Trichoplax sp. H2
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12150 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369RR91|A0A369RR91_9METZ Uncharacterized protein (Fragment) OS=Trichoplax sp. H2 OX=287889 GN=TrispH2_010911 PE=4 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.95 SDD4 pKa = 3.52 SKK6 pKa = 10.65 LTSEE10 pKa = 4.51 EE11 pKa = 4.37 SEE13 pKa = 3.76 ISLPSSIIDD22 pKa = 4.25 DD23 pKa = 4.54 GCCVAPFYY31 pKa = 9.88 TQNDD35 pKa = 4.18 SLNDD39 pKa = 4.49 DD40 pKa = 3.5 ATCWKK45 pKa = 10.74 NEE47 pKa = 3.82 EE48 pKa = 4.29 NDD50 pKa = 4.13 NINNDD55 pKa = 3.3 HH56 pKa = 6.74 SPTYY60 pKa = 8.69 ITCPTGLNDD69 pKa = 3.58 QDD71 pKa = 4.28 SINRR75 pKa = 11.84 NLTTHH80 pKa = 6.09 HH81 pKa = 6.96 TDD83 pKa = 3.34 DD84 pKa = 6.0 DD85 pKa = 4.68 GDD87 pKa = 3.98 NLHH90 pKa = 7.49 DD91 pKa = 4.35 RR92 pKa = 11.84 LTNLMQKK99 pKa = 10.59 LDD101 pKa = 3.84 TTQSSVAVKK110 pKa = 9.44 TVDD113 pKa = 3.69 KK114 pKa = 11.13 LLTTAINRR122 pKa = 11.84 YY123 pKa = 9.72 SDD125 pKa = 3.21 DD126 pKa = 3.36 QYY128 pKa = 11.31 RR129 pKa = 11.84 CRR131 pKa = 11.84 AFQIARR137 pKa = 11.84 CILQQYY143 pKa = 9.34 GMEE146 pKa = 4.58 PFQSLQTDD154 pKa = 3.65 SLKK157 pKa = 11.09 YY158 pKa = 10.09 PGLTQDD164 pKa = 3.43 VLDD167 pKa = 4.4 YY168 pKa = 11.22 LNEE171 pKa = 4.25 VQLSTMDD178 pKa = 4.61 EE179 pKa = 4.29 IATSS183 pKa = 3.42
Molecular weight: 20.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.795
IPC2_protein 4.012
IPC_protein 4.012
Toseland 3.795
ProMoST 4.19
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.961
Rodwell 3.846
Grimsley 3.706
Solomon 4.012
Lehninger 3.961
Nozaki 4.126
DTASelect 4.406
Thurlkill 3.846
EMBOSS 3.973
Sillero 4.139
Patrickios 1.952
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.016
Protein with the highest isoelectric point:
>tr|A0A369RZF1|A0A369RZF1_9METZ Uncharacterized protein PYUK71.03c OS=Trichoplax sp. H2 OX=287889 GN=TrispH2_007539 PE=4 SV=1
MM1 pKa = 7.28 ATPRR5 pKa = 11.84 LCRR8 pKa = 11.84 TNLNLICCFQKK19 pKa = 10.72 CLNNIHH25 pKa = 6.68 APSGLQRR32 pKa = 11.84 AGIATTPIILGTKK45 pKa = 9.76 AKK47 pKa = 10.23 RR48 pKa = 11.84 PPRR51 pKa = 11.84 GVNLDD56 pKa = 3.2 KK57 pKa = 10.86 RR58 pKa = 11.84 IRR60 pKa = 11.84 PYY62 pKa = 10.51 RR63 pKa = 11.84 YY64 pKa = 8.01 GVTVASEE71 pKa = 4.21 SQNTGCISGSRR82 pKa = 11.84 QWTRR86 pKa = 11.84 EE87 pKa = 3.89 RR88 pKa = 11.84 ITDD91 pKa = 3.57 DD92 pKa = 3.26 LMIRR96 pKa = 11.84 SFIRR100 pKa = 11.84 GTFLPFLDD108 pKa = 4.47 HH109 pKa = 7.14 KK110 pKa = 10.59 RR111 pKa = 11.84 QTVVIKK117 pKa = 10.63 RR118 pKa = 11.84 KK119 pKa = 10.04 LNTIIVALFVVPRR132 pKa = 11.84 DD133 pKa = 3.67 QISRR137 pKa = 11.84 IYY139 pKa = 10.5 FLVGYY144 pKa = 9.71 AEE146 pKa = 4.4 KK147 pKa = 11.05 LMGAMFGCNVQLVLQTGHH165 pKa = 5.99 YY166 pKa = 9.89
Molecular weight: 18.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.721
IPC_protein 10.511
Toseland 10.628
ProMoST 10.35
Dawson 10.745
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.965
Grimsley 10.789
Solomon 10.833
Lehninger 10.804
Nozaki 10.643
DTASelect 10.452
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.672
Patrickios 10.672
IPC_peptide 10.847
IPC2_peptide 9.736
IPC2.peptide.svr19 8.459
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12150
0
12150
6586016
20
8009
542.1
61.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.921 ± 0.023
2.079 ± 0.027
5.647 ± 0.022
5.78 ± 0.034
3.98 ± 0.016
4.868 ± 0.025
2.341 ± 0.011
7.328 ± 0.023
6.636 ± 0.026
9.321 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.155 ± 0.01
6.052 ± 0.022
3.887 ± 0.019
4.321 ± 0.017
4.895 ± 0.017
8.619 ± 0.028
5.746 ± 0.021
5.588 ± 0.015
1.067 ± 0.008
3.767 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here