Hydrogenothermus marinus

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Hydrogenothermaceae; Hydrogenothermus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M0BL79|A0A3M0BL79_9AQUI Protein TonB OS=Hydrogenothermus marinus OX=133270 GN=CLV39_0850 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.23LKK4 pKa = 11.06VMVDD8 pKa = 3.15QDD10 pKa = 4.05LCTACALCYY19 pKa = 10.63DD20 pKa = 4.81DD21 pKa = 5.52VPEE24 pKa = 4.35VYY26 pKa = 10.19EE27 pKa = 4.56DD28 pKa = 3.9AGDD31 pKa = 4.67GIAKK35 pKa = 10.28VKK37 pKa = 11.08DD38 pKa = 3.55EE39 pKa = 4.35VGGDD43 pKa = 3.73GAILDD48 pKa = 4.4CEE50 pKa = 4.33EE51 pKa = 4.45VGQDD55 pKa = 2.92LCDD58 pKa = 3.33RR59 pKa = 11.84VLEE62 pKa = 4.28VTEE65 pKa = 4.36EE66 pKa = 4.32CPSGSLITEE75 pKa = 4.28VVEE78 pKa = 4.32EE79 pKa = 4.22

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M0BQJ2|A0A3M0BQJ2_9AQUI Dihydropteroate synthase OS=Hydrogenothermus marinus OX=133270 GN=CLV39_1227 PE=4 SV=1
MM1 pKa = 7.75AEE3 pKa = 4.62KK4 pKa = 10.19IHH6 pKa = 6.76NNLSNKK12 pKa = 8.48KK13 pKa = 9.31RR14 pKa = 11.84KK15 pKa = 8.23RR16 pKa = 11.84TSGFLARR23 pKa = 11.84KK24 pKa = 6.14RR25 pKa = 11.84TKK27 pKa = 10.11SGRR30 pKa = 11.84KK31 pKa = 7.74ILARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.0GRR41 pKa = 11.84KK42 pKa = 8.71RR43 pKa = 11.84LAVV46 pKa = 3.41

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1621

0

1621

488413

30

1602

301.3

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.615 ± 0.061

0.653 ± 0.02

5.192 ± 0.04

8.271 ± 0.073

5.149 ± 0.06

5.891 ± 0.059

1.354 ± 0.024

10.427 ± 0.076

11.064 ± 0.089

9.571 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.783 ± 0.024

5.351 ± 0.064

3.559 ± 0.036

2.367 ± 0.031

3.269 ± 0.038

5.095 ± 0.042

4.33 ± 0.037

5.901 ± 0.049

0.799 ± 0.022

4.36 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski