Streptococcus satellite phage Javan114

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZDH5|A0A4D5ZDH5_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan114 OX=2558528 GN=JavanS114_0016 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.27IKK4 pKa = 10.3IFYY7 pKa = 8.32QQQNQSLQEE16 pKa = 4.0FEE18 pKa = 4.69AQINNFMASVEE29 pKa = 4.24VVDD32 pKa = 5.09VKK34 pKa = 10.66CTEE37 pKa = 4.01ATEE40 pKa = 4.13GDD42 pKa = 4.36YY43 pKa = 11.64EE44 pKa = 4.22NLSATLGLLVLYY56 pKa = 10.69KK57 pKa = 10.48EE58 pKa = 4.5

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZFT1|A0A4D5ZFT1_9VIRU PriCT_1 domain-containing protein OS=Streptococcus satellite phage Javan114 OX=2558528 GN=JavanS114_0008 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 9.33ITEE5 pKa = 4.22VKK7 pKa = 10.53KK8 pKa = 10.48KK9 pKa = 10.5DD10 pKa = 3.31GSNVYY15 pKa = 9.72RR16 pKa = 11.84ASVYY20 pKa = 10.78LGVDD24 pKa = 2.9QVTGKK29 pKa = 10.15KK30 pKa = 10.12VKK32 pKa = 9.8TSITGRR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 8.72EE42 pKa = 4.07VKK44 pKa = 10.28SKK46 pKa = 10.67AQHH49 pKa = 5.72AQLDD53 pKa = 4.67FKK55 pKa = 11.77ANGSTVHH62 pKa = 5.11KK63 pKa = 8.56TVRR66 pKa = 11.84VKK68 pKa = 10.74NYY70 pKa = 10.28QEE72 pKa = 4.72LAEE75 pKa = 4.75LWLEE79 pKa = 4.21SYY81 pKa = 10.9RR82 pKa = 11.84LTVKK86 pKa = 10.03PQTFIATEE94 pKa = 3.93RR95 pKa = 11.84MVNSHH100 pKa = 7.68LIPVFGKK107 pKa = 9.06MRR109 pKa = 11.84LDD111 pKa = 3.4KK112 pKa = 10.95LAASYY117 pKa = 10.16IQSFINDD124 pKa = 3.53LSSRR128 pKa = 11.84LVHH131 pKa = 6.43FGVVHH136 pKa = 6.77SINSRR141 pKa = 11.84ILKK144 pKa = 10.27YY145 pKa = 10.48GVSLQLLPFNPARR158 pKa = 11.84DD159 pKa = 3.52IILPRR164 pKa = 11.84KK165 pKa = 8.83PKK167 pKa = 10.33RR168 pKa = 11.84EE169 pKa = 3.83SKK171 pKa = 10.3AIKK174 pKa = 10.18FIAPEE179 pKa = 4.14DD180 pKa = 3.8LKK182 pKa = 11.58SLLGYY187 pKa = 8.07MEE189 pKa = 4.55KK190 pKa = 10.67LAFKK194 pKa = 10.28KK195 pKa = 10.07YY196 pKa = 9.29SHH198 pKa = 6.33YY199 pKa = 10.57FDD201 pKa = 4.03YY202 pKa = 11.26VLYY205 pKa = 10.72SLLLATGCRR214 pKa = 11.84FGEE217 pKa = 4.29AVALEE222 pKa = 4.2WSDD225 pKa = 3.65IDD227 pKa = 5.39LEE229 pKa = 4.22NATISISKK237 pKa = 10.02NYY239 pKa = 9.17NRR241 pKa = 11.84LVDD244 pKa = 4.31LVGPPKK250 pKa = 10.52SKK252 pKa = 10.78AGVRR256 pKa = 11.84VISIDD261 pKa = 3.3RR262 pKa = 11.84KK263 pKa = 7.22TVNMLKK269 pKa = 10.23LYY271 pKa = 10.65KK272 pKa = 10.14NRR274 pKa = 11.84QRR276 pKa = 11.84QLFVEE281 pKa = 4.94DD282 pKa = 4.01GARR285 pKa = 11.84VPSVVFAIPTKK296 pKa = 10.24AYY298 pKa = 10.59QNMATRR304 pKa = 11.84QDD306 pKa = 3.65SLDD309 pKa = 3.68RR310 pKa = 11.84RR311 pKa = 11.84CKK313 pKa = 9.94EE314 pKa = 3.14IRR316 pKa = 11.84IPRR319 pKa = 11.84FTFHH323 pKa = 7.65AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.49RR346 pKa = 11.84LGHH349 pKa = 6.19ATLAMTMDD357 pKa = 4.24IYY359 pKa = 11.79SHH361 pKa = 6.95LSKK364 pKa = 10.92DD365 pKa = 3.51KK366 pKa = 10.41EE367 pKa = 4.25KK368 pKa = 10.93EE369 pKa = 3.85AVSYY373 pKa = 9.52YY374 pKa = 10.59EE375 pKa = 4.03KK376 pKa = 10.7AINGLL381 pKa = 3.67

Molecular weight: 43.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

3359

41

490

197.6

22.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.639 ± 0.545

0.685 ± 0.196

5.061 ± 0.22

8.276 ± 0.511

4.376 ± 0.449

5.15 ± 0.334

1.786 ± 0.234

6.847 ± 0.421

8.247 ± 0.328

9.527 ± 0.458

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.221

5.001 ± 0.658

3.96 ± 0.408

3.602 ± 0.484

5.567 ± 0.565

6.133 ± 0.493

6.103 ± 0.422

5.656 ± 0.584

0.774 ± 0.184

4.317 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski