Amino acid dipepetide frequency for Flavobacterium sp. ALD4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.846AlaAla: 4.846 ± 0.095
0.528AlaCys: 0.528 ± 0.025
3.459AlaAsp: 3.459 ± 0.079
3.978AlaGlu: 3.978 ± 0.067
3.389AlaPhe: 3.389 ± 0.064
4.386AlaGly: 4.386 ± 0.095
1.036AlaHis: 1.036 ± 0.037
6.055AlaIle: 6.055 ± 0.09
5.053AlaLys: 5.053 ± 0.103
6.601AlaLeu: 6.601 ± 0.099
1.688AlaMet: 1.688 ± 0.05
3.552AlaAsn: 3.552 ± 0.08
1.965AlaPro: 1.965 ± 0.049
2.433AlaGln: 2.433 ± 0.062
1.963AlaArg: 1.963 ± 0.054
4.386AlaSer: 4.386 ± 0.072
4.148AlaThr: 4.148 ± 0.084
4.636AlaVal: 4.636 ± 0.078
0.578AlaTrp: 0.578 ± 0.027
2.315AlaTyr: 2.315 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.464CysAla: 0.464 ± 0.024
0.087CysCys: 0.087 ± 0.009
0.413CysAsp: 0.413 ± 0.02
0.428CysGlu: 0.428 ± 0.023
0.371CysPhe: 0.371 ± 0.02
0.617CysGly: 0.617 ± 0.027
0.187CysHis: 0.187 ± 0.017
0.564CysIle: 0.564 ± 0.027
0.485CysLys: 0.485 ± 0.022
0.628CysLeu: 0.628 ± 0.025
0.138CysMet: 0.138 ± 0.013
0.391CysAsn: 0.391 ± 0.02
0.327CysPro: 0.327 ± 0.022
0.207CysGln: 0.207 ± 0.017
0.201CysArg: 0.201 ± 0.013
0.544CysSer: 0.544 ± 0.025
0.414CysThr: 0.414 ± 0.023
0.428CysVal: 0.428 ± 0.024
0.06CysTrp: 0.06 ± 0.008
0.284CysTyr: 0.284 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.799AspAla: 3.799 ± 0.072
0.39AspCys: 0.39 ± 0.021
2.543AspAsp: 2.543 ± 0.067
3.38AspGlu: 3.38 ± 0.07
3.554AspPhe: 3.554 ± 0.067
3.419AspGly: 3.419 ± 0.067
0.793AspHis: 0.793 ± 0.033
4.309AspIle: 4.309 ± 0.074
4.378AspLys: 4.378 ± 0.083
5.232AspLeu: 5.232 ± 0.08
1.127AspMet: 1.127 ± 0.036
3.022AspAsn: 3.022 ± 0.063
1.592AspPro: 1.592 ± 0.043
1.493AspGln: 1.493 ± 0.041
1.735AspArg: 1.735 ± 0.047
3.191AspSer: 3.191 ± 0.059
2.695AspThr: 2.695 ± 0.057
3.516AspVal: 3.516 ± 0.066
0.655AspTrp: 0.655 ± 0.027
2.694AspTyr: 2.694 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
4.292GluAla: 4.292 ± 0.087
0.321GluCys: 0.321 ± 0.02
3.206GluAsp: 3.206 ± 0.067
4.553GluGlu: 4.553 ± 0.089
2.825GluPhe: 2.825 ± 0.06
3.643GluGly: 3.643 ± 0.066
1.02GluHis: 1.02 ± 0.035
5.661GluIle: 5.661 ± 0.086
6.006GluLys: 6.006 ± 0.094
5.978GluLeu: 5.978 ± 0.088
1.765GluMet: 1.765 ± 0.049
4.566GluAsn: 4.566 ± 0.078
1.467GluPro: 1.467 ± 0.042
2.085GluGln: 2.085 ± 0.052
2.36GluArg: 2.36 ± 0.055
3.407GluSer: 3.407 ± 0.063
3.417GluThr: 3.417 ± 0.06
4.173GluVal: 4.173 ± 0.078
0.537GluTrp: 0.537 ± 0.026
2.328GluTyr: 2.328 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.346PheAla: 3.346 ± 0.064
0.434PheCys: 0.434 ± 0.02
3.257PheAsp: 3.257 ± 0.069
3.426PheGlu: 3.426 ± 0.053
2.896PhePhe: 2.896 ± 0.074
3.74PheGly: 3.74 ± 0.072
0.879PheHis: 0.879 ± 0.033
4.161PheIle: 4.161 ± 0.088
3.737PheLys: 3.737 ± 0.077
4.785PheLeu: 4.785 ± 0.084
1.19PheMet: 1.19 ± 0.039
3.102PheAsn: 3.102 ± 0.067
1.733PhePro: 1.733 ± 0.048
1.547PheGln: 1.547 ± 0.04
1.552PheArg: 1.552 ± 0.039
4.042PheSer: 4.042 ± 0.083
3.117PheThr: 3.117 ± 0.062
3.216PheVal: 3.216 ± 0.059
0.508PheTrp: 0.508 ± 0.024
2.201PheTyr: 2.201 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
4.329GlyAla: 4.329 ± 0.082
0.607GlyCys: 0.607 ± 0.028
3.173GlyAsp: 3.173 ± 0.064
3.383GlyGlu: 3.383 ± 0.062
3.839GlyPhe: 3.839 ± 0.072
4.553GlyGly: 4.553 ± 0.1
1.105GlyHis: 1.105 ± 0.034
5.663GlyIle: 5.663 ± 0.088
5.089GlyLys: 5.089 ± 0.077
5.82GlyLeu: 5.82 ± 0.09
1.748GlyMet: 1.748 ± 0.044
3.531GlyAsn: 3.531 ± 0.081
1.341GlyPro: 1.341 ± 0.039
1.872GlyGln: 1.872 ± 0.048
2.039GlyArg: 2.039 ± 0.05
4.114GlySer: 4.114 ± 0.078
4.132GlyThr: 4.132 ± 0.062
4.423GlyVal: 4.423 ± 0.083
0.689GlyTrp: 0.689 ± 0.033
2.771GlyTyr: 2.771 ± 0.066
0.0GlyXaa: 0.0 ± 0.0
His
0.947HisAla: 0.947 ± 0.035
0.157HisCys: 0.157 ± 0.015
0.806HisAsp: 0.806 ± 0.029
0.932HisGlu: 0.932 ± 0.036
1.149HisPhe: 1.149 ± 0.04
0.99HisGly: 0.99 ± 0.032
0.461HisHis: 0.461 ± 0.024
1.332HisIle: 1.332 ± 0.04
1.119HisLys: 1.119 ± 0.037
1.688HisLeu: 1.688 ± 0.046
0.325HisMet: 0.325 ± 0.018
0.872HisAsn: 0.872 ± 0.032
0.826HisPro: 0.826 ± 0.033
0.706HisGln: 0.706 ± 0.034
0.593HisArg: 0.593 ± 0.024
1.016HisSer: 1.016 ± 0.037
0.848HisThr: 0.848 ± 0.03
0.875HisVal: 0.875 ± 0.033
0.177HisTrp: 0.177 ± 0.014
0.794HisTyr: 0.794 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.262IleAla: 6.262 ± 0.098
0.634IleCys: 0.634 ± 0.028
4.908IleAsp: 4.908 ± 0.075
5.541IleGlu: 5.541 ± 0.103
3.701IlePhe: 3.701 ± 0.078
5.467IleGly: 5.467 ± 0.095
1.369IleHis: 1.369 ± 0.045
6.827IleIle: 6.827 ± 0.119
6.118IleLys: 6.118 ± 0.088
7.494IleLeu: 7.494 ± 0.113
1.637IleMet: 1.637 ± 0.041
4.677IleAsn: 4.677 ± 0.08
3.414IlePro: 3.414 ± 0.06
2.772IleGln: 2.772 ± 0.061
2.601IleArg: 2.601 ± 0.057
5.778IleSer: 5.778 ± 0.09
5.027IleThr: 5.027 ± 0.072
5.384IleVal: 5.384 ± 0.081
0.678IleTrp: 0.678 ± 0.03
2.752IleTyr: 2.752 ± 0.052
0.0IleXaa: 0.0 ± 0.0
Lys
5.08LysAla: 5.08 ± 0.097
0.381LysCys: 0.381 ± 0.02
4.232LysAsp: 4.232 ± 0.07
6.094LysGlu: 6.094 ± 0.093
2.997LysPhe: 2.997 ± 0.059
4.629LysGly: 4.629 ± 0.076
1.186LysHis: 1.186 ± 0.04
6.925LysIle: 6.925 ± 0.098
6.888LysLys: 6.888 ± 0.114
6.599LysLeu: 6.599 ± 0.097
2.353LysMet: 2.353 ± 0.053
5.375LysAsn: 5.375 ± 0.088
2.44LysPro: 2.44 ± 0.042
2.661LysGln: 2.661 ± 0.057
2.663LysArg: 2.663 ± 0.052
4.739LysSer: 4.739 ± 0.074
4.837LysThr: 4.837 ± 0.076
4.95LysVal: 4.95 ± 0.094
0.756LysTrp: 0.756 ± 0.033
3.129LysTyr: 3.129 ± 0.059
0.0LysXaa: 0.0 ± 0.0
Leu
6.14LeuAla: 6.14 ± 0.085
0.653LeuCys: 0.653 ± 0.029
5.052LeuAsp: 5.052 ± 0.082
5.929LeuGlu: 5.929 ± 0.08
5.209LeuPhe: 5.209 ± 0.096
5.905LeuGly: 5.905 ± 0.1
1.557LeuHis: 1.557 ± 0.041
7.254LeuIle: 7.254 ± 0.103
7.583LeuLys: 7.583 ± 0.096
9.044LeuLeu: 9.044 ± 0.138
2.228LeuMet: 2.228 ± 0.05
5.553LeuAsn: 5.553 ± 0.084
3.371LeuPro: 3.371 ± 0.061
3.118LeuGln: 3.118 ± 0.067
3.075LeuArg: 3.075 ± 0.068
6.816LeuSer: 6.816 ± 0.105
5.151LeuThr: 5.151 ± 0.079
5.54LeuVal: 5.54 ± 0.088
0.749LeuTrp: 0.749 ± 0.031
3.177LeuTyr: 3.177 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
1.726MetAla: 1.726 ± 0.046
0.134MetCys: 0.134 ± 0.012
1.23MetAsp: 1.23 ± 0.036
1.516MetGlu: 1.516 ± 0.042
0.875MetPhe: 0.875 ± 0.033
1.541MetGly: 1.541 ± 0.044
0.409MetHis: 0.409 ± 0.023
1.795MetIle: 1.795 ± 0.05
2.416MetLys: 2.416 ± 0.056
2.023MetLeu: 2.023 ± 0.057
0.694MetMet: 0.694 ± 0.041
1.483MetAsn: 1.483 ± 0.038
0.872MetPro: 0.872 ± 0.033
0.87MetGln: 0.87 ± 0.031
0.85MetArg: 0.85 ± 0.034
1.54MetSer: 1.54 ± 0.045
1.301MetThr: 1.301 ± 0.041
1.414MetVal: 1.414 ± 0.037
0.172MetTrp: 0.172 ± 0.013
0.756MetTyr: 0.756 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.883AsnAla: 3.883 ± 0.067
0.426AsnCys: 0.426 ± 0.023
3.035AsnAsp: 3.035 ± 0.06
3.723AsnGlu: 3.723 ± 0.07
2.964AsnPhe: 2.964 ± 0.072
3.9AsnGly: 3.9 ± 0.069
0.989AsnHis: 0.989 ± 0.033
4.674AsnIle: 4.674 ± 0.069
4.55AsnLys: 4.55 ± 0.078
5.277AsnLeu: 5.277 ± 0.076
1.279AsnMet: 1.279 ± 0.039
3.663AsnAsn: 3.663 ± 0.082
2.708AsnPro: 2.708 ± 0.062
2.154AsnGln: 2.154 ± 0.051
2.105AsnArg: 2.105 ± 0.048
4.016AsnSer: 4.016 ± 0.094
3.604AsnThr: 3.604 ± 0.07
3.535AsnVal: 3.535 ± 0.071
0.759AsnTrp: 0.759 ± 0.034
2.811AsnTyr: 2.811 ± 0.07
0.0AsnXaa: 0.0 ± 0.0
Pro
2.076ProAla: 2.076 ± 0.046
0.193ProCys: 0.193 ± 0.016
1.851ProAsp: 1.851 ± 0.049
2.562ProGlu: 2.562 ± 0.059
1.998ProPhe: 1.998 ± 0.055
1.811ProGly: 1.811 ± 0.053
0.548ProHis: 0.548 ± 0.022
2.794ProIle: 2.794 ± 0.061
2.529ProLys: 2.529 ± 0.051
2.995ProLeu: 2.995 ± 0.065
0.792ProMet: 0.792 ± 0.031
2.086ProAsn: 2.086 ± 0.052
0.722ProPro: 0.722 ± 0.028
1.054ProGln: 1.054 ± 0.034
0.894ProArg: 0.894 ± 0.03
2.166ProSer: 2.166 ± 0.054
2.076ProThr: 2.076 ± 0.051
2.32ProVal: 2.32 ± 0.053
0.326ProTrp: 0.326 ± 0.019
1.363ProTyr: 1.363 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.023GlnAla: 2.023 ± 0.053
0.181GlnCys: 0.181 ± 0.015
1.528GlnAsp: 1.528 ± 0.04
2.186GlnGlu: 2.186 ± 0.052
1.751GlnPhe: 1.751 ± 0.044
1.724GlnGly: 1.724 ± 0.041
0.552GlnHis: 0.552 ± 0.024
2.742GlnIle: 2.742 ± 0.06
2.971GlnLys: 2.971 ± 0.061
3.419GlnLeu: 3.419 ± 0.064
0.823GlnMet: 0.823 ± 0.027
2.2GlnAsn: 2.2 ± 0.06
0.927GlnPro: 0.927 ± 0.033
1.261GlnGln: 1.261 ± 0.049
1.113GlnArg: 1.113 ± 0.04
2.033GlnSer: 2.033 ± 0.049
1.89GlnThr: 1.89 ± 0.051
2.012GlnVal: 2.012 ± 0.047
0.343GlnTrp: 0.343 ± 0.02
1.276GlnTyr: 1.276 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
2.048ArgAla: 2.048 ± 0.053
0.216ArgCys: 0.216 ± 0.017
1.744ArgAsp: 1.744 ± 0.045
2.12ArgGlu: 2.12 ± 0.06
1.768ArgPhe: 1.768 ± 0.046
1.833ArgGly: 1.833 ± 0.048
0.544ArgHis: 0.544 ± 0.023
2.777ArgIle: 2.777 ± 0.061
2.727ArgLys: 2.727 ± 0.055
3.096ArgLeu: 3.096 ± 0.06
0.949ArgMet: 0.949 ± 0.031
1.983ArgAsn: 1.983 ± 0.052
0.98ArgPro: 0.98 ± 0.032
0.968ArgGln: 0.968 ± 0.035
1.189ArgArg: 1.189 ± 0.04
1.881ArgSer: 1.881 ± 0.049
1.813ArgThr: 1.813 ± 0.054
2.082ArgVal: 2.082 ± 0.055
0.332ArgTrp: 0.332 ± 0.017
1.413ArgTyr: 1.413 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
3.999SerAla: 3.999 ± 0.07
0.607SerCys: 0.607 ± 0.028
3.497SerAsp: 3.497 ± 0.065
3.731SerGlu: 3.731 ± 0.073
3.981SerPhe: 3.981 ± 0.068
4.664SerGly: 4.664 ± 0.084
1.183SerHis: 1.183 ± 0.037
5.416SerIle: 5.416 ± 0.082
5.012SerLys: 5.012 ± 0.076
6.013SerLeu: 6.013 ± 0.099
1.343SerMet: 1.343 ± 0.04
3.82SerAsn: 3.82 ± 0.082
2.126SerPro: 2.126 ± 0.05
2.216SerGln: 2.216 ± 0.048
2.169SerArg: 2.169 ± 0.048
4.399SerSer: 4.399 ± 0.1
3.768SerThr: 3.768 ± 0.079
4.224SerVal: 4.224 ± 0.076
0.66SerTrp: 0.66 ± 0.026
2.819SerTyr: 2.819 ± 0.059
0.0SerXaa: 0.0 ± 0.0
Thr
4.199ThrAla: 4.199 ± 0.089
0.337ThrCys: 0.337 ± 0.02
3.245ThrAsp: 3.245 ± 0.069
3.479ThrGlu: 3.479 ± 0.065
3.029ThrPhe: 3.029 ± 0.056
4.093ThrGly: 4.093 ± 0.066
0.913ThrHis: 0.913 ± 0.034
5.363ThrIle: 5.363 ± 0.086
4.109ThrLys: 4.109 ± 0.071
5.328ThrLeu: 5.328 ± 0.085
1.092ThrMet: 1.092 ± 0.035
3.431ThrAsn: 3.431 ± 0.075
2.38ThrPro: 2.38 ± 0.053
1.861ThrGln: 1.861 ± 0.051
1.58ThrArg: 1.58 ± 0.043
3.657ThrSer: 3.657 ± 0.068
3.801ThrThr: 3.801 ± 0.085
4.075ThrVal: 4.075 ± 0.07
0.501ThrTrp: 0.501 ± 0.023
2.188ThrTyr: 2.188 ± 0.053
0.0ThrXaa: 0.0 ± 0.0
Val
4.555ValAla: 4.555 ± 0.085
0.506ValCys: 0.506 ± 0.023
3.52ValAsp: 3.52 ± 0.057
3.815ValGlu: 3.815 ± 0.076
3.609ValPhe: 3.609 ± 0.071
4.037ValGly: 4.037 ± 0.07
0.966ValHis: 0.966 ± 0.038
5.23ValIle: 5.23 ± 0.081
4.467ValLys: 4.467 ± 0.079
6.431ValLeu: 6.431 ± 0.094
1.419ValMet: 1.419 ± 0.044
3.617ValAsn: 3.617 ± 0.066
2.261ValPro: 2.261 ± 0.054
1.873ValGln: 1.873 ± 0.056
1.989ValArg: 1.989 ± 0.045
4.559ValSer: 4.559 ± 0.068
3.812ValThr: 3.812 ± 0.08
4.58ValVal: 4.58 ± 0.082
0.564ValTrp: 0.564 ± 0.027
2.333ValTyr: 2.333 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
0.578TrpAla: 0.578 ± 0.026
0.091TrpCys: 0.091 ± 0.009
0.545TrpAsp: 0.545 ± 0.029
0.585TrpGlu: 0.585 ± 0.028
0.518TrpPhe: 0.518 ± 0.024
0.611TrpGly: 0.611 ± 0.029
0.204TrpHis: 0.204 ± 0.016
0.709TrpIle: 0.709 ± 0.031
0.735TrpLys: 0.735 ± 0.028
0.897TrpLeu: 0.897 ± 0.035
0.287TrpMet: 0.287 ± 0.017
0.673TrpAsn: 0.673 ± 0.03
0.212TrpPro: 0.212 ± 0.017
0.358TrpGln: 0.358 ± 0.019
0.333TrpArg: 0.333 ± 0.019
0.574TrpSer: 0.574 ± 0.026
0.545TrpThr: 0.545 ± 0.021
0.56TrpVal: 0.56 ± 0.023
0.119TrpTrp: 0.119 ± 0.012
0.43TrpTyr: 0.43 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.406TyrAla: 2.406 ± 0.046
0.353TyrCys: 0.353 ± 0.024
2.271TyrAsp: 2.271 ± 0.051
2.25TyrGlu: 2.25 ± 0.046
2.446TyrPhe: 2.446 ± 0.055
2.658TyrGly: 2.658 ± 0.065
0.711TyrHis: 0.711 ± 0.027
2.791TyrIle: 2.791 ± 0.055
2.941TyrLys: 2.941 ± 0.06
3.758TyrLeu: 3.758 ± 0.06
0.736TyrMet: 0.736 ± 0.03
2.461TyrAsn: 2.461 ± 0.06
1.492TyrPro: 1.492 ± 0.042
1.443TyrGln: 1.443 ± 0.042
1.459TyrArg: 1.459 ± 0.036
2.787TyrSer: 2.787 ± 0.053
2.264TyrThr: 2.264 ± 0.061
2.2TyrVal: 2.2 ± 0.048
0.404TyrTrp: 0.404 ± 0.022
1.79TyrTyr: 1.79 ± 0.042
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2796 proteins (921015 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski