Roseomonas mucosa
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4204 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S8D997|A0A1S8D997_9PROT Putative DNA modification/repair radical SAM protein OS=Roseomonas mucosa OX=207340 GN=APZ41_005880 PE=4 SV=1
MM1 pKa = 7.43 ATTTNSRR8 pKa = 11.84 PIAVADD14 pKa = 3.98 RR15 pKa = 11.84 VAVSEE20 pKa = 4.72 DD21 pKa = 3.13 GVLQATGNLLSNDD34 pKa = 3.12 TDD36 pKa = 3.89 ADD38 pKa = 4.43 RR39 pKa = 11.84 DD40 pKa = 4.16 TLAVTAVNGLAAGVGTVIAGTYY62 pKa = 9.93 GSLVLGANGQYY73 pKa = 10.5 TYY75 pKa = 11.72 VLANAQANVQALAAGQTVTEE95 pKa = 4.39 SFTYY99 pKa = 9.58 TVSDD103 pKa = 4.06 GQAHH107 pKa = 6.92 ADD109 pKa = 3.22 AGEE112 pKa = 3.86 NLIAQSEE119 pKa = 4.55 AFGDD123 pKa = 4.09 ASWVPFNGNGALPTVAADD141 pKa = 3.43 AATGPTGTAAADD153 pKa = 3.95 VLSLPADD160 pKa = 3.89 GGLYY164 pKa = 8.77 TAAAVSGPHH173 pKa = 5.8 TFSVWVRR180 pKa = 11.84 LVSGDD185 pKa = 3.37 GNFAFNYY192 pKa = 10.12 YY193 pKa = 10.49 DD194 pKa = 3.56 GTSDD198 pKa = 4.51 SIQSGTATGEE208 pKa = 3.79 WQRR211 pKa = 11.84 FSWTFTGNGNGDD223 pKa = 3.74 ANVAILHH230 pKa = 6.67 DD231 pKa = 4.61 LASSTGSVLEE241 pKa = 3.74 IWGAQLNSGGSAGAYY256 pKa = 8.82 VATTGTPVTGTTVTTQNLIAQSEE279 pKa = 4.41 AFDD282 pKa = 4.29 DD283 pKa = 4.54 ASWGHH288 pKa = 5.11 FTVTGTQPTVTANAATGPTGSGTADD313 pKa = 3.53 LLGLSGIGTGIYY325 pKa = 10.7 ANTAVSGQYY334 pKa = 9.05 TFSVWVRR341 pKa = 11.84 LASGDD346 pKa = 3.72 GNFSFNYY353 pKa = 9.67 FDD355 pKa = 4.25 GSSNHH360 pKa = 5.82 LQAATATGAWQRR372 pKa = 11.84 FSWTFDD378 pKa = 3.27 ANGDD382 pKa = 3.68 GNANVALMHH391 pKa = 6.99 DD392 pKa = 4.78 LATATSGVLEE402 pKa = 4.15 LWGAQLNQGAAADD415 pKa = 4.27 SYY417 pKa = 11.01 IATAGSPVTVTSAGTGSEE435 pKa = 4.27 TATLTVAIAGAADD448 pKa = 3.4 IGTGGPALPVAVADD462 pKa = 4.46 RR463 pKa = 11.84 AAVSEE468 pKa = 4.51 DD469 pKa = 3.09 GVLQATGNLLSNDD482 pKa = 3.12 TDD484 pKa = 3.89 ADD486 pKa = 4.43 RR487 pKa = 11.84 DD488 pKa = 4.16 TLAVTAVNGLAAGVGTVIAGTYY510 pKa = 9.93 GSLVLGANGQYY521 pKa = 10.5 TYY523 pKa = 11.27 ILANAQANVQALEE536 pKa = 4.28 AGRR539 pKa = 11.84 SVTDD543 pKa = 2.87 VFRR546 pKa = 11.84 YY547 pKa = 7.37 TVSDD551 pKa = 4.11 GASHH555 pKa = 8.02 TITTQNLLPYY565 pKa = 10.1 SEE567 pKa = 5.77 ALDD570 pKa = 3.57 NPAWVRR576 pKa = 11.84 FSNNGASATVMADD589 pKa = 3.12 AALSPIGSRR598 pKa = 11.84 TAEE601 pKa = 4.33 LLSMTGSGTGIYY613 pKa = 9.72 TGTDD617 pKa = 2.96 VSGQSTFSAWIRR629 pKa = 11.84 LAAGDD634 pKa = 3.61 GHH636 pKa = 7.0 FSFNYY641 pKa = 9.43 FDD643 pKa = 4.17 GGSNHH648 pKa = 5.72 FQSALATAEE657 pKa = 4.12 WQHH660 pKa = 5.32 FTWTFTGSGSNSANVAILHH679 pKa = 6.46 DD680 pKa = 4.87 LSTFTSGSFEE690 pKa = 3.44 IWGAQLNAGATADD703 pKa = 3.88 TYY705 pKa = 11.28 LATSGTPATQASTGVASFGSTLTVTITGASDD736 pKa = 3.35 TPAAAATLSLADD748 pKa = 3.71 HH749 pKa = 7.27 ADD751 pKa = 3.34 GVVANLATGQWAQALKK767 pKa = 10.53 IMPLGDD773 pKa = 4.03 SITYY777 pKa = 8.96 GWRR780 pKa = 11.84 QIDD783 pKa = 3.58 TNYY786 pKa = 10.99 GYY788 pKa = 10.95 LQDD791 pKa = 4.42 NLANGYY797 pKa = 7.75 RR798 pKa = 11.84 QPLWAEE804 pKa = 3.8 FTDD807 pKa = 4.23 RR808 pKa = 11.84 DD809 pKa = 3.63 MLINYY814 pKa = 8.58 VGTQSSGTGSLPDD827 pKa = 3.99 HH828 pKa = 7.08 DD829 pKa = 4.74 HH830 pKa = 7.31 AGFPGWKK837 pKa = 9.54 AEE839 pKa = 4.03 EE840 pKa = 4.38 LATILPGLLGGGAPDD855 pKa = 4.58 AVLLMAGANDD865 pKa = 3.79 VFRR868 pKa = 11.84 EE869 pKa = 4.03 DD870 pKa = 3.87 AAQNTAGTDD879 pKa = 3.09 IKK881 pKa = 11.71 AMLNAIAAANPATHH895 pKa = 6.57 VYY897 pKa = 10.54 VSTILPTAWADD908 pKa = 3.37 AAEE911 pKa = 4.41 VNAVNAVIRR920 pKa = 11.84 STVQSAIAGGQHH932 pKa = 4.11 VTLVEE937 pKa = 4.23 MPGVTTADD945 pKa = 3.83 MYY947 pKa = 11.55 DD948 pKa = 4.22 GIHH951 pKa = 5.96 PTEE954 pKa = 4.19 AGYY957 pKa = 11.38 AEE959 pKa = 4.34 MAQYY963 pKa = 9.24 WLNAILAAQGNGGGTPGGSAQAIASGLTSVLGGGGNDD1000 pKa = 4.16 LLIGDD1005 pKa = 4.44 TRR1007 pKa = 11.84 ANTLNGADD1015 pKa = 3.82 GNDD1018 pKa = 3.29 RR1019 pKa = 11.84 LTGGGAADD1027 pKa = 3.96 TLIGGAGADD1036 pKa = 3.1 EE1037 pKa = 4.71 FVFRR1041 pKa = 11.84 PVAGNVVVQDD1051 pKa = 4.01 FSTAEE1056 pKa = 3.94 KK1057 pKa = 10.74 DD1058 pKa = 3.59 VLVFDD1063 pKa = 5.86 GIAGLSNFAALSGHH1077 pKa = 5.51 VAQANGATVIDD1088 pKa = 4.25 LSGFGTPVQITLQGFAGGLAADD1110 pKa = 4.2 NVWFSS1115 pKa = 3.43
Molecular weight: 112.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.884
IPC_protein 3.935
Toseland 3.706
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.757
Grimsley 3.605
Solomon 3.935
Lehninger 3.897
Nozaki 4.037
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.897
Sillero 4.062
Patrickios 1.329
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A1S8D7R9|A0A1S8D7R9_9PROT Methyltransferase OS=Roseomonas mucosa OX=207340 GN=APZ41_008790 PE=3 SV=1
MM1 pKa = 7.65 KK2 pKa = 8.42 GTPMSLWLSAASRR15 pKa = 11.84 TAGWWQGHH23 pKa = 5.75 FANAWRR29 pKa = 11.84 QQSSKK34 pKa = 10.78 AIRR37 pKa = 11.84 AALSAPAKK45 pKa = 9.73 PSRR48 pKa = 11.84 TTRR51 pKa = 11.84 TRR53 pKa = 11.84 RR54 pKa = 11.84 HH55 pKa = 4.64 TKK57 pKa = 8.39 KK58 pKa = 9.05 TT59 pKa = 3.39
Molecular weight: 6.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.544
Grimsley 12.837
Solomon 13.276
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.281
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.072
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4204
0
4204
1351983
22
2515
321.6
34.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.957 ± 0.061
0.84 ± 0.011
4.987 ± 0.025
5.829 ± 0.038
3.287 ± 0.025
9.469 ± 0.032
2.051 ± 0.018
3.958 ± 0.025
2.078 ± 0.029
11.357 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.4 ± 0.016
2.028 ± 0.02
6.265 ± 0.034
2.984 ± 0.022
8.5 ± 0.039
4.689 ± 0.025
4.754 ± 0.024
7.266 ± 0.029
1.487 ± 0.017
1.814 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here