Lagenorhynchus acutus papillomavirus
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2VIS6|F2VIS6_9PAPI Regulatory protein E2 OS=Lagenorhynchus acutus papillomavirus OX=706530 GN=E2 PE=3 SV=1
MM1 pKa = 7.49 DD2 pKa = 5.06 TSPGTDD8 pKa = 3.51 PLEE11 pKa = 4.7 GGCNDD16 pKa = 3.24 WVLLEE21 pKa = 4.11 AAEE24 pKa = 4.54 AGGSGIDD31 pKa = 3.57 EE32 pKa = 4.39 EE33 pKa = 4.44 EE34 pKa = 5.66 GEE36 pKa = 4.82 DD37 pKa = 4.03 EE38 pKa = 4.94 DD39 pKa = 6.69 DD40 pKa = 4.54 SGEE43 pKa = 4.39 DD44 pKa = 3.24 MLDD47 pKa = 4.33 FIDD50 pKa = 6.17 DD51 pKa = 3.45 SSTFQNAEE59 pKa = 3.78 TQLYY63 pKa = 9.34 CRR65 pKa = 11.84 QLQVEE70 pKa = 4.24 QQRR73 pKa = 11.84 IDD75 pKa = 3.71 DD76 pKa = 3.98 EE77 pKa = 4.87 RR78 pKa = 11.84 VAQALKK84 pKa = 10.61 RR85 pKa = 11.84 KK86 pKa = 9.29 FLDD89 pKa = 3.61 SPKK92 pKa = 10.75 SKK94 pKa = 10.57 VDD96 pKa = 3.54 SEE98 pKa = 4.24 LSPRR102 pKa = 11.84 LAAISLQEE110 pKa = 3.72 QQRR113 pKa = 11.84 RR114 pKa = 11.84 GRR116 pKa = 11.84 ARR118 pKa = 11.84 RR119 pKa = 11.84 RR120 pKa = 11.84 LYY122 pKa = 10.58 KK123 pKa = 10.64 DD124 pKa = 2.99 SGHH127 pKa = 6.54 GDD129 pKa = 3.36 SLEE132 pKa = 4.38 AEE134 pKa = 4.57 SEE136 pKa = 3.99 EE137 pKa = 5.23 SMVCRR142 pKa = 11.84 NVQVPKK148 pKa = 10.11 NQTMDD153 pKa = 3.21 AVGAGEE159 pKa = 4.02 GARR162 pKa = 11.84 PKK164 pKa = 10.57 EE165 pKa = 4.12 VGNASTQEE173 pKa = 4.08 VQCAGMSEE181 pKa = 4.42 EE182 pKa = 4.53 SACSVTQILRR192 pKa = 11.84 AGKK195 pKa = 9.04 PRR197 pKa = 11.84 ALLLGVFKK205 pKa = 10.63 EE206 pKa = 4.3 AFGCSFADD214 pKa = 3.49 LTRR217 pKa = 11.84 CFKK220 pKa = 10.71 SDD222 pKa = 3.09 KK223 pKa = 9.94 TVNDD227 pKa = 3.65 DD228 pKa = 3.31 WTCFIVGVPCSLEE241 pKa = 3.97 GAISDD246 pKa = 4.06 LLKK249 pKa = 10.58 PSTLFTHH256 pKa = 5.85 VTTATSKK263 pKa = 10.97 YY264 pKa = 9.42 GLTVLLLVQWKK275 pKa = 7.96 TGKK278 pKa = 10.03 SRR280 pKa = 11.84 EE281 pKa = 4.38 TVGNTLAGLLSVDD294 pKa = 3.48 KK295 pKa = 10.75 QQIVCEE301 pKa = 4.12 PPKK304 pKa = 10.15 IRR306 pKa = 11.84 HH307 pKa = 6.0 LGAAMFWYY315 pKa = 10.17 KK316 pKa = 10.57 KK317 pKa = 9.88 AISSACVVTGEE328 pKa = 4.13 MPVWILKK335 pKa = 9.21 QVSIQDD341 pKa = 3.51 QLGDD345 pKa = 3.43 VCQFSLSVMIQWAYY359 pKa = 11.29 DD360 pKa = 3.46 NGHH363 pKa = 6.33 DD364 pKa = 3.83 TEE366 pKa = 4.17 EE367 pKa = 5.15 AIAYY371 pKa = 7.49 EE372 pKa = 4.3 YY373 pKa = 11.18 AALADD378 pKa = 3.76 EE379 pKa = 5.25 DD380 pKa = 4.38 KK381 pKa = 11.21 NAEE384 pKa = 3.76 AFLRR388 pKa = 11.84 SNCQPKK394 pKa = 9.82 YY395 pKa = 11.15 VKK397 pKa = 10.49 DD398 pKa = 3.74 CANMLRR404 pKa = 11.84 LYY406 pKa = 10.52 KK407 pKa = 9.99 RR408 pKa = 11.84 AEE410 pKa = 3.82 MRR412 pKa = 11.84 KK413 pKa = 8.42 MSMGQWIKK421 pKa = 10.58 HH422 pKa = 5.65 KK423 pKa = 10.28 CDD425 pKa = 3.22 AVQGEE430 pKa = 4.77 GDD432 pKa = 3.62 WKK434 pKa = 10.9 HH435 pKa = 5.91 VMNFLKK441 pKa = 10.72 FQGIEE446 pKa = 3.78 IMPFLIDD453 pKa = 4.22 FRR455 pKa = 11.84 LFLKK459 pKa = 10.08 GIPKK463 pKa = 9.5 RR464 pKa = 11.84 NCMVLYY470 pKa = 10.16 GPPNTGKK477 pKa = 10.5 SLFAMSLIHH486 pKa = 7.02 LLGGKK491 pKa = 8.88 VLSYY495 pKa = 11.26 VNSSSHH501 pKa = 6.44 FWLQPVADD509 pKa = 4.63 CKK511 pKa = 11.08 VALIDD516 pKa = 5.26 DD517 pKa = 4.58 ATASTWDD524 pKa = 3.48 YY525 pKa = 11.51 ADD527 pKa = 3.31 TYY529 pKa = 10.59 LRR531 pKa = 11.84 NVLDD535 pKa = 4.54 GNPMSLDD542 pKa = 3.9 SKK544 pKa = 10.75 HH545 pKa = 6.22 KK546 pKa = 10.68 APMQITCPPLLITTNCNVLEE566 pKa = 4.17 NNKK569 pKa = 8.69 WKK571 pKa = 10.35 YY572 pKa = 8.63 LHH574 pKa = 6.59 SRR576 pKa = 11.84 LRR578 pKa = 11.84 VYY580 pKa = 10.62 NFHH583 pKa = 6.91 NEE585 pKa = 3.89 CPLNSRR591 pKa = 11.84 GEE593 pKa = 4.14 PEE595 pKa = 3.97 FQLTKK600 pKa = 10.74 VNWKK604 pKa = 10.57 AFFNKK609 pKa = 9.44 CWAKK613 pKa = 10.79 LSLDD617 pKa = 4.02 EE618 pKa = 5.34 GDD620 pKa = 4.06 EE621 pKa = 4.2 GHH623 pKa = 6.59 NGSPLQPLRR632 pKa = 11.84 CVARR636 pKa = 11.84 AADD639 pKa = 3.71 EE640 pKa = 4.44 ADD642 pKa = 3.13
Molecular weight: 71.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.191
IPC2_protein 5.258
IPC_protein 5.232
Toseland 5.232
ProMoST 5.359
Dawson 5.27
Bjellqvist 5.397
Wikipedia 5.169
Rodwell 5.194
Grimsley 5.181
Solomon 5.27
Lehninger 5.245
Nozaki 5.423
DTASelect 5.588
Thurlkill 5.296
EMBOSS 5.258
Sillero 5.499
Patrickios 4.037
IPC_peptide 5.283
IPC2_peptide 5.486
IPC2.peptide.svr19 5.495
Protein with the highest isoelectric point:
>tr|F2VIS7|F2VIS7_9PAPI E4 protein (Fragment) OS=Lagenorhynchus acutus papillomavirus OX=706530 GN=E4 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 7.83 EE3 pKa = 4.08 TKK5 pKa = 9.57 DD6 pKa = 3.47 TMEE9 pKa = 4.24 VLCSRR14 pKa = 11.84 LDD16 pKa = 3.6 ALQEE20 pKa = 3.93 QQMKK24 pKa = 10.69 LIEE27 pKa = 4.43 TDD29 pKa = 4.55 DD30 pKa = 4.08 GDD32 pKa = 4.23 LEE34 pKa = 4.57 NVLLYY39 pKa = 10.63 FGLLRR44 pKa = 11.84 KK45 pKa = 8.29 EE46 pKa = 4.27 TMLLYY51 pKa = 10.38 AANEE55 pKa = 4.08 RR56 pKa = 11.84 GHH58 pKa = 6.39 KK59 pKa = 10.06 KK60 pKa = 10.45 VGFTMVPNKK69 pKa = 9.76 KK70 pKa = 10.08 ACEE73 pKa = 3.86 EE74 pKa = 3.94 NAKK77 pKa = 10.3 KK78 pKa = 10.23 CIRR81 pKa = 11.84 MHH83 pKa = 6.51 LAVSSLLSSLFAKK96 pKa = 9.26 EE97 pKa = 4.1 KK98 pKa = 9.92 WFLSQVSYY106 pKa = 11.49 EE107 pKa = 4.28 MFQTPPVEE115 pKa = 4.28 TFKK118 pKa = 11.36 KK119 pKa = 9.52 EE120 pKa = 3.69 GRR122 pKa = 11.84 TVTVTFDD129 pKa = 4.45 DD130 pKa = 4.83 DD131 pKa = 3.92 EE132 pKa = 4.8 QNSMEE137 pKa = 4.38 YY138 pKa = 10.56 VCWTRR143 pKa = 11.84 IYY145 pKa = 10.37 CQLPNGEE152 pKa = 4.27 WGCYY156 pKa = 8.43 CAVVCYY162 pKa = 10.23 EE163 pKa = 4.27 GIYY166 pKa = 10.42 YY167 pKa = 10.35 DD168 pKa = 3.69 VEE170 pKa = 4.15 GEE172 pKa = 3.86 KK173 pKa = 10.45 RR174 pKa = 11.84 FYY176 pKa = 11.51 VDD178 pKa = 5.11 FLSEE182 pKa = 3.88 ALKK185 pKa = 11.11 YY186 pKa = 9.33 GTGEE190 pKa = 3.83 KK191 pKa = 8.11 WTVLYY196 pKa = 10.43 DD197 pKa = 3.72 GKK199 pKa = 10.9 AITDD203 pKa = 3.8 CTLVSSTSTTSPTSALGYY221 pKa = 9.08 PILSSTPGNPGGEE234 pKa = 4.05 PAHH237 pKa = 5.67 TKK239 pKa = 9.94 RR240 pKa = 11.84 RR241 pKa = 11.84 RR242 pKa = 11.84 KK243 pKa = 8.56 PQTRR247 pKa = 11.84 RR248 pKa = 11.84 LLGPPPSPPPSPPPGRR264 pKa = 11.84 PPPPRR269 pKa = 11.84 PPTPPPRR276 pKa = 11.84 PPATPPSPLATPGSAKK292 pKa = 10.02 RR293 pKa = 11.84 HH294 pKa = 5.92 RR295 pKa = 11.84 EE296 pKa = 3.46 TSAAAGAEE304 pKa = 4.1 GRR306 pKa = 11.84 GGSGDD311 pKa = 3.51 SFGGVSEE318 pKa = 4.62 CDD320 pKa = 3.54 CNRR323 pKa = 11.84 DD324 pKa = 3.05 SGKK327 pKa = 9.99 RR328 pKa = 11.84 RR329 pKa = 11.84 RR330 pKa = 11.84 TDD332 pKa = 3.3 PLIPCLLIGGGANQVKK348 pKa = 10.01 CLRR351 pKa = 11.84 HH352 pKa = 5.31 RR353 pKa = 11.84 LKK355 pKa = 10.94 VQFFGLYY362 pKa = 9.91 SNCSTTWSWVGHH374 pKa = 5.92 SGSTPNHH381 pKa = 6.64 RR382 pKa = 11.84 ICVSFLNEE390 pKa = 3.55 EE391 pKa = 3.86 QRR393 pKa = 11.84 NIFLNFVPLPGTVTVAPAEE412 pKa = 4.24 LPFF415 pKa = 5.72
Molecular weight: 45.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.233
IPC2_protein 7.541
IPC_protein 7.337
Toseland 6.883
ProMoST 7.936
Dawson 8.053
Bjellqvist 8.536
Wikipedia 7.951
Rodwell 8.068
Grimsley 6.898
Solomon 8.097
Lehninger 8.126
Nozaki 8.799
DTASelect 8.2
Thurlkill 8.244
EMBOSS 8.273
Sillero 8.609
Patrickios 4.177
IPC_peptide 8.097
IPC2_peptide 7.629
IPC2.peptide.svr19 7.603
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2460
137
642
410.0
45.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.626 ± 0.513
2.48 ± 0.695
5.935 ± 0.535
5.813 ± 0.626
3.78 ± 0.334
6.951 ± 0.384
2.846 ± 1.027
3.699 ± 0.452
4.512 ± 0.921
9.431 ± 0.449
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.789 ± 0.41
3.821 ± 0.502
7.073 ± 1.058
3.577 ± 0.675
6.179 ± 0.536
8.618 ± 0.914
5.894 ± 0.573
6.504 ± 0.377
1.504 ± 0.229
2.967 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here