Terriglobus saanensis (strain ATCC BAA-1853 / DSM 23119 / SP1PR4)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4177 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E8V6D7|E8V6D7_TERSS Uncharacterized protein OS=Terriglobus saanensis (strain ATCC BAA-1853 / DSM 23119 / SP1PR4) OX=401053 GN=AciPR4_0769 PE=4 SV=1
MM1 pKa = 7.23 KK2 pKa = 10.33 RR3 pKa = 11.84 SLICAGTILVGLTLLSGCGNSSNNGIGSTTPATPPTPLVAGGAANIVVVQNNTLNVATASDD64 pKa = 4.62 SILKK68 pKa = 10.53 FSTATNGIVAPATSITGPAGFFLSGVATDD97 pKa = 3.55 STGNVYY103 pKa = 8.25 VTAVSTTTSVSEE115 pKa = 3.85 ILVYY119 pKa = 10.43 DD120 pKa = 4.43 ANATGAATPMRR131 pKa = 11.84 TITSTSLIFPIGITVDD147 pKa = 3.53 SAGSIYY153 pKa = 10.96 VSDD156 pKa = 4.37 APSNSLSNLSNPSSIFVFDD175 pKa = 4.35 PKK177 pKa = 11.33 ANGVSIPTRR186 pKa = 11.84 TITGAATLLNQPLSLAVDD204 pKa = 3.88 TAGNLYY210 pKa = 10.74 VSDD213 pKa = 4.68 LVQLGISVFSPTATGNVAPASTISGPATGLVFPANGVALDD253 pKa = 3.93 ASNNIYY259 pKa = 10.39 VSSADD264 pKa = 3.3 PTGNFGVVAEE274 pKa = 5.02 FAAGATGNVAPIKK287 pKa = 9.24 TVGGATLGANQGLGGVEE304 pKa = 3.7 VDD306 pKa = 3.73 SVGNIYY312 pKa = 9.15 TVGVFGAFNSFFEE325 pKa = 4.5 LAAFAPTATGDD336 pKa = 3.51 VTPAILDD343 pKa = 3.54 TASAFNISNFGLLAIHH359 pKa = 7.33
Molecular weight: 35.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.719
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.923
Rodwell 3.77
Grimsley 3.63
Solomon 3.935
Lehninger 3.897
Nozaki 4.075
DTASelect 4.355
Thurlkill 3.795
EMBOSS 3.923
Sillero 4.075
Patrickios 1.952
IPC_peptide 3.935
IPC2_peptide 4.037
IPC2.peptide.svr19 3.953
Protein with the highest isoelectric point:
>tr|E8V745|E8V745_TERSS Uncharacterized protein OS=Terriglobus saanensis (strain ATCC BAA-1853 / DSM 23119 / SP1PR4) OX=401053 GN=AciPR4_0852 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.02 RR4 pKa = 11.84 TFQPNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.87 THH17 pKa = 5.78 GFLTRR22 pKa = 11.84 MKK24 pKa = 9.16 TKK26 pKa = 10.54 AGQAVLNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.03 GRR40 pKa = 11.84 HH41 pKa = 5.72 KK42 pKa = 10.29 IAVSAGFRR50 pKa = 11.84 DD51 pKa = 3.66
Molecular weight: 5.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4177
0
4177
1519388
30
2699
363.8
39.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.644 ± 0.038
0.814 ± 0.011
4.989 ± 0.025
5.363 ± 0.038
4.068 ± 0.025
7.944 ± 0.038
2.275 ± 0.017
5.066 ± 0.028
3.857 ± 0.028
9.992 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.301 ± 0.018
3.479 ± 0.037
5.177 ± 0.026
3.794 ± 0.023
5.981 ± 0.038
6.549 ± 0.035
6.383 ± 0.05
7.245 ± 0.034
1.334 ± 0.015
2.745 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here