4.08STEE210 pKa = 4.04GHH212 pKa = 4.42QTNTQVAKK220 pKa = 11.21NMGNYY225 pKa = 10.22AEE227 pKa = 4.86AQAGDD232 pKa = 3.66QPIAVAGNAEE242 pKa = 4.39LGVTQNINNQYY253 pKa = 9.9QSEE256 pKa = 4.47GEE258 pKa = 4.04PNNIYY263 pKa = 10.88DD264 pKa = 4.25QDD266 pKa = 3.9NNNNSVQLNDD276 pKa = 3.59SAQSGASAVSMLNVANSAYY295 pKa = 10.59NSGLNVMAMANLSNSLMTQNNSQTAEE321 pKa = 3.84SHH323 pKa = 5.94VNVASAEE330 pKa = 4.03ADD332 pKa = 3.29NGVALAGNLNKK343 pKa = 8.77QTQNVHH349 pKa = 5.81NAVADD354 pKa = 3.98DD355 pKa = 4.04FVGLGVIEE363 pKa = 4.32EE364 pKa = 4.07QDD366 pKa = 3.43NNNNSVQLNDD376 pKa = 3.59SAQSGASALDD386 pKa = 3.58MTNSAVSAGNTGMNVMAATTVSTSEE411 pKa = 3.72IHH413 pKa = 5.7QGNYY417 pKa = 7.66QSARR421 pKa = 11.84NFDD424 pKa = 3.72NEE426 pKa = 3.32ARR428 pKa = 11.84TYY430 pKa = 10.73NGNPEE435 pKa = 4.13SEE437 pKa = 5.19DD438 pKa = 3.03IAVATNAEE446 pKa = 4.24IAGEE450 pKa = 4.0PGQYY454 pKa = 9.75VHH456 pKa = 6.66TVHH459 pKa = 6.65ATIGDD464 pKa = 3.68QNNNNNSVQLNDD476 pKa = 4.37DD477 pKa = 3.98AQSSASALFMTNMAQSAVNSGLNLLFVDD505 pKa = 4.95GNVDD509 pKa = 3.81DD510 pKa = 4.54STVSQTNEE518 pKa = 3.54QFASNHH524 pKa = 5.12NNYY527 pKa = 8.3ATANALAVAINLNKK541 pKa = 9.49QRR543 pKa = 11.84QIIEE547 pKa = 3.95NCFCSDD553 pKa = 5.23LSQTDD558 pKa = 2.95QDD560 pKa = 3.83NNMNSVQLNDD570 pKa = 3.33NAQSYY575 pKa = 10.21ASGWMIVNAATSAVNSGTNLAVVNGVVSGSSVTQTNVQTSVNFSNQASGNTAIAGNAEE633 pKa = 4.14IGSLFIPIGFF643 pKa = 3.97

Molecular weight: 67.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A497XMI3|A0A497XMI3_9AQUI Prepilin-type N-terminal cleavage/methylation domain-containing protein OS=Hydrogenivirga caldilitoris OX=246264 GN=BCF55_0390 PE=4 SV=1
MM1 pKa = 7.76PEE3 pKa = 3.81ALKK6 pKa = 10.3PHH8 pKa = 6.36ISNRR12 pKa = 11.84KK13 pKa = 8.03KK14 pKa = 10.01KK15 pKa = 10.01RR16 pKa = 11.84KK17 pKa = 9.55SGFLARR23 pKa = 11.84MKK25 pKa = 10.55SRR27 pKa = 11.84AGRR30 pKa = 11.84KK31 pKa = 8.0VIKK34 pKa = 10.02RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84QKK39 pKa = 10.21GRR41 pKa = 11.84KK42 pKa = 8.77RR43 pKa = 11.84LAPP46 pKa = 3.99

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1885

0

1885

561480

30

1582

297.9

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.222 ± 0.052

0.837 ± 0.023

4.67 ± 0.041

9.048 ± 0.082

4.915 ± 0.054

7.314 ± 0.048

1.575 ± 0.021

6.823 ± 0.049

7.407 ± 0.066

10.973 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.022

3.294 ± 0.033

4.098 ± 0.037

2.114 ± 0.025

5.879 ± 0.05

5.443 ± 0.046

4.394 ± 0.034

8.15 ± 0.04

0.958 ± 0.021

3.796 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski