Proteus phage PM135

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Novosibvirus; Proteus virus PM135

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PRQ4|A0A2H4PRQ4_9CAUD Uncharacterized protein OS=Proteus phage PM135 OX=2048008 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.29HH3 pKa = 5.15KK4 pKa = 10.18QAYY7 pKa = 9.48LCEE10 pKa = 4.62CGALSDD16 pKa = 4.16TQDD19 pKa = 3.11NCPDD23 pKa = 4.3CGNDD27 pKa = 3.65DD28 pKa = 4.18LSDD31 pKa = 3.16ISVYY35 pKa = 11.0LKK37 pKa = 9.11FTIFDD42 pKa = 3.97ADD44 pKa = 3.58SCNSLEE50 pKa = 4.45VYY52 pKa = 10.25IDD54 pKa = 3.52TNMCLEE60 pKa = 4.25DD61 pKa = 3.83WNNLSDD67 pKa = 3.6TDD69 pKa = 4.45KK70 pKa = 9.99IQHH73 pKa = 5.01VAEE76 pKa = 4.83CKK78 pKa = 9.12EE79 pKa = 3.7QLLIRR84 pKa = 11.84SEE86 pKa = 4.42VIAVDD91 pKa = 3.37NDD93 pKa = 3.66GNDD96 pKa = 3.24MFEE99 pKa = 4.0VV100 pKa = 3.67

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PRI6|A0A2H4PRI6_9CAUD Uncharacterized protein OS=Proteus phage PM135 OX=2048008 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.25SRR4 pKa = 11.84KK5 pKa = 9.4GLEE8 pKa = 3.7LPAYY12 pKa = 9.35IMRR15 pKa = 11.84GFVDD19 pKa = 4.79KK20 pKa = 9.99FTEE23 pKa = 4.36DD24 pKa = 3.28GFVIIQSHH32 pKa = 5.08LTRR35 pKa = 11.84YY36 pKa = 9.04VFDD39 pKa = 4.05MPSLPGNYY47 pKa = 9.8AKK49 pKa = 10.39RR50 pKa = 11.84RR51 pKa = 11.84LILSSMKK58 pKa = 10.31LPYY61 pKa = 10.14KK62 pKa = 10.53LYY64 pKa = 9.68PLQKK68 pKa = 10.09RR69 pKa = 11.84ITTISQFIRR78 pKa = 11.84SGAKK82 pKa = 10.23YY83 pKa = 10.21IITPDD88 pKa = 2.92GDD90 pKa = 3.0IRR92 pKa = 11.84RR93 pKa = 11.84VRR95 pKa = 11.84RR96 pKa = 11.84TKK98 pKa = 10.4FYY100 pKa = 9.15PIEE103 pKa = 4.12VKK105 pKa = 10.33RR106 pKa = 11.84VSISNQTFNGTYY118 pKa = 9.41QNFVKK123 pKa = 10.8GSLSPIITDD132 pKa = 4.27CEE134 pKa = 3.73AKK136 pKa = 10.56YY137 pKa = 10.62ISVVNYY143 pKa = 10.18NGALVVFDD151 pKa = 3.85VHH153 pKa = 8.38RR154 pKa = 11.84EE155 pKa = 3.89YY156 pKa = 10.77PEE158 pKa = 3.64TRR160 pKa = 11.84RR161 pKa = 11.84YY162 pKa = 9.16RR163 pKa = 11.84VKK165 pKa = 10.88LL166 pKa = 3.46

Molecular weight: 19.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

24893

49

1232

244.0

27.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.135 ± 0.389

1.012 ± 0.102

6.259 ± 0.161

7.127 ± 0.248

4.009 ± 0.142

6.383 ± 0.233

1.876 ± 0.144

6.713 ± 0.187

6.918 ± 0.21

8.846 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.135

5.25 ± 0.171

3.776 ± 0.174

3.242 ± 0.2

4.351 ± 0.172

7.006 ± 0.23

6.058 ± 0.203

6.58 ± 0.161

1.061 ± 0.104

3.905 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski