Rhodococcus phage Sleepyhead

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sleepyheadvirus; Rhodococcus virus Sleepyhead

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A515MHA4|A0A515MHA4_9CAUD Cro protein OS=Rhodococcus phage Sleepyhead OX=2591131 GN=41 PE=4 SV=1
MM1 pKa = 7.47AALGASLLLGGCGNDD16 pKa = 3.29ADD18 pKa = 4.76SVPSVQSSDD27 pKa = 3.32ASATASTSTPPALPTIGSTVANGDD51 pKa = 3.16ASITVVSVTSSDD63 pKa = 3.85AISFEE68 pKa = 4.17TDD70 pKa = 3.03SLNGQYY76 pKa = 7.77EE77 pKa = 4.31QKK79 pKa = 10.57AAPAGGKK86 pKa = 9.39FVVVEE91 pKa = 4.15TSVEE95 pKa = 4.32NIGKK99 pKa = 8.39TSMDD103 pKa = 3.64LTCNYY108 pKa = 9.32PIVARR113 pKa = 11.84VIDD116 pKa = 3.8SEE118 pKa = 4.05EE119 pKa = 4.06RR120 pKa = 11.84QFDD123 pKa = 4.0RR124 pKa = 11.84IDD126 pKa = 3.33YY127 pKa = 9.8IFRR130 pKa = 11.84VEE132 pKa = 4.91GNPGCNKK139 pKa = 9.76NLQPGFSAPMKK150 pKa = 9.35WVYY153 pKa = 9.94EE154 pKa = 4.32LPASATPEE162 pKa = 3.88LFGFITPGTQTIEE175 pKa = 3.88DD176 pKa = 3.79AAFVSLVEE184 pKa = 4.27DD185 pKa = 3.76QPP187 pKa = 4.01

Molecular weight: 19.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A515MHL0|A0A515MHL0_9CAUD Peptidase OS=Rhodococcus phage Sleepyhead OX=2591131 GN=39 PE=4 SV=1
MM1 pKa = 7.54GGLPTTARR9 pKa = 11.84IARR12 pKa = 11.84LFAFVVVIAGLVCGLAALPLFRR34 pKa = 11.84ADD36 pKa = 4.04AKK38 pKa = 10.54IDD40 pKa = 4.25GIAAPGIVIATKK52 pKa = 10.61VFADD56 pKa = 5.1PYY58 pKa = 8.03PLTWIVTVFGSNARR72 pKa = 11.84LIRR75 pKa = 4.18

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13807

38

1587

206.1

22.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.328 ± 0.751

0.753 ± 0.13

6.765 ± 0.241

5.932 ± 0.335

2.803 ± 0.121

8.394 ± 0.568

1.876 ± 0.208

5.121 ± 0.189

4.063 ± 0.231

7.713 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.079 ± 0.119

3.187 ± 0.195

5.323 ± 0.334

3.636 ± 0.183

6.917 ± 0.458

6.395 ± 0.258

6.873 ± 0.385

6.967 ± 0.234

1.709 ± 0.196

2.166 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski