Phenylobacterium hankyongense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Phenylobacterium

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3638 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328B3P8|A0A328B3P8_9CAUL GNAT family N-acetyltransferase OS=Phenylobacterium hankyongense OX=1813876 GN=DJ021_11940 PE=4 SV=1
MM1 pKa = 7.31PTLTFDD7 pKa = 3.39TTPAGLFASYY17 pKa = 11.02GEE19 pKa = 4.39SQFTLTTQQATDD31 pKa = 3.32LHH33 pKa = 6.01LTDD36 pKa = 4.42VLWNGGLTSPQGTGGYY52 pKa = 9.87LINEE56 pKa = 4.41YY57 pKa = 10.84SQDD60 pKa = 3.72TVVLKK65 pKa = 10.68YY66 pKa = 10.88SGTTTFGVLSLDD78 pKa = 3.46VNGYY82 pKa = 10.22RR83 pKa = 11.84NAGFDD88 pKa = 3.65ANNNVIPGSSTVSFNFTGVRR108 pKa = 11.84ADD110 pKa = 3.4YY111 pKa = 10.74TEE113 pKa = 3.75VHH115 pKa = 5.69FTFTTDD121 pKa = 3.35AVDD124 pKa = 4.29GFQQVVLPTDD134 pKa = 4.31FATGLTALTWTTTNPVEE151 pKa = 3.85AWGAFDD157 pKa = 4.42NVSLVVDD164 pKa = 4.33TTQAPPPVTPPPVTPPPVNHH184 pKa = 6.92APVAVQDD191 pKa = 3.67VAAATVHH198 pKa = 5.23GTVLINVLGNDD209 pKa = 3.3TDD211 pKa = 4.65ADD213 pKa = 4.15GDD215 pKa = 4.07TLGIAGFGPSASAISASLSAKK236 pKa = 9.74SALGATISFQSGQLLYY252 pKa = 9.62TANAVSFDD260 pKa = 3.42AVAPGHH266 pKa = 6.07SVTDD270 pKa = 3.25TFYY273 pKa = 10.97YY274 pKa = 10.14TVTDD278 pKa = 3.62AAGATSTASVTVTVSSPPPPPAPPPSATGPDD309 pKa = 3.33IVGGNHH315 pKa = 5.62PQVLNGTALGEE326 pKa = 4.22QIFGGNSSDD335 pKa = 3.42VLMGNGGADD344 pKa = 3.29TLHH347 pKa = 6.58GNNGADD353 pKa = 3.95LLYY356 pKa = 11.08GGAGNDD362 pKa = 4.71LLLGDD367 pKa = 4.77NGSDD371 pKa = 4.35LLDD374 pKa = 4.1GGAGNDD380 pKa = 3.59TLTGGNSPDD389 pKa = 3.18AFVFSQHH396 pKa = 6.18FGLDD400 pKa = 3.49TITDD404 pKa = 4.16FDD406 pKa = 4.53SKK408 pKa = 11.62NEE410 pKa = 4.14VITMSRR416 pKa = 11.84STFSSFNDD424 pKa = 3.68LKK426 pKa = 11.32AHH428 pKa = 6.76AVQSGANVVITHH440 pKa = 7.14DD441 pKa = 3.81ADD443 pKa = 3.89EE444 pKa = 4.66VLTLLNVKK452 pKa = 10.34LSALNSGDD460 pKa = 3.47FLFVV464 pKa = 3.11

Molecular weight: 47.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328B0B2|A0A328B0B2_9CAUL Pyridoxal phosphate homeostasis protein OS=Phenylobacterium hankyongense OX=1813876 GN=DJ021_05875 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.47GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTKK25 pKa = 10.17NGQKK29 pKa = 9.5VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3638

0

3638

1152277

28

2560

316.7

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.268 ± 0.066

0.73 ± 0.012

5.669 ± 0.031

5.459 ± 0.043

3.46 ± 0.022

9.085 ± 0.051

1.883 ± 0.019

4.095 ± 0.029

2.895 ± 0.032

10.046 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.02

2.283 ± 0.026

5.758 ± 0.037

3.296 ± 0.03

7.521 ± 0.052

4.919 ± 0.035

5.229 ± 0.06

7.662 ± 0.034

1.369 ± 0.015

2.183 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski