Lactobacillus rhamnosus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2657 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249DG04|A0A249DG04_LACRH AAA_23 domain-containing protein OS=Lactobacillus rhamnosus OX=47715 GN=BGK71_11955 PE=4 SV=1
MM1 pKa = 6.39MTSTLTVVGRR11 pKa = 11.84EE12 pKa = 4.07VFIDD16 pKa = 4.21DD17 pKa = 3.89YY18 pKa = 11.83NEE20 pKa = 4.79EE21 pKa = 3.78IDD23 pKa = 3.53NDD25 pKa = 3.71YY26 pKa = 11.22RR27 pKa = 11.84LDD29 pKa = 4.06PDD31 pKa = 5.36EE32 pKa = 4.97ILQDD36 pKa = 3.38MMEE39 pKa = 4.46LMEE42 pKa = 5.25EE43 pKa = 4.28SPEE46 pKa = 4.35SYY48 pKa = 10.16QHH50 pKa = 6.28LHH52 pKa = 6.66FDD54 pKa = 4.54SEE56 pKa = 4.42QTNDD60 pKa = 3.29GTNKK64 pKa = 9.94LFSFTSYY71 pKa = 10.41EE72 pKa = 4.21GEE74 pKa = 4.39DD75 pKa = 3.91GLRR78 pKa = 11.84LSYY81 pKa = 10.93LGVSDD86 pKa = 4.08EE87 pKa = 4.24

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3D3U4|A0A0E3D3U4_LACRH Membrane protein OS=Lactobacillus rhamnosus OX=47715 GN=BGK71_07215 PE=4 SV=1
MM1 pKa = 5.72TTKK4 pKa = 9.85RR5 pKa = 11.84TFQPKK10 pKa = 8.33KK11 pKa = 7.5RR12 pKa = 11.84HH13 pKa = 5.56RR14 pKa = 11.84EE15 pKa = 3.79RR16 pKa = 11.84VHH18 pKa = 7.7GFMKK22 pKa = 10.56RR23 pKa = 11.84MSTKK27 pKa = 10.26NGRR30 pKa = 11.84KK31 pKa = 8.58VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.94GRR41 pKa = 11.84KK42 pKa = 8.67VLSAA46 pKa = 4.05

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2657

0

2657

823911

37

3575

310.1

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.738 ± 0.079

0.383 ± 0.01

5.753 ± 0.053

4.682 ± 0.054

4.287 ± 0.04

6.884 ± 0.038

2.337 ± 0.024

6.481 ± 0.043

5.616 ± 0.049

10.265 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.028

4.061 ± 0.03

4.104 ± 0.031

4.784 ± 0.042

4.396 ± 0.039

5.482 ± 0.054

6.509 ± 0.059

7.168 ± 0.035

1.118 ± 0.019

3.271 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski