Tenacibaculum sp. Bg11-29
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3926 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N1FCL0|A0A2N1FCL0_9FLAO MarR family transcriptional regulator OS=Tenacibaculum sp. Bg11-29 OX=2058306 GN=CXF68_19065 PE=4 SV=1
MM1 pKa = 7.56 GNLKK5 pKa = 10.41 RR6 pKa = 11.84 SIKK9 pKa = 10.04 RR10 pKa = 11.84 FKK12 pKa = 10.96 LSVLFLTLSLIYY24 pKa = 10.77 SNNSWAQQFYY34 pKa = 7.86 PTNFDD39 pKa = 3.37 VTTLNGTNGFVVPGLVTNYY58 pKa = 10.54 KK59 pKa = 10.12 LGRR62 pKa = 11.84 EE63 pKa = 4.15 VQFIGDD69 pKa = 3.61 INNDD73 pKa = 3.3 GFEE76 pKa = 5.24 DD77 pKa = 3.42 ICLGNGNQTVGVNDD91 pKa = 4.06 LSGRR95 pKa = 11.84 AYY97 pKa = 9.78 IIFGASTGFSTPFDD111 pKa = 3.75 LTSLNGTNGFIVEE124 pKa = 4.75 GIGYY128 pKa = 8.57 DD129 pKa = 3.55 EE130 pKa = 4.66 RR131 pKa = 11.84 RR132 pKa = 11.84 GTTVAGPGDD141 pKa = 3.84 INGDD145 pKa = 3.95 GIDD148 pKa = 4.28 DD149 pKa = 5.35 LIIGTSSSSSDD160 pKa = 3.22 DD161 pKa = 3.22 MVIYY165 pKa = 8.51 GTTTFPALLKK175 pKa = 10.0 VTDD178 pKa = 3.65 INGANGFLLDD188 pKa = 4.06 TPGSYY193 pKa = 9.5 QVASLGDD200 pKa = 3.62 VNGDD204 pKa = 4.0 SINDD208 pKa = 4.24 FIIGTPHH215 pKa = 7.31 WSGQSWIVFGRR226 pKa = 11.84 TSNYY230 pKa = 8.0 PALVDD235 pKa = 3.5 ATWINNAVNGFRR247 pKa = 11.84 TSSFPTSRR255 pKa = 11.84 PSLKK259 pKa = 10.71 VGGAGDD265 pKa = 3.86 INNDD269 pKa = 4.16 GINDD273 pKa = 3.68 ILIGGWNNSANPNSYY288 pKa = 11.42 ALFGKK293 pKa = 7.95 NTSFDD298 pKa = 3.43 AVVNLVTVDD307 pKa = 3.44 GTDD310 pKa = 3.14 GFLIDD315 pKa = 4.18 NEE317 pKa = 4.4 GNSFITEE324 pKa = 4.05 VGPLGDD330 pKa = 4.36 INNDD334 pKa = 4.01 GIDD337 pKa = 3.55 DD338 pKa = 4.23 CFSEE342 pKa = 4.21 NNIIFGSTSIFPANLLMSNFNGTNGFVLEE371 pKa = 4.55 NYY373 pKa = 9.51 VGSAAPTGDD382 pKa = 3.5 VNFDD386 pKa = 4.1 GIDD389 pKa = 3.49 DD390 pKa = 5.01 FIVAGADD397 pKa = 3.43 DD398 pKa = 4.14 YY399 pKa = 12.12 VVFGKK404 pKa = 8.81 TGGFSPTFNSTAIDD418 pKa = 3.72 GANGFKK424 pKa = 10.29 IPLIGSGGGGSVDD437 pKa = 3.32 GGKK440 pKa = 9.99 DD441 pKa = 3.44 VNGDD445 pKa = 4.05 GITDD449 pKa = 4.6 FIFADD454 pKa = 3.93 TGAGSTGEE462 pKa = 4.21 VYY464 pKa = 10.75 VVFGGDD470 pKa = 3.43 HH471 pKa = 6.05 YY472 pKa = 12.02 AMPLNATHH480 pKa = 6.65 PQANNEE486 pKa = 4.18 TTTGFDD492 pKa = 2.99 ILVNGPEE499 pKa = 4.38 TGTIHH504 pKa = 6.65 YY505 pKa = 9.57 VILPGTFSGAVNHH518 pKa = 5.47 NTILTGTGAIANGNFLMSTANANINEE544 pKa = 4.55 VISSLTAATTYY555 pKa = 10.52 DD556 pKa = 3.13 VHH558 pKa = 8.23 LFLEE562 pKa = 4.77 DD563 pKa = 4.17 GIGNKK568 pKa = 10.2 SIIYY572 pKa = 9.47 HH573 pKa = 6.38 LNNVTTLSAGDD584 pKa = 3.63 ITPPSITCPANQTVTCGTTVIPDD607 pKa = 3.37 YY608 pKa = 10.44 TGLVTTTDD616 pKa = 3.18 IVDD619 pKa = 4.15 PSPVVTQSPVAGAAFVSGMIITMTSTDD646 pKa = 3.16 ASLNSASCTFNVTVGGDD663 pKa = 3.56 NVKK666 pKa = 8.94 PTITCLISEE675 pKa = 4.46 SLFINSTLPNYY686 pKa = 9.29 FFKK689 pKa = 11.14 LKK691 pKa = 10.83 ASDD694 pKa = 3.43 NCTSIFNLTYY704 pKa = 9.66 TQVPAQGTVFTANTNVTVVVTDD726 pKa = 3.69 EE727 pKa = 4.22 NGNTEE732 pKa = 3.98 TCTFLVTVKK741 pKa = 10.71 GATPPVNCNTTSVNINDD758 pKa = 4.37 LDD760 pKa = 4.06 GDD762 pKa = 3.83 NGFQIDD768 pKa = 3.77 GDD770 pKa = 4.1 AVKK773 pKa = 10.5 AQLGHH778 pKa = 6.26 DD779 pKa = 3.55 VRR781 pKa = 11.84 GAGDD785 pKa = 3.48 VNGDD789 pKa = 4.35 GIQDD793 pKa = 3.83 FLVGSPNKK801 pKa = 10.25 AYY803 pKa = 10.64 NYY805 pKa = 7.91 NAAYY809 pKa = 9.9 GRR811 pKa = 11.84 EE812 pKa = 3.91 RR813 pKa = 11.84 GNFPGNVFVVFGKK826 pKa = 10.93 GNNFLPNIDD835 pKa = 3.82 TVLLDD840 pKa = 3.53 GTDD843 pKa = 3.15 GFKK846 pKa = 9.99 IHH848 pKa = 6.87 NDD850 pKa = 2.88 IPNGGSQYY858 pKa = 11.1 SGYY861 pKa = 9.97 QVSTAGDD868 pKa = 3.58 INNDD872 pKa = 4.12 GIDD875 pKa = 4.17 DD876 pKa = 4.71 LMFSDD881 pKa = 4.45 PFRR884 pKa = 11.84 RR885 pKa = 11.84 NPLGSEE891 pKa = 4.02 LGTIYY896 pKa = 11.03 VVFGKK901 pKa = 7.45 TTGFLPVFNLSNINGTNGFVFLGTDD926 pKa = 3.94 AYY928 pKa = 10.33 DD929 pKa = 3.52 QSGKK933 pKa = 10.85 SIDD936 pKa = 3.7 SAGDD940 pKa = 3.39 INNDD944 pKa = 4.02 GINDD948 pKa = 4.4 IIIGASTGRR957 pKa = 11.84 GTNNSGTCYY966 pKa = 10.4 VIYY969 pKa = 10.08 GKK971 pKa = 8.7 TGSFPALVRR980 pKa = 11.84 GSDD983 pKa = 3.73 LNGTNGFIIKK993 pKa = 10.23 GDD995 pKa = 3.57 TDD997 pKa = 4.07 GEE999 pKa = 4.3 QIGYY1003 pKa = 9.83 GVVGLGDD1010 pKa = 3.68 VNGDD1014 pKa = 3.95 GIDD1017 pKa = 3.64 DD1018 pKa = 4.41 LGFEE1022 pKa = 4.63 GGGKK1026 pKa = 9.95 KK1027 pKa = 8.25 KK1028 pKa = 9.39 TYY1030 pKa = 10.92 VLFGKK1035 pKa = 8.55 TGSFPAIVATATINGTNGFYY1055 pKa = 11.08 ADD1057 pKa = 3.44 ISSFTSSYY1065 pKa = 9.31 YY1066 pKa = 10.4 RR1067 pKa = 11.84 SITGVGDD1074 pKa = 3.44 VNNDD1078 pKa = 3.85 GIKK1081 pKa = 11.04 DD1082 pKa = 3.51 MLFGNRR1088 pKa = 11.84 FLLFGRR1094 pKa = 11.84 NTGFTTAEE1102 pKa = 5.04 DD1103 pKa = 3.39 ITNLDD1108 pKa = 3.86 GIKK1111 pKa = 10.09 GVEE1114 pKa = 4.88 FIGSTYY1120 pKa = 10.29 RR1121 pKa = 11.84 KK1122 pKa = 9.52 SSAGGDD1128 pKa = 3.8 FNNDD1132 pKa = 3.72 GIDD1135 pKa = 3.5 DD1136 pKa = 5.18 LIIGRR1141 pKa = 11.84 SSSSIAIVYY1150 pKa = 8.36 GKK1152 pKa = 8.36 STPWDD1157 pKa = 3.24 ATLNPFSLPSSEE1169 pKa = 4.05 AFKK1172 pKa = 10.68 VTGLSSDD1179 pKa = 3.75 YY1180 pKa = 11.02 SVSDD1184 pKa = 3.61 LADD1187 pKa = 3.3 INGDD1191 pKa = 3.71 GITDD1195 pKa = 3.81 VIVGEE1200 pKa = 4.27 KK1201 pKa = 10.02 EE1202 pKa = 3.44 RR1203 pKa = 11.84 LYY1205 pKa = 10.93 YY1206 pKa = 10.57 DD1207 pKa = 3.44 SSTSLNPGRR1216 pKa = 11.84 ANVIYY1221 pKa = 9.54 GFSVIDD1227 pKa = 3.48 TEE1229 pKa = 5.19 APVITCPIDD1238 pKa = 3.29 QVLVSGSLLPDD1249 pKa = 4.23 YY1250 pKa = 10.79 ISLATVTDD1258 pKa = 3.62 NCDD1261 pKa = 3.2 TSPVITQAPVAGGVYY1276 pKa = 9.17 TPGMTITLTATDD1288 pKa = 3.95 KK1289 pKa = 11.12 KK1290 pKa = 11.0 GNSANCDD1297 pKa = 4.08 FIVTDD1302 pKa = 3.62 NTDD1305 pKa = 3.76 TIGPTATNPLPINVQCLDD1323 pKa = 4.51 DD1324 pKa = 4.4 VPLPNPLVVTDD1335 pKa = 3.95 EE1336 pKa = 4.78 ADD1338 pKa = 3.22 NSGIPPTVAFVSDD1351 pKa = 4.15 VNTSPSVITRR1361 pKa = 11.84 TYY1363 pKa = 10.91 SVTDD1367 pKa = 3.62 GAGNTINVTQTITISDD1383 pKa = 4.38 TIKK1386 pKa = 9.61 PTATNPLPINVSCIGDD1402 pKa = 3.77 VPTADD1407 pKa = 2.77 ITVVTDD1413 pKa = 3.4 EE1414 pKa = 5.24 ADD1416 pKa = 3.43 NCTGTITIAFVSDD1429 pKa = 3.73 VNTSPGLITRR1439 pKa = 11.84 TYY1441 pKa = 10.87 SVTDD1445 pKa = 3.38 VAGNTVDD1452 pKa = 3.29 VTQTINISDD1461 pKa = 4.38 TIKK1464 pKa = 9.66 PTATNPLPINISCIGDD1480 pKa = 3.64 VPTADD1485 pKa = 2.8 ITVVSDD1491 pKa = 4.8 EE1492 pKa = 4.71 IDD1494 pKa = 3.42 NCTGTITVAFVSDD1507 pKa = 4.16 VNTSPSVITRR1517 pKa = 11.84 TYY1519 pKa = 10.86 SVTDD1523 pKa = 3.33 VAGNTINVTQIINVSDD1539 pKa = 3.42 IVKK1542 pKa = 8.72 PTASDD1547 pKa = 3.71 PLPISISCIGDD1558 pKa = 3.26 VPAADD1563 pKa = 3.04 ITVVTDD1569 pKa = 4.66 EE1570 pKa = 5.07 IDD1572 pKa = 3.37 NCTGTIIVAFVSDD1585 pKa = 4.19 VNTSPGLITRR1595 pKa = 11.84 TYY1597 pKa = 10.79 SVTDD1601 pKa = 3.42 AAGNTINVTQTINVSDD1617 pKa = 3.67 IVKK1620 pKa = 8.72 PTASDD1625 pKa = 3.65 PLPINVSCAGDD1636 pKa = 3.66 IPTADD1641 pKa = 2.95 ITVVTDD1647 pKa = 3.4 EE1648 pKa = 5.24 ADD1650 pKa = 3.39 NCTGTIIVAFVSDD1663 pKa = 4.22 VNTSPGIITRR1673 pKa = 11.84 TYY1675 pKa = 10.88 SVTDD1679 pKa = 3.42 AAGNTINVTQTINVSDD1695 pKa = 3.67 IVKK1698 pKa = 8.72 PTASDD1703 pKa = 3.65 PLPINVSCAGDD1714 pKa = 3.56 IPAADD1719 pKa = 3.2 ITVVIDD1725 pKa = 3.78 EE1726 pKa = 5.24 ADD1728 pKa = 3.49 NCTGAIIVAFVSDD1741 pKa = 4.22 VNTSPGIITRR1751 pKa = 11.84 TYY1753 pKa = 10.88 SVTDD1757 pKa = 3.45 AAGNTIDD1764 pKa = 3.44 VTQTINVSDD1773 pKa = 3.67 IVKK1776 pKa = 8.72 PTASDD1781 pKa = 3.67 PLPINISCIGDD1792 pKa = 3.64 VPTADD1797 pKa = 2.77 ITVVTDD1803 pKa = 3.4 EE1804 pKa = 5.19 ADD1806 pKa = 3.48 NCTGAIMVAFVSDD1819 pKa = 4.05 VNTSSSIITRR1829 pKa = 11.84 TYY1831 pKa = 10.93 SVTDD1835 pKa = 3.42 AAGNTINVTQTINVSDD1851 pKa = 4.46 IIKK1854 pKa = 8.71 PTASDD1859 pKa = 3.92 PLPINISCIGDD1870 pKa = 3.87 LPVADD1875 pKa = 3.52 ITVVTDD1881 pKa = 3.4 EE1882 pKa = 5.19 ADD1884 pKa = 3.43 NCTGAITVAFVSDD1897 pKa = 4.04 VNTSSSVITRR1907 pKa = 11.84 TYY1909 pKa = 10.89 SVTDD1913 pKa = 3.3 VAGNSINVTQTINVSDD1929 pKa = 4.46 IIKK1932 pKa = 8.71 PTASDD1937 pKa = 3.92 PLPINISCIGDD1948 pKa = 3.59 VPVADD1953 pKa = 3.32 ITVVTDD1959 pKa = 3.74 AADD1962 pKa = 3.42 NCTGVITVAFVSDD1975 pKa = 4.16 VNTSPSVIIRR1985 pKa = 11.84 TYY1987 pKa = 11.0 SVTDD1991 pKa = 3.31 VAGNSINVTQTITVSDD2007 pKa = 4.69 TISPTATNPLPINVLCTGDD2026 pKa = 4.39 IPTADD2031 pKa = 3.02 ISAITDD2037 pKa = 3.36 EE2038 pKa = 5.09 ADD2040 pKa = 3.0 NCTGVITVAFVNDD2053 pKa = 3.91 MNTSPGVITRR2063 pKa = 11.84 TYY2065 pKa = 10.86 SVTDD2069 pKa = 3.39 AAGNSTDD2076 pKa = 3.28 VTQTITVSDD2085 pKa = 5.02 IIPPTATNPAAIIISCVGDD2104 pKa = 3.86 IPAADD2109 pKa = 3.33 ITVVAGEE2116 pKa = 4.24 VDD2118 pKa = 3.5 NCTGTITVAFVSDD2131 pKa = 3.77 VSDD2134 pKa = 3.89 GNSNPEE2140 pKa = 4.16 IITRR2144 pKa = 11.84 TYY2146 pKa = 11.09 SVTDD2150 pKa = 3.35 AVGNFTNVTQTIIVNDD2166 pKa = 3.55 STAPITTCPSNSTQKK2181 pKa = 10.27 VDD2183 pKa = 3.42 SGLLTAVVTFTNPVATDD2200 pKa = 3.17 NCTVATINQTAGLPSGSEE2218 pKa = 3.91 FPIGVNTISFEE2229 pKa = 4.19 ATDD2232 pKa = 3.63 AAGNTATCSFTITIEE2247 pKa = 4.22 AEE2249 pKa = 4.07 DD2250 pKa = 4.35 PLVCTVDD2257 pKa = 2.98 AGTDD2261 pKa = 3.69 FDD2263 pKa = 5.08 IIEE2266 pKa = 4.42 GDD2268 pKa = 3.75 EE2269 pKa = 4.16 VALNAVISGAGVIEE2283 pKa = 4.3 WSPSVGVSSTTIANPIASPTEE2304 pKa = 3.87 TTTYY2308 pKa = 10.01 TIRR2311 pKa = 11.84 FVNAEE2316 pKa = 3.92 GCVAEE2321 pKa = 4.2 DD2322 pKa = 4.07 TITVFVTPLEE2332 pKa = 4.19 KK2333 pKa = 10.94 DD2334 pKa = 3.03 EE2335 pKa = 4.37 TKK2337 pKa = 11.01 YY2338 pKa = 11.1 GFSPDD2343 pKa = 3.05 GDD2345 pKa = 4.23 GINEE2349 pKa = 3.8 YY2350 pKa = 10.29 WEE2352 pKa = 3.84 IDD2354 pKa = 3.87 GIEE2357 pKa = 4.31 NYY2359 pKa = 10.01 PNNQVLIYY2367 pKa = 10.32 NRR2369 pKa = 11.84 WGDD2372 pKa = 4.47 LIFEE2376 pKa = 4.39 TTGYY2380 pKa = 10.9 NNASNVFRR2388 pKa = 11.84 GIANKK2393 pKa = 9.61 KK2394 pKa = 9.23 RR2395 pKa = 11.84 NLGAGKK2401 pKa = 10.03 LPEE2404 pKa = 4.25 GTYY2407 pKa = 10.65 LFHH2410 pKa = 8.07 IKK2412 pKa = 10.12 IKK2414 pKa = 10.54 GSHH2417 pKa = 5.32 NLKK2420 pKa = 10.12 KK2421 pKa = 10.79 EE2422 pKa = 3.73 KK2423 pKa = 10.68 GFLILKK2429 pKa = 9.86 RR2430 pKa = 3.77
Molecular weight: 252.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.666
IPC2_protein 3.706
IPC_protein 3.783
Toseland 3.528
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.427
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.215
Thurlkill 3.592
EMBOSS 3.757
Sillero 3.91
Patrickios 1.481
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|A0A2N1FBU1|A0A2N1FBU1_9FLAO 3-isopropylmalate dehydratase large subunit OS=Tenacibaculum sp. Bg11-29 OX=2058306 GN=leuC PE=3 SV=1
MM1 pKa = 8.03 PKK3 pKa = 8.97 RR4 pKa = 11.84 TYY6 pKa = 10.33 QPSKK10 pKa = 9.01 RR11 pKa = 11.84 KK12 pKa = 9.47 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.03 GRR40 pKa = 11.84 KK41 pKa = 7.95 NLSVSSAPRR50 pKa = 11.84 PKK52 pKa = 10.38 KK53 pKa = 10.5
Molecular weight: 6.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.921
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.003
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3926
0
3926
1305142
35
7359
332.4
37.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.707 ± 0.042
0.783 ± 0.019
5.174 ± 0.042
6.397 ± 0.048
5.301 ± 0.04
6.001 ± 0.049
1.652 ± 0.02
8.623 ± 0.044
9.109 ± 0.091
9.182 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.931 ± 0.022
6.946 ± 0.04
3.043 ± 0.025
3.07 ± 0.022
3.029 ± 0.025
6.709 ± 0.038
6.212 ± 0.087
6.004 ± 0.046
1.001 ± 0.014
4.126 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here