Pseudactinotalea sp. HY158

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Pseudactinotalea; unclassified Pseudactinotalea

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3052 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2VZ47|A0A5Q2VZ47_9CELL Cytochrome bc1 complex Rieske iron-sulfur subunit OS=Pseudactinotalea sp. HY158 OX=2654547 GN=GCE65_06835 PE=4 SV=1
MM1 pKa = 7.55AATVSGCLALLTGPIAAFATTGGAGEE27 pKa = 4.44AGGNAAEE34 pKa = 4.49RR35 pKa = 11.84EE36 pKa = 4.21PARR39 pKa = 11.84LTIVSDD45 pKa = 3.2ATGAIGAGEE54 pKa = 4.53APSFSYY60 pKa = 11.13GSTSDD65 pKa = 3.42VAGSSWGEE73 pKa = 4.1GFTLAPTPEE82 pKa = 5.05DD83 pKa = 3.75PDD85 pKa = 3.87EE86 pKa = 4.31LTGALLPGEE95 pKa = 4.39AVTVEE100 pKa = 4.09AEE102 pKa = 4.28LPQGWVLDD110 pKa = 4.37DD111 pKa = 3.92LTCTGGDD118 pKa = 3.73DD119 pKa = 3.79VTVGATSVTVTSASDD134 pKa = 3.55VSCTFTNAPVATTGEE149 pKa = 4.36ADD151 pKa = 3.71PADD154 pKa = 4.4PADD157 pKa = 4.25PADD160 pKa = 4.25PADD163 pKa = 4.05PADD166 pKa = 4.39PDD168 pKa = 3.72VAEE171 pKa = 5.15PEE173 pKa = 4.36PADD176 pKa = 3.77EE177 pKa = 5.16PEE179 pKa = 4.6PADD182 pKa = 4.06APASAARR189 pKa = 11.84PSSADD194 pKa = 3.06VHH196 pKa = 6.26RR197 pKa = 11.84AGIHH201 pKa = 5.51PADD204 pKa = 4.24MQPMAVGEE212 pKa = 4.32APVCSSGYY220 pKa = 10.08VYY222 pKa = 10.71SVYY225 pKa = 10.88DD226 pKa = 3.73GGDD229 pKa = 3.25LVQIDD234 pKa = 3.9PSGAVSTMGSWSSFVDD250 pKa = 4.41GPGGSVNGLAIGDD263 pKa = 4.09GGTVVYY269 pKa = 11.1AFMRR273 pKa = 11.84FSSDD277 pKa = 2.97TAEE280 pKa = 4.85DD281 pKa = 3.89PLSNATVLRR290 pKa = 11.84YY291 pKa = 9.76LPATDD296 pKa = 3.15AWEE299 pKa = 4.22SLGTLNSNTTQQLIAGAVDD318 pKa = 4.89FASGDD323 pKa = 3.9YY324 pKa = 10.95FFGGYY329 pKa = 10.08DD330 pKa = 3.2SSNNFQLWRR339 pKa = 11.84YY340 pKa = 9.69DD341 pKa = 3.28VGTGLFDD348 pKa = 3.26KK349 pKa = 10.95VGYY352 pKa = 9.25FVSGLVGANGDD363 pKa = 3.79MAFDD367 pKa = 3.28AHH369 pKa = 6.72GNLFVIASDD378 pKa = 3.68DD379 pKa = 3.98DD380 pKa = 5.06GNVTEE385 pKa = 5.56FMITAATLAAAEE397 pKa = 4.21NNALDD402 pKa = 4.84LSQTHH407 pKa = 6.31AFDD410 pKa = 3.55VGGVAVNGVAFDD422 pKa = 4.34ADD424 pKa = 3.39GSIYY428 pKa = 10.41LGSDD432 pKa = 3.12TTVYY436 pKa = 10.53KK437 pKa = 10.64YY438 pKa = 11.01DD439 pKa = 3.47PTTWTSLGTHH449 pKa = 6.6TGNLVDD455 pKa = 3.96STDD458 pKa = 3.8LASCNSPGTITVRR471 pKa = 11.84KK472 pKa = 10.01DD473 pKa = 2.79ITLRR477 pKa = 11.84AASADD482 pKa = 3.57QFRR485 pKa = 11.84LTLEE489 pKa = 4.07RR490 pKa = 11.84DD491 pKa = 3.44AVTVATEE498 pKa = 4.26DD499 pKa = 3.42TSGTTTGLQPTQIGPVPAISGADD522 pKa = 3.44YY523 pKa = 10.93SFSEE527 pKa = 5.12AIIAFPGSSQMSAYY541 pKa = 9.15TSTWEE546 pKa = 4.21CEE548 pKa = 3.89AGGSVIATGTGTAGVVTIPPPLSGDD573 pKa = 3.21EE574 pKa = 4.23GAHH577 pKa = 4.86VVCTFTNAPKK587 pKa = 9.9TNPIEE592 pKa = 4.26FTKK595 pKa = 10.27RR596 pKa = 11.84WQGAIAGDD604 pKa = 3.77TAEE607 pKa = 4.81LSMSGFALVDD617 pKa = 3.3ATTATSTADD626 pKa = 3.39GTNPQHH632 pKa = 6.68DD633 pKa = 4.46TVNVATGAAAYY644 pKa = 9.41GSSVTLAEE652 pKa = 4.6NIGHH656 pKa = 6.72GSDD659 pKa = 3.79PAQGSYY665 pKa = 10.23NATYY669 pKa = 9.08TCTQGGVTVAVAAQTFVMPFTDD691 pKa = 3.87GTVEE695 pKa = 4.15CVVTNTPRR703 pKa = 11.84TGNIVVTKK711 pKa = 9.63KK712 pKa = 9.7WVVQDD717 pKa = 3.31SAGNVVEE724 pKa = 5.06TYY726 pKa = 10.25DD727 pKa = 3.62IPADD731 pKa = 3.78ASTLPAGLHH740 pKa = 5.84AQLTLDD746 pKa = 3.83GQNRR750 pKa = 11.84AWDD753 pKa = 3.9ASDD756 pKa = 3.02SRR758 pKa = 11.84TIGSVVTIDD767 pKa = 4.14EE768 pKa = 4.37VATLDD773 pKa = 3.69GDD775 pKa = 4.31ALPGCSILEE784 pKa = 4.09PPVLTFEE791 pKa = 4.24GTIATFAPTPGEE803 pKa = 4.34DD804 pKa = 3.0YY805 pKa = 11.17GLPFDD810 pKa = 4.08ATVAAGTSRR819 pKa = 11.84FHH821 pKa = 5.8ITNTIEE827 pKa = 4.31CTQSLSLVKK836 pKa = 10.38QVDD839 pKa = 3.86VGAVTADD846 pKa = 3.02TWTLSATKK854 pKa = 9.64PDD856 pKa = 3.15GSAALEE862 pKa = 4.79GPSGTYY868 pKa = 10.46SPTTPVTAAVTATVPYY884 pKa = 10.46ALAEE888 pKa = 4.39AGGPATYY895 pKa = 10.52VPDD898 pKa = 4.45GGWVCATSDD907 pKa = 3.82SEE909 pKa = 4.5AVAVTDD915 pKa = 3.97GAVEE919 pKa = 4.31VPLGADD925 pKa = 3.51VTCTITNTTALLTVLKK941 pKa = 10.17HH942 pKa = 6.3IDD944 pKa = 3.56GSSDD948 pKa = 3.65LSPSDD953 pKa = 3.31WDD955 pKa = 3.65LTATPDD961 pKa = 3.38AGVAGLTPTTVPGADD976 pKa = 3.78GAATDD981 pKa = 4.58PNAASTFEE989 pKa = 3.77VRR991 pKa = 11.84PGHH994 pKa = 6.21GYY996 pKa = 10.52SLAEE1000 pKa = 3.95ALAAGHH1006 pKa = 6.93EE1007 pKa = 4.28DD1008 pKa = 3.31TAYY1011 pKa = 10.87LQLALQHH1018 pKa = 6.49WDD1020 pKa = 2.95GAEE1023 pKa = 4.01WVDD1026 pKa = 3.49VDD1028 pKa = 3.99ADD1030 pKa = 4.24TVSVEE1035 pKa = 4.08AGAHH1039 pKa = 4.8DD1040 pKa = 4.08VYY1042 pKa = 11.17RR1043 pKa = 11.84FVNAGVTAISLPLTGGLGTDD1063 pKa = 4.14LFLALGALGLIAAVALAGWQHH1084 pKa = 6.27RR1085 pKa = 11.84RR1086 pKa = 11.84TTRR1089 pKa = 11.84MRR1091 pKa = 11.84RR1092 pKa = 11.84AA1093 pKa = 3.22

Molecular weight: 110.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W5T7|A0A5Q2W5T7_9CELL Oligoribonuclease OS=Pseudactinotalea sp. HY158 OX=2654547 GN=GCE65_11990 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.69GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.77GRR40 pKa = 11.84ASVSAA45 pKa = 3.98

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3052

0

3052

1062837

32

2300

348.2

37.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.99 ± 0.07

0.587 ± 0.012

6.347 ± 0.047

5.683 ± 0.037

2.735 ± 0.026

9.628 ± 0.047

2.15 ± 0.023

4.024 ± 0.03

1.44 ± 0.024

10.007 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.753 ± 0.018

1.763 ± 0.022

5.881 ± 0.039

2.559 ± 0.026

7.808 ± 0.06

5.399 ± 0.029

6.31 ± 0.046

8.532 ± 0.043

1.464 ± 0.018

1.938 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski