Cotton leaf curl Multan betasatellite
Average proteome isoelectric point is 8.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1XJC1|M1XJC1_9VIRU Truncated beta C1 protein OS=Cotton leaf curl Multan betasatellite OX=306025 GN=C1 PE=4 SV=1
MM1 pKa = 7.17 VKK3 pKa = 9.96 PRR5 pKa = 11.84 SHH7 pKa = 6.72 LQTTTFSDD15 pKa = 3.39 MFLFCLFFYY24 pKa = 10.72 YY25 pKa = 10.28 SQTMTFSNLYY35 pKa = 9.48 FFLFTFYY42 pKa = 10.61 FFSFLGFSCFDD53 pKa = 3.38 SLRR56 pKa = 11.84 SHH58 pKa = 7.51 FFHH61 pKa = 7.66 KK62 pKa = 10.74
Molecular weight: 7.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.899
IPC2_protein 7.849
IPC_protein 8.2
Toseland 7.936
ProMoST 8.741
Dawson 8.492
Bjellqvist 8.741
Wikipedia 8.463
Rodwell 8.478
Grimsley 7.658
Solomon 8.873
Lehninger 8.873
Nozaki 8.858
DTASelect 8.536
Thurlkill 8.565
EMBOSS 8.726
Sillero 8.873
Patrickios 0.896
IPC_peptide 8.858
IPC2_peptide 8.156
IPC2.peptide.svr19 8.036
Protein with the highest isoelectric point:
>tr|M1XJC1|M1XJC1_9VIRU Truncated beta C1 protein OS=Cotton leaf curl Multan betasatellite OX=306025 GN=C1 PE=4 SV=1
MM1 pKa = 7.17 VKK3 pKa = 9.96 PRR5 pKa = 11.84 SHH7 pKa = 6.72 LQTTTFSDD15 pKa = 3.39 MFLFCLFFYY24 pKa = 10.72 YY25 pKa = 10.28 SQTMTFSNLYY35 pKa = 9.48 FFLFTFYY42 pKa = 10.61 FFSFLGFSCFDD53 pKa = 3.38 SLRR56 pKa = 11.84 SHH58 pKa = 7.51 FFHH61 pKa = 7.66 KK62 pKa = 10.74
Molecular weight: 7.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.899
IPC2_protein 7.849
IPC_protein 8.2
Toseland 7.936
ProMoST 8.741
Dawson 8.492
Bjellqvist 8.741
Wikipedia 8.463
Rodwell 8.478
Grimsley 7.658
Solomon 8.873
Lehninger 8.873
Nozaki 8.858
DTASelect 8.536
Thurlkill 8.565
EMBOSS 8.726
Sillero 8.873
Patrickios 0.896
IPC_peptide 8.858
IPC2_peptide 8.156
IPC2.peptide.svr19 8.036
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
62
62
62
62.0
7.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
0.0 ± 0.0
3.226 ± 0.0
3.226 ± 0.0
0.0 ± 0.0
27.419 ± 0.0
1.613 ± 0.0
4.839 ± 0.0
0.0 ± 0.0
3.226 ± 0.0
11.29 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.839 ± 0.0
1.613 ± 0.0
1.613 ± 0.0
3.226 ± 0.0
3.226 ± 0.0
12.903 ± 0.0
9.677 ± 0.0
1.613 ± 0.0
0.0 ± 0.0
6.452 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here