Pantoea phage vB_PagM_AAM22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A513ZY79|A0A513ZY79_9CAUD Uncharacterized protein OS=Pantoea phage vB_PagM_AAM22 OX=2588092 GN=AAM22_gp94 PE=4 SV=1
MM1 pKa = 7.87QEE3 pKa = 3.97IPLSNGPANAHH14 pKa = 4.99QQFSLQLGDD23 pKa = 3.8NYY25 pKa = 11.4LDD27 pKa = 3.98FEE29 pKa = 5.0VNYY32 pKa = 9.97ISYY35 pKa = 9.69TDD37 pKa = 3.6TPAWSLDD44 pKa = 2.93IYY46 pKa = 11.09RR47 pKa = 11.84DD48 pKa = 3.64GTPLVLGAMLVPGADD63 pKa = 3.15VTNGYY68 pKa = 9.4RR69 pKa = 11.84ADD71 pKa = 3.12IGRR74 pKa = 11.84FVFVGDD80 pKa = 4.3EE81 pKa = 4.13VTLDD85 pKa = 3.5NLGLNNHH92 pKa = 6.26LVWVSEE98 pKa = 4.08

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A513ZY95|A0A513ZY95_9CAUD DNA-directed DNA polymerase OS=Pantoea phage vB_PagM_AAM22 OX=2588092 GN=AAM22_gp57 PE=4 SV=1
MM1 pKa = 7.03RR2 pKa = 11.84QAWTTSEE9 pKa = 3.98QRR11 pKa = 11.84WLAGLAPTHH20 pKa = 5.84TVAEE24 pKa = 4.25LSEE27 pKa = 4.0KK28 pKa = 10.87LEE30 pKa = 4.05RR31 pKa = 11.84SKK33 pKa = 11.4GAIVSQLNLIGITAKK48 pKa = 10.03PQNKK52 pKa = 8.34AWMQRR57 pKa = 11.84EE58 pKa = 4.11VKK60 pKa = 9.82IVQEE64 pKa = 4.03MMHH67 pKa = 6.95DD68 pKa = 4.11FTYY71 pKa = 11.12EE72 pKa = 3.76EE73 pKa = 4.28IARR76 pKa = 11.84EE77 pKa = 4.15LGRR80 pKa = 11.84SSASVRR86 pKa = 11.84AYY88 pKa = 10.54VRR90 pKa = 11.84RR91 pKa = 11.84CCNPEE96 pKa = 3.91CRR98 pKa = 11.84KK99 pKa = 8.84HH100 pKa = 4.32TWEE103 pKa = 3.69PTRR106 pKa = 11.84RR107 pKa = 11.84EE108 pKa = 3.57IARR111 pKa = 11.84IARR114 pKa = 11.84LRR116 pKa = 11.84KK117 pKa = 9.69NKK119 pKa = 9.77VSLPVIAEE127 pKa = 4.0KK128 pKa = 10.76LGCTRR133 pKa = 11.84RR134 pKa = 11.84NLEE137 pKa = 4.29NVIKK141 pKa = 10.5QNNIRR146 pKa = 11.84RR147 pKa = 11.84GIGYY151 pKa = 9.14VRR153 pKa = 3.96

Molecular weight: 17.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

15812

21

752

164.7

18.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.816 ± 0.31

1.341 ± 0.149

6.141 ± 0.285

5.78 ± 0.269

3.377 ± 0.176

7.988 ± 0.489

1.859 ± 0.184

5.578 ± 0.179

5.249 ± 0.231

7.368 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.148

4.813 ± 0.194

3.965 ± 0.261

4.364 ± 0.225

5.578 ± 0.261

6.261 ± 0.237

6.552 ± 0.294

7.241 ± 0.22

1.701 ± 0.112

3.251 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski