Listeria booriae
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3104 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A099W950|A0A099W950_9LIST Uncharacterized protein OS=Listeria booriae OX=1552123 GN=EP57_06610 PE=4 SV=1
MM1 pKa = 7.29 TVLLLIFFGFLLFLCLVALIVGVILFIVRR30 pKa = 11.84 KK31 pKa = 9.85 GKK33 pKa = 8.79 MLGALMMAGSLVLGFPLFGGFMASIIMAAPMEE65 pKa = 4.14 YY66 pKa = 10.29 HH67 pKa = 6.48 NNSTMMHH74 pKa = 7.57 DD75 pKa = 4.43 DD76 pKa = 3.99 LMDD79 pKa = 4.18 GSTYY83 pKa = 11.13 DD84 pKa = 3.65 DD85 pKa = 3.7 TGGSDD90 pKa = 4.3 DD91 pKa = 3.72 SSSYY95 pKa = 11.38 YY96 pKa = 10.51 DD97 pKa = 5.35 DD98 pKa = 3.94 SDD100 pKa = 4.54 EE101 pKa = 4.63 YY102 pKa = 10.98 TDD104 pKa = 5.65 DD105 pKa = 3.56 WDD107 pKa = 3.57 EE108 pKa = 4.67 DD109 pKa = 3.96 EE110 pKa = 5.54 YY111 pKa = 12.02 YY112 pKa = 10.96 SDD114 pKa = 6.23 DD115 pKa = 3.44 YY116 pKa = 10.82 TQYY119 pKa = 11.53 KK120 pKa = 9.13 VGQAAEE126 pKa = 4.29 FQDD129 pKa = 4.34 GTWVTVSKK137 pKa = 10.82 LEE139 pKa = 3.9 KK140 pKa = 8.49 WTGDD144 pKa = 3.71 SEE146 pKa = 4.67 FQTPMAGFYY155 pKa = 10.83 VKK157 pKa = 10.1 ATILLEE163 pKa = 4.37 NKK165 pKa = 10.08 GKK167 pKa = 8.78 EE168 pKa = 4.35 TVDD171 pKa = 4.48 FYY173 pKa = 11.84 ADD175 pKa = 3.2 EE176 pKa = 4.26 FQIYY180 pKa = 10.2 DD181 pKa = 3.33 QDD183 pKa = 3.93 NFDD186 pKa = 4.48 GEE188 pKa = 4.46 NTYY191 pKa = 10.97 KK192 pKa = 10.69 EE193 pKa = 4.18 DD194 pKa = 3.37 WNLSLKK200 pKa = 10.45 PGAKK204 pKa = 8.82 ATRR207 pKa = 11.84 TVYY210 pKa = 10.7 FDD212 pKa = 3.43 VYY214 pKa = 11.13 GNGPFHH220 pKa = 6.93 VVLSDD225 pKa = 3.49 VSWSGSVKK233 pKa = 10.61
Molecular weight: 26.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.719
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.872
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.304
Thurlkill 3.77
EMBOSS 3.884
Sillero 4.062
Patrickios 1.24
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.93
Protein with the highest isoelectric point:
>tr|A0A099WB28|A0A099WB28_9LIST ATP-dependent protease subunit HslV OS=Listeria booriae OX=1552123 GN=hslV PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSKK9 pKa = 9.59 RR10 pKa = 11.84 KK11 pKa = 9.09 RR12 pKa = 11.84 KK13 pKa = 8.75 KK14 pKa = 9.02 VHH16 pKa = 5.87 GFRR19 pKa = 11.84 QRR21 pKa = 11.84 MSSKK25 pKa = 9.81 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3104
0
3104
990240
37
2366
319.0
35.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.229 ± 0.057
0.626 ± 0.011
5.576 ± 0.051
6.65 ± 0.046
4.548 ± 0.04
6.764 ± 0.034
1.847 ± 0.022
7.469 ± 0.048
6.489 ± 0.04
9.421 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.748 ± 0.025
4.433 ± 0.035
3.531 ± 0.027
3.834 ± 0.031
3.863 ± 0.03
5.62 ± 0.031
6.43 ± 0.07
7.347 ± 0.044
1.011 ± 0.018
3.565 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here