Hymenobacter sp. CRA2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter; unclassified Hymenobacter

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S9ART8|A0A1S9ART8_9BACT Ferredoxin--NADP reductase OS=Hymenobacter sp. CRA2 OX=1955620 GN=B0919_22255 PE=3 SV=1
MM1 pKa = 7.78ISTKK5 pKa = 10.58NYY7 pKa = 10.06AALPDD12 pKa = 3.83AAALQQLCKK21 pKa = 10.92AMAVLDD27 pKa = 5.07AINSPEE33 pKa = 3.53WEE35 pKa = 4.27YY36 pKa = 10.93RR37 pKa = 11.84YY38 pKa = 10.3HH39 pKa = 8.16SYY41 pKa = 11.38DD42 pKa = 3.63PAWGLDD48 pKa = 3.3EE49 pKa = 6.68AVFQLNTGEE58 pKa = 4.13GDD60 pKa = 3.27EE61 pKa = 4.19MLVIFRR67 pKa = 11.84PEE69 pKa = 4.09GCCINGFIDD78 pKa = 6.49GYY80 pKa = 10.43AQPDD84 pKa = 3.74KK85 pKa = 11.42AQVTQGLPAAFEE97 pKa = 4.05EE98 pKa = 5.17FVFGEE103 pKa = 4.49PVASIGTTFCLWYY116 pKa = 9.39TPTQGWQTGVVDD128 pKa = 4.78EE129 pKa = 4.79NADD132 pKa = 3.9DD133 pKa = 4.63GSEE136 pKa = 3.96EE137 pKa = 3.95MLYY140 pKa = 10.89LFDD143 pKa = 5.08GRR145 pKa = 11.84PEE147 pKa = 5.0AYY149 pKa = 9.28TEE151 pKa = 3.82WANEE155 pKa = 3.88YY156 pKa = 10.13FVEE159 pKa = 4.27DD160 pKa = 3.64TGRR163 pKa = 11.84QLIDD167 pKa = 3.31AADD170 pKa = 3.48VSRR173 pKa = 11.84VYY175 pKa = 10.54RR176 pKa = 11.84GEE178 pKa = 4.03VLTKK182 pKa = 10.41EE183 pKa = 3.76AVQRR187 pKa = 11.84INEE190 pKa = 4.15EE191 pKa = 4.19TEE193 pKa = 3.79DD194 pKa = 3.7WAQLEE199 pKa = 4.4ADD201 pKa = 4.21LQEE204 pKa = 4.37IGYY207 pKa = 8.89PYY209 pKa = 10.82DD210 pKa = 3.44FSKK213 pKa = 11.39

Molecular weight: 23.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S9AQY9|A0A1S9AQY9_9BACT DDE_Tnp_1 domain-containing protein OS=Hymenobacter sp. CRA2 OX=1955620 GN=B0919_23455 PE=4 SV=1
MM1 pKa = 7.05PHH3 pKa = 4.79VVKK6 pKa = 10.64YY7 pKa = 10.91ASVFLLSMVKK17 pKa = 10.42FFGGPLAGAAAGLPFMAIWALTVAGMMTSVVVFTGVGRR55 pKa = 11.84AFTAHH60 pKa = 6.17LQRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84LRR68 pKa = 11.84HH69 pKa = 4.5QPIFTRR75 pKa = 11.84RR76 pKa = 11.84SRR78 pKa = 11.84QIVRR82 pKa = 11.84VFSRR86 pKa = 11.84FGIGGIAFLTPILFTPIGGAVIATLLGVKK115 pKa = 9.61RR116 pKa = 11.84QHH118 pKa = 6.75ILLHH122 pKa = 5.55MLWSALLWGAAGAFLAIRR140 pKa = 11.84FSHH143 pKa = 6.57LFIRR147 pKa = 11.84HH148 pKa = 4.9

Molecular weight: 16.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4792

0

4792

1648900

26

2720

344.1

37.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.886 ± 0.049

0.729 ± 0.01

4.962 ± 0.024

5.221 ± 0.042

3.873 ± 0.023

7.748 ± 0.042

2.118 ± 0.022

4.002 ± 0.031

3.544 ± 0.037

10.983 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.816 ± 0.017

3.606 ± 0.038

5.324 ± 0.029

5.02 ± 0.027

6.504 ± 0.036

5.345 ± 0.038

6.168 ± 0.055

7.244 ± 0.029

1.361 ± 0.015

3.548 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski