Streptococcus phage Javan163

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A0Z3|A0A4D6A0Z3_9CAUD Uncharacterized protein OS=Streptococcus phage Javan163 OX=2548015 GN=Javan163_0050 PE=4 SV=1
MM1 pKa = 7.64NYY3 pKa = 10.44LEE5 pKa = 4.33YY6 pKa = 11.13ALAYY10 pKa = 10.27LEE12 pKa = 4.77RR13 pKa = 11.84EE14 pKa = 4.2LEE16 pKa = 4.54IIDD19 pKa = 4.43DD20 pKa = 3.92EE21 pKa = 4.88VIEE24 pKa = 4.2VEE26 pKa = 4.76LPGGDD31 pKa = 3.23WEE33 pKa = 4.69FVPNPYY39 pKa = 10.03YY40 pKa = 10.52EE41 pKa = 4.88EE42 pKa = 4.59GLHH45 pKa = 7.02DD46 pKa = 4.16SPHH49 pKa = 5.84YY50 pKa = 9.94RR51 pKa = 11.84SQVAKK56 pKa = 10.58DD57 pKa = 3.09ILDD60 pKa = 3.46IKK62 pKa = 10.8GLLGRR67 pKa = 4.72

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A0Y4|A0A4D6A0Y4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan163 OX=2548015 GN=Javan163_0040 PE=4 SV=1
MM1 pKa = 7.01QSNVIAVLMTAVIMLLMISIALQNHH26 pKa = 6.06YY27 pKa = 10.12EE28 pKa = 3.97PQITGLRR35 pKa = 11.84TQLSRR40 pKa = 11.84TQKK43 pKa = 8.22QLKK46 pKa = 9.29RR47 pKa = 11.84ASEE50 pKa = 3.82DD51 pKa = 2.95RR52 pKa = 11.84ARR54 pKa = 11.84QTKK57 pKa = 10.13RR58 pKa = 11.84IAEE61 pKa = 4.08LTGNGGG67 pKa = 3.26

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12508

38

1460

240.5

26.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.099 ± 0.653

0.424 ± 0.082

6.22 ± 0.489

7.291 ± 0.616

3.646 ± 0.219

6.444 ± 0.408

1.327 ± 0.158

6.956 ± 0.295

8.594 ± 0.589

8.674 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.178

5.317 ± 0.286

2.702 ± 0.158

3.909 ± 0.311

4.237 ± 0.334

6.628 ± 0.71

6.084 ± 0.298

6.18 ± 0.223

1.103 ± 0.113

3.838 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski