Capybara microvirus Cap1_SP_60
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4N9|A0A4P8W4N9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_60 OX=2584785 PE=4 SV=1
MM1 pKa = 7.53 ATRR4 pKa = 11.84 KK5 pKa = 9.31 FQFEE9 pKa = 4.39 TMTAAGKK16 pKa = 10.58 KK17 pKa = 9.91 SVFDD21 pKa = 3.31 MSFNHH26 pKa = 7.18 IGTQDD31 pKa = 3.41 FGYY34 pKa = 10.45 LKK36 pKa = 10.16 PIGMNFLVPGDD47 pKa = 3.45 EE48 pKa = 3.88 WKK50 pKa = 10.81 INITQFTRR58 pKa = 11.84 LMPMPCPTFGKK69 pKa = 9.53 VDD71 pKa = 3.19 TRR73 pKa = 11.84 IRR75 pKa = 11.84 AFFVPFQTILNGWNDD90 pKa = 4.48 FIANRR95 pKa = 11.84 VSPSTGGEE103 pKa = 3.44 IAIPHH108 pKa = 5.23 VTNEE112 pKa = 3.51 WFTNWFVKK120 pKa = 10.43 KK121 pKa = 10.29 NAFILEE127 pKa = 4.35 DD128 pKa = 3.52 QDD130 pKa = 4.27 LPLYY134 pKa = 10.64 QEE136 pKa = 4.92 GSWDD140 pKa = 3.65 YY141 pKa = 10.53 YY142 pKa = 11.23 DD143 pKa = 4.56 IIVRR147 pKa = 11.84 EE148 pKa = 4.09 NDD150 pKa = 4.09 GLWHH154 pKa = 6.49 GLNLTPLGKK163 pKa = 9.79 RR164 pKa = 11.84 FYY166 pKa = 11.63 DD167 pKa = 3.38 MLLGCGINIQMCAYY181 pKa = 9.97 KK182 pKa = 9.65 STTEE186 pKa = 3.66 EE187 pKa = 4.68 AYY189 pKa = 9.81 TEE191 pKa = 3.68 IAQRR195 pKa = 11.84 KK196 pKa = 8.79 FSVLPIAGLYY206 pKa = 8.97 RR207 pKa = 11.84 WYY209 pKa = 9.61 TDD211 pKa = 3.2 WIVPSRR217 pKa = 11.84 FVDD220 pKa = 3.62 EE221 pKa = 4.18 YY222 pKa = 10.65 MPIRR226 pKa = 11.84 MLFVANEE233 pKa = 3.87 WSGEE237 pKa = 4.06 EE238 pKa = 4.97 LFTAMDD244 pKa = 3.33 QIYY247 pKa = 10.09 NVNPAALVLSTFYY260 pKa = 11.23 ADD262 pKa = 6.02 DD263 pKa = 3.74 MFSCAFKK270 pKa = 11.27 NPFGLEE276 pKa = 3.92 NQSMYY281 pKa = 10.84 DD282 pKa = 3.68 YY283 pKa = 10.82 NIQNPAIYY291 pKa = 9.87 SSEE294 pKa = 3.94 GTHH297 pKa = 5.6 MLGAEE302 pKa = 5.33 AITSIDD308 pKa = 3.59 PTLQAAKK315 pKa = 9.89 QGARR319 pKa = 11.84 IKK321 pKa = 10.48 RR322 pKa = 11.84 GEE324 pKa = 4.25 GEE326 pKa = 4.12 PSNGSTWGINHH337 pKa = 5.91 FTIKK341 pKa = 10.52 SLGVLQDD348 pKa = 3.4 LLNRR352 pKa = 11.84 GKK354 pKa = 10.21 IAGTKK359 pKa = 8.34 IQDD362 pKa = 4.23 YY363 pKa = 10.41 LWEE366 pKa = 4.43 TYY368 pKa = 10.21 GIKK371 pKa = 10.68 ANSDD375 pKa = 3.65 EE376 pKa = 5.04 LNLSLYY382 pKa = 10.38 LGSSQDD388 pKa = 3.19 IIKK391 pKa = 10.44 IGDD394 pKa = 3.48 VMATAGTEE402 pKa = 4.2 VNEE405 pKa = 4.34 LGQFAGKK412 pKa = 10.5 GIGSMRR418 pKa = 11.84 FGCTCKK424 pKa = 10.44 SGNKK428 pKa = 9.55 HH429 pKa = 5.51 GLLYY433 pKa = 10.69 LCSEE437 pKa = 4.52 INVDD441 pKa = 3.1 PSYY444 pKa = 11.26 YY445 pKa = 9.94 QGEE448 pKa = 4.37 KK449 pKa = 10.48 PEE451 pKa = 3.93 MRR453 pKa = 11.84 QLEE456 pKa = 4.26 RR457 pKa = 11.84 DD458 pKa = 3.49 DD459 pKa = 5.31 FYY461 pKa = 11.62 QPEE464 pKa = 4.16 FDD466 pKa = 3.77 NLGVEE471 pKa = 5.38 AISKK475 pKa = 9.74 AQIKK479 pKa = 10.59 AEE481 pKa = 3.79 WDD483 pKa = 3.55 NNGMNMYY490 pKa = 9.58 GYY492 pKa = 9.64 PIDD495 pKa = 4.33 SPDD498 pKa = 3.86 SIFGFTPRR506 pKa = 11.84 YY507 pKa = 9.58 SKK509 pKa = 11.46 YY510 pKa = 10.67 KK511 pKa = 9.97 FNNDD515 pKa = 3.11 IISGDD520 pKa = 3.39 FRR522 pKa = 11.84 LQSSRR527 pKa = 11.84 TLRR530 pKa = 11.84 SWYY533 pKa = 10.12 LSRR536 pKa = 11.84 NLEE539 pKa = 4.22 YY540 pKa = 9.66 ATKK543 pKa = 9.34 GTWTNINSNFCRR555 pKa = 11.84 QLVDD559 pKa = 4.06 IEE561 pKa = 4.41 INNYY565 pKa = 10.96 DD566 pKa = 3.8 YY567 pKa = 11.15 MFNAANNNSDD577 pKa = 2.56 HH578 pKa = 6.82 FYY580 pKa = 10.99 NVFSVGIKK588 pKa = 10.11 ALRR591 pKa = 11.84 PMEE594 pKa = 4.67 TISKK598 pKa = 10.27 ALEE601 pKa = 4.25 TEE603 pKa = 4.13 DD604 pKa = 3.74 TNDD607 pKa = 2.91 MGRR610 pKa = 11.84 KK611 pKa = 9.22 VRR613 pKa = 11.84 VSMNN617 pKa = 3.02
Molecular weight: 70.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.955
IPC2_protein 5.067
IPC_protein 5.016
Toseland 4.914
ProMoST 5.143
Dawson 4.991
Bjellqvist 5.13
Wikipedia 4.863
Rodwell 4.902
Grimsley 4.838
Solomon 4.991
Lehninger 4.952
Nozaki 5.105
DTASelect 5.283
Thurlkill 4.927
EMBOSS 4.902
Sillero 5.181
Patrickios 3.745
IPC_peptide 5.003
IPC2_peptide 5.169
IPC2.peptide.svr19 5.186
Protein with the highest isoelectric point:
>tr|A0A4P8W7I5|A0A4P8W7I5_9VIRU Helix-turn-helix XRE family-like protein OS=Capybara microvirus Cap1_SP_60 OX=2584785 PE=4 SV=1
MM1 pKa = 7.2 CTNPKK6 pKa = 8.91 TIKK9 pKa = 10.22 NPKK12 pKa = 8.36 IDD14 pKa = 3.74 YY15 pKa = 9.97 IKK17 pKa = 11.01 YY18 pKa = 8.51 YY19 pKa = 10.1 DD20 pKa = 3.57 KK21 pKa = 10.93 PYY23 pKa = 10.49 IQVPCGKK30 pKa = 10.08 CKK32 pKa = 10.36 EE33 pKa = 4.2 CQEE36 pKa = 4.34 LKK38 pKa = 10.28 NNEE41 pKa = 3.72 WLVRR45 pKa = 11.84 NFFEE49 pKa = 4.37 CKK51 pKa = 9.19 EE52 pKa = 4.22 AIRR55 pKa = 11.84 CGGYY59 pKa = 7.09 TQAYY63 pKa = 4.74 TTTYY67 pKa = 9.45 RR68 pKa = 11.84 DD69 pKa = 2.69 EE70 pKa = 6.1 DD71 pKa = 3.57 IPHH74 pKa = 6.51 NSFGVKK80 pKa = 9.92 CFSKK84 pKa = 10.83 EE85 pKa = 3.88 DD86 pKa = 3.23 VRR88 pKa = 11.84 KK89 pKa = 10.46 YY90 pKa = 10.49 IDD92 pKa = 4.54 AIKK95 pKa = 10.61 SDD97 pKa = 3.39 LRR99 pKa = 11.84 RR100 pKa = 11.84 TFGAEE105 pKa = 3.84 KK106 pKa = 10.38 INGKK110 pKa = 9.54 FKK112 pKa = 10.7 YY113 pKa = 10.37 YY114 pKa = 10.14 MSFEE118 pKa = 4.31 YY119 pKa = 10.61 GSKK122 pKa = 10.25 DD123 pKa = 2.63 WYY125 pKa = 10.79 FNKK128 pKa = 8.77 KK129 pKa = 6.43 TKK131 pKa = 10.09 KK132 pKa = 8.95 YY133 pKa = 8.91 QQATEE138 pKa = 4.23 RR139 pKa = 11.84 PHH141 pKa = 6.7 CHH143 pKa = 6.91 FIFHH147 pKa = 6.38 IQWKK151 pKa = 7.66 CTLGEE156 pKa = 3.77 RR157 pKa = 11.84 CVIKK161 pKa = 10.71 RR162 pKa = 11.84 ILKK165 pKa = 8.29 KK166 pKa = 9.85 HH167 pKa = 4.74 WVKK170 pKa = 11.29 GDD172 pKa = 3.08 ITYY175 pKa = 7.62 TTNTNDD181 pKa = 4.07 GIIDD185 pKa = 3.49 EE186 pKa = 4.33 RR187 pKa = 11.84 AIAGGIGAVRR197 pKa = 11.84 YY198 pKa = 7.18 LTNYY202 pKa = 10.13 LNEE205 pKa = 4.43 EE206 pKa = 4.14 KK207 pKa = 10.97 NPIPKK212 pKa = 9.45 EE213 pKa = 4.07 VKK215 pKa = 9.71 EE216 pKa = 4.16 GCQRR220 pKa = 11.84 IEE222 pKa = 3.93 KK223 pKa = 9.95 KK224 pKa = 10.31 IKK226 pKa = 9.2 FEE228 pKa = 3.62 QRR230 pKa = 11.84 YY231 pKa = 8.45 IYY233 pKa = 10.89 LMTAEE238 pKa = 4.95 KK239 pKa = 9.31 PQCEE243 pKa = 4.45 SKK245 pKa = 10.46 QEE247 pKa = 3.76 KK248 pKa = 10.34 AILYY252 pKa = 9.14 KK253 pKa = 10.31 VRR255 pKa = 11.84 EE256 pKa = 4.16 YY257 pKa = 11.68 NNVVKK262 pKa = 10.37 EE263 pKa = 4.12 KK264 pKa = 10.39 KK265 pKa = 9.74 IKK267 pKa = 9.05 NTEE270 pKa = 3.69 EE271 pKa = 3.89 RR272 pKa = 11.84 IKK274 pKa = 10.43 ILKK277 pKa = 9.63 RR278 pKa = 11.84 EE279 pKa = 3.87 KK280 pKa = 10.53 AKK282 pKa = 10.65 CIPCTMQSSGLGKK295 pKa = 10.13 SALLQDD301 pKa = 3.69 QNGKK305 pKa = 10.24 YY306 pKa = 9.94 IYY308 pKa = 9.09 NTYY311 pKa = 10.34 FNYY314 pKa = 10.9 AMAEE318 pKa = 3.96 KK319 pKa = 10.34 GEE321 pKa = 4.19 CFIDD325 pKa = 4.68 DD326 pKa = 4.31 FEE328 pKa = 4.51 LTRR331 pKa = 11.84 KK332 pKa = 9.15 KK333 pKa = 10.51 RR334 pKa = 11.84 KK335 pKa = 9.13 LPLYY339 pKa = 10.02 IEE341 pKa = 4.59 RR342 pKa = 11.84 KK343 pKa = 9.35 LFYY346 pKa = 10.79 KK347 pKa = 10.01 EE348 pKa = 4.38 VPCKK352 pKa = 9.89 KK353 pKa = 9.83 AKK355 pKa = 10.66 NGMTYY360 pKa = 11.1 KK361 pKa = 10.64 LNEE364 pKa = 4.19 AGKK367 pKa = 9.09 EE368 pKa = 3.95 MKK370 pKa = 10.07 RR371 pKa = 11.84 KK372 pKa = 9.54 RR373 pKa = 11.84 FHH375 pKa = 6.77 FFALALQKK383 pKa = 10.69 KK384 pKa = 7.97 IWNVGHH390 pKa = 6.48 YY391 pKa = 9.71 DD392 pKa = 3.82 YY393 pKa = 11.08 NTMYY397 pKa = 10.28 QYY399 pKa = 9.29 KK400 pKa = 9.28 TNEE403 pKa = 3.59 EE404 pKa = 3.7 KK405 pKa = 10.88 AQYY408 pKa = 11.19 KK409 pKa = 10.45 KK410 pKa = 9.54 MAKK413 pKa = 10.17 DD414 pKa = 2.98 ILANLKK420 pKa = 10.4 DD421 pKa = 3.59 FGMIEE426 pKa = 4.07 RR427 pKa = 11.84 YY428 pKa = 7.41 VTYY431 pKa = 10.51 KK432 pKa = 10.76 GVEE435 pKa = 4.12 KK436 pKa = 10.64 KK437 pKa = 9.94 DD438 pKa = 3.12 TDD440 pKa = 3.66 IINNYY445 pKa = 7.3 GTLEE449 pKa = 4.19 RR450 pKa = 11.84 EE451 pKa = 4.8 CQDD454 pKa = 2.52 TWNILYY460 pKa = 9.95 KK461 pKa = 9.89 YY462 pKa = 9.59 EE463 pKa = 4.13 KK464 pKa = 10.77 GKK466 pKa = 10.77 EE467 pKa = 3.99 EE468 pKa = 3.98 EE469 pKa = 4.28 NAEE472 pKa = 3.74 QGYY475 pKa = 9.35 KK476 pKa = 10.35 FKK478 pKa = 11.23 EE479 pKa = 3.99 FLEE482 pKa = 5.02 DD483 pKa = 3.98 FAQGATSWGLMEE495 pKa = 4.22 EE496 pKa = 5.11 PIWQDD501 pKa = 3.16 FVKK504 pKa = 10.72 LSEE507 pKa = 4.23 IYY509 pKa = 9.71 TLEE512 pKa = 3.75 KK513 pKa = 10.46 TEE515 pKa = 4.09 EE516 pKa = 3.99 KK517 pKa = 10.7 KK518 pKa = 10.99 KK519 pKa = 9.57 FDD521 pKa = 3.04 RR522 pKa = 11.84 MKK524 pKa = 10.64 RR525 pKa = 11.84 IRR527 pKa = 11.84 QLHH530 pKa = 5.73 KK531 pKa = 10.92 KK532 pKa = 8.86 RR533 pKa = 11.84 MKK535 pKa = 10.59 YY536 pKa = 9.61 YY537 pKa = 10.72 AA538 pKa = 4.15
Molecular weight: 64.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.748
IPC2_protein 8.726
IPC_protein 8.551
Toseland 9.282
ProMoST 9.107
Dawson 9.589
Bjellqvist 9.355
Wikipedia 9.75
Rodwell 9.867
Grimsley 9.648
Solomon 9.604
Lehninger 9.56
Nozaki 9.472
DTASelect 9.297
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.56
Patrickios 4.584
IPC_peptide 9.589
IPC2_peptide 7.966
IPC2.peptide.svr19 7.789
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1725
94
617
345.0
39.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.246 ± 1.83
1.681 ± 0.59
4.696 ± 0.585
8.348 ± 1.092
4.406 ± 0.799
6.261 ± 0.78
1.159 ± 0.444
7.826 ± 0.36
9.159 ± 2.598
6.087 ± 0.469
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.536 ± 0.409
7.536 ± 0.916
2.725 ± 0.51
5.043 ± 1.037
4.232 ± 0.461
4.464 ± 1.058
6.029 ± 0.411
3.246 ± 0.326
1.681 ± 0.309
4.638 ± 1.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here