Wuhan Millipede virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLL2|A0A1L3KLL2_9VIRU Uncharacterized protein OS=Wuhan Millipede virus 4 OX=1923689 PE=4 SV=1
MM1 pKa = 7.19EE2 pKa = 5.91RR3 pKa = 11.84IVRR6 pKa = 11.84KK7 pKa = 8.86PVFRR11 pKa = 11.84HH12 pKa = 5.01YY13 pKa = 11.14DD14 pKa = 3.23SYY16 pKa = 11.4EE17 pKa = 3.96QPTTPEE23 pKa = 4.31DD24 pKa = 3.43PSEE27 pKa = 3.95RR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.78RR31 pKa = 11.84RR32 pKa = 11.84DD33 pKa = 3.19ALRR36 pKa = 11.84HH37 pKa = 5.25RR38 pKa = 11.84RR39 pKa = 11.84FTSMLEE45 pKa = 3.8PLPARR50 pKa = 11.84EE51 pKa = 4.35PYY53 pKa = 10.26AMNYY57 pKa = 9.53KK58 pKa = 9.3PAKK61 pKa = 8.93IDD63 pKa = 3.67RR64 pKa = 11.84NGLFAMNTVFPPAAVSAIRR83 pKa = 11.84TKK85 pKa = 8.22YY86 pKa = 9.76HH87 pKa = 5.97RR88 pKa = 11.84AALNFEE94 pKa = 4.47DD95 pKa = 5.66LKK97 pKa = 11.57ADD99 pKa = 3.9LLEE102 pKa = 4.39YY103 pKa = 11.06GCDD106 pKa = 3.24KK107 pKa = 10.62PSRR110 pKa = 11.84NVSPNYY116 pKa = 9.62YY117 pKa = 10.38AILEE121 pKa = 4.31SVRR124 pKa = 11.84RR125 pKa = 11.84DD126 pKa = 3.01LALPKK131 pKa = 10.62NSIIPLTTGAVTNNPDD147 pKa = 3.45FPNSKK152 pKa = 10.59SPGFPYY158 pKa = 10.11KK159 pKa = 10.74AQGIRR164 pKa = 11.84TKK166 pKa = 10.59RR167 pKa = 11.84EE168 pKa = 3.78AVDD171 pKa = 3.53TPGVLDD177 pKa = 4.73EE178 pKa = 5.37IRR180 pKa = 11.84RR181 pKa = 11.84IWYY184 pKa = 9.11DD185 pKa = 3.17VEE187 pKa = 4.1AGRR190 pKa = 11.84EE191 pKa = 4.04VEE193 pKa = 5.01LPDD196 pKa = 3.26VACYY200 pKa = 10.07HH201 pKa = 7.1RR202 pKa = 11.84AQICTRR208 pKa = 11.84DD209 pKa = 3.52KK210 pKa = 11.46NKK212 pKa = 9.45IRR214 pKa = 11.84ATWGYY219 pKa = 9.05PLAVYY224 pKa = 9.75LAEE227 pKa = 4.28GAYY230 pKa = 9.69FYY232 pKa = 10.73PVLDD236 pKa = 3.65HH237 pKa = 6.98LKK239 pKa = 9.86NLSSSPIAYY248 pKa = 9.15GVEE251 pKa = 3.98MANGGMQYY259 pKa = 11.03VNAAVQHH266 pKa = 6.03FPNQPMLVGDD276 pKa = 3.78WSKK279 pKa = 11.12FDD281 pKa = 3.4KK282 pKa = 10.77TIPAWLIRR290 pKa = 11.84DD291 pKa = 3.47AFKK294 pKa = 10.49IIEE297 pKa = 4.06EE298 pKa = 5.04GIDD301 pKa = 3.6FSHH304 pKa = 6.7VQDD307 pKa = 3.77SDD309 pKa = 3.67GKK311 pKa = 8.17IWPVRR316 pKa = 11.84EE317 pKa = 3.61EE318 pKa = 3.97RR319 pKa = 11.84SRR321 pKa = 11.84KK322 pKa = 7.94RR323 pKa = 11.84WRR325 pKa = 11.84KK326 pKa = 8.42LVSYY330 pKa = 10.14FIDD333 pKa = 3.61TPIRR337 pKa = 11.84LSDD340 pKa = 3.36GSRR343 pKa = 11.84YY344 pKa = 8.97QKK346 pKa = 10.71HH347 pKa = 6.15SGVPSGACFTNIIDD361 pKa = 4.63SIVNMIVMRR370 pKa = 11.84YY371 pKa = 9.47CIYY374 pKa = 10.62EE375 pKa = 3.84FTGQLPLFDD384 pKa = 5.0MYY386 pKa = 11.27LGDD389 pKa = 4.8DD390 pKa = 4.37SIIVLPEE397 pKa = 4.78LIDD400 pKa = 3.82LGEE403 pKa = 3.82FSKK406 pKa = 11.03YY407 pKa = 10.6AKK409 pKa = 9.56EE410 pKa = 3.83QFGMEE415 pKa = 4.18FSDD418 pKa = 3.35TKK420 pKa = 11.27SRR422 pKa = 11.84ITYY425 pKa = 10.08RR426 pKa = 11.84PDD428 pKa = 3.0FVHH431 pKa = 7.06FLGYY435 pKa = 10.51YY436 pKa = 8.11NQNGTPAKK444 pKa = 10.7SLDD447 pKa = 3.45TTIASMIYY455 pKa = 9.65PEE457 pKa = 4.01HH458 pKa = 5.69TVRR461 pKa = 11.84DD462 pKa = 3.72KK463 pKa = 11.81LEE465 pKa = 4.41TITRR469 pKa = 11.84CVGQAYY475 pKa = 10.4SCFEE479 pKa = 4.78PYY481 pKa = 10.45DD482 pKa = 3.35ATAFFLAAKK491 pKa = 9.76VLAEE495 pKa = 4.11EE496 pKa = 4.34EE497 pKa = 4.25HH498 pKa = 6.4LSRR501 pKa = 11.84EE502 pKa = 4.27VVEE505 pKa = 4.53SVIRR509 pKa = 11.84THH511 pKa = 6.71PMRR514 pKa = 11.84FKK516 pKa = 10.58YY517 pKa = 10.63LMTIGVDD524 pKa = 3.33PEE526 pKa = 4.83SIVFPDD532 pKa = 3.5VQPNDD537 pKa = 3.86LCLLTQPGQMRR548 pKa = 11.84RR549 pKa = 11.84AYY551 pKa = 10.32RR552 pKa = 11.84FRR554 pKa = 11.84AYY556 pKa = 10.3DD557 pKa = 3.31FHH559 pKa = 8.61DD560 pKa = 5.08LYY562 pKa = 10.96TAGVSHH568 pKa = 7.6FYY570 pKa = 11.43YY571 pKa = 10.64EE572 pKa = 5.52DD573 pKa = 4.81DD574 pKa = 4.6DD575 pKa = 4.28SS576 pKa = 5.11

Molecular weight: 66.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLC9|A0A1L3KLC9_9VIRU Uncharacterized protein OS=Wuhan Millipede virus 4 OX=1923689 PE=4 SV=1
MM1 pKa = 7.77ADD3 pKa = 4.19APNEE7 pKa = 4.06APKK10 pKa = 10.7QPGVYY15 pKa = 9.5HH16 pKa = 6.83GKK18 pKa = 9.62PEE20 pKa = 4.23NKK22 pKa = 9.6KK23 pKa = 10.4NPHH26 pKa = 6.38ALQGPHH32 pKa = 6.72RR33 pKa = 11.84GKK35 pKa = 9.78PAPHH39 pKa = 6.42AQKK42 pKa = 10.89APLPSGPPPPSQGNSKK58 pKa = 10.25KK59 pKa = 9.73PAKK62 pKa = 10.4GDD64 pKa = 3.69FKK66 pKa = 11.16EE67 pKa = 4.41SDD69 pKa = 3.32PHH71 pKa = 7.95ADD73 pKa = 3.86AIFSTSVCARR83 pKa = 11.84EE84 pKa = 3.54IAIDD88 pKa = 3.68VRR90 pKa = 11.84QQPSCSSFPEE100 pKa = 4.33LVSLVYY106 pKa = 10.43QEE108 pKa = 4.68LAIDD112 pKa = 3.79ATSLPKK118 pKa = 10.06QWTRR122 pKa = 11.84EE123 pKa = 3.85AFEE126 pKa = 4.71YY127 pKa = 11.01YY128 pKa = 9.12CTAHH132 pKa = 5.57LWMRR136 pKa = 11.84IVHH139 pKa = 6.58LKK141 pKa = 10.42AKK143 pKa = 10.11QRR145 pKa = 11.84DD146 pKa = 3.79HH147 pKa = 5.88LTLRR151 pKa = 11.84EE152 pKa = 4.06EE153 pKa = 4.26EE154 pKa = 4.74LVTKK158 pKa = 9.9STGFPLNLPEE168 pKa = 5.46PIRR171 pKa = 11.84LYY173 pKa = 11.13LSGLGNVVTKK183 pKa = 10.66AGEE186 pKa = 4.05HH187 pKa = 6.12LKK189 pKa = 9.24PTFPEE194 pKa = 4.4LPTFIAAGSPGYY206 pKa = 10.16YY207 pKa = 9.89GQGPINQVSHH217 pKa = 6.41NLYY220 pKa = 10.85EE221 pKa = 4.71EE222 pKa = 4.69IPCLGTSITMIRR234 pKa = 11.84SSLSQIHH241 pKa = 6.77PLPQPICPVQPIGFTANEE259 pKa = 4.04NLCFWHH265 pKa = 6.76HH266 pKa = 4.08VHH268 pKa = 6.89KK269 pKa = 10.31PRR271 pKa = 11.84SEE273 pKa = 3.82ATDD276 pKa = 3.33ILRR279 pKa = 11.84SAGITEE285 pKa = 4.26INFPCSPTGTGFNFTLLKK303 pKa = 10.57SISALIANTSTFKK316 pKa = 9.85ITSVDD321 pKa = 3.3HH322 pKa = 7.0SITPEE327 pKa = 3.78SGAIAQTVITRR338 pKa = 11.84PTANNDD344 pKa = 2.86QVARR348 pKa = 11.84SVTAEE353 pKa = 3.97VEE355 pKa = 4.47PTCLNQEE362 pKa = 4.06ANGAFGMAIAFGYY375 pKa = 10.03QLFKK379 pKa = 11.29EE380 pKa = 4.35PFEE383 pKa = 4.33GVNTVWCCVTPNDD396 pKa = 4.38PDD398 pKa = 3.73VPIPEE403 pKa = 4.26DD404 pKa = 2.97WVLNRR409 pKa = 11.84NQRR412 pKa = 11.84RR413 pKa = 11.84NIPGEE418 pKa = 3.72YY419 pKa = 9.82FLRR422 pKa = 11.84KK423 pKa = 8.97FAAVSQDD430 pKa = 3.02AGDD433 pKa = 3.95FRR435 pKa = 11.84ILTTKK440 pKa = 11.05AMIKK444 pKa = 8.92TKK446 pKa = 10.51RR447 pKa = 3.35

Molecular weight: 49.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1994

447

576

498.5

56.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.222 ± 0.57

1.304 ± 0.282

6.419 ± 0.87

6.319 ± 0.128

4.614 ± 0.187

4.664 ± 0.433

2.808 ± 0.206

6.068 ± 0.622

5.567 ± 0.342

8.175 ± 0.536

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.285

3.912 ± 0.556

6.871 ± 0.981

3.511 ± 0.432

6.82 ± 0.778

6.57 ± 0.146

6.82 ± 0.633

5.366 ± 0.268

1.103 ± 0.291

3.811 ± 0.764
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski