Intestinimonas butyriciproducens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Intestinimonas

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3358 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2W2M6|A0A0S2W2M6_9FIRM Uncharacterized protein OS=Intestinimonas butyriciproducens OX=1297617 GN=IB211_01126c PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.67NKK4 pKa = 9.59IKK6 pKa = 10.68KK7 pKa = 9.93SFAAILALAMLLSLAACGGKK27 pKa = 10.14DD28 pKa = 3.62KK29 pKa = 11.42GSDD32 pKa = 3.38GDD34 pKa = 4.46GSDD37 pKa = 3.29KK38 pKa = 11.29TEE40 pKa = 4.16TIVLGKK46 pKa = 8.19YY47 pKa = 6.62TAVFKK52 pKa = 10.87GYY54 pKa = 8.09TLTKK58 pKa = 10.45DD59 pKa = 4.32DD60 pKa = 4.69EE61 pKa = 5.2GNDD64 pKa = 3.69AMVLTYY70 pKa = 10.51DD71 pKa = 3.64YY72 pKa = 11.25TNGSKK77 pKa = 10.69DD78 pKa = 3.55EE79 pKa = 4.25QSFAWAFLFEE89 pKa = 4.12ATQAGEE95 pKa = 4.14EE96 pKa = 4.62LGSPMYY102 pKa = 10.54DD103 pKa = 3.11EE104 pKa = 5.57NNNQITKK111 pKa = 10.44NYY113 pKa = 8.24EE114 pKa = 3.83EE115 pKa = 4.6KK116 pKa = 10.68VKK118 pKa = 10.53KK119 pKa = 10.52GQTLEE124 pKa = 3.89VAIALTLVNTTDD136 pKa = 3.32DD137 pKa = 4.15VIIVFSDD144 pKa = 3.77FDD146 pKa = 3.73DD147 pKa = 4.43HH148 pKa = 7.67EE149 pKa = 4.4YY150 pKa = 9.09TQTVKK155 pKa = 10.96LSGSGVQDD163 pKa = 3.34SGPEE167 pKa = 3.87EE168 pKa = 4.03AGSYY172 pKa = 8.63QLYY175 pKa = 10.6EE176 pKa = 3.9MTFEE180 pKa = 5.11GDD182 pKa = 3.69VFDD185 pKa = 5.78HH186 pKa = 7.46DD187 pKa = 4.23FLEE190 pKa = 4.65TSGFEE195 pKa = 4.36LYY197 pKa = 10.56TMEE200 pKa = 5.03LAADD204 pKa = 3.82GTGWLQIDD212 pKa = 3.98GRR214 pKa = 11.84GSLTWGDD221 pKa = 3.63GKK223 pKa = 9.47ITIDD227 pKa = 4.14GSDD230 pKa = 3.11AVYY233 pKa = 9.44TYY235 pKa = 9.56TYY237 pKa = 9.28KK238 pKa = 11.07DD239 pKa = 3.22GMIVLEE245 pKa = 4.25EE246 pKa = 4.21STGEE250 pKa = 3.98SMTFQKK256 pKa = 9.77TGAAGTSGGLKK267 pKa = 10.07PVGGDD272 pKa = 3.29GSDD275 pKa = 3.92ANLTAYY281 pKa = 8.18QQYY284 pKa = 10.37WNGDD288 pKa = 3.06WYY290 pKa = 11.17GWTAYY295 pKa = 11.21SNGTNDD301 pKa = 3.27YY302 pKa = 10.86SSLDD306 pKa = 3.86GFYY309 pKa = 8.99MDD311 pKa = 4.01CMARR315 pKa = 11.84IEE317 pKa = 4.01IDD319 pKa = 3.6EE320 pKa = 4.49NGEE323 pKa = 3.79GALILWDD330 pKa = 3.75EE331 pKa = 4.5ATSVDD336 pKa = 4.11DD337 pKa = 3.85PRR339 pKa = 11.84AGVTVCISDD348 pKa = 3.44GGFEE352 pKa = 3.95QGRR355 pKa = 11.84LKK357 pKa = 11.05SEE359 pKa = 4.83DD360 pKa = 2.96GWFSDD365 pKa = 4.16GDD367 pKa = 3.73VGNADD372 pKa = 3.62WLVDD376 pKa = 3.89PDD378 pKa = 3.79GLGYY382 pKa = 10.93EE383 pKa = 4.06NLICIEE389 pKa = 4.34GSYY392 pKa = 10.66KK393 pKa = 11.08DD394 pKa = 3.82PDD396 pKa = 4.19DD397 pKa = 4.34FFSGYY402 pKa = 10.79DD403 pKa = 3.22YY404 pKa = 10.96TIYY407 pKa = 10.54LRR409 pKa = 11.84PWGLDD414 pKa = 2.91WADD417 pKa = 3.82IEE419 pKa = 5.27ADD421 pKa = 3.83QPDD424 pKa = 4.96GLPDD428 pKa = 3.7HH429 pKa = 6.83YY430 pKa = 11.22DD431 pKa = 3.04WYY433 pKa = 11.25LSAIQTGAKK442 pKa = 8.52MPDD445 pKa = 3.92VIGGDD450 pKa = 3.84YY451 pKa = 11.07GGLAPGEE458 pKa = 4.41VPDD461 pKa = 5.16SQMEE465 pKa = 4.17PQGGNDD471 pKa = 3.7PNASAVDD478 pKa = 4.18GTWQAPVYY486 pKa = 10.67GDD488 pKa = 3.62YY489 pKa = 11.36GLSKK493 pKa = 10.91ASATGVVPMTRR504 pKa = 11.84DD505 pKa = 3.38EE506 pKa = 4.18LVDD509 pKa = 4.54RR510 pKa = 11.84IGFYY514 pKa = 10.97ADD516 pKa = 3.5SQIGSAPYY524 pKa = 10.07DD525 pKa = 3.28SLYY528 pKa = 11.29NSMGYY533 pKa = 8.93IHH535 pKa = 7.53GKK537 pKa = 9.02PLIDD541 pKa = 4.58DD542 pKa = 4.39PRR544 pKa = 11.84WDD546 pKa = 3.27TGKK549 pKa = 9.33IHH551 pKa = 6.72VYY553 pKa = 8.5QWSIEE558 pKa = 4.17SGEE561 pKa = 4.11YY562 pKa = 8.66ATLVFEE568 pKa = 4.41VTDD571 pKa = 3.53YY572 pKa = 11.54SIGIEE577 pKa = 3.94KK578 pKa = 9.78LQSVEE583 pKa = 4.17TSPGLLDD590 pKa = 3.61GG591 pKa = 4.84

Molecular weight: 64.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2W113|A0A0S2W113_9FIRM Uncharacterized protein OS=Intestinimonas butyriciproducens OX=1297617 GN=IB211_00606c PE=4 SV=1
MM1 pKa = 7.88VGILAAFSRR10 pKa = 11.84LRR12 pKa = 11.84LPWARR17 pKa = 11.84VSRR20 pKa = 11.84LRR22 pKa = 11.84RR23 pKa = 11.84GQFSQNIQKK32 pKa = 9.88FQGFVLL38 pKa = 3.98

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3358

0

3358

986929

37

3097

293.9

32.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.003 ± 0.06

1.651 ± 0.019

5.504 ± 0.041

6.767 ± 0.048

3.779 ± 0.029

8.316 ± 0.045

1.793 ± 0.019

5.269 ± 0.041

4.352 ± 0.042

10.184 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.025

3.008 ± 0.029

4.388 ± 0.031

3.157 ± 0.027

6.096 ± 0.047

5.561 ± 0.043

5.565 ± 0.049

7.325 ± 0.034

1.085 ± 0.016

3.439 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski