Roseomonas mucosa

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S8D997|A0A1S8D997_9PROT Putative DNA modification/repair radical SAM protein OS=Roseomonas mucosa OX=207340 GN=APZ41_005880 PE=4 SV=1
MM1 pKa = 7.43ATTTNSRR8 pKa = 11.84PIAVADD14 pKa = 3.98RR15 pKa = 11.84VAVSEE20 pKa = 4.72DD21 pKa = 3.13GVLQATGNLLSNDD34 pKa = 3.12TDD36 pKa = 3.89ADD38 pKa = 4.43RR39 pKa = 11.84DD40 pKa = 4.16TLAVTAVNGLAAGVGTVIAGTYY62 pKa = 9.93GSLVLGANGQYY73 pKa = 10.5TYY75 pKa = 11.72VLANAQANVQALAAGQTVTEE95 pKa = 4.39SFTYY99 pKa = 9.58TVSDD103 pKa = 4.06GQAHH107 pKa = 6.92ADD109 pKa = 3.22AGEE112 pKa = 3.86NLIAQSEE119 pKa = 4.55AFGDD123 pKa = 4.09ASWVPFNGNGALPTVAADD141 pKa = 3.43AATGPTGTAAADD153 pKa = 3.95VLSLPADD160 pKa = 3.89GGLYY164 pKa = 8.77TAAAVSGPHH173 pKa = 5.8TFSVWVRR180 pKa = 11.84LVSGDD185 pKa = 3.37GNFAFNYY192 pKa = 10.12YY193 pKa = 10.49DD194 pKa = 3.56GTSDD198 pKa = 4.51SIQSGTATGEE208 pKa = 3.79WQRR211 pKa = 11.84FSWTFTGNGNGDD223 pKa = 3.74ANVAILHH230 pKa = 6.67DD231 pKa = 4.61LASSTGSVLEE241 pKa = 3.74IWGAQLNSGGSAGAYY256 pKa = 8.82VATTGTPVTGTTVTTQNLIAQSEE279 pKa = 4.41AFDD282 pKa = 4.29DD283 pKa = 4.54ASWGHH288 pKa = 5.11FTVTGTQPTVTANAATGPTGSGTADD313 pKa = 3.53LLGLSGIGTGIYY325 pKa = 10.7ANTAVSGQYY334 pKa = 9.05TFSVWVRR341 pKa = 11.84LASGDD346 pKa = 3.72GNFSFNYY353 pKa = 9.67FDD355 pKa = 4.25GSSNHH360 pKa = 5.82LQAATATGAWQRR372 pKa = 11.84FSWTFDD378 pKa = 3.27ANGDD382 pKa = 3.68GNANVALMHH391 pKa = 6.99DD392 pKa = 4.78LATATSGVLEE402 pKa = 4.15LWGAQLNQGAAADD415 pKa = 4.27SYY417 pKa = 11.01IATAGSPVTVTSAGTGSEE435 pKa = 4.27TATLTVAIAGAADD448 pKa = 3.4IGTGGPALPVAVADD462 pKa = 4.46RR463 pKa = 11.84AAVSEE468 pKa = 4.51DD469 pKa = 3.09GVLQATGNLLSNDD482 pKa = 3.12TDD484 pKa = 3.89ADD486 pKa = 4.43RR487 pKa = 11.84DD488 pKa = 4.16TLAVTAVNGLAAGVGTVIAGTYY510 pKa = 9.93GSLVLGANGQYY521 pKa = 10.5TYY523 pKa = 11.27ILANAQANVQALEE536 pKa = 4.28AGRR539 pKa = 11.84SVTDD543 pKa = 2.87VFRR546 pKa = 11.84YY547 pKa = 7.37TVSDD551 pKa = 4.11GASHH555 pKa = 8.02TITTQNLLPYY565 pKa = 10.1SEE567 pKa = 5.77ALDD570 pKa = 3.57NPAWVRR576 pKa = 11.84FSNNGASATVMADD589 pKa = 3.12AALSPIGSRR598 pKa = 11.84TAEE601 pKa = 4.33LLSMTGSGTGIYY613 pKa = 9.72TGTDD617 pKa = 2.96VSGQSTFSAWIRR629 pKa = 11.84LAAGDD634 pKa = 3.61GHH636 pKa = 7.0FSFNYY641 pKa = 9.43FDD643 pKa = 4.17GGSNHH648 pKa = 5.72FQSALATAEE657 pKa = 4.12WQHH660 pKa = 5.32FTWTFTGSGSNSANVAILHH679 pKa = 6.46DD680 pKa = 4.87LSTFTSGSFEE690 pKa = 3.44IWGAQLNAGATADD703 pKa = 3.88TYY705 pKa = 11.28LATSGTPATQASTGVASFGSTLTVTITGASDD736 pKa = 3.35TPAAAATLSLADD748 pKa = 3.71HH749 pKa = 7.27ADD751 pKa = 3.34GVVANLATGQWAQALKK767 pKa = 10.53IMPLGDD773 pKa = 4.03SITYY777 pKa = 8.96GWRR780 pKa = 11.84QIDD783 pKa = 3.58TNYY786 pKa = 10.99GYY788 pKa = 10.95LQDD791 pKa = 4.42NLANGYY797 pKa = 7.75RR798 pKa = 11.84QPLWAEE804 pKa = 3.8FTDD807 pKa = 4.23RR808 pKa = 11.84DD809 pKa = 3.63MLINYY814 pKa = 8.58VGTQSSGTGSLPDD827 pKa = 3.99HH828 pKa = 7.08DD829 pKa = 4.74HH830 pKa = 7.31AGFPGWKK837 pKa = 9.54AEE839 pKa = 4.03EE840 pKa = 4.38LATILPGLLGGGAPDD855 pKa = 4.58AVLLMAGANDD865 pKa = 3.79VFRR868 pKa = 11.84EE869 pKa = 4.03DD870 pKa = 3.87AAQNTAGTDD879 pKa = 3.09IKK881 pKa = 11.71AMLNAIAAANPATHH895 pKa = 6.57VYY897 pKa = 10.54VSTILPTAWADD908 pKa = 3.37AAEE911 pKa = 4.41VNAVNAVIRR920 pKa = 11.84STVQSAIAGGQHH932 pKa = 4.11VTLVEE937 pKa = 4.23MPGVTTADD945 pKa = 3.83MYY947 pKa = 11.55DD948 pKa = 4.22GIHH951 pKa = 5.96PTEE954 pKa = 4.19AGYY957 pKa = 11.38AEE959 pKa = 4.34MAQYY963 pKa = 9.24WLNAILAAQGNGGGTPGGSAQAIASGLTSVLGGGGNDD1000 pKa = 4.16LLIGDD1005 pKa = 4.44TRR1007 pKa = 11.84ANTLNGADD1015 pKa = 3.82GNDD1018 pKa = 3.29RR1019 pKa = 11.84LTGGGAADD1027 pKa = 3.96TLIGGAGADD1036 pKa = 3.1EE1037 pKa = 4.71FVFRR1041 pKa = 11.84PVAGNVVVQDD1051 pKa = 4.01FSTAEE1056 pKa = 3.94KK1057 pKa = 10.74DD1058 pKa = 3.59VLVFDD1063 pKa = 5.86GIAGLSNFAALSGHH1077 pKa = 5.51VAQANGATVIDD1088 pKa = 4.25LSGFGTPVQITLQGFAGGLAADD1110 pKa = 4.2NVWFSS1115 pKa = 3.43

Molecular weight:
112.44 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S8D7R9|A0A1S8D7R9_9PROT Methyltransferase OS=Roseomonas mucosa OX=207340 GN=APZ41_008790 PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 8.42GTPMSLWLSAASRR15 pKa = 11.84TAGWWQGHH23 pKa = 5.75FANAWRR29 pKa = 11.84QQSSKK34 pKa = 10.78AIRR37 pKa = 11.84AALSAPAKK45 pKa = 9.73PSRR48 pKa = 11.84TTRR51 pKa = 11.84TRR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 4.64TKK57 pKa = 8.39KK58 pKa = 9.05TT59 pKa = 3.39

Molecular weight:
6.62 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4204

0

4204

1351983

22

2515

321.6

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.957 ± 0.061

0.84 ± 0.011

4.987 ± 0.025

5.829 ± 0.038

3.287 ± 0.025

9.469 ± 0.032

2.051 ± 0.018

3.958 ± 0.025

2.078 ± 0.029

11.357 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.016

2.028 ± 0.02

6.265 ± 0.034

2.984 ± 0.022

8.5 ± 0.039

4.689 ± 0.025

4.754 ± 0.024

7.266 ± 0.029

1.487 ± 0.017

1.814 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski